Four short-term in vivo and in vitro tests were used to further confirm the antitumor activities of MCP, a vegetable powder, prepared from Malru crispo L. (i) In the H22 hepatoma-transplanting test, MCP had antitumor ...Four short-term in vivo and in vitro tests were used to further confirm the antitumor activities of MCP, a vegetable powder, prepared from Malru crispo L. (i) In the H22 hepatoma-transplanting test, MCP had antitumor action, but MCP residue did not show such action; 5-FU appeared to have more potent antitumor activities and more harmful effects than MCP. (ii) In the micronucleus (MN) test, MCP significantly decreased MN frequency. (iii) In the cancer cell culture systems, the MCP fat-soluble extract revealed inhibitory effects on the growth and proliferation of the human hepatoma and the gastric cancer cells in a dose-response manner. (iv) In the colony formation test, MCP also altered the morphology of human gastric cancer cells. It was suggested that MCP could be consumed not only by healthy subJects for cancer prevention but also by patients with cancer as supplernentary treatrnent in combination with anticarcinogenic drug such as 5-FU, cyclophosphamide (CP)展开更多
The acute toxicity of Sodium dodecylbenzene sulfonate （SDBS） to subcamersed macrophyte Hydrilla verticillata （L.f） Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes： superoxide d...The acute toxicity of Sodium dodecylbenzene sulfonate （SDBS） to subcamersed macrophyte Hydrilla verticillata （L.f） Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes： superoxide dismutase （SOD, EC 1.15.1.1）, catalase （CAT, EC 1.11.1.6）, peroxidase （POD, EC 1.11.1.7） in the leaves were investigated under different concentrations of SDBS, The chlorophyll contents in leaves of experimental plants decreased gradually, while the electrical conductivity of culture solution increased gradually with the increased dose of SDBS. Under higher concentrations of SDBS （32 mg/L and 128 mg/L） treatment, SOD lost its activities completely after 24 h. The native PAGE analysis indicated the diversity of POD isoenzymes was obvious under different concentrations of SDBS stress.展开更多
Fatty, phenolic and hydroxy acids in aqueous leachates of three submerged macrophytes 〔Ceratophyllum demersum L., Vallisneria spiralis L., Hydrilla verticillata (L. f.) Royle〕 were analyzed using GC-MS in shallow fr...Fatty, phenolic and hydroxy acids in aqueous leachates of three submerged macrophytes 〔Ceratophyllum demersum L., Vallisneria spiralis L., Hydrilla verticillata (L. f.) Royle〕 were analyzed using GC-MS in shallow freshwater. Succinic acid, oxalic acid, cis-aconitic acid, lactic acid and ferulic acid were main components. The content of cis-aconitic acid was more than 18% in H. verticillata but less than 2% in C. demersum and V. spiralis. The content of phenolic acid accounted for 8.85% in C. demersum but 1.23% and 0.63% in V. spiralis and H. verticillata.展开更多
文摘Four short-term in vivo and in vitro tests were used to further confirm the antitumor activities of MCP, a vegetable powder, prepared from Malru crispo L. (i) In the H22 hepatoma-transplanting test, MCP had antitumor action, but MCP residue did not show such action; 5-FU appeared to have more potent antitumor activities and more harmful effects than MCP. (ii) In the micronucleus (MN) test, MCP significantly decreased MN frequency. (iii) In the cancer cell culture systems, the MCP fat-soluble extract revealed inhibitory effects on the growth and proliferation of the human hepatoma and the gastric cancer cells in a dose-response manner. (iv) In the colony formation test, MCP also altered the morphology of human gastric cancer cells. It was suggested that MCP could be consumed not only by healthy subJects for cancer prevention but also by patients with cancer as supplernentary treatrnent in combination with anticarcinogenic drug such as 5-FU, cyclophosphamide (CP)
基金the National Natural Science Foundation of China (39925007)the National High Technology Research and Development Program of China (863 Program) (2002AA601021)the Knowledge Innovation Program Key Project of Chinese Academy Sciences (KSCX2-SW-102)
文摘The acute toxicity of Sodium dodecylbenzene sulfonate （SDBS） to subcamersed macrophyte Hydrilla verticillata （L.f） Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes： superoxide dismutase （SOD, EC 1.15.1.1）, catalase （CAT, EC 1.11.1.6）, peroxidase （POD, EC 1.11.1.7） in the leaves were investigated under different concentrations of SDBS, The chlorophyll contents in leaves of experimental plants decreased gradually, while the electrical conductivity of culture solution increased gradually with the increased dose of SDBS. Under higher concentrations of SDBS （32 mg/L and 128 mg/L） treatment, SOD lost its activities completely after 24 h. The native PAGE analysis indicated the diversity of POD isoenzymes was obvious under different concentrations of SDBS stress.
文摘Fatty, phenolic and hydroxy acids in aqueous leachates of three submerged macrophytes 〔Ceratophyllum demersum L., Vallisneria spiralis L., Hydrilla verticillata (L. f.) Royle〕 were analyzed using GC-MS in shallow freshwater. Succinic acid, oxalic acid, cis-aconitic acid, lactic acid and ferulic acid were main components. The content of cis-aconitic acid was more than 18% in H. verticillata but less than 2% in C. demersum and V. spiralis. The content of phenolic acid accounted for 8.85% in C. demersum but 1.23% and 0.63% in V. spiralis and H. verticillata.
