目的观察吡格列酮对高脂血症大鼠主动脉基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)表达的影响。方法 26只雄性SD大鼠随机分成普通饲料组(n=9)和高脂饲料组(n=17),分笼饲养,12周后检测空腹血脂水平,以确定高脂饲料组高脂血症大...目的观察吡格列酮对高脂血症大鼠主动脉基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)表达的影响。方法 26只雄性SD大鼠随机分成普通饲料组(n=9)和高脂饲料组(n=17),分笼饲养,12周后检测空腹血脂水平,以确定高脂饲料组高脂血症大鼠造模是否成功;再将成模大鼠随机分成模型组(n=8)和吡格列酮组(n=9)。第13周起予吡格列酮组大鼠予吡格列酮混悬液连续灌胃4周,余2组用相应量的蒸馏水灌胃4周,平行检测2组大鼠血脂水平,免疫组化检测主动脉MMP-2、MMP-9表达,并用Image-Pro Plus 6.0图像分析系统分析染色结果。结果高脂饲料喂养12周大鼠总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白胆固醇(LDL-C)水平较对照组明显升高(P<0.05),表明造模成功。吡格列酮组主动脉MMP-2、MMP-9表达水平较之模型组明显降低(P<0.05)。结论吡格列酮能通过抑制主动脉MMP-2、MMP-9表达,有利于斑块的稳定性增加。展开更多
Objective： To investigate the effects of Biejia Ruangan Tablet （复方鳖甲软肝片, BRT）- containing serum on the expression of matrix metalloproteinase （MMP-9） and tissue inhibitor of metalloproteinase （TIMP-1） in...Objective： To investigate the effects of Biejia Ruangan Tablet （复方鳖甲软肝片, BRT）- containing serum on the expression of matrix metalloproteinase （MMP-9） and tissue inhibitor of metalloproteinase （TIMP-1） in cultured renal interstitial fibroblasts. Methods： Different BRT-containing sera were prepared by gastric gavages to rats with the high-dose （7 g/kg）, mid-dose （3.5 g/kg）, and low-dose （1.75 g/kg） BRT respectively. The expression of extracellular matrix in NRK-49F cells was induced by treatment with human transforming growth factor-β1 （recombined human TGF-β 1）, and BRT-containing serum. Western blotting and Northern blotting were used to measure type I and III procollagen, MMP-9, and TIMP-1. Results： The high dose BRT-containing serum could decrease the type Ⅰ and Ⅲ procollagen gene expression which boosted by TGF- 13 1, at the same time cut down TIMP-1 protein and gene expression which increased by TGF- β1 （P〈0.05）. Treatment of cells with recombined human TGF-β 1 had no significant effect on MMP-9 expression and BRT- containing serum also had no effect on MMP-9 expression. Conclusions： High dose BRT has anti-fibrosis effects in NRK-49F cells, as indicated by its inhibition of type Ⅰ and Ⅲ procollagen and TIMP-1 expression.展开更多
文摘目的观察吡格列酮对高脂血症大鼠主动脉基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)表达的影响。方法 26只雄性SD大鼠随机分成普通饲料组(n=9)和高脂饲料组(n=17),分笼饲养,12周后检测空腹血脂水平,以确定高脂饲料组高脂血症大鼠造模是否成功;再将成模大鼠随机分成模型组(n=8)和吡格列酮组(n=9)。第13周起予吡格列酮组大鼠予吡格列酮混悬液连续灌胃4周,余2组用相应量的蒸馏水灌胃4周,平行检测2组大鼠血脂水平,免疫组化检测主动脉MMP-2、MMP-9表达,并用Image-Pro Plus 6.0图像分析系统分析染色结果。结果高脂饲料喂养12周大鼠总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白胆固醇(LDL-C)水平较对照组明显升高(P<0.05),表明造模成功。吡格列酮组主动脉MMP-2、MMP-9表达水平较之模型组明显降低(P<0.05)。结论吡格列酮能通过抑制主动脉MMP-2、MMP-9表达,有利于斑块的稳定性增加。
基金Supported by the National Science Foundation of China(No.30130220 and No.30873345)National Natural Science Found for Innovative Research Groups Science Foundation of China(No.30121005)
文摘Objective： To investigate the effects of Biejia Ruangan Tablet （复方鳖甲软肝片, BRT）- containing serum on the expression of matrix metalloproteinase （MMP-9） and tissue inhibitor of metalloproteinase （TIMP-1） in cultured renal interstitial fibroblasts. Methods： Different BRT-containing sera were prepared by gastric gavages to rats with the high-dose （7 g/kg）, mid-dose （3.5 g/kg）, and low-dose （1.75 g/kg） BRT respectively. The expression of extracellular matrix in NRK-49F cells was induced by treatment with human transforming growth factor-β1 （recombined human TGF-β 1）, and BRT-containing serum. Western blotting and Northern blotting were used to measure type I and III procollagen, MMP-9, and TIMP-1. Results： The high dose BRT-containing serum could decrease the type Ⅰ and Ⅲ procollagen gene expression which boosted by TGF- 13 1, at the same time cut down TIMP-1 protein and gene expression which increased by TGF- β1 （P〈0.05）. Treatment of cells with recombined human TGF-β 1 had no significant effect on MMP-9 expression and BRT- containing serum also had no effect on MMP-9 expression. Conclusions： High dose BRT has anti-fibrosis effects in NRK-49F cells, as indicated by its inhibition of type Ⅰ and Ⅲ procollagen and TIMP-1 expression.
