Objectives To establish a rapid analytical procedure for salmonella in textiles by LAMP in combination with MALDI TOF MS. The gene sequence invA of salmonella is specific to pathogenic strains. Based on its characteri...Objectives To establish a rapid analytical procedure for salmonella in textiles by LAMP in combination with MALDI TOF MS. The gene sequence invA of salmonella is specific to pathogenic strains. Based on its characteristic,a set of LAMP primer for salmonella is designed. If the amplification result is suspicious or positive,the strains are separated and the determination is rapidly performed through MALDI TOF MS technique. Methods About 70 reference strains closely related to the textiles and public health are selected to perform the specificity study(Salmonella enterica,Salmonella tyhimurium,Salmonella choleraesuis,Salmonella paratyphi A etc.);The bacterium solution of salmonella is diluted to different gradients for the sensitivity study. Results The results show this method is appropriately specific and sensitive,the limit of detection up to 4.6 CFU/mL. Conclusions The procedure is a new technique to detect salmonella in textiles.展开更多
基质辅助激光解吸电离飞行时间质谱(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术是临床微生物诊断中的一项革命性创新技术,给临床微生物诊断带来了极大的便利,同时也给临床微生...基质辅助激光解吸电离飞行时间质谱(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术是临床微生物诊断中的一项革命性创新技术,给临床微生物诊断带来了极大的便利,同时也给临床微生物实习带教带来了新的挑战。本文阐述了MALDI-TOF MS技术在临床微生物检验实习带教中的应用探讨,旨在充分运用MALDI-TOF MS技术结合传统鉴定技术,提高临床微生物检验实习的教学能力。展开更多
文摘Objectives To establish a rapid analytical procedure for salmonella in textiles by LAMP in combination with MALDI TOF MS. The gene sequence invA of salmonella is specific to pathogenic strains. Based on its characteristic,a set of LAMP primer for salmonella is designed. If the amplification result is suspicious or positive,the strains are separated and the determination is rapidly performed through MALDI TOF MS technique. Methods About 70 reference strains closely related to the textiles and public health are selected to perform the specificity study(Salmonella enterica,Salmonella tyhimurium,Salmonella choleraesuis,Salmonella paratyphi A etc.);The bacterium solution of salmonella is diluted to different gradients for the sensitivity study. Results The results show this method is appropriately specific and sensitive,the limit of detection up to 4.6 CFU/mL. Conclusions The procedure is a new technique to detect salmonella in textiles.
文摘基质辅助激光解吸电离飞行时间质谱(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术是临床微生物诊断中的一项革命性创新技术,给临床微生物诊断带来了极大的便利,同时也给临床微生物实习带教带来了新的挑战。本文阐述了MALDI-TOF MS技术在临床微生物检验实习带教中的应用探讨,旨在充分运用MALDI-TOF MS技术结合传统鉴定技术,提高临床微生物检验实习的教学能力。
