Oxidative reactions are key part of the stress response in marine organisms, which are exposed to a wide variety of environmental stressors. Lipid peroxidation (LPO) occurs in response to oxidant attack, giving rise t...Oxidative reactions are key part of the stress response in marine organisms, which are exposed to a wide variety of environmental stressors. Lipid peroxidation (LPO) occurs in response to oxidant attack, giving rise to unsaturated aldehydes as malondialdehyde (MDA), the main biomarker for LPO assessment. Levels of MDA are measured in biological samples of different fish matrices to determine the oxidative effect of physical or chemical agents, particularly represented by metals. The most used assay is the spectrophotometric determination of thiobarbituric acid (TBA) as MDA-TBA2 adduct. Selectivity is enhanced by separating the adduct by chromatographic methods such as high-performance liquid chromatography or by using alternative derivatization reagents. Because, in biological organism, MDA is found free or protein bound, the two forms should be measured, and the extraction procedures are a crucial step in the processing of the biological sample. This review focused on MDA determination procedures used to assess the effect of experimental and environmental stress induced in fish.展开更多
The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde（ MDA）content. At three-leaf and one-heart stage of Luliangyou 996,15 μg...The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde（ MDA）content. At three-leaf and one-heart stage of Luliangyou 996,15 μg/mL Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. The antioxidant enzyme（ SOD,POD,PPO,CAT and PAL） activities and malondialdehyde（ MDA） content in rice leaves were determined every 24 h within 168 h post spraying. Compared with CK,the MDA content and activities of the five antioxidant enzymes increased in Agantibiotic 702 treatment,Validamycin treatment and M. grisea inoculation treatment. The MDA content and SOD,CAT and POD activities in Ag-antibiotic 702 treatment were significantly higher than those in M. grisea inoculation treatment in early stage,and the APX and LOX activities increased more remarkably and rapidly. The induction effect of Ag-antibiotic 702 could reach or exceed that of Validamycin treatment. Ag-antibiotic 702 enhanced the resistance to rice blast because MDA content and antioxidant enzymes activities were improved in rice leaves. Therefore,the increase of rice blast resistance induced by Ag-antibiotic 702 in rice was positively correlated with the increase of MDA content and antioxidant enzymes activities in rice leaves.展开更多
Effect of Siqi decoction on myocardial ischemia is to prevent cardiac myocyte membrane from damage associated with oxygen free radicals related to NO. To research the regulatoin of the content of malondialdehyde by Si...Effect of Siqi decoction on myocardial ischemia is to prevent cardiac myocyte membrane from damage associated with oxygen free radicals related to NO. To research the regulatoin of the content of malondialdehyde by Siqi decoction, an index of lipid peroxidation, via increasing activity of superoxide dismutase in blood serum of rats with Myocardial Ischemia, the model of myocardium ischemia was made in Wistar rats with posterior pituitary injection through vein in tail. Siqi decoction, Diaoxinxuekang（DK） and Fufangdanshenpian（FD）, the latter two drugs of which are effective TCM drugs of anti-myocardial ischemia at present, were administrated to the rats with myocardium ischemia for 5 days to compare the effect of them on myocardium ischemia as reference drugs via measuring the changes of the content of malondialdehyde and the activity of superoxide dismutase in the rat blood serum with myocardial ischemia. There were a remarkable increase in the activity of superoxide dismutase and a decrease in the content of malondialdehyde in the serum of the rats administered Siqi decoction compared with those of the rats in control group, p〈0.05. The contents of MDA in the serum of the prevention group rats in the experiments are lower than those of the cure group rats. Anti-Myocardium Ischemia mechanism of Siqi decoction is the regulation of the content of malondialdehyde via increasing activity of superoxide dismutase in the serum of Rats with myocardial ischemia and stimulating the activity of NOS in serum so as to increase NO concentration.展开更多
Background and objectives: Diabetes is a chronic multifactorial disease which requires a variety of strategies to reduce its epidemic. Type 2 diabetes (T2MD) is the most common form of diabetes which results from inef...Background and objectives: Diabetes is a chronic multifactorial disease which requires a variety of strategies to reduce its epidemic. Type 2 diabetes (T2MD) is the most common form of diabetes which results from inefficiency of insulin secretion or resistance to insulin action, both of which lead to chronic hyperglycemia. Lipid peroxidation is an oxidative stress process that involve in T2DM complications. This study aimed to 1) determine plasma malondialdehyde (MDA) levels as a biomarker for lipid peroxidation in Sudanese patients with T2DM, and 2) assess the associations between MDA and diabetes-related variables. Material and Methods: This case-control study was conducted from November 2022 to June 2023 at the National University, Sudan. It included 100 participants, of whom 50 were T2DM patients and 50 were healthy controls. Data on demographics, clinical characteristics, and biochemical markers (FBS, HbA1c, and MDA) were collected. The NycoCard HbA1c method and the GOD-POD Method were used for HbA1c and glucose measurement, respectively. MDA was determined by the thiobarbituric acid reactive substances method. The data were analyzed using SPSS Version. Results: Significant differences were observed between T2DM patients and healthy controls in FBS (P = 0.000), HbA1c (P = 0.000), and MDA (P = 0.010), with T2DM patients exhibiting higher levels. The study revealed a strong positive correlation between MDA levels and the duration of diabetes (r = 0.69, P = 0.00), while other variables, including age, BMI, and glucose control, did not significantly correlate with MDA levels. Conclusion: Findings revealed elevated MDA levels in Sudanese T2DM patients, with a positive correlation between MDA and diabetes duration. These findings emphasize the importance of oxidative stress in T2DM pathogenesis and call for the need for targeted strategies to mitigate oxidative damage and improve diabetes care.展开更多
BACKGROUND： Studies have confirmed that basic fibroblast growth factor （bFGF） promotes neuronal survival and neurite outgrowth. OBJECTIVE： To compare and verify the effects of bFGF on superoxide dismutase activity...BACKGROUND： Studies have confirmed that basic fibroblast growth factor （bFGF） promotes neuronal survival and neurite outgrowth. OBJECTIVE： To compare and verify the effects of bFGF on superoxide dismutase activity and malondialdehyde content in rat brain tissues surrounding a hemorrhagic lesion, as well as the hippocampus at the hemorrhagic side. DESIGN, TIME AND SETTING： The randomized, controlled, neurobiological study was performed at the Science Experimental Center and Research Laboratory, Guangxi Medical University, China, from September to December 2006. MATERIALS： Ninety-two adult, healthy, Wistar rats of equal gender were used to establish intracerebral hemorrhage by infusing type VII collagenase into the left internal capsule. Type Ⅶ collagenase （Sigma, USA）, superoxide dismutase and malondialdehyde kits （Jiancheng, China）, and bFGF （Institute of Bioengineering, Ji＇nan University, China） were used for this study. METHODS： Ninety successfully lesioned rats were equally and randomly divided into three groups. Rats in the bFGF group were intramuscularly injected daily with bFGF （8 ug/kg）. Rats in the saline control group received an equal volume of saline. The rats in the model group did not receive other interventions. Superoxide dismutase activity was measured using the xanthine oxidase method. Malondialdehyde contents were detected using the thiobarbituric acid method. MAIN OUTCOME MEASURES： At 1, 3, and 7 days following intracerebral hemorrhage, superoxide dismutase and malondialdehyde were determined in the brain tissue surrounding the hematoma and in the hippocampus in the affected hemisphere. RESULTS： In brain tissue surrounding the hematoma, superoxide dismutase activity was significantly increased in the bFGF group at 3 and 7 days after intracerebral hemorrhage compared with the saline control group, whereas malondialdehyde content was significantly decreased （P 〈 0.05）. In the hippocampus, superoxide dismutase activity was significantly increased in the bFGF group at 7 days following intracerebral hemorrhage compared with the saline control group, whereas malondialdehyde content was significantly decreased （P 〈 0.05）. At 1, 3, and 7 days after intracerebral hemorrhage, there was no significant difference between the saline control group and the model group with regards to parameter or brain region （P 〉 0.05）. CONCLUSION： Increased superoxide dismutase activity and decreased malondialdehyde content were detected in tissue surrounding the hematoma, as well as the ipsilateral hippocampus, of intracerebral hemorrhage rats treated with bFGF. Changes in these parameters were detected earlier in tissue adjacent to the lesion, compared with the ipsilateral hippocampus.展开更多
Based on the collected multiwavelength data, namely in the radio(NVSS, FIRST, RATAN-600), IR(WISE),optical(Pan-STARRS), UV(GALEX), and X-ray(ROSAT, Swift-XRT) ranges, we have performed a cluster analysis for the blaza...Based on the collected multiwavelength data, namely in the radio(NVSS, FIRST, RATAN-600), IR(WISE),optical(Pan-STARRS), UV(GALEX), and X-ray(ROSAT, Swift-XRT) ranges, we have performed a cluster analysis for the blazars of the Roma-BZCAT catalog. Using two machine learning methods, namely a combination of PCA with k-means clustering and Kohonen's self-organizing maps(SOMs), we have constructed an independent classification of the blazars(five classes) and compared the classes with the known Roma-BZCAT classification(FSRQs, BL Lacs, galaxy-dominated BL Lacs, and blazars of an uncertain type) as well as with the high synchrotron peaked(HSP) blazars from the 3HSP catalog and blazars from the TeVCat catalog. The obtained groups demonstrate concordance with the BL Lac/FSRQ classification along with a continuous character of the change in the properties. The group of HSP blazars stands out against the overall distribution. We examine the characteristics of the five groups and demonstrate distinctions in their spectral energy distribution shapes. The effectiveness of the clustering technique for objective analysis of multiparametric arrays of experimental data is demonstrated.展开更多
Background: Malondialdehyde(MDA) is a candidate general marker of oxidative stress(OS). We sought to assess the relation of MDA to Gulf War illness(GWI) and to a variety of exposures.Methods: This is an observational ...Background: Malondialdehyde(MDA) is a candidate general marker of oxidative stress(OS). We sought to assess the relation of MDA to Gulf War illness(GWI) and to a variety of exposures.Methods: This is an observational study involving subjects from Southern California recruited from October 2011 to May 2014. MDA was assessed in 81 participants(41 GWI-cases, 40 controls). General and Gulf-specific exposures were elicited. MDA case–control comparison was restricted to 40 matched pairs. The potential association between MDA and exposures was assessed using regression analyses. Gulf-specific exposures were incorporated into a case-specific model.Results: Plasma MDA was significantly lower in GWI-cases than controls. Composite pesticide and fuel-solvent exposures negatively predicted MDA in the total sample, as well as in the analyses that included either GWI-cases or controls only. Self-reported exposure to organophosphate(OP) nerve gas was a strong predictor for lower MDA level in veterans with GWI.Conclusions: Past pesticide exposures predicted lower MDA in both veterans with GWI and in healthy controls.展开更多
文摘Oxidative reactions are key part of the stress response in marine organisms, which are exposed to a wide variety of environmental stressors. Lipid peroxidation (LPO) occurs in response to oxidant attack, giving rise to unsaturated aldehydes as malondialdehyde (MDA), the main biomarker for LPO assessment. Levels of MDA are measured in biological samples of different fish matrices to determine the oxidative effect of physical or chemical agents, particularly represented by metals. The most used assay is the spectrophotometric determination of thiobarbituric acid (TBA) as MDA-TBA2 adduct. Selectivity is enhanced by separating the adduct by chromatographic methods such as high-performance liquid chromatography or by using alternative derivatization reagents. Because, in biological organism, MDA is found free or protein bound, the two forms should be measured, and the extraction procedures are a crucial step in the processing of the biological sample. This review focused on MDA determination procedures used to assess the effect of experimental and environmental stress induced in fish.
基金Supported by National Natural Science Foundation of China(31360450)
文摘The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde（ MDA）content. At three-leaf and one-heart stage of Luliangyou 996,15 μg/mL Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. The antioxidant enzyme（ SOD,POD,PPO,CAT and PAL） activities and malondialdehyde（ MDA） content in rice leaves were determined every 24 h within 168 h post spraying. Compared with CK,the MDA content and activities of the five antioxidant enzymes increased in Agantibiotic 702 treatment,Validamycin treatment and M. grisea inoculation treatment. The MDA content and SOD,CAT and POD activities in Ag-antibiotic 702 treatment were significantly higher than those in M. grisea inoculation treatment in early stage,and the APX and LOX activities increased more remarkably and rapidly. The induction effect of Ag-antibiotic 702 could reach or exceed that of Validamycin treatment. Ag-antibiotic 702 enhanced the resistance to rice blast because MDA content and antioxidant enzymes activities were improved in rice leaves. Therefore,the increase of rice blast resistance induced by Ag-antibiotic 702 in rice was positively correlated with the increase of MDA content and antioxidant enzymes activities in rice leaves.
基金Supported by the National Natural Science Foundation of China(Nos.30570404 and 30670458)the Natural Science Foundation of Jilin Province,China(No.20050561)
文摘Effect of Siqi decoction on myocardial ischemia is to prevent cardiac myocyte membrane from damage associated with oxygen free radicals related to NO. To research the regulatoin of the content of malondialdehyde by Siqi decoction, an index of lipid peroxidation, via increasing activity of superoxide dismutase in blood serum of rats with Myocardial Ischemia, the model of myocardium ischemia was made in Wistar rats with posterior pituitary injection through vein in tail. Siqi decoction, Diaoxinxuekang（DK） and Fufangdanshenpian（FD）, the latter two drugs of which are effective TCM drugs of anti-myocardial ischemia at present, were administrated to the rats with myocardium ischemia for 5 days to compare the effect of them on myocardium ischemia as reference drugs via measuring the changes of the content of malondialdehyde and the activity of superoxide dismutase in the rat blood serum with myocardial ischemia. There were a remarkable increase in the activity of superoxide dismutase and a decrease in the content of malondialdehyde in the serum of the rats administered Siqi decoction compared with those of the rats in control group, p〈0.05. The contents of MDA in the serum of the prevention group rats in the experiments are lower than those of the cure group rats. Anti-Myocardium Ischemia mechanism of Siqi decoction is the regulation of the content of malondialdehyde via increasing activity of superoxide dismutase in the serum of Rats with myocardial ischemia and stimulating the activity of NOS in serum so as to increase NO concentration.
文摘Background and objectives: Diabetes is a chronic multifactorial disease which requires a variety of strategies to reduce its epidemic. Type 2 diabetes (T2MD) is the most common form of diabetes which results from inefficiency of insulin secretion or resistance to insulin action, both of which lead to chronic hyperglycemia. Lipid peroxidation is an oxidative stress process that involve in T2DM complications. This study aimed to 1) determine plasma malondialdehyde (MDA) levels as a biomarker for lipid peroxidation in Sudanese patients with T2DM, and 2) assess the associations between MDA and diabetes-related variables. Material and Methods: This case-control study was conducted from November 2022 to June 2023 at the National University, Sudan. It included 100 participants, of whom 50 were T2DM patients and 50 were healthy controls. Data on demographics, clinical characteristics, and biochemical markers (FBS, HbA1c, and MDA) were collected. The NycoCard HbA1c method and the GOD-POD Method were used for HbA1c and glucose measurement, respectively. MDA was determined by the thiobarbituric acid reactive substances method. The data were analyzed using SPSS Version. Results: Significant differences were observed between T2DM patients and healthy controls in FBS (P = 0.000), HbA1c (P = 0.000), and MDA (P = 0.010), with T2DM patients exhibiting higher levels. The study revealed a strong positive correlation between MDA levels and the duration of diabetes (r = 0.69, P = 0.00), while other variables, including age, BMI, and glucose control, did not significantly correlate with MDA levels. Conclusion: Findings revealed elevated MDA levels in Sudanese T2DM patients, with a positive correlation between MDA and diabetes duration. These findings emphasize the importance of oxidative stress in T2DM pathogenesis and call for the need for targeted strategies to mitigate oxidative damage and improve diabetes care.
基金the Science Foundation of Returned Students of Guangxi Zhuang Autonomous Region of China, No. 0575013
文摘BACKGROUND： Studies have confirmed that basic fibroblast growth factor （bFGF） promotes neuronal survival and neurite outgrowth. OBJECTIVE： To compare and verify the effects of bFGF on superoxide dismutase activity and malondialdehyde content in rat brain tissues surrounding a hemorrhagic lesion, as well as the hippocampus at the hemorrhagic side. DESIGN, TIME AND SETTING： The randomized, controlled, neurobiological study was performed at the Science Experimental Center and Research Laboratory, Guangxi Medical University, China, from September to December 2006. MATERIALS： Ninety-two adult, healthy, Wistar rats of equal gender were used to establish intracerebral hemorrhage by infusing type VII collagenase into the left internal capsule. Type Ⅶ collagenase （Sigma, USA）, superoxide dismutase and malondialdehyde kits （Jiancheng, China）, and bFGF （Institute of Bioengineering, Ji＇nan University, China） were used for this study. METHODS： Ninety successfully lesioned rats were equally and randomly divided into three groups. Rats in the bFGF group were intramuscularly injected daily with bFGF （8 ug/kg）. Rats in the saline control group received an equal volume of saline. The rats in the model group did not receive other interventions. Superoxide dismutase activity was measured using the xanthine oxidase method. Malondialdehyde contents were detected using the thiobarbituric acid method. MAIN OUTCOME MEASURES： At 1, 3, and 7 days following intracerebral hemorrhage, superoxide dismutase and malondialdehyde were determined in the brain tissue surrounding the hematoma and in the hippocampus in the affected hemisphere. RESULTS： In brain tissue surrounding the hematoma, superoxide dismutase activity was significantly increased in the bFGF group at 3 and 7 days after intracerebral hemorrhage compared with the saline control group, whereas malondialdehyde content was significantly decreased （P 〈 0.05）. In the hippocampus, superoxide dismutase activity was significantly increased in the bFGF group at 7 days following intracerebral hemorrhage compared with the saline control group, whereas malondialdehyde content was significantly decreased （P 〈 0.05）. At 1, 3, and 7 days after intracerebral hemorrhage, there was no significant difference between the saline control group and the model group with regards to parameter or brain region （P 〉 0.05）. CONCLUSION： Increased superoxide dismutase activity and decreased malondialdehyde content were detected in tissue surrounding the hematoma, as well as the ipsilateral hippocampus, of intracerebral hemorrhage rats treated with bFGF. Changes in these parameters were detected earlier in tissue adjacent to the lesion, compared with the ipsilateral hippocampus.
文摘Based on the collected multiwavelength data, namely in the radio(NVSS, FIRST, RATAN-600), IR(WISE),optical(Pan-STARRS), UV(GALEX), and X-ray(ROSAT, Swift-XRT) ranges, we have performed a cluster analysis for the blazars of the Roma-BZCAT catalog. Using two machine learning methods, namely a combination of PCA with k-means clustering and Kohonen's self-organizing maps(SOMs), we have constructed an independent classification of the blazars(five classes) and compared the classes with the known Roma-BZCAT classification(FSRQs, BL Lacs, galaxy-dominated BL Lacs, and blazars of an uncertain type) as well as with the high synchrotron peaked(HSP) blazars from the 3HSP catalog and blazars from the TeVCat catalog. The obtained groups demonstrate concordance with the BL Lac/FSRQ classification along with a continuous character of the change in the properties. The group of HSP blazars stands out against the overall distribution. We examine the characteristics of the five groups and demonstrate distinctions in their spectral energy distribution shapes. The effectiveness of the clustering technique for objective analysis of multiparametric arrays of experimental data is demonstrated.
基金funded by the Depar tment of Defense Congressionally Directed Medical Research Program (GW093063)。
文摘Background: Malondialdehyde(MDA) is a candidate general marker of oxidative stress(OS). We sought to assess the relation of MDA to Gulf War illness(GWI) and to a variety of exposures.Methods: This is an observational study involving subjects from Southern California recruited from October 2011 to May 2014. MDA was assessed in 81 participants(41 GWI-cases, 40 controls). General and Gulf-specific exposures were elicited. MDA case–control comparison was restricted to 40 matched pairs. The potential association between MDA and exposures was assessed using regression analyses. Gulf-specific exposures were incorporated into a case-specific model.Results: Plasma MDA was significantly lower in GWI-cases than controls. Composite pesticide and fuel-solvent exposures negatively predicted MDA in the total sample, as well as in the analyses that included either GWI-cases or controls only. Self-reported exposure to organophosphate(OP) nerve gas was a strong predictor for lower MDA level in veterans with GWI.Conclusions: Past pesticide exposures predicted lower MDA in both veterans with GWI and in healthy controls.
