The paper mainly talks about the background of S. T. Coleridge and the images of The Rime of the AncientMariner. As for the images, the wind, the albatross and the snake will be thoroughly discussed. There are manydif...The paper mainly talks about the background of S. T. Coleridge and the images of The Rime of the AncientMariner. As for the images, the wind, the albatross and the snake will be thoroughly discussed. There are manydifferent images which also have different meanings and connotations in the context. What＇s more, the poemexpresses the theme of the metabolic relationships between men and nature： harmony, conflict or compromise.展开更多
The narrator of Melvihe’s tale says of Bartleby that he is“one of those beingsof whom nothing is ascertainable.”In other words,Bartleby is a mysterious figureto the narrator.What the narrator has do,is make sense o...The narrator of Melvihe’s tale says of Bartleby that he is“one of those beingsof whom nothing is ascertainable.”In other words,Bartleby is a mysterious figureto the narrator.What the narrator has do,is make sense of Bartleby.展开更多
Generally, Coleridge’s poems are mainly divided into two groups:one is to use common Language to depict commom things and common people; the other is to use unique imagination to describe supernatural things. "T...Generally, Coleridge’s poems are mainly divided into two groups:one is to use common Language to depict commom things and common people; the other is to use unique imagination to describe supernatural things. "The Rime of the Ancient Mariner"belongs to the second group. Some critics blame Coleridge for his lack of morals in this poem, but in fact,this poem contains lots of morals, as Coleridge himself said.Coleridge succeeds in exploring the theme of sin and punishment by his rich imagination and by the depicting of supernatural things. In "The Rime of the Ancient Mariner", Coleridge intentionally built a symbolic world of the supernatural wind, the bird, the sun, the moon ,the ship and the sea. All these symbols serve to develop the theme of Fall and Redemption, and play so important a part in the poem that we even can take it as a symbolic poem. In the following pages, I am going to devote to the functions of the supernatural wind in particular. Coleridge bestows the wind with a great deal of展开更多
One of the most typical Poems in the nineteenth century is The Rime of the Ancient Mariner, written by Samue 1 Taylor Coleridge。This Poem is unique in the history of ballad writing. The eerie incidents of the story t...One of the most typical Poems in the nineteenth century is The Rime of the Ancient Mariner, written by Samue 1 Taylor Coleridge。This Poem is unique in the history of ballad writing. The eerie incidents of the story the spell of th e supernatural, and th e sustained a rchaic style,all con tribu te to make it an unforgettable Poem,PoPular alive with young and old.This article is going to deal with th ree questions:(1)the them of the Poem ( 2 ) the function of the color images and their symbolic representation ?(3) the conceP t of Life一in—Death ?展开更多
Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as T...Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide, and this transposon has been used for mutagenesis of a wide variety of bacteria. However, plasmids for mariner delivery do not necessarily work well in all bacteria. In particular, there are limited tools for functional genomic analysis of Pasteurellaceae species of major veterinary importance, such as swine and cattle pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, respectively. Here, we developed plasmids, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), that allow delivery of mariner into both these pathogens, but which should also be applicable to a wider range of bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and P. multocida that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS. A preliminary screen of 5000 mutants each identified 8 and 14 genes, respectively, that are required for growth under anaerobic conditions. Future high-throughput screening of the generated libraries will facilitate identification of mutants required for growth under different conditions, including in vivo, highlighting key virulence factors and pathways that can be exploited for development of novel therapeutics and vaccines.展开更多
Qingdao Ocean Shipping Mariners College is a College subordinate to China Ocean Shipping (Group) Company under the Ministry of Communications. In our college there are the Department of Marine Navigation,Department of...Qingdao Ocean Shipping Mariners College is a College subordinate to China Ocean Shipping (Group) Company under the Ministry of Communications. In our college there are the Department of Marine Navigation,Department of Marine Engineering, Department of Shipping Management and Department of Foreign Languages, offering speciahies of Marine Navigation, Marine Engine Control, Marine Electrical Equipment Control, Marine Radio Communication, International Shipping Management and International Finance and Accounting. We have 3 types of education: the education of formal college schooling, the training for implementation of the 95 revised STCW Convention and the continuation education. Meanwhile we undertake different training of COSCO’s contracted seafarers overseas and other managerial per-展开更多
When investigating the vortex-induced vibration(VIV)of marine risers,extrapolating the dynamic response on the entire length based on limited sensor measurements is a crucial step in both laboratory experiments and fa...When investigating the vortex-induced vibration(VIV)of marine risers,extrapolating the dynamic response on the entire length based on limited sensor measurements is a crucial step in both laboratory experiments and fatigue monitoring of real risers.The problem is conventionally solved using the modal decomposition method,based on the principle that the response can be approximated by a weighted sum of limited vibration modes.However,the method is not valid when the problem is underdetermined,i.e.,the number of unknown mode weights is more than the number of known measurements.This study proposed a sparse modal decomposition method based on the compressed sensing theory and the Compressive Sampling Matching Pursuit(Co Sa MP)algorithm,exploiting the sparsity of VIV in the modal space.In the validation study based on high-order VIV experiment data,the proposed method successfully reconstructed the response using only seven acceleration measurements when the conventional methods failed.A primary advantage of the proposed method is that it offers a completely data-driven approach for the underdetermined VIV reconstruction problem,which is more favorable than existing model-dependent solutions for many practical applications such as riser structural health monitoring.展开更多
An observer-based adaptive backstepping boundary control is proposed for vibration control of flexible offshore riser systems with unknown nonlinear input dead zone and uncertain environmental disturbances.The control...An observer-based adaptive backstepping boundary control is proposed for vibration control of flexible offshore riser systems with unknown nonlinear input dead zone and uncertain environmental disturbances.The control algorithm can update the control law online through real-time data to make the controller adapt to the environment and improve the control precision.Specifically,based on the adaptive backstepping framework,virtual control laws and Lyapunov functions are designed for each subsystem.Three direction interference observers are designed to track the timevarying boundary disturbance.On this basis,the inverse of the dead zone and linear state transformation are used to compensate for the original system and eliminate the adverse effects of the dead zone.In addition,the stability of the closed-loop system is proven by Lyapunov stability theory.All the system states are bounded,and the vibration offset of the riser converges to a small area of the initial position.Finally,four examples of flexible marine risers are simulated in MATLAB to verify the effectiveness of the proposed controller.展开更多
文摘The paper mainly talks about the background of S. T. Coleridge and the images of The Rime of the AncientMariner. As for the images, the wind, the albatross and the snake will be thoroughly discussed. There are manydifferent images which also have different meanings and connotations in the context. What＇s more, the poemexpresses the theme of the metabolic relationships between men and nature： harmony, conflict or compromise.
文摘The narrator of Melvihe’s tale says of Bartleby that he is“one of those beingsof whom nothing is ascertainable.”In other words,Bartleby is a mysterious figureto the narrator.What the narrator has do,is make sense of Bartleby.
文摘Generally, Coleridge’s poems are mainly divided into two groups:one is to use common Language to depict commom things and common people; the other is to use unique imagination to describe supernatural things. "The Rime of the Ancient Mariner"belongs to the second group. Some critics blame Coleridge for his lack of morals in this poem, but in fact,this poem contains lots of morals, as Coleridge himself said.Coleridge succeeds in exploring the theme of sin and punishment by his rich imagination and by the depicting of supernatural things. In "The Rime of the Ancient Mariner", Coleridge intentionally built a symbolic world of the supernatural wind, the bird, the sun, the moon ,the ship and the sea. All these symbols serve to develop the theme of Fall and Redemption, and play so important a part in the poem that we even can take it as a symbolic poem. In the following pages, I am going to devote to the functions of the supernatural wind in particular. Coleridge bestows the wind with a great deal of
文摘One of the most typical Poems in the nineteenth century is The Rime of the Ancient Mariner, written by Samue 1 Taylor Coleridge。This Poem is unique in the history of ballad writing. The eerie incidents of the story the spell of th e supernatural, and th e sustained a rchaic style,all con tribu te to make it an unforgettable Poem,PoPular alive with young and old.This article is going to deal with th ree questions:(1)the them of the Poem ( 2 ) the function of the color images and their symbolic representation ?(3) the conceP t of Life一in—Death ?
基金This work was supported by a Longer and Larger(LoLa)grant from the Biotechnology and Biological Sciences Research Council(BBSRC,grant numbers BB/G020744/1,BB/G019177/1,BB/G019274/1 and BB/G018553/1)the UK Department for Environment,Food and Rural Affairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.Funding for LZ was provided by the BBSRC(grant number BB/C508193/1)The funders had no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.
文摘Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide, and this transposon has been used for mutagenesis of a wide variety of bacteria. However, plasmids for mariner delivery do not necessarily work well in all bacteria. In particular, there are limited tools for functional genomic analysis of Pasteurellaceae species of major veterinary importance, such as swine and cattle pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, respectively. Here, we developed plasmids, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), that allow delivery of mariner into both these pathogens, but which should also be applicable to a wider range of bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and P. multocida that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS. A preliminary screen of 5000 mutants each identified 8 and 14 genes, respectively, that are required for growth under anaerobic conditions. Future high-throughput screening of the generated libraries will facilitate identification of mutants required for growth under different conditions, including in vivo, highlighting key virulence factors and pathways that can be exploited for development of novel therapeutics and vaccines.
文摘Qingdao Ocean Shipping Mariners College is a College subordinate to China Ocean Shipping (Group) Company under the Ministry of Communications. In our college there are the Department of Marine Navigation,Department of Marine Engineering, Department of Shipping Management and Department of Foreign Languages, offering speciahies of Marine Navigation, Marine Engine Control, Marine Electrical Equipment Control, Marine Radio Communication, International Shipping Management and International Finance and Accounting. We have 3 types of education: the education of formal college schooling, the training for implementation of the 95 revised STCW Convention and the continuation education. Meanwhile we undertake different training of COSCO’s contracted seafarers overseas and other managerial per-
基金financially supported by the National Natural Science Foundation of China(Grant Nos.51109158,U2106223)the Science and Technology Development Plan Program of Tianjin Municipal Transportation Commission(Grant No.2022-48)。
文摘When investigating the vortex-induced vibration(VIV)of marine risers,extrapolating the dynamic response on the entire length based on limited sensor measurements is a crucial step in both laboratory experiments and fatigue monitoring of real risers.The problem is conventionally solved using the modal decomposition method,based on the principle that the response can be approximated by a weighted sum of limited vibration modes.However,the method is not valid when the problem is underdetermined,i.e.,the number of unknown mode weights is more than the number of known measurements.This study proposed a sparse modal decomposition method based on the compressed sensing theory and the Compressive Sampling Matching Pursuit(Co Sa MP)algorithm,exploiting the sparsity of VIV in the modal space.In the validation study based on high-order VIV experiment data,the proposed method successfully reconstructed the response using only seven acceleration measurements when the conventional methods failed.A primary advantage of the proposed method is that it offers a completely data-driven approach for the underdetermined VIV reconstruction problem,which is more favorable than existing model-dependent solutions for many practical applications such as riser structural health monitoring.
基金financially supported by the Sichuan Science and Technology Program(Grant No.2023NSFSC1980)。
文摘An observer-based adaptive backstepping boundary control is proposed for vibration control of flexible offshore riser systems with unknown nonlinear input dead zone and uncertain environmental disturbances.The control algorithm can update the control law online through real-time data to make the controller adapt to the environment and improve the control precision.Specifically,based on the adaptive backstepping framework,virtual control laws and Lyapunov functions are designed for each subsystem.Three direction interference observers are designed to track the timevarying boundary disturbance.On this basis,the inverse of the dead zone and linear state transformation are used to compensate for the original system and eliminate the adverse effects of the dead zone.In addition,the stability of the closed-loop system is proven by Lyapunov stability theory.All the system states are bounded,and the vibration offset of the riser converges to a small area of the initial position.Finally,four examples of flexible marine risers are simulated in MATLAB to verify the effectiveness of the proposed controller.
