Various behaviors of cancer cells are strongly influenced by their interaction with extracellular matrices(ECM).We investigate how this interaction may be influenced if the cancer cells’ability of secreting matrix me...Various behaviors of cancer cells are strongly influenced by their interaction with extracellular matrices(ECM).We investigate how this interaction may be influenced if the cancer cells’ability of secreting matrix metalloproteinases(MMPs)to degrade ECM is inhibited by adding the MMP inhibitor.We useMDA-MB-231-GFP cells as model cells and use matrigel to mimic ECM.It is found that the added MMP inhibitor significantly reduces the migration speed of cancer cells covered by matrigel but has little influence on the migration persistence and shape factor of the cells and that with the MMP inhibitor added the presence of matrigel on the top has no influence on the migration speed of the cells but increases the cells’shape factor and migration persistence.展开更多
The interaction between extracellular matrices and cancer cells plays an important role in regulating cancer cell behaviors. In this article, we use matrigel to mimic extracellular matrices and investigate experimenta...The interaction between extracellular matrices and cancer cells plays an important role in regulating cancer cell behaviors. In this article, we use matrigel to mimic extracellular matrices and investigate experimentally how matrigel influences the shape and dynamics of breast cancer cells(MDA-MB-231-GFP cells). We find that matrigel facilitates cancer cells' migration and shape deformation. The influences of the matrigel concentration are also reported.展开更多
The human endometrial adenocarcinoma cell liueIskikawa cells suspended either in Matrigel or MEM buffer medium were injected subcutaneously to nude mice (N=10 mice/group);and the fresh human cervical cancer tissues ob...The human endometrial adenocarcinoma cell liueIskikawa cells suspended either in Matrigel or MEM buffer medium were injected subcutaneously to nude mice (N=10 mice/group);and the fresh human cervical cancer tissues obtained directly from the surgery were coated by Matrigel and transplanted into nude mouse. The purpose of these studies were to see if Matrigel could enhance the human uteriue cancers' proliferation and differentiation in vivo.The results showed that when cancer cells or tissues implanted into nude mice in Matrigel, the xenografts could turn out earlier, proliferate faster and kept the original differentiation better.It is suggested that Matrigel is an ideal agent in establishing animal models of human uterine cancer.展开更多
文摘目的以肿瘤坏死因子-α(TNF-α)诱导的人脐静脉内皮细胞(HUVEC)为研究对象,探讨罗仙子(Musca Domestica)抗炎活性部位在体外对HUVEC增殖及Matrigel小管形成的影响。方法将构建的HUVEC细胞分为正常对照组、模型组及罗仙子活性部位处理组,制备罗仙子抗炎活性部位,采用流式细胞术结合ModFit LT软件分析,明确罗仙子抗炎活性部位对HUVEC增殖的影响;采用Matrigel小管形成实验结合Image Pro Plus软件分析,明确罗仙子抗炎活性部位对HUVEC血管形成的影响。结果相对模型组,罗仙子抗炎活性部位处理组HUVEC增殖得到显著的抑制,增殖系数降低明显(P<0.01),同时,该处理组Matrigel小管形成的数量、面积和长度显著减少(P<0.01)。结论罗仙子抗炎活性部位对TNF-α诱导HUVEC增殖及Matrigel小管形成具有抑制作用。
基金Supported by The Shanghai Municipal Natural Science Foundation,No.11ZR1405500the Shanghai Municipal Science and Technology Commission grant,No.13140902401
文摘AIM: To establish an orthotopic mouse model of pancreatic cancer that mimics the pathological features of exocrine pancreatic adenocarcinoma.
基金National Natural Science Foundation of China(Grant No.11774394)the Key Research Program of Frontier Sciences of Chinese Academy of Sciences(Grant No.QYZDB-SSW-SYS003)the K.C.Wong Education Foundation.
文摘Various behaviors of cancer cells are strongly influenced by their interaction with extracellular matrices(ECM).We investigate how this interaction may be influenced if the cancer cells’ability of secreting matrix metalloproteinases(MMPs)to degrade ECM is inhibited by adding the MMP inhibitor.We useMDA-MB-231-GFP cells as model cells and use matrigel to mimic ECM.It is found that the added MMP inhibitor significantly reduces the migration speed of cancer cells covered by matrigel but has little influence on the migration persistence and shape factor of the cells and that with the MMP inhibitor added the presence of matrigel on the top has no influence on the migration speed of the cells but increases the cells’shape factor and migration persistence.
基金support of the CAS Key Lab of Soft Matter Physics
文摘The interaction between extracellular matrices and cancer cells plays an important role in regulating cancer cell behaviors. In this article, we use matrigel to mimic extracellular matrices and investigate experimentally how matrigel influences the shape and dynamics of breast cancer cells(MDA-MB-231-GFP cells). We find that matrigel facilitates cancer cells' migration and shape deformation. The influences of the matrigel concentration are also reported.
文摘The human endometrial adenocarcinoma cell liueIskikawa cells suspended either in Matrigel or MEM buffer medium were injected subcutaneously to nude mice (N=10 mice/group);and the fresh human cervical cancer tissues obtained directly from the surgery were coated by Matrigel and transplanted into nude mouse. The purpose of these studies were to see if Matrigel could enhance the human uteriue cancers' proliferation and differentiation in vivo.The results showed that when cancer cells or tissues implanted into nude mice in Matrigel, the xenografts could turn out earlier, proliferate faster and kept the original differentiation better.It is suggested that Matrigel is an ideal agent in establishing animal models of human uterine cancer.
