[Objectives]This study was conducted to establish an uncertainty evaluation method for the determination of ethyl maltol by ultra-high performance liquid chromatograph-mass spectrometer(UPLC-MS).[Methods]A mathematica...[Objectives]This study was conducted to establish an uncertainty evaluation method for the determination of ethyl maltol by ultra-high performance liquid chromatograph-mass spectrometer(UPLC-MS).[Methods]A mathematical model of uncertainty was established by analyzing the method for determining ethyl maltol using UPLC-MS.The sources of uncertainty were analyzed,and the components of uncertainty were calculated to evaluate the expanded uncertainty of the method.[Results]When the content of ethyl maltol in edible vegetable oil was 1657μg/kg,the expanded uncertainty was 22.4μg/kg(K=2,P=95%).[Conclusions]The uncertainty in this evaluation model mainly came from standard solution preparation,sample weighing,dilution of sample to constant volume,standard curve fitting,and repeated measurement.展开更多
Objective To investigate the impact of sub-chronic Aluminium-maltolate [Al（mal）s] exposure on the catabolism of amyloid precursor protein （APP） in rats. Methods Forty adult male Sprague-Dawley （SD） rats were ran...Objective To investigate the impact of sub-chronic Aluminium-maltolate [Al（mal）s] exposure on the catabolism of amyloid precursor protein （APP） in rats. Methods Forty adult male Sprague-Dawley （SD） rats were randomly divided into five groups： the control group, the maltolate group （7.56 mg/kg BW）, and the Al（mal）s groups （0.27, 0.54, and 1.08 mg/kg BW, respectively）. Control rats were administered with 0.9% normal saline through intraperitoneal （i.p.） injection. Maltolate and Al（mal）s were administered to the rats also through i.p. injections. Administration was conducted daily for two months. Rat neural behavior was examined using open field tests （OFT）. And the protein expressions and their mRNAs transcription related with APP catabolism were studied using enzyme-linked immunosorbent assay （ELISA） and real-time polymerase chain reaction （RT-PCR）. Results The expressions of APP, 13-site APP cleaving enzyme 1 （BACEI） and presenilin-1 （PSi） proteins and their mRNAs transcription increased gradually with the increase of Al（mal）3 doses （P〈0.05）. The enzyme activity of BACEI in the 0.54 and 1.08 mg/kg Al（mal）s groups increased significantly （P〈0.05）. The expression of 8-amyloid protein （AS） 1-40 gradually decreased while the protein expression of A81-42 increased gradually with the increase of Al（mal）s doses （P〈0.05）. Conclusion Result from our study suggested that one of the possible mechanisms that Al（mal）s can cause neurotoxicity is that Al（mal）s can increase the generation of A81-42 by facilitating the expressions of APP, β-, and γ-secretase.展开更多
The deconstructive reorganization strategy for the synthesis of benzene-containing products from the kojic acid-and maltol-derived alkynes has been recently reported.In this strategy,kojic acid and maltol are analogou...The deconstructive reorganization strategy for the synthesis of benzene-containing products from the kojic acid-and maltol-derived alkynes has been recently reported.In this strategy,kojic acid and maltol are analogous to the"Transformers",which can transform into benzofurans and benzaldehydes via annulation reactions.Under the synthetic standpoint,this deconstructive reorganization strategy features high atom economy,innate scalability and functional group tolerance.In the near future,we believe that this unique method will be widely investigated a nd other novel transformations of kojic acid and maltol will be discovered.展开更多
Ferroptosis triggered by hemin is regarded as a primary factor accounting for neuronal death secondary to intracerebral hemorrhage.Thus,compounds with inhibitory effect on hemin-induced ferroptosis might be potential ...Ferroptosis triggered by hemin is regarded as a primary factor accounting for neuronal death secondary to intracerebral hemorrhage.Thus,compounds with inhibitory effect on hemin-induced ferroptosis might be potential medicines to prevent neuronal death caused by intracerebral hemorrhage.Herein,we investigate whether maltol could alleviate hemin-induced SH-SY5Y cell ferroptosis and its potential mechanisms.It is found that maltol effectively prevents hemin-induced SH-SY5Y cell ferroptosis via three pathways.The first one is inhibiting intracellular iron increase via preventing upregulation of transferrin receptor,the second one is alleviating lipid peroxidation via attenuating H_(2)O_(2) generation by NOX4 and promoting H_(2)O_(2) clearance by catalase,and the third one is to reduce peroxidized lipids via maintaining GPX4/GSH pathway.Therefore,maltol is a novel agent preventing hemin-induced SH-SY5Y cell ferroptosis.展开更多
采用蒙德火灾、爆炸及毒性评价方法(imperial chemical industries,ICI)对乙基麦芽酚生产过程中的主要反应单元——氯化工艺单元进行危险性评价,根据物质特性和标准规定,确定了主要可燃物质的物质系数.从单元实际情况出发,计算火灾负荷...采用蒙德火灾、爆炸及毒性评价方法(imperial chemical industries,ICI)对乙基麦芽酚生产过程中的主要反应单元——氯化工艺单元进行危险性评价,根据物质特性和标准规定,确定了主要可燃物质的物质系数.从单元实际情况出发,计算火灾负荷指数、装置内部爆炸指数及单元毒性指数等系数.进一步考虑安全补偿系数,得到乙基麦芽酚氯化单元的全体危险性评分,评价结果为安全管理方式的改进提供理论支撑.展开更多
文摘[Objectives]This study was conducted to establish an uncertainty evaluation method for the determination of ethyl maltol by ultra-high performance liquid chromatograph-mass spectrometer(UPLC-MS).[Methods]A mathematical model of uncertainty was established by analyzing the method for determining ethyl maltol using UPLC-MS.The sources of uncertainty were analyzed,and the components of uncertainty were calculated to evaluate the expanded uncertainty of the method.[Results]When the content of ethyl maltol in edible vegetable oil was 1657μg/kg,the expanded uncertainty was 22.4μg/kg(K=2,P=95%).[Conclusions]The uncertainty in this evaluation model mainly came from standard solution preparation,sample weighing,dilution of sample to constant volume,standard curve fitting,and repeated measurement.
基金supported by the National Natural Science Foundation of China (30972512)the Graduate Innovation Fund of Academic Degree Committee Office of the Shanxi Provincial Government (20093014)+1 种基金Doctor Start-up Fund from Shanxi Medical University (B03201209)the College Students Innovation Fund of Shanxi Medical University (2010-25)
文摘Objective To investigate the impact of sub-chronic Aluminium-maltolate [Al（mal）s] exposure on the catabolism of amyloid precursor protein （APP） in rats. Methods Forty adult male Sprague-Dawley （SD） rats were randomly divided into five groups： the control group, the maltolate group （7.56 mg/kg BW）, and the Al（mal）s groups （0.27, 0.54, and 1.08 mg/kg BW, respectively）. Control rats were administered with 0.9% normal saline through intraperitoneal （i.p.） injection. Maltolate and Al（mal）s were administered to the rats also through i.p. injections. Administration was conducted daily for two months. Rat neural behavior was examined using open field tests （OFT）. And the protein expressions and their mRNAs transcription related with APP catabolism were studied using enzyme-linked immunosorbent assay （ELISA） and real-time polymerase chain reaction （RT-PCR）. Results The expressions of APP, 13-site APP cleaving enzyme 1 （BACEI） and presenilin-1 （PSi） proteins and their mRNAs transcription increased gradually with the increase of Al（mal）3 doses （P〈0.05）. The enzyme activity of BACEI in the 0.54 and 1.08 mg/kg Al（mal）s groups increased significantly （P〈0.05）. The expression of 8-amyloid protein （AS） 1-40 gradually decreased while the protein expression of A81-42 increased gradually with the increase of Al（mal）s doses （P〈0.05）. Conclusion Result from our study suggested that one of the possible mechanisms that Al（mal）s can cause neurotoxicity is that Al（mal）s can increase the generation of A81-42 by facilitating the expressions of APP, β-, and γ-secretase.
基金the National Natural Science Foundation of China(Nos.21871053 and 21532001)the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(No.2019R01005)。
文摘The deconstructive reorganization strategy for the synthesis of benzene-containing products from the kojic acid-and maltol-derived alkynes has been recently reported.In this strategy,kojic acid and maltol are analogous to the"Transformers",which can transform into benzofurans and benzaldehydes via annulation reactions.Under the synthetic standpoint,this deconstructive reorganization strategy features high atom economy,innate scalability and functional group tolerance.In the near future,we believe that this unique method will be widely investigated a nd other novel transformations of kojic acid and maltol will be discovered.
基金supported by the National Natural Science Foundation of China(Nos.31900742,81701293,81972346)the Scientific Research Foundation of Jilin province,China(Nos.20190701051GH,20200201405JC)the Achievement Transformation Fund of the First Hospital of Jilin University,China(No.CGZHYD202012-028).
文摘Ferroptosis triggered by hemin is regarded as a primary factor accounting for neuronal death secondary to intracerebral hemorrhage.Thus,compounds with inhibitory effect on hemin-induced ferroptosis might be potential medicines to prevent neuronal death caused by intracerebral hemorrhage.Herein,we investigate whether maltol could alleviate hemin-induced SH-SY5Y cell ferroptosis and its potential mechanisms.It is found that maltol effectively prevents hemin-induced SH-SY5Y cell ferroptosis via three pathways.The first one is inhibiting intracellular iron increase via preventing upregulation of transferrin receptor,the second one is alleviating lipid peroxidation via attenuating H_(2)O_(2) generation by NOX4 and promoting H_(2)O_(2) clearance by catalase,and the third one is to reduce peroxidized lipids via maintaining GPX4/GSH pathway.Therefore,maltol is a novel agent preventing hemin-induced SH-SY5Y cell ferroptosis.
文摘采用蒙德火灾、爆炸及毒性评价方法(imperial chemical industries,ICI)对乙基麦芽酚生产过程中的主要反应单元——氯化工艺单元进行危险性评价,根据物质特性和标准规定,确定了主要可燃物质的物质系数.从单元实际情况出发,计算火灾负荷指数、装置内部爆炸指数及单元毒性指数等系数.进一步考虑安全补偿系数,得到乙基麦芽酚氯化单元的全体危险性评分,评价结果为安全管理方式的改进提供理论支撑.
