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磁共振T2-mapping评估Graves眼病活动性的价值
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作者 王璐 樊瑶 +2 位作者 龙健 张明巧 刘纯 《解放军医学杂志》 CAS CSCD 北大核心 2024年第1期70-74,共5页
目的探讨磁共振T_(2)-mapping在Graves眼病(GO)活动性评估方面的价值。方法收集2019年7月-2021年1月于重庆医科大学附属第一医院内分泌科就诊的GO患者64例,使用计算机对其进行简单随机分组,其中49例作为观察研究对象,另外15例进行诊断... 目的探讨磁共振T_(2)-mapping在Graves眼病(GO)活动性评估方面的价值。方法收集2019年7月-2021年1月于重庆医科大学附属第一医院内分泌科就诊的GO患者64例,使用计算机对其进行简单随机分组,其中49例作为观察研究对象,另外15例进行诊断试验评价。按照临床活动性评分(CAS)将49例GO患者分为活动组(CAS≥3分,48只患眼)与非活动组(CAS<3分,50只患眼)。同期招募31名健康志愿者作为正常对照组,共62只眼。所有人均行3.0T眼眶磁共振T_(2)-mapping成像。在T_(2)-mapping冠状位图像上测量上直肌、下直肌、内直肌、外直肌眼球后5个层面的T_(2)弛豫时间(T_(2)RT),每块眼外肌分别取眼球后5个层面的T_(2)RT最大值来代表该眼外肌的T_(2)RT,最后取4块眼外肌T_(2)RT中的最大值,以眼外肌最大T_(2)RT表示。比较活动组、非活动组与正常对照组间上述5个指标(上直肌T_(2)RT、下直肌T_(2)RT、内直肌T_(2)RT、外直肌T_(2)RT、眼外肌最大T_(2)RT)的差异;采用受试者工作特征(ROC)曲线分析上述5个指标对GO活动性评估的诊断价值,得出诊断阈值,然后利用另外15例GO患者进行诊断试验评价,确定诊断效能较高的指标及其诊断活动性的阈值。结果活动组所有眼外肌的T_(2)RT均明显高于非活动组及正常对照组,差异有统计学意义(P<0.001)。ROC曲线分析显示,上直肌、下直肌、内直肌、外直肌、眼外肌的最大T_(2)RT判断活动性的截断值分别为80.200 ms、97.045 ms、94.355 ms、85.750 ms、101.385 ms。利用另外15例GO患者进行诊断试验评价,综合评估敏感度、特异度、阳性预测值、阴性预测值较高的指标为下直肌T_(2)RT、眼外肌最大T_(2)RT,其判断活动性的截断值分别为97.045 ms、101.385 ms;敏感度分别为91.7%、93.8%;特异度均为80.0%。结论磁共振T_(2)-mapping序列对评估GO的活动性具有良好价值。临床上可选择测量下直肌T_(2)RT或眼外肌最大T_(2)RT协助评估GO的活动性。 展开更多
关键词 GRAVES眼病 磁共振T2-mapping 活动性评估
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Visualization for Explanation of Deep Learning-Based Defect Detection Model Using Class Activation Map 被引量:1
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作者 Hyunkyu Shin Yonghan Ahn +3 位作者 Mihwa Song Heungbae Gil Jungsik Choi Sanghyo Lee 《Computers, Materials & Continua》 SCIE EI 2023年第6期4753-4766,共14页
Recently,convolutional neural network(CNN)-based visual inspec-tion has been developed to detect defects on building surfaces automatically.The CNN model demonstrates remarkable accuracy in image data analysis;however... Recently,convolutional neural network(CNN)-based visual inspec-tion has been developed to detect defects on building surfaces automatically.The CNN model demonstrates remarkable accuracy in image data analysis;however,the predicted results have uncertainty in providing accurate informa-tion to users because of the“black box”problem in the deep learning model.Therefore,this study proposes a visual explanation method to overcome the uncertainty limitation of CNN-based defect identification.The visual repre-sentative gradient-weights class activation mapping(Grad-CAM)method is adopted to provide visually explainable information.A visualizing evaluation index is proposed to quantitatively analyze visual representations;this index reflects a rough estimate of the concordance rate between the visualized heat map and intended defects.In addition,an ablation study,adopting three-branch combinations with the VGG16,is implemented to identify perfor-mance variations by visualizing predicted results.Experiments reveal that the proposed model,combined with hybrid pooling,batch normalization,and multi-attention modules,achieves the best performance with an accuracy of 97.77%,corresponding to an improvement of 2.49%compared with the baseline model.Consequently,this study demonstrates that reliable results from an automatic defect classification model can be provided to an inspector through the visual representation of the predicted results using CNN models. 展开更多
关键词 Defect detection VISUALIZATION class activation map deep learning EXPLANATION visualizing evaluation index
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Effects of Co_3O_4 nanocatalyst morphology on CO oxidation:Synthesis process map and catalytic activity 被引量:6
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作者 曾良鹏 李孔斋 +2 位作者 黄樊 祝星 李宏程 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2016年第6期908-922,共15页
This study focuses on drawing a hydrothermal synthesis process map for Co3O4 nanoparticles with various morphologies and investigating the effects of Co3O4 nanocatalyst morphology on CO oxidation.A series of cobalt-hy... This study focuses on drawing a hydrothermal synthesis process map for Co3O4 nanoparticles with various morphologies and investigating the effects of Co3O4 nanocatalyst morphology on CO oxidation.A series of cobalt-hydroxide-carbonate nanoparticles with various morphologies(i.e.,nanorods,nanosheets,and nanocubes) were successfully synthesized,and Co3O4 nanoparticles were obtained by thermal decomposition of the cobalt-hydroxide-carbonate precursors.The results suggest that the cobalt source is a key factor for controlling the morphology of cobalt-hydroxide-carbonate at relatively low hydrothermal temperatures(≤ 140℃).Nanorods can be synthesized in CoCl2 solution,while Co(NO3)2 solution promotes the formation of nanosheets.Further increasing the synthesis temperature(higher than 140 ℃) results in the formation of nanocubes in either Co(NO3)2 or CoCl2 solution.The reaction time only affects the size of the obtained nanoparticles.The presence of CTAB could improve the uniformity and dispersion of particles.Co3O4 nanosheets showed much higher catalytic activity for CO oxidation than nanorods and nanocubes because it has more abundant Co^(3+) on the surface,much higher reducibility,and better oxygen desorption capacity. 展开更多
关键词 Cobalt oxide nanocatalyst Synthesis process map Morphology effect Catalytic activity Carbon monoxide oxidation
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Value Analysis among the Parties on Imported Product in Indonesia Using Process Activity Mapping: Freight Forwarder, Distributor, and Retailer
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作者 Megita Ryanjani Tanuputri Ravipim Chaveesuk Adi Djoko Guritno 《Journal of Food Science and Engineering》 2017年第5期274-282,共9页
National logistics system in Indonesia can be categorized as inefficient logistics system due to current number of non-value added (NVA) activities. The unreliable National logistics system and the complexity of dis... National logistics system in Indonesia can be categorized as inefficient logistics system due to current number of non-value added (NVA) activities. The unreliable National logistics system and the complexity of distribution system are the big obstacles. This study aims to analyze the value and to propose recommendation for further improvement of National distribution system for imported product. This study employed convenience sampling through in-depth interview to analyze the activity in freight forwarder (FF), distributor and retailer. To demonstrate and analyze the activity process in each party, Process Activity Mapping (PAM) was used as a tool. The study results showed that the delivery speed in Jakarta (8.4 min/km) is slower than that of Surabaya (6.6 min/km). The government support through creating adequate infrastructure, good bureaucracy system and good collaboration directly affects the activities of FF, distributor and retailer. Improving FF performance in a timely and reliable manner is required to reduce errors that may occur. Moreover, encouraging the practice of cold chain management is also necessary in distributing the product throughout Indonesia. The pull strategy can be chosen by retailer to eliminate storage activity. Meanwhile, the use of information technology (IT) system is essential to encourage better inventory management, database management and sharing information in distributor and retailer stage. 展开更多
关键词 Value analysis import Indonesia logistics system process activity mapping.
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Comparison of detection results of hypoxic-ischemic encephalopathy at different degrees in infant patients between brain electrical activity mapping, transcranial Doppler sonography and computer tomography examinations
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作者 Dongruo He Xiaoying Xu +1 位作者 Yinghui Zhang Guochao Han 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第4期379-381,共3页
BACKGROUND: It has been proved that brain electrical activity mapping (BEAM) and transcranial Doppler (TCD) detection can reflect the function of brain cell and its diseased degree of infant patients with moderat... BACKGROUND: It has been proved that brain electrical activity mapping (BEAM) and transcranial Doppler (TCD) detection can reflect the function of brain cell and its diseased degree of infant patients with moderate to severe hypoxic-ischemic encephalopathy (HIE). OBJECTIVE: To observe the abnormal results of HIE at different degrees detected with BEAM and TCD in infant patients, and compare the detection results at the same time point between BEAM, TCD and computer tomography (CT) examinations. DESIGN : Contrast observation SETTING: Departments of Neuro-electrophysiology and Pediatrics, Second Affiliated Hospital of Qiqihar Medical College. PARTICIPANTS: Totally 416 infant patients with HIE who received treatment in the Department of Newborn Infants, Second Affiliated Hospital of Qiqihar Medical College during January 2001 and December 2005. The infant patients, 278 male and 138 female, were at embryonic 37 to 42 weeks and weighing 2.0 to 4.1 kg, and they were diagnosed with CT and met the diagnostic criteria of HIE of newborn infants compiled by Department of Neonatology, Pediatric Academy, Chinese Medical Association. According to diagnostic criteria, 130 patients were mild abnormal, 196 moderate abnormal and 90 severe abnormal. The relatives of all the infant patients were informed of the experiment. METHOOS: BEAM and TCD examinations were performed in the involved 416 infant patients with HIE at different degrees with DYD2000 16-channel BEAM instrument and EME-2000 ultrasonograph before preliminary diagnosis treatment (within 1 month after birth) and 1,3,6,12 and 24 months after birth, and detected results were compared between BEAM, TCD and CT examinations. MAIN OUTCOME MEASURES: Comparison of detection results of HIE at different time points in infant patients between BEAM. TCD and CT examinations. RESULTS: All the 416 infant patients with HIE participated in the result analysis. (1) Comparison of the detected results in infant patients with mild HIE at different time points after birth between BEAM, TCD and CT examinations: BEAM examination showed that the recovery was delayed, and the abnormal rate of BEAM examination was significantly higher than that of CT examination 1 and 3 months after birth [55.4%(72/130)vs. 17.0% (22/130 ),x^2=41.66 ;29.2% ( 38/130 ) vs. 6.2% ( 8/130 ), x^2=23.77, P 〈 0.01 ], exceptional patients had mild abnormality and reached the normal level in about 6 months. TCD examination showed that the disease condition significantly improved and infant patients with HIE basically recovered 1 or 2 months after birth, while CT examination showed that infant patients recovered 3 or 4 months after birth. (2) Comparison of detection results of infant patients with moderate HIE at different time points between BEAM, TCD and CT examinations: The abnormal rate of BEAM examination was significantly higher than that of CT examination 1,3,6 and 12 months after birth [90.8% (178/196),78.6% (154/196),x^2=4.32,P 〈 0.05;64.3% (126/196),43.9% (86/196) ,x^2=16.44 ;44.9% (88/196) ,22.4% (44/196),x^2=22.11 ;21.4% (42/196), 10.2% (20/196),x^2=9.27, P 〈 0.01]. BEAM examination showed that there was still one patient who did not completely recovered in the 24^th month due to the relatives of infant patients did not combine the treatment,. TCD examination showed that the abnormal rate was 23.1%(30/196)in the 1^st month after birth, and all the patients recovered to the normal in the 3^rd month after birth, while CT examination showed that mild abnormality still existed in the 24^th month after birth (1.0% ,2/196). (3) Comparison of detection results of infant patients with severe HIE at different time points between BEAM, TCD and CT examinations: The abnormal rate of BEAM examination was significantly higher than that of CT examination in the 1^st, 3^rd, 6^th and 12^th months after birth[86.7% (78/90),44.4% (40/90),x^2=35.53;62.2% (56/90),31.1% (28/90),x^2=17.51 ;37.8% (34/90),6.7% (6/90), x^2=27.14, P 〈 0.01]. BEAM examination showed that mild abnormality still existed in 4 infant patients in the 24^th month after birth. TCD examination showed that the abnormal rate was 11.1% (10/90) in the 3^rd month after birth, and all the infant patients recovered in the 6^th month after birth. CT examination showed that the abnormal rate was 6.7%(6/90) in the 12^th month after birth, and all of infant patients recovered to the normal in the 24^th month after birth.CONCLUSION : BEAM is the direct index to detect brain function of infant patients with HIE, and positive reaction is still very sensitive in the tracking detection of convalescent period. The positive rate of morphological reaction in CT examination is superior to that in TCD examination, and the positive rate is very high in the acute period of HIE in examination. 展开更多
关键词 HIE Comparison of detection results of hypoxic-ischemic encephalopathy at different degrees in infant patients between brain electrical activity mapping transcranial Doppler sonography and computer tomography examinations
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Oracle Spatial & MapViewer在汽车导航定位上的应用 被引量:1
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作者 董元 张时忠 《计算机工程与科学》 CSCD 2008年第6期119-120,151,共3页
本文通过Oracle数据库的空间数据管理模块Oracle Spatial & MapViewer,将汽车导航定位的实时数据直接写入空间数据库,再用MapViewer发送回终端进行显示;提出了一种用Oracle Spatial & MapViewer对实时定位数据进行存储显示和共... 本文通过Oracle数据库的空间数据管理模块Oracle Spatial & MapViewer,将汽车导航定位的实时数据直接写入空间数据库,再用MapViewer发送回终端进行显示;提出了一种用Oracle Spatial & MapViewer对实时定位数据进行存储显示和共享的新方法。 展开更多
关键词 空间数据库 汽车定位 ORACLE SPATIAL map viewe.
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基于Photo Sphere Viewer的轻量化可量测街景系统研究
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作者 刘一宁 王琳 岳照溪 《测绘通报》 CSCD 北大核心 2024年第S01期11-17,共7页
随着新型基础测绘体系发展,测绘技术和装备不断升级,大量街景数据随着车载激光扫描工作一并被采集。针对该数据路线随机性大、密集程度高等特点,以及传统街景地图系统插件受限、扩展性不强、无法量测等局限,本文设计了基于Photo Sphere ... 随着新型基础测绘体系发展,测绘技术和装备不断升级,大量街景数据随着车载激光扫描工作一并被采集。针对该数据路线随机性大、密集程度高等特点,以及传统街景地图系统插件受限、扩展性不强、无法量测等局限,本文设计了基于Photo Sphere Viewer的轻量化可量测街景系统,在开源插件的基础上实现了对其组件的扩充、实现了行进方向、地图交互等系统功能的设计优化,进一步实现了基于深度图的街景地图量测功能。生产作业队伍对系统的使用结果表明,该系统可以辅助生产作业队伍进行属性采集、纹理更新、数据检查等多项工作,操作灵活便捷,且具有较强的可扩展性,便于基于生产需求进行改进调整,提高了生产效率,为新型基础测绘成果进一步深化应用提供了良好的借鉴作用。 展开更多
关键词 Photo Sphere viewer 轻量化 街景量测 街景数据组织 新型基础测绘
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Triptolide (PG-490) induces apoptosis of dendritic cells through sequential p38 MAP kinase phosphorylation and caspase 3 activation 被引量:41
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作者 LiuQ ChenT ChenH ZhangM LiN LuZ MaP CaoX 《第二军医大学学报》 CAS CSCD 北大核心 2004年第9期939-939,共1页
Dendritic cells (DCs) are the most potent antigen-presen ting cells that play crucial roles in the regulation of immune response. Triptol ide, an active component purified from the medicinal plant Tripterygium wilfor ... Dendritic cells (DCs) are the most potent antigen-presen ting cells that play crucial roles in the regulation of immune response. Triptol ide, an active component purified from the medicinal plant Tripterygium wilfor dii Hook F., has been demonstrated to act as a potent immunosuppressive drug c apab le of inhibiting T cell activation and proliferation. However, little is known a bout the effects of triptolide on DCs. The present study shows that triptolide d oes not affect phenotypic differentiation and LPS-induced maturation of murine DCs. But triptolide can dramatically reduce cell recovery by inducing apoptosis of DCs at concentration as low as 10 ng/ml, as demonstrated by phosphatidylserin e exposure, mitochondria potential decrease, and nuclear DNA condensation. Tript olide induces activation of p38 in DCs, which precedes the activation of caspase 3. SB203580, a specific kinase inhibitor for p38, can block the activation of caspase 3 and inhibit the resultant apoptosis of DCs. Our results suggest that t he anti-inflammatory and immunosuppressive activities of triptolide may be due, in part, to its apoptosis-inducing effects on DCs. 展开更多
关键词 PG-490 map kinase phosphorylation and caspase 3 activation TRIPTOLIDE
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基于MAP法和HAP法的剩余污泥中磷回收技术研究进展
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作者 滕俊 罗进财 +1 位作者 庞福静 董姗燕 《四川环境》 2023年第4期328-337,共10页
剩余污泥中磷的回收利用是实现污水和剩余污泥中磷元素循环利用的有效途径。综述了磷酸铵镁结晶法(MAP法)和磷酸钙结晶法(HAP法)两种磷回收技术,指出两种方法均是有效的磷回收技术,二者反应条件对磷回收的影响具有一定的相似性,不同之... 剩余污泥中磷的回收利用是实现污水和剩余污泥中磷元素循环利用的有效途径。综述了磷酸铵镁结晶法(MAP法)和磷酸钙结晶法(HAP法)两种磷回收技术,指出两种方法均是有效的磷回收技术,二者反应条件对磷回收的影响具有一定的相似性,不同之处在于MAP法可以同时将污水中的氨氮和磷酸盐回收去除,而HAP法适用于含磷废水中磷的去除回收;阐述了晶种是促进产物生成效率和提高产物纯度的有效方法,而污泥中有机物、相关离子或重金属等干扰因素则对产物生成效率产生一定影响;最后提出污泥厌氧发酵过程中有机物及其转化与磷释放和磷回收机制的影响将是今后的研究重点,以实现磷和有机酸等物质的同步回收利用。 展开更多
关键词 剩余污泥 磷回收 map HAP法 晶种
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An Activity Recognition System at Home Based on CO2 Sensors
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作者 Hiroyuki Matsubara 《Journal of Computer and Communications》 2023年第11期64-77,共14页
Activity recognition of indoor occupants using indirect sensing with less privacy violation is one of the hot research topics. This paper proposes a CO<sub>2</sub> sensor-based indoor occupant activity mon... Activity recognition of indoor occupants using indirect sensing with less privacy violation is one of the hot research topics. This paper proposes a CO<sub>2</sub> sensor-based indoor occupant activity monitoring system. Using the IoT sensor node that contains CO<sub>2</sub> sensors, the measured CO<sub>2</sub> concentrations in three locations (laboratory, office, and bedroom) were stored in a cloud server for up to 35 days starting July 1, 2023. The CO<sub>2</sub> measurements stored at 30-second intervals were statistically processed to produce a heat-mapped display of the hourly average or maximum CO<sub>2</sub> concentration. From the heatmap visualizations of CO<sub>2</sub> concentration, the proposed system estimated meeting, heating water using a portable stove, and sleep for the occupants’ activity recognition. 展开更多
关键词 activity Recognition CO2 Sensor Internet of Things (IoT) Low Privacy-Intrusion Heat map
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Study Progress Analysis of Effluent Quality Prediction in Activated Sludge Process Based on CiteSpace
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作者 Kemeng Xue 《Journal of Water Resource and Protection》 CAS 2024年第6期450-465,共16页
In this paper, CiteSpace, a bibliometrics software, was adopted to collect research papers published on the Web of Science, which are relevant to biological model and effluent quality prediction in activated sludge pr... In this paper, CiteSpace, a bibliometrics software, was adopted to collect research papers published on the Web of Science, which are relevant to biological model and effluent quality prediction in activated sludge process in the wastewater treatment. By the way of trend map, keyword knowledge map, and co-cited knowledge map, specific visualization analysis and identification of the authors, institutions and regions were concluded. Furthermore, the topics and hotspots of water quality prediction in activated sludge process through the literature-co-citation-based cluster analysis and literature citation burst analysis were also determined, which not only reflected the historical evolution progress to a certain extent, but also provided the direction and insight of the knowledge structure of water quality prediction and activated sludge process for future research. 展开更多
关键词 Biological Model Effluent Quality Prediction activated Sludge Process CITESPACE Knowledge map Co-Citation Cluster Analysis
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3DV quality model based depth maps for view synthesis in FTV system
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作者 张秋闻 安平 +2 位作者 张艳 张兆杨 王元庆 《Journal of Shanghai University(English Edition)》 CAS 2011年第4期335-341,共7页
Depth maps are used for synthesis virtual view in free-viewpoint television (FTV) systems. When depth maps are derived using existing depth estimation methods, the depth distortions will cause undesirable artifacts ... Depth maps are used for synthesis virtual view in free-viewpoint television (FTV) systems. When depth maps are derived using existing depth estimation methods, the depth distortions will cause undesirable artifacts in the synthesized views. To solve this problem, a 3D video quality model base depth maps (D-3DV) for virtual view synthesis and depth map coding in the FTV applications is proposed. First, the relationships between distortions in coded depth map and rendered view are derived. Then, a precisely 3DV quality model based depth characteristics is develop for the synthesized virtual views. Finally, based on D-3DV model, a multilateral filtering is applied as a pre-processed filter to reduce rendering artifacts. The experimental results evaluated by objective and subjective methods indicate that the proposed D-3DV model can reduce bit-rate of depth coding and achieve better rendering quality. 展开更多
关键词 free-viewpoint television (FTV) 3D video quality model base depth maps (D-3DV) view synthesis
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Active Mapping防止NIDS误报和漏报的重要手段
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作者 都林 叶小列 《计算机安全》 2004年第9期47-48,共2页
关键词 网络入侵检测系统 NIDS TCP/IP协议 activE mapPING
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Hot compression behavior and processing map of cast Mg-4Al-2Sn-Y-Nd alloy 被引量:5
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作者 王晶 史宝良 杨院生 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2014年第3期626-631,共6页
The plastic flow behavior of a newly developed high ductility magnesium alloy, Mg-4Al-2Sn-Y-Nd, was investigated by hot compression from 200 to 400 ℃ with a strain rate of 1.5×10-3 to 7.5 s-1. The results reveal... The plastic flow behavior of a newly developed high ductility magnesium alloy, Mg-4Al-2Sn-Y-Nd, was investigated by hot compression from 200 to 400 ℃ with a strain rate of 1.5×10-3 to 7.5 s-1. The results reveal that the strain rate sensitivity factor (m) of the alloy is much lower than that of the AZ31 alloy, which implies that the alloy should be more suitable for processing at high strain rate. The constitutive relationship of the alloy deformed at elevated temperature was obtained by plotting the experimental data. The stress exponent of the alloy is 10.33, which reveals that climb-controlled dislocations creep is the dominated deformation mechanism. The processing-map technique was used to determine the practical processing window. The proper deformation temperature and strain rate of the cast alloy were determined as 350-400 ℃ and 0.01-0.03 s-1, respectively. 展开更多
关键词 magnesium alloy uniaxial compression apparent activation energy processing map
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MAPK信号通路在肝癌发生发展及治疗中的作用 被引量:18
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作者 次旦旺久 林坤 +2 位作者 卢再鸣 赵相轩 王晓明 《临床肝胆病杂志》 CAS 2016年第9期1810-1813,共4页
丝裂原活化蛋白激酶(MAPK)信号通路的异常活化与肝癌的发生、发展、转移密切相关。介绍了MAPK通路蛋白在肝癌中的表达及其在肝癌增殖、分化、转移中的作用,阐述了MAPK信号通路在肝癌治疗及预后评价中的价值。认为MAPK信号通路在肝癌的... 丝裂原活化蛋白激酶(MAPK)信号通路的异常活化与肝癌的发生、发展、转移密切相关。介绍了MAPK通路蛋白在肝癌中的表达及其在肝癌增殖、分化、转移中的作用,阐述了MAPK信号通路在肝癌治疗及预后评价中的价值。认为MAPK信号通路在肝癌的发生发展及治疗中发挥非常重要的作用,是肝癌治疗及预后评价的潜在分子靶点。 展开更多
关键词 肝肿瘤 丝裂原激活蛋白激酶类 map激酶信号系统 综述
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代谢综合征大鼠肥胖特征及ERK1/2和p38MAPK的作用研究 被引量:9
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作者 张雅萍 祝之明 +5 位作者 闫振成 钟健 王利娟 曹廷兵 聂海 杨华 《解放军医学杂志》 CAS CSCD 北大核心 2006年第8期794-797,共4页
目的探讨ERK1/2和p38MAPK在脂肪组织生长中的作用。方法Wistar雄性大鼠20只,随机均分为代谢综合征组和正常对照组,观察代谢综合征大鼠的代谢指标及体重、内脏脂肪含量、脂肪细胞大小和组织病理学改变,Westernblot法检测ERK1/2及p38MAPK... 目的探讨ERK1/2和p38MAPK在脂肪组织生长中的作用。方法Wistar雄性大鼠20只,随机均分为代谢综合征组和正常对照组,观察代谢综合征大鼠的代谢指标及体重、内脏脂肪含量、脂肪细胞大小和组织病理学改变,Westernblot法检测ERK1/2及p38MAPK蛋白在脂肪组织中的表达情况。结果代谢综合征大鼠血压、血浆游离脂肪酸、甘油三酯、空腹血糖及胰岛素均显著高于对照组(P<0·01),其体重、腹围、内脏脂肪含量、脂肪系数和各部位脂肪细胞大小亦比对照组显著增高(P<0·01),肝细胞存在脂肪变性。在棕色脂肪组织中ERK1/2蛋白的表达与对照组比较无显著性差异,皮下脂肪和内脏脂肪中ERK1/2蛋白的表达均显著高于对照组(P<0·01),而p38MAPK蛋白的表达在所有脂肪组织中均显著高于对照组(P<0·01)。结论代谢综合征大鼠肥胖特征为腹型肥胖,脂肪组织生长以肥大性增生为主,并存在脂质异位沉着(脂肪肝)。代谢综合征大鼠脂肪组织增生可能与ERK1/2和p38MAPK信号通路激活有关。 展开更多
关键词 代谢综合征 肥胖症 有丝分裂素激活蛋白激酶类 P38 map激酶
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Connectivity map技术在中药研究中的应用 被引量:7
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作者 张晓芳 康永波 +1 位作者 苏君鸿 孔祥阳 《浙江大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2016年第5期543-550,共8页
Connectivity map(CMap)是基于RNA芯片研究药物作用机制和药物重定位的技术与数据资源,主要的优势在于能够高效地对全基因组上的基因表达做整体分析。相对于低通量的药理学、毒理学方法以及色谱技术,该技术能以全面、高通量、准确等优... Connectivity map(CMap)是基于RNA芯片研究药物作用机制和药物重定位的技术与数据资源,主要的优势在于能够高效地对全基因组上的基因表达做整体分析。相对于低通量的药理学、毒理学方法以及色谱技术,该技术能以全面、高通量、准确等优点广泛应用于药物研究。尤其对由多种化合物共同作用发挥疗效的中药,CMap技术可以突破"药物-靶标"测定技术的单一性困难,对中药的作用机制做综合性分析,从分子水平上阐明中药的药物作用机制,有利于开发自主知识产权的中药,也为中药获取国际认可奠定理论基础。本文系统介绍CMap技术在中药研究方面的应用潜力,同时对该技术研究中药的作用机制、药物重定位及鉴别有效成分进行评价,还对推动中药走向国际市场做了具体分析与展望。 展开更多
关键词 CONNECTIVITY map技术 中药 作用机制 药物重定位 有效成分
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益气活血法对脑缺血再灌注大鼠MAP-2及NOGO-A蛋白表达的影响 被引量:5
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作者 郜峦 王键 +3 位作者 胡建鹏 何玲 菅威 唐巍 《辽宁中医药大学学报》 CAS 2013年第5期23-25,共3页
目的:探讨益气活血法对缺血再灌注大鼠的神经保护作用。方法:动物随机分为假手术组、模型组、益气组、活血组、益气活血组,以线栓法阻塞大鼠左侧大脑中动脉复制局灶性脑缺血再灌注模型,缺血2 h再灌注3、7、14 d,用免疫组织化学染色SABC... 目的:探讨益气活血法对缺血再灌注大鼠的神经保护作用。方法:动物随机分为假手术组、模型组、益气组、活血组、益气活血组,以线栓法阻塞大鼠左侧大脑中动脉复制局灶性脑缺血再灌注模型,缺血2 h再灌注3、7、14 d,用免疫组织化学染色SABC法分别检测MAP-2及NOGO-A蛋白的表达。结果:脑缺血再灌注损伤后,模型组MAP-2表达下降,与假手术相比有显著性差异(P<0.05),而益气法、活血法和益气活血法三组治疗组均能显著促进MAP-2的表达(P<0.01)。脑缺血再灌注损伤7 d时,模型组NOGO-A表达与假手术相比有显著性差异(P<0.05)。而益气法、活血法和益气活血法三组治疗组NOGO-A的表达与模型组无显著性差异(P>0.05)。结论:益气活血法能显著促进MAP-2表达,有利于脑缺血后突触重塑和神经细胞轴突的再生,促进脑卒中后神经功能恢复。 展开更多
关键词 益气活血法 神经再生 map-2 NOGO-A
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MAPK在葡萄糖及AngⅡ致血管平滑肌细胞增殖反应中的作用 被引量:3
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作者 张严高 秦永文 +3 位作者 吴弘 王文清 文军慧 李闻捷 《第四军医大学学报》 北大核心 2006年第22期2026-2028,共3页
目的:研究丝裂原活化蛋白激酶(MAPK)信号途径在葡萄糖(GS)和/或血管紧张素Ⅱ(AngⅡ)介导大鼠动脉平滑肌细胞促增殖中的作用及其可能的调控机制.方法:实验以平滑肌细胞蛋白合成速率和蛋白含量为平滑肌细胞增殖的指标,以平滑肌细胞3H-亮... 目的:研究丝裂原活化蛋白激酶(MAPK)信号途径在葡萄糖(GS)和/或血管紧张素Ⅱ(AngⅡ)介导大鼠动脉平滑肌细胞促增殖中的作用及其可能的调控机制.方法:实验以平滑肌细胞蛋白合成速率和蛋白含量为平滑肌细胞增殖的指标,以平滑肌细胞3H-亮氨酸掺入率表示细胞蛋白质合成速率,通过3H-胸苷(3H-TdR)反映平滑肌细胞DNA的代谢及细胞增殖情况;并通过给予PD098059及高浓度葡萄糖(HG)预处理,观察它们对细胞蛋白合成、DNA代谢及细胞增殖的影响.结果:①AngⅡ(10-7mol/L)处理平滑肌细胞,3H-胸苷掺入率明显增高;HG(25×10-3mol/L)处理平滑肌细胞,3H-胸苷掺入率明显增高;AngⅡ+HG处理平滑肌细胞3H-胸苷掺入率显著增高.②AngⅡ增加3H-胸苷掺入的作用可明显被PD098059所抑制;AngⅡ+HG增高3H-胸苷掺入的作用也可被PD098059所抑制.③AngⅡ(10-7mol/L)处理平滑肌细胞,3H-亮氨酸掺入率明显增高;HG(25×10-3mol/L)处理平滑肌细胞,3H-亮氨酸掺入率增加;AngⅡ+HG处理平滑肌细胞3H-亮氨酸掺入率显著增加.④AngⅡ增加3H-亮氨酸掺入的作用可部分被PD098059所抑制;AngⅡ+HG增加3H-亮氨酸掺入的作用可显著被PD098059所抑制.结论:应用MAPK的特异性抑制剂PD098059抑制血管平滑肌细胞的增殖,证明MAPK激活在GS和/或AngⅡ导致平滑肌细胞增殖反应中有重要作用. 展开更多
关键词 丝裂原活化蛋白激酶(mapK) 细胞外信号调节map激酶类(ERKs) 血管紧张素Ⅱ(Ang Ⅱ) 高糖 平滑 血管/细胞学 增殖反应
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布洛芬对淀粉样β蛋白片段1~40引起的大鼠海马p38 MAP激酶信号传导通路及半胱氨酸天冬氨酸蛋白酶级联的影响(英文) 被引量:3
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作者 范莹 金英 +3 位作者 闫恩志 杨菁 宗志红 齐志敏 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2007年第2期81-89,共9页
目的研究布洛芬对淀粉样β蛋白片段1-40 (Aβ1-40)所致大鼠海马损伤的保护作用及作用机制。方法大鼠ig给予布洛芬15 mg.kg-1,连续应用3周,脑室内一次性注射Aβ1-40(5μL,1 mmol.L-1),注射后6h快速取海马CA1区,Western免疫印迹法观察磷... 目的研究布洛芬对淀粉样β蛋白片段1-40 (Aβ1-40)所致大鼠海马损伤的保护作用及作用机制。方法大鼠ig给予布洛芬15 mg.kg-1,连续应用3周,脑室内一次性注射Aβ1-40(5μL,1 mmol.L-1),注射后6h快速取海马CA1区,Western免疫印迹法观察磷酸化丝裂原激活的蛋白激酶的激酶3/6(MKK3/MKK6)、磷酸化丝裂原激活的蛋白激酶p38(p38 MAP激酶)、磷酸化MAPK活化的蛋白激酶2(MAPKAPK2)、磷酸化热休克蛋白p27(Hsp27)、半胱氨酸天冬氨酸蛋白酶(caspase)9, 3,7以及ADP-核糖聚合酶(PARP)的蛋白表达水平的改变。结果脑室内注射Aβ1-40可引起海马CA1区磷酸化的MKK3/MKK6和磷酸化p38 MAP激酶表达明显增加,但可使磷酸化MAPKAPK2和磷酸化的Hsp27表达降低,这些改变伴随有caspase级联的激活。此外,也发现Aβ1-40可使海马CA1区完整的PARP蛋白表达明显减少,而劈切PARP(分子量为89 ku)表达明显增加。布洛芬可明显对抗Aβ1-40引起的磷酸化的MKK3/MKK6和磷酸化p38 MAP激酶表达增加,上调磷酸化MAPKAPK2和磷酸化的Hsp27表达水平,同时抑制Aβ1-40引起的caspase级联激活和PARP的劈切。结论布洛芬通过抑制Aβ1-40引起的磷酸化的MKK3/MKK6和磷酸化p38 MAP激酶表达,明显增加以及上调磷酸化的Hsp27表达水平,对抗Aβ1-40引起的海马的神经细胞损伤。 展开更多
关键词 布洛芬 淀粉样β蛋白 map激酶信号系统 有丝分裂素激活蛋白激酶类 热休克蛋白类 半胱氨酸天冬氨酸蛋白酶
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