The CFTR polymorphisms poly-T, TG-repeats and M470V in Chinese males with congenital bilateral absence of the vas deferens

Congenital bilateral absence of the vas deferens （CBAVD） is a frequent cause of obstructive azoospermia, and mutations of the cystic fibrosis transmembrane conductance regulator （CFTR） gene have also been frequently identified in patients with CBAVD. However, the distribution of the CFTR polymorphisms M470V, poly-T, TG-repeats and F508del mutation in the Chinese CBAVD population with presumed low cystic fibrosis （CF） frequency remains to be evaluated. Samples obtained from 109 Chinese infertile males with CBAVD and 104 normal controls were analyzed for the presence of CFTR （TG）m（T）n, M470V and F508del by PCR amplification followed by direct sequencing. Our study showed that the F5OSdel mutation was not found in our patients. The 5T mutation was present with high frequency in Chinese CBAVD patients and IVS8-5T linked to either 12 or 13 TG repeats was highly prevalent among CBAVD patients （97.22% of 72 cases and 96.91% of 97 alleles with IVS8-5T）. Moreover, a statistically significant relationship between TG 12-5T-V470 haplotype and CBAVD was detected. This study indicated that the CFTR polymorphisms poly-T, TG-repeats and M470V might affect the process of CBAVD in the Chinese population.

ALK Family Inhibitor A83-01 Promotes the Proliferation of Mouse Male Germline Stem Cells (mGSCs) Under Serum- and Feeder-Free Conditions

A83-01 is a selective inhibitor of the TGF-β type I receptor ALK,which inhibits the TGF-β-induced epithelial-to-mesenchymal transition（EMT） via the inhibition of Smad2 phosphorylation.Previous studies have showed that A83-01 promoted somatic cellular reprogramming significantly.Male germline stem cells（mGSCs）,as an alternative resource of pluripotent stem cells derived adult testis,have promising valuable in clinic medicine and regeneration,however,the derivation of mGSCs was complex and difficult.What the role A83-01 plays in promoting the proliferation of mGSCs is still unknown.In this study,combined with A83-01 and knockout serum replacement（KSR） medium,we obtained a relatively feeder-and serum-free system for mGSCs culturing in vitro and the optimal concentration of A83-01 was 0.25 μmol L-1.After continuous culturing,the proliferation efficiency of undifferentiated mGSCs and differentiation capacity of mGSC were examined as well.Results showed that,A83-01 dramatically increased the number of mGSCs and AP positive colonies,and the mitosis index according to the BrdU assay.A83-01 could also increase the expression of pluripotent markers including Oct4,Klf4,Nanog and c-Myc,analyzed byreal-time quantative PCR.mGSCs cultured in the optimal feeder-and serum-free system combined with A83-01 could form embryoid bodies（EBs）,which consisted of three embryonic layers detected by immunofluorescence and RT-PCR.Remarkably,the results demonstrated 0.25 μmol L-1A83-01 could promote the proliferation of mouse mGSC colonies and maintain their undifferentiated status under feeder-and serum-free systems.

Update summarising the conclusions of the international consultation on male lower urinary tract symptoms

The International Consultation on Urological Disease have recently published comprehensive conclusions, based on evidence reviewed by eight committees, on aspects of male lower urinary tract symptoms(LUTS). In this review, we summarise the conclusions from fourof the committees, namely, the evidence regarding the epidemiology of male LUTS, patient assessment, nocturia and medical management. It is indisputable that with an expanding and ageing global population the prevalence of male LUTS is likely to increase. Therefore symptom prevention and preservation of quality of life(Qo L) feature highly in the guidelines. There are now a number of different medical options, proven to lead to significant improvements in symptom scores, flow rate and Qo L available to men with LUTS. Metaanalyses have shown the benefits for alpha blockers, antimuscarinics, 5-α reductase and phosphodiesterase-5 inhibitors. High level evidence also exists for combinations of all of the above with alpha blockers and so men with concomitant storage symptoms, prostate volume > 30 mL, PSA > 1.4 or erectile dysfunction may be considered for combination treatment of an alpha blocker with an antimuscarinic, 5-α reductase inhibitor or phosphodiesterase-5 inhibitor respectively. In an era of personalised medicine, appropriate patient selection is likely to provide the key to the most effective clinical management strategy.

On AP-property of Function Spaces

In this paper, it is discussed the AP-property of function spaces. We prove that for any compact network α for a space X which is closed under finite unions, (1) if C α (X) is an AP-space and X is paracompact, then X is a Hurewicz space; (2) if C α (X) is an AP-space which has countable tightness, then C α (X) is discretely generated.

Effects of Garcinia kola “Bitter Kola” Powder on Sex Reversal, Survival and Growth Performance of Tilapia (Oreochromis niloticus L.)

This study was carried out in 2022 at the Itato agriculture community estate of fish hatchery in eastern Senegal with the aim of finding an alternative method to 17-α-methyl testosterone (MT) by using Garcinia kola “Bitter kola” powder to obtain Oreochromis niloticus male sex populations. 1800 fry of average size between 0.01 and 0.02 g were distributed in 12 aboveground tanks of 1 m3 each at a rate of 150 individuals/tank, following 6 duplicate treatments. The negative (B1 and B2) and positive (B3 and B4) control diets, to which between 0 and 60 mg/kg of 17alpha-methyl testosterone had been added respectively, were mixed with industrial feed containing 40% protein;the tank diets (B5 and B6), (B7 and B8), (B9 and B10) and (B11 and B12) contained 10 g, 20 g, 30 g and 40 g of Garcinia kola powder respectively for 1 kg of industrial feed con-taining 40% protein. These different treatments resulted in high proportions of males in the order of 97.03%;98.09% for the hormone and proportions of 94.03%;93.26% for 20 g of Garcinia kola extracts. In terms of weight growth, the best average weights for the hormone and Garcinia kola were 0.896 g and 0.980 g respectively. With regard to fish survival, the following results were recorded: 95.53% and 94.56% respectively for additions of 20 g and 30 g of Garcinia kola extracts to the feed supplied to the fish. On the other hand, a significant difference in the mortality rate of 35% and 5% respectively for the conventional hormone and 20 g of Garcinia kola extracts was observed. To conclude the study, it is recommended that further studies be carried out to determine the quality of the flesh, their life stages and the profile of the amino acids present in their proteins.

他们为什么选择了离开——学前教育专业男生毕业流失问题的背后

高校学前教育专业男生作为幼儿园男教师的储备人才受到社会各界的关注,但学前教育专业男生的毕业流失现象普遍存在。本研究运用半结构深度访谈,对3位学前教育专业毕业但未从事相关职业的男生进行了深入调查,试图探讨他们职业选择背后的原因。

北京市某高校男性大学生心理健康、共情与攻击的关系调查

目的:探究男性大学生心理健康、共情与攻击之间的关系。方法:通过症状自评量表SCL-90、人际反应指标和Buss攻击问卷对北京两高校413名男性大学生进行调查。结果:相关分析表明男性大学生的SCL-90除观点采择与共情关注无显著相关外,幻想、个人忧伤与SCL-90总分均呈显著正相关(r=0.233,P<0.05;r=0.215,P<0.05)。共情与攻击存在正相关(r=0.19,<0.01);SCL-90与攻击存在正相关(r=0.619,P<0.01)。结论:大学生的心理健康,观点采择和共情关注能力与攻击呈负向关系,而个人忧伤与攻击呈正相关。因此,在预防大学生攻击行为时,需要改善其心理健康水平,提高观点采择和共情关注能力,并减少其个人忧伤水平。

男性护理硕士研究生学习动机分析

目的:了解男性护理硕士研究生学习动机的特点,为高等护理教育实践提供依据。方法:纳入8名男性护理硕士研究生作为研究对象,进行半结构式个人深入访谈,收集资料并进行分析。结果:就业生活型、求知型学习动机是男性护理硕士研究生主要学习动机;报答型、成绩目标型、消极回避型学习动机是次要学习动机;社会奉献型学习动机偏低。结论:高等护理教育须根据男性护理硕士研究生的学习动机分布情况,在课程设置、教学方法等方面进行适当调整,以完善高等护理教育相关机制和内容。

从岩相古地理论中国南方中下三叠统油气潜景

中下三叠统是中国南方重要的含油气海相碳酸盐岩地层，油气潜力很大，但勘探程度在大部分地区却很低．在定量岩相古地理研究及编图的基础上，对中国南方中下三叠统的烃源岩和储集岩的类型、分布及其与沉积环境的关系，对区域盖层和局部盖层的性质和分布，尤其是对中下三叠统经过多次重大构造变动后所形成的油气保存单元等进行了较深入的研究．指出了５个最有利的油气勘探地区，即绵阳—广元、南充—达县、泸州—重庆、宜昌—沙市、安庆—芜湖地区，以及９个有利的勘探地区和１０个较有利的勘探地区，为我国南方油气勘探打下坚实的基础．

煤的导电机理研究

本文用南桐煤田煤样研究了煤的导电性，并初步探讨了煤的导电性与煤大分子结构的关系．本文的研究结果对于煤的导电特性的研究及煤的气化均有一定的参考价值：图２，表３，参５。

我国南方部分花岗岩风化壳中残留长石的表面结构

综合应用扫描电镜、透射电镜等技术手段，研究了我国热带、亚热带地区五个花岗岩风化壳中残留长石的表面结构特征．在风化作用过程中，长石晶体表面发生了显著的不均衡溶蚀，并形成多种富特征性的溶蚀痕迹；双晶接合面、解理面、条纹长石中的主、嵌晶界面、构造破裂面等部位是长石晶体上优先溶蚀的部位．风化残留长石表面结构特征形成的因素包括长石的结晶学性质、研究区的气温、降水条件及长石晶体被风化的程度．建议在沉积岩成岩作用等研究工作中，应注意陆源长石碎屑表面可能存在的风化溶蚀痕迹对有关研究结果的影响．

“龙”、“Klu”和“Dragon”——兼论翻译中词的文化不等值现象及处理

在英汉、藏汉翻译中,英语中的dragon和藏语中klu都被译作“龙”。实质上,龙、klu和dragon是不同民族长期文化积累的产物,互相之间没有形貌上的同一性和文化内涵上的类似性,所以将dragon和klu译成龙,是在翻译过程中忽略了三者之间文化不等值的缘故,是一种误译。

TWO NEW SPECIES AND TWO NEW RECORDS OF THE GENUS ETHMIA HUBNER (LEPIDOPTERA: OECOPHORIDAE) FROM CHINA

The present paper deals with two new ethmid moths from China: Ethmia damaoshanae sp.nov. and Ethmia jingdongensis sp.nov. Another two species Ethmia dodecea (Haworth) and Ethmia ultima Sattler are reported for the first time from the country. The genitalia of the new species are figured.

复杂断块油田特高含水期实现持续稳产的做法

胜利油田临盘采油厂目前开发动用临盘、商河、临南三个油田,区域构造位于渤海湾盆地济阳坳陷惠民凹陷中央隆起带。“九五”末期由于油田相继进入特高含水期,剩余油分布复杂、断块破碎、油水井套损严重、注采完善程度低,造成油田产量连续三年下降。针对严峻的开发形势,进入“十五”以后,通过深化油藏构造、储层、剩余油潜力的研究,强化注采井网的完善。提高了水驱控制程度,增强油田稳产基础,提高采收率。全厂连续三年原油年产量连续稳定在163×104t,取得较好的开发效果和经济效益。

Sequence analysis of the gene correlated with cytoplasmic male sterility (CMS) in rape-seed (Brassica napus) Polima and Shaan 2A

orf224 is a CMS-related mitochondrial gene discovered in Polima cytoplasm. Shaan 2A CMS line is the parent of the first rapeseed hybrid cultivar Qinyou No. 2 that has been grown in many regions of China. In this work, genomic DNA of Polima CMS line and Shaan 2A CMS line were used as templates, two primers of specific oligonucleo-tides at 5’ and 3’ ends were used, PCR was performed, the amplification fragments were cloned into pGEM-T Easy vectors and DNA sequences were determined. The CMS-associated gene, orf224-1 present in Shaan 2A CMS line, has a sequence highly homologous to the orf224 of the Polima CMS line, except for one nucleotidc at position +398. There were only one base (AAC→AGC) and one amino acid (Asn→Ser) differences between the two. The homologies of the two sequences in nucleotide and amino acid were 99.9% and 99.6%, respectively. It is concluded that orf224 in Polima CMS line and orf224-1 of Shaan 2A CMS line are the allele at the same locus in mitochondria.

新入职男护士转型冲击体验的质性研究

目的:了解新入职男护士面临转型冲击的体验,为护理管理者更有效地稳定男护士队伍,促进男护士团队的建设与男性护理事业发展提供参考。方法:采用质性研究中现象学研究方法对12名新入职男护士进行半结构式访谈,通过录音和笔记相结合的方法记录访谈内容,采用Colaizzi 7步分析法进行资料汇总分析,提炼主题。结果:通过对访谈资料分析、归纳出4个主题,包括主题一:新入职男护士转型冲击大、理论和实践有差距;主题二:寄予厚望,自觉专业知识及技能缺乏;主题三:自我调节和融入集体;主题四:临床、心理、情感多方面支持。结论:新入职男护士存在一定程度的转型冲击,对新入职男护士弱势领域培训课程的建立、个性化需求选择“一对一”带教老师、切实加强临床知识和技能,提供心理咨询、组建“男护士组织”团体等可以作为降低转型冲击的有效措施。

Testicular exosomes disturb the immunosuppressive phenotype of testicular macrophages mediated by miR-155-5p in uropathogenic Escherichia coli-induced orchitis

Male reproductive infections are known to shape the immunological homeostasis of the testes,leading to male infertility.However,the specific pathogenesis of these changes remains poorly understood.Exosomes released in the inflammatory microenvironment are important in communication between the local microenvironment and recipient cells.Here,we aim to identify the immunomodulatory properties of inflammatory testes-derived exosomes(IT-exos)and explore their underlying mechanisms in orchitis.IT-exos were isolated using a uropathogenic Escherichia coli(UPEC)-induced orchitis model and confirmed that IT-exos promoted proinflammatory M1 activation with increasing expression of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)in vitro.We further used small RNA sequencing to identify the differential miRNA profiles in exosomes and primary testicular macrophages(TMs)from normal and UPEC-infected testes,respectively,and identified that miR-155-5p was highly enriched in IT-exos and TMs from inflammatory testes.Further study of bone marrow derived macrophages(BMDMs)transfected with miR-155-5p mimic showed that macrophages polarized to proinflammatory phenotype.In addition,the mice that were administrated IT-exos showed remarkable activation of TM1-like macrophages;however,IT-exos with silencing miR-155-5p showed a decrease in proinflammatory responses.Overall,we demonstrate that miR-155-5p delivered by IT-exos plays an important role in the activation of TM1 in UPEC-induced orchitis.Our study provides a new perspective on the immunological mechanisms underlying inflammation-related male infertility.

Fine mapping of an Arabidopsis thaliana male sterile mutant EC2-157

An Arabidopsis thaliana male sterile mutant EC2-157 has been isolated using an EMS mutagenesis strategy.Genetic analysis indicated that it was controlled by a single recessive gene called ms157.No pollen grains have been observed in mutant anthers.ms157 Has been mapped to a region of 74 kb located in BAC clone T6K22 on chro-mosome IV using a map-based cloning strategy.As no male sterile genes have been reported in this region,ms157 could be a novel gene related to fertility.The further molecular cloning and functional analysis on this gene should facili-tate our understanding of A.thaliana anther development.

煤气发生炉的反求设计及其改进

煤气发生炉的反求设计及其改进韩敏（四川简阳轻工机械厂）１引言煤气发生炉是广泛应用于化工、机械、城市煤气的设备。近年来，随着国民经济的不断发展，国家对环境保护的要求越来越严。为了企业的生存，越来越多的制浆造纸企业将碱回收提到了议事日程上。已有碱回收工段...

Seed and Wasp Production in the Mutualism of Figs and Fig Wasps

Figs (Moracea: Ficus) and fig wasps (Hymenoptera: Chlocloids: Agaonideae) depend on each other to complete their reproduction. Monoecious fig species and their pollinating wasps are in conflict over the use of fig ovaries which can either produce one seed or one wasp. From observation on Ficus virens Ait., we showed that female flowers with outer layer of ovaries (near to the wall of syconium) had no significant difference from that with inner and interval layer of ovaries (near to the syconium cavity), in which most seeds and wasps were produced. This meant that fig tree provided the same potential resource for seed and wasps production. Observation indicated that there was usually only one foundress in syconium at female flower phase and no com- petition pollinators. Measurement of the style length of female flowers and the ovipositor of pollinators indicated that most ovaries could be reached by pollinator’s ovipositor. However, at the male flower phase, production of seeds was significantly more than that of wasps including non-pollinating wasps but there was no significant difference between seed and pollinating wasp production when without non-pollinating wasps produced. This result indicated that non-pollinating wasps competed ovaries not with seeds but with pollinating wasps for ovipositing. Bagged experiment showed that the sampling fig species was not self-sterile which was important for figs and wasps to survive bad season. Seed production in self-pollinated figs was not significantly different from total wasps in- cluding non-pollinating ones. This might be related with the weaker competition among wasps since bagged figs were not easy to reach by wasps from outside.