Manganese peroxidases(MnP)from Phanerochaete chrysosporium were adsorbed onto multi-walled carbon nanotubes(MWNT).Four different loadings of MnP on MWNTs were investigated,and the maximum enzyme loading of 47.5µg...Manganese peroxidases(MnP)from Phanerochaete chrysosporium were adsorbed onto multi-walled carbon nanotubes(MWNT).Four different loadings of MnP on MWNTs were investigated,and the maximum enzyme loading of 47.5µg/mg of MWNTs was obtained in 12 h.The adsorbed MnP showed a catalytic activity of up to 0.1 U/mg of the weight of the system of MnP/MWNTs,with 23%of its original activity retained.The AFM image of the adsorbed enzymes indicated that a layer of MnP covered the surface of the MWNTs and retained its original three-dimensional shape.Amino-based nonspecific interactions may play the dominant role in the adsorption of MnP on MWNTs.展开更多
White rot fungus Pleurotus djamor H1 was stilly cultured at 28 ℃ under 4 different kinds of LNAS culture solution,the extracellular enzyme solutions were sampled at different interval,O.D.values of the solutions,repr...White rot fungus Pleurotus djamor H1 was stilly cultured at 28 ℃ under 4 different kinds of LNAS culture solution,the extracellular enzyme solutions were sampled at different interval,O.D.values of the solutions,representing manganese peroxidase(MnP),laccase and lignin peroxidase(LiP)activities,were measured spectrophotometrically by monitoring the oxidation of 2,6-DMP at 470 nm,ABTS at 420 nm and veratryl alcohol(VA)at 310 nm,the lignin-degrading enzymes of the fungus and the relationships between enzyme activities and medium composition and substrates were gained.Results indicated that Pleurotus djamor could produce MnP and laccase simultaneously,but no Lip.Substrates wood sawdust and 2,6-DMP could significantly enhanced MnP and laccase activities,the biggest Mnp and laccase activity were 5 U·L-1.and 38 U·L-1 when no substrates were added,and 69 U·L-1 and 205 U·L-1 when substrates added.The paper offered a basic ligninolytic enzymology of Pleurotus djamor for future utilization of the enzymes and further study on MnP and laccase mechanism of the fungus.展开更多
基金the National Natural Science Foundation of China(Grant No.20677033)the special fund of State Key Joint Laboratory of Environment Simulation and Pollution Control,China(08Z01ESPCT).
文摘Manganese peroxidases(MnP)from Phanerochaete chrysosporium were adsorbed onto multi-walled carbon nanotubes(MWNT).Four different loadings of MnP on MWNTs were investigated,and the maximum enzyme loading of 47.5µg/mg of MWNTs was obtained in 12 h.The adsorbed MnP showed a catalytic activity of up to 0.1 U/mg of the weight of the system of MnP/MWNTs,with 23%of its original activity retained.The AFM image of the adsorbed enzymes indicated that a layer of MnP covered the surface of the MWNTs and retained its original three-dimensional shape.Amino-based nonspecific interactions may play the dominant role in the adsorption of MnP on MWNTs.
文摘White rot fungus Pleurotus djamor H1 was stilly cultured at 28 ℃ under 4 different kinds of LNAS culture solution,the extracellular enzyme solutions were sampled at different interval,O.D.values of the solutions,representing manganese peroxidase(MnP),laccase and lignin peroxidase(LiP)activities,were measured spectrophotometrically by monitoring the oxidation of 2,6-DMP at 470 nm,ABTS at 420 nm and veratryl alcohol(VA)at 310 nm,the lignin-degrading enzymes of the fungus and the relationships between enzyme activities and medium composition and substrates were gained.Results indicated that Pleurotus djamor could produce MnP and laccase simultaneously,but no Lip.Substrates wood sawdust and 2,6-DMP could significantly enhanced MnP and laccase activities,the biggest Mnp and laccase activity were 5 U·L-1.and 38 U·L-1 when no substrates were added,and 69 U·L-1 and 205 U·L-1 when substrates added.The paper offered a basic ligninolytic enzymology of Pleurotus djamor for future utilization of the enzymes and further study on MnP and laccase mechanism of the fungus.
