To investigate the specific creep behavior of ultra-deep buried salt during oil and gas exploitation,a set of triaxial creep experiments was conducted at elevated temperatures with constant axial pressure and unloadin...To investigate the specific creep behavior of ultra-deep buried salt during oil and gas exploitation,a set of triaxial creep experiments was conducted at elevated temperatures with constant axial pressure and unloading confining pressure conditions.Experimental results show that the salt sample deforms more significantly with the increase of applied temperature and deviatoric loading.The accelerated creep phase is not occurring until the applied temperature reaches 130℃,and higher temperature is beneficial to the occurrence of accelerated creep.To describe the specific creep behavior,a novel three-dimensional(3D)creep constitutive model is developed that incorporates the thermal and mechanical variables into mechanical elements.Subsequently,the standard particle swarm optimization(SPSO)method is adopted to fit the experimental data,and the sensibility of key model parameters is analyzed to further illustrate the model function.As a result,the model can accurately predict the creep behavior of salt under the coupled thermo-mechanical effect in deep-buried condition.Based on the research results,the creep mechanical behavior of wellbore shrinkage is predicted in deep drilling projects crossing salt layer,which has practical implications for deep rock mechanics problems.展开更多
The changes of hydrogen peroxide (H2O2) metabolism and antioxidant enzyme activities in a hybrid poplar (Populus simonii xp. pyramidalis 'Opera 8277') in response to rnechanical damage (MD) and herbivore wound...The changes of hydrogen peroxide (H2O2) metabolism and antioxidant enzyme activities in a hybrid poplar (Populus simonii xp. pyramidalis 'Opera 8277') in response to rnechanical damage (MD) and herbivore wounding (HW) were investigated to determine whether H2O2 could function as the secondary messenger in the signaling of systemic resistance. Results show that H2O2 was generated in wounded leaves through MD and HW treatments and systemically in unwounded leaves around the wounded leaves. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were also enhanced. However, the H2O2 accumulation and antioxidant enzyme activities were inhibited in MD leaves through the pretreatment with DPI (which is a specific inhibitor of NADPH oxidase). The results of this study suggest that H2O2 could be systemically induced by MD and HW treatments, and H2O2 metabolism was closely related to the change in SOD, APX and CAT activities. A high level of antioxidant enzymes could decrease membrane lipid peroxidation levels and effectively induce plant defense responses.展开更多
Laser technology holds significant promise for enhancing rock-breaking efficiency.Experimental investigations were carried out on sandstone subjected to laser radiation,aiming to elucidate its response mechanism to su...Laser technology holds significant promise for enhancing rock-breaking efficiency.Experimental investigations were carried out on sandstone subjected to laser radiation,aiming to elucidate its response mechanism to such radiation.The uniaxial compressive strength of sandstone notably decreases by 22.1%–54.7%following exposure to a 750 W laser for 30 s,indicating a substantial weakening effect.Furthermore,the elastic modulus and Poisson ratio of sandstone exhibit an average decrease of 33.7%and 25.9%,respectively.Simultaneously,laser radiation reduces the brittleness of sandstone,increases the dissipated energy proportion,and shifts the failure mode from tensile to tension-shear composite failure.Following laser radiation,both the number and energy of acoustic emission events in the sandstone register a substantial increase,with a more dispersed distribution of these events.In summary,laser radiation induces notable damage to the mechanical properties of sandstone,leading to a substantial decrease in elastic energy storage capacity.Laser rock breaking technology is expected to be applied in hard rock breaking engineering to significantly reduce the difficulty of rock breaking and improve rock breaking efficiency.展开更多
This study aims to investigate the mechanical response and acoustic emission(AE)characteristic of pre-flawed sandstone under both monotonic and multilevel constant-amplitude cyclic loads.Specifically,we explored how c...This study aims to investigate the mechanical response and acoustic emission(AE)characteristic of pre-flawed sandstone under both monotonic and multilevel constant-amplitude cyclic loads.Specifically,we explored how coplanar flaw angle and load type impact the strength and deformation behavior and microscopic damage mechanism.Results indicated that being fluctuated before rising with increasing fissure angle under monotonic loading,the peak strength of the specimen first increased slowly and then steeply under cyclic loading.The effect of multilevel cyclic loading on the mechanical parameters was more significant.For a single fatigue stage,the specimen underwent greater deformation in early cycles,which subsequently stabilized.Similar variation pattern was also reflected by AE count/energy/b-value.Crack behaviors were dominated by the fissure angle and load type and medium-scale crack accounted for 74.83%–86.44%of total crack.Compared with monotonic loading,crack distribution of specimen under cyclic loading was more complicated.Meanwhile,a simple model was proposed to describe the damage evolution of sandstone under cyclic loading.Finally,SEM images revealed that the microstructures at the fracture were mainly composed of intergranular fracture,and percentage of transgranular fracture jumped under cyclic loading due to the rapid release of elastic energy caused by high loading rate.展开更多
Rock has mechanical characteristics and a fracture damage mechanism that are closely related to its loading history and loading path. The mechanical properties, fracture damage features, acoustic emission(AE) characte...Rock has mechanical characteristics and a fracture damage mechanism that are closely related to its loading history and loading path. The mechanical properties, fracture damage features, acoustic emission(AE) characteristics, and strain energy evolution of the Beishan shallow-layer granite used in triaxial unloading tests were investigated in this study. Three groups of triaxial tests, namely, conventional triaxial compression test(Group Ⅰ), maintaining deviatoric stress synchronously unloading confining pressure test(Group Ⅱ), and loading axial pressure synchronously unloading confining pressure test(Group Ⅲ), were carried out for the cylindrical granite specimens. AE monitoring device was utilized in these tests to determine the degree to which the AE waves and AE events reflected the degree of rock damage. In addition, the crack stress thresholds of the specimens were determined by volumetric strain method and AE parameter method, and strain energy evolution of the rock was explored in different damage stages. The results show that the shallow-layer granite experiences brittle failure during the triaxial loading test and unloading test, and the rock has a greater damage degree during the unloading test. The crack stress thresholds of these samples vary greatly between tests, but the threshold ratios of all samples are similar in the same crack damage stage. The Mogi-Coulomb strength criterion can better describe the unloading failure strength of the rock. The evolution of the AE parameter characteristics and strain energy differs between the specimens used in different stress path tests. The dissipative strain energy is the largest in Group Ⅱ and the smallest in Group Ⅰ.展开更多
Aiming to analyze the damage mechanism of UTAO from the perspective of meso-mechanical mechanism using discrete element method(DEM),we conducted study of diseases problems of UTAO in several provinces in China,and fou...Aiming to analyze the damage mechanism of UTAO from the perspective of meso-mechanical mechanism using discrete element method(DEM),we conducted study of diseases problems of UTAO in several provinces in China,and found that aggregate spalling was one of the main disease types of UTAO.A discrete element model of UTAO pavement structure was constructed to explore the meso-mechanical mechanism of UTAO damage under the influence of layer thickness,gradation,and bonding modulus.The experimental results show that,as the thickness of UTAO decreasing,the maximum value and the mean value of the contact force between all aggregate particles gradually increase,which leads to aggregates more prone to spalling.Compared with OGFC-5 UTAO,AC-5 UTAO presents smaller maximum and average values of all contact forces,and the loading pressure in AC-5 UTAO is fully diffused in the lateral direction.In addition,the increment of pavement modulus strengthens the overall force of aggregate particles inside UTAO,resulting in aggregate particles peeling off more easily.The increase of bonding modulus changes the position where the maximum value of the tangential force appears,whereas has no effect on the normal force.展开更多
Ground-based tests are important for studying hypervelocity impact(HVI)damage to spacecraft pressure vessels in the orbital debris environment.We analyzed the damage to composite overwrapped pressure vessels(COPVs)in ...Ground-based tests are important for studying hypervelocity impact(HVI)damage to spacecraft pressure vessels in the orbital debris environment.We analyzed the damage to composite overwrapped pressure vessels(COPVs)in the HVI tests and classified the damage into non-catastrophic damage and catastrophic damage.We proposed a numerical simulation method to further study non-catastrophic damage and revealed the characteristics and mechanisms of non-catastrophic damage affected by impact conditions and internal pressures.The fragments of the catastrophically damaged COPVs were collected after the tests.The crack distribution and propagation process of the catastrophic ruptures of the COPVs were analyzed.Our findings contribute to understanding the damage characteristics and mechanisms of COPVs by HVIs.展开更多
The incorporation of commercial flame retardants into fiber-reinforced polymer(FRP)composites has been proposed as a potential solution to improve the latter’s poor flame resistance.However,this approach often poses ...The incorporation of commercial flame retardants into fiber-reinforced polymer(FRP)composites has been proposed as a potential solution to improve the latter’s poor flame resistance.However,this approach often poses a challenge,as it can adversely affect the mechanical properties of the FRP.Thus,balancing the need for improved flame resistance with the preservation of mechanical integrity remains a complex issue in FRP research.Addressing this critical concern,this study introduces a novel additive system featuring a combination of one-dimensional(1D)hollow tubular structured halloysite nanotubes(HNTs)and two-dimensional(2D)polygonal flake-shaped nano kaolinite(NKN).By employing a 1D/2D hybrid kaolinite nanoclay system,this research aims to simultaneously improve the flame retardancy and mechanical properties.This innovative approach offers several advantages.During combustion and pyrolysis processes,the 1D/2D hybrid kaolinite nanoclay system proves effective in reducing heat release and volatile leaching.Furthermore,the system facilitates the formation of reinforcing skeletons through a crosslinking mechanism during pyrolysis,resulting in the development of a compact char layer.This char layer acts as a protective barrier,enhancing the material’s resistance to heat and flames.In terms of mechanical properties,the multilayered polygonal flake-shaped 2D NKN plays a crucial role by impeding the formation of cracks that typically arise from vulnerable areas,such as adhesive phase particles.Simultaneously,the 1D HNT bridges these cracks within the matrix,ensuring the structural integrity of the composite material.In an optimal scenario,the homogeneously distributed 1D/2D hybrid kaolinite nanoclays exhibit remarkable results,with a 51.0%improvement in mode II fracture toughness(GIIC),indicating increased resistance to crack propagation.In addition,there is a 34.5%reduction in total heat release,signifying improved flame retardancy.This study represents a significant step forward in the field of composite materials.The innovative use of hybrid low-dimensional nanomaterials offers a promising avenue for the development of multifunctional composites.By carefully designing and incorporating these nanoclays,researchers can potentially create a new generation of FRP composites that excel in both flame resistance and mechanical strength.展开更多
Underground rock dynamic disasters are becoming more severe due to the increasing depth of human operations underground.Underground temperature and pressure conditions contribute significantly to these disasters.There...Underground rock dynamic disasters are becoming more severe due to the increasing depth of human operations underground.Underground temperature and pressure conditions contribute significantly to these disasters.Therefore,it is important to understand the coupled thermo-mechanical(TM)behaviour of rocks for the long-term safety and maintenance of underground tunnelling and mining.Moreover,investigation of the damage,strength and failure characteristics of rocks under triaxial stress conditions is important to avoid underground rock disasters.In this study,based on Weibull distribution and Lemaitre's strain equivalent principle,a statistical coupled TM constitutive model for sandstone was established under high temperature and pressure conditions.The triaxial test results of sandstone under different temperature and pressure conditions were used to validate the model.The proposed model was in good agreement with the experimental results up to 600℃.The total TM damage was decreased with increasing temperature,while it was increased with increasing confining pressure.The model's parameters can be calculated using conventional laboratory test results.展开更多
To study the mechanical and damage evolution properties of sandstone under triaxial compression, we analyzed the stress strain curve characteristics, deformation and strength properties, and failure process and charac...To study the mechanical and damage evolution properties of sandstone under triaxial compression, we analyzed the stress strain curve characteristics, deformation and strength properties, and failure process and characteristics of sandstone samples under different stress states. The experimental results reveal that peak strength, residual strength, elasticity modulus and deformation modulus increase linearly with confining pressure, and failure models transform from fragile failure under low confining pressure to ductility failure under high confining pressure. Macroscopic failure forms of samples under uniaxial compression were split failure parallel to the axis of samples, while macroscopic failure forms under uniaxial compression were shear failure, the shear failure angle of which decreased linearly with confin- ing pressure. There were significant volume dilatation properties in the loading process of sandstone under different confining pressures, and we analyzed the damage evolution properties of samples based on acoustic emission damage and volumetric dilatation damage, and established damage constitutive model, realizing the real-time Quantitative evaluation of samnles damage state in loading process.展开更多
To study the damage mechanisms of anhydrite rock under freeze-thaw cycles, the physicalmechanical properties and the microcracking activities of anhydrite rock were investigated through mass variation, nuclear magneti...To study the damage mechanisms of anhydrite rock under freeze-thaw cycles, the physicalmechanical properties and the microcracking activities of anhydrite rock were investigated through mass variation, nuclear magnetic resonance, scanning electron microscope tests, and uniaxial compression combined with acoustic emission(AE) tests. Results show that with the increase of freeze-thaw processes,the mass, uniaxial compression strength, and elastic modulus of the anhydrite specimens decrease while the porosity and plasticity characteristics increase.For example, after 120 cycles, the uniaxial compression strength and elastic modulus decrease by 46.54% and 60.16%, and the porosity increase by 75%. Combined with the evolution trend of stressstrain curves and the detected events, three stages were labeled to investigate the AE characteristics in freeze-thaw weathered anhydrite rock. It is found that with the increase of freeze-thaw cycles, the proportions of AE counts in stage Ⅰ and stage Ⅱ show a decaying exponential trend. Contrarily, the proportion of AE counts in stage Ⅲ displays an exponential ascending trend. Meanwhile, as the freeze-thaw cycles increase, the low-frequency AE signals increase while the intermediate-frequency AE signals decrease. After 120 cycles, the proportion of low-frequency AE signals increases by 168.95%, and the proportion of intermediate-frequency AE signals reduces by 81.14%. It is concluded that the microtensile cracking events occupy a dominant position during the loading process. With the increase of freeze-thaw cycles, the b value of samples decreases.After 120 cycles, b value decreases by 27.2%, which means that the proportion of cracking events in rocks with small amplitude decreases. Finally, it is proposed that the freeze-thaw damage mechanism of anhydrite is also characterized by the water chemical softening effect.展开更多
To study the damage mechanism of multi‐anchor piles in tunnel crossing landslide area under earthquake,the damping performance of multi‐anchor piles was discussed.The energy dissipation springs were used as the opti...To study the damage mechanism of multi‐anchor piles in tunnel crossing landslide area under earthquake,the damping performance of multi‐anchor piles was discussed.The energy dissipation springs were used as the optimization device of the anchor head to carry out the shaking table comparison test on the reinforced slope.The Hilbert spectrum and Hilbert marginal spectrum were proposed to analyze the seismic damage mechanism of the multi‐anchor piles,and the peak Fourier spectrum amplitude(PFSA)was used to verify the effectiveness of the method.The results show that the seismic energy is concentrated in the high‐frequency component(30-40Hz)of the Hilbert spectrum and the low‐frequency component(12-30 Hz)of the marginal spectrum.This indicates that they can be combined with the distribution law of the PFSA to identify the overall and local dynamic responses of the multi‐anchored piles,respectively.The stretchable deformation of the energy‐dissipation springs improves the coordination of the multi‐anchor piles,resulting in better pile integrity.The damage mechanism of the multi‐anchor piles is elucidated based on the energy method:local damage at the top and middle areas of the multi‐anchor piles is mainly caused by the low‐frequency component(12-30 Hz)of the marginal spectrum under the action of 0.15g and 0.20g seismic intensities.As the seismic intensity increases to 0.30g,the dynamic response of the slope is further amplified by the high‐frequency component(30-40 Hz)of the Hilbert energy spectrum,which leads to the overall damage of the multi‐anchor piles.展开更多
By using the technique of the split Hopkinson pressure bar( SHPB),impact tests at different stress wavelengths( 0. 8-2. 0 m) and strain rates( 20-120 s^(-1)) were conducted to study the dynamic mechanical prop...By using the technique of the split Hopkinson pressure bar( SHPB),impact tests at different stress wavelengths( 0. 8-2. 0 m) and strain rates( 20-120 s^(-1)) were conducted to study the dynamic mechanical properties and damage accumulation evolution lawof granite. Test results showthat the dynamic compressive strength and strain rate of granite have a significantly exponential correlation;the relationship between peak strain and strain rate is approximately linear,and the increase of wavelengths generally makes the level of peak strain uplift. The multiple-impacts test at a lowstrain rate indicates that at the same wavelength,the cumulative damage of granite shows an exponential increasing form with the increase of strain rate; when keeping the increase of strain rate constant and increasing the stress wavelength,the damage accumulation effect of granite is intensified and still shows an exponential increasing form; under the effect of multiple impacts,the damage development trend of granite is similar overall,but the increase rate is accelerating. Therefore the damage evolution model was established on the basis of the exponential function while the physical meaning of parameters in the model was determined. The model can reflect the effect of the wave parameters and multiple impacts. The validity of the model and the physical meaning of the parameters were verified by the test,which further offer a reference for correlational research and engineering application for the granite.展开更多
The process of thermal stress damage during 1080 nm laser ablation of single-crystal germanium was recorded in real time using a high-speed charge-coupled device.A three-dimensional finite element numerical model base...The process of thermal stress damage during 1080 nm laser ablation of single-crystal germanium was recorded in real time using a high-speed charge-coupled device.A three-dimensional finite element numerical model based on Fourier's heat conduction equation,Hooke's law and the Alexander–Hasson equation was developed to analyze the thermal stress damage mechanism involved.The damage morphology of the ablated samples was observed using an optical microscope.The results show that the cooling process has an important influence on fracture in the laser-irradiated region of single-crystal germanium.Fracture is the result of a combination of thermal stress and reduction in local yield strength.展开更多
Full-length grouted bolts play a crucial role in geotechnical engineering thanks to their excellent stability.However,few studies have been concerned with the degrading performance of grouted rock bolts caused by exte...Full-length grouted bolts play a crucial role in geotechnical engineering thanks to their excellent stability.However,few studies have been concerned with the degrading performance of grouted rock bolts caused by extensive and continuous heat conduction from surrounding rocks in high-geothermal tunnels buried more than 100 m(temperature from 28C to 100C).To investigate the damage mechanism,we examined the time-varying behaviors of grouted rock bolts in both constant and variable temperature curing environments and their damage due to the coupling effects of high temperature and humidity through mechanical and micro-feature tests,including uniaxial compression test,pull-out test,computed tomography(CT)scans,X-ray diffraction(XRD)test,thermogravimetric analysis(TGA),etc.,and further analyzed the relationship between grout properties and anchorage capability.In order to facilitate a rapid assessment and control of the anchorage performance of anchors in different conditions,results of the interface bond degradation tests were correlated to environment parameters based on the damage model of interfacial bond stress proposed.Accordingly,a thermal hazard classification criterion for anchorage design in high-geothermal tunnels was suggested.Based on the reported results,although high temperature accelerated the early-stage hydration reaction of grouting materials,it affected the distribution and quantity of hydration products by inhibiting hydration degree,thus causing mechanical damage to the anchorage system.There was a significant positive correlation between the strength of the grouting material and the anchoring force.Influenced by the changes in grout properties,three failure patterns of rock bolts typically existed.Applying a hot-wet curing regime results in less reduction in anchorage force compared to the hot-dry curing conditions.The findings of this study would contribute to the design and investigations of grouted rock bolts in high-geothermal tunnels.展开更多
The stability of coal walls(pillars)can be seriously undermined by diverse in-situ dynamic disturbances.Based on a 3D par-ticle model,this work strives to numerically replicate the major mechanical responses and acous...The stability of coal walls(pillars)can be seriously undermined by diverse in-situ dynamic disturbances.Based on a 3D par-ticle model,this work strives to numerically replicate the major mechanical responses and acoustic emission(AE)behaviors of coal samples under multi-stage compressive cyclic loading with different loading and unloading rates,which is termed differential cyclic loading(DCL).A Weibull-distribution-based model with heterogeneous bond strengths is constructed by both considering the stress-strain relations and AE parameters.Six previously loaded samples were respectively grouped to indicate two DCL regimes,the damage mechanisms for the two groups are explicitly characterized via the time-stress-dependent variation of bond size multiplier,and it is found the two regimes correlate with distinct damage patterns,which involves the competition between stiffness hardening and softening.The numerical b-value is calculated based on the mag-nitudes of AE energy,the results show that both stress level and bond radius multiplier can impact the numerical b-value.The proposed numerical model succeeds in replicating the stress-strain relations of lab data as well as the elastic-after effect in DCL tests.The effect of damping on energy dissipation and phase shift in numerical model is summarized.展开更多
This work proposes a numerical investigation on the effects of damage on the structural response of Reinforced Concrete(RC)bridge structures commonly adopted in highway and railway networks.An effective three-dimensio...This work proposes a numerical investigation on the effects of damage on the structural response of Reinforced Concrete(RC)bridge structures commonly adopted in highway and railway networks.An effective three-dimensional FE-based numerical model is developed to analyze the bridge’s structural response under several damage scenarios,including the effects of moving vehicle loads.In particular,the longitudinal and transversal beams are modeled through solid finite elements,while horizontal slabs are made of shell elements.Damage phenomena are also incorporated in the numerical model according to a smeared approach consistent with Continuum Damage Mechanics(CDM).In such a context,the proposed method utilizes an advanced and efficient computational strategy for reproducing Vehicle-Bridge Interaction(VBI)effects based on a moving mesh technique consistent with the Arbitrary Lagrangian-Eulerian(ALE)formulation.The proposed model adopts a moving mesh interface for tracing the positions of the contact points between the vehicle’s wheels and the bridge slabs.Such modeling strategy avoids using extremely refined discretization for structural members,thus drastically reducing computational efforts.Vibrational analyses in terms of damage scenarios are presented to verify how the presence of damage affects the natural frequencies of the structural system.In addition,a comprehensive investigation regarding the response of the bridge under moving vehicles is developed,also providing results in terms of Dynamic Amplification Factor(DAFs)for typical design bridge variables.展开更多
In the service period,the instability of ballastless track bed are mostly related to the damage of interlayers which are mainly resulted from the incompatible thermal deformation of interlayers.The temperature field w...In the service period,the instability of ballastless track bed are mostly related to the damage of interlayers which are mainly resulted from the incompatible thermal deformation of interlayers.The temperature field within the ballastless track bed shows significant non-uniformity due to the large difference in the materials of various structure layers,leading to a considerable difference in the force bearing of different structure layers.Unit Ballastless Track Bed(UBTB)is most significantly affected by temperature gradient.The thermal deformation of interlayers within UBTB follows the trend of ellipsoid-shape buckling under the effect of the temperature gradient,resulting in a variation of the contact relationship between structure layers and a significant periodic irregularity on the rail.When the train travels on the periodically irregular rail,the structure layers are locally contacted,and the contact zone moves with the variation of the wheel position.This wheel-followed local contact greatly magnifies the interlayer stress,causes interlayer damage,and leads to a considerable increase in the bending moment of the track slab.Continuous Ballastless Track Bed(CBTB)is most significantly affected by the overall temperature variation,which may cause damage to the joint in CBTB.Under the combined action of the overall temperature rise and the temperature gradient,the interlayer damage continuously expands,resulting in bonding failure between structural layers.The thermal force in the continuous track slabs will cause the up-heave buckling and the sudden large deformation of the track slab,and the loss of constraint boundary of the horizontal stability.For the design of a ballastless track structure,the change of bearing status and structural damage related to the incompatible thermal deformation of interlayers should be considered.展开更多
Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a p...Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease.However,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche.Here,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells.These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in vitro.Importantly,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts.Overall,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.展开更多
Three treatments including mechanical damage, Lymantria dispar attacking and daubing oral secretions of the in-sects on mechanically damaged cut were conducted on Populus simonii譖opulus pyramibalis c.v. in order to f...Three treatments including mechanical damage, Lymantria dispar attacking and daubing oral secretions of the in-sects on mechanically damaged cut were conducted on Populus simonii譖opulus pyramibalis c.v. in order to find the genuine reason leading to effective resistance response of tree to insects attacking. The release situation of the induced volatiles of the plant was analyzed by TCT-GC/MS at 24 hours after damages. The results indicated that some of the volatiles such as (Z)-3-hexenyl acetate, decanal, 3-hexenyl isovalerate, nonanal, ocimene, and 2-cyanobutane can be induced by both insects attack-ing and mechanical damage, while 2,6-dimethyl-1,3,5,7-octatetraene, 2-methyl-6-methylene-1,7-octadien-3-one, caryophyllene, Isovaleronitrile, diethyl-methyl-benzamide, and dicapryl phthalate were only induced by insects attacking. Such difference in volatiles was attributed to that there existed active components in oral sections of the larvae of Lymantria dispar展开更多
基金This research was financially supported by the Scientific and technological research projects in Sichuan province(Grant Nos.2022YFSY0007 and 2021YFH0010)the National Scientific Science Foundation of China(Grant No.U20A20266).
文摘To investigate the specific creep behavior of ultra-deep buried salt during oil and gas exploitation,a set of triaxial creep experiments was conducted at elevated temperatures with constant axial pressure and unloading confining pressure conditions.Experimental results show that the salt sample deforms more significantly with the increase of applied temperature and deviatoric loading.The accelerated creep phase is not occurring until the applied temperature reaches 130℃,and higher temperature is beneficial to the occurrence of accelerated creep.To describe the specific creep behavior,a novel three-dimensional(3D)creep constitutive model is developed that incorporates the thermal and mechanical variables into mechanical elements.Subsequently,the standard particle swarm optimization(SPSO)method is adopted to fit the experimental data,and the sensibility of key model parameters is analyzed to further illustrate the model function.As a result,the model can accurately predict the creep behavior of salt under the coupled thermo-mechanical effect in deep-buried condition.Based on the research results,the creep mechanical behavior of wellbore shrinkage is predicted in deep drilling projects crossing salt layer,which has practical implications for deep rock mechanics problems.
文摘The changes of hydrogen peroxide (H2O2) metabolism and antioxidant enzyme activities in a hybrid poplar (Populus simonii xp. pyramidalis 'Opera 8277') in response to rnechanical damage (MD) and herbivore wounding (HW) were investigated to determine whether H2O2 could function as the secondary messenger in the signaling of systemic resistance. Results show that H2O2 was generated in wounded leaves through MD and HW treatments and systemically in unwounded leaves around the wounded leaves. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were also enhanced. However, the H2O2 accumulation and antioxidant enzyme activities were inhibited in MD leaves through the pretreatment with DPI (which is a specific inhibitor of NADPH oxidase). The results of this study suggest that H2O2 could be systemically induced by MD and HW treatments, and H2O2 metabolism was closely related to the change in SOD, APX and CAT activities. A high level of antioxidant enzymes could decrease membrane lipid peroxidation levels and effectively induce plant defense responses.
基金Projects(52225403,U2013603,42377143)supported by the National Natural Science Foundation of ChinaProject(2023NSFSC0004)supported by the Sichuan Science and Technology Program,China+1 种基金Project(2023YFB2390200)supported by the National Key R&D Program-Young Scientist Program,ChinaProject(RCJC20210706091948015)supported by the Shenzhen Science Foundation for Distinguished Young Scholars,China。
文摘Laser technology holds significant promise for enhancing rock-breaking efficiency.Experimental investigations were carried out on sandstone subjected to laser radiation,aiming to elucidate its response mechanism to such radiation.The uniaxial compressive strength of sandstone notably decreases by 22.1%–54.7%following exposure to a 750 W laser for 30 s,indicating a substantial weakening effect.Furthermore,the elastic modulus and Poisson ratio of sandstone exhibit an average decrease of 33.7%and 25.9%,respectively.Simultaneously,laser radiation reduces the brittleness of sandstone,increases the dissipated energy proportion,and shifts the failure mode from tensile to tension-shear composite failure.Following laser radiation,both the number and energy of acoustic emission events in the sandstone register a substantial increase,with a more dispersed distribution of these events.In summary,laser radiation induces notable damage to the mechanical properties of sandstone,leading to a substantial decrease in elastic energy storage capacity.Laser rock breaking technology is expected to be applied in hard rock breaking engineering to significantly reduce the difficulty of rock breaking and improve rock breaking efficiency.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.42077231 and 51574156).
文摘This study aims to investigate the mechanical response and acoustic emission(AE)characteristic of pre-flawed sandstone under both monotonic and multilevel constant-amplitude cyclic loads.Specifically,we explored how coplanar flaw angle and load type impact the strength and deformation behavior and microscopic damage mechanism.Results indicated that being fluctuated before rising with increasing fissure angle under monotonic loading,the peak strength of the specimen first increased slowly and then steeply under cyclic loading.The effect of multilevel cyclic loading on the mechanical parameters was more significant.For a single fatigue stage,the specimen underwent greater deformation in early cycles,which subsequently stabilized.Similar variation pattern was also reflected by AE count/energy/b-value.Crack behaviors were dominated by the fissure angle and load type and medium-scale crack accounted for 74.83%–86.44%of total crack.Compared with monotonic loading,crack distribution of specimen under cyclic loading was more complicated.Meanwhile,a simple model was proposed to describe the damage evolution of sandstone under cyclic loading.Finally,SEM images revealed that the microstructures at the fracture were mainly composed of intergranular fracture,and percentage of transgranular fracture jumped under cyclic loading due to the rapid release of elastic energy caused by high loading rate.
基金supported by the National Natural Science Foundation of China (Grant No. 52278420)the China Atomic Energy Authority (CAEA) for China’s URL Development Program and the Geological Disposal Program。
文摘Rock has mechanical characteristics and a fracture damage mechanism that are closely related to its loading history and loading path. The mechanical properties, fracture damage features, acoustic emission(AE) characteristics, and strain energy evolution of the Beishan shallow-layer granite used in triaxial unloading tests were investigated in this study. Three groups of triaxial tests, namely, conventional triaxial compression test(Group Ⅰ), maintaining deviatoric stress synchronously unloading confining pressure test(Group Ⅱ), and loading axial pressure synchronously unloading confining pressure test(Group Ⅲ), were carried out for the cylindrical granite specimens. AE monitoring device was utilized in these tests to determine the degree to which the AE waves and AE events reflected the degree of rock damage. In addition, the crack stress thresholds of the specimens were determined by volumetric strain method and AE parameter method, and strain energy evolution of the rock was explored in different damage stages. The results show that the shallow-layer granite experiences brittle failure during the triaxial loading test and unloading test, and the rock has a greater damage degree during the unloading test. The crack stress thresholds of these samples vary greatly between tests, but the threshold ratios of all samples are similar in the same crack damage stage. The Mogi-Coulomb strength criterion can better describe the unloading failure strength of the rock. The evolution of the AE parameter characteristics and strain energy differs between the specimens used in different stress path tests. The dissipative strain energy is the largest in Group Ⅱ and the smallest in Group Ⅰ.
文摘Aiming to analyze the damage mechanism of UTAO from the perspective of meso-mechanical mechanism using discrete element method(DEM),we conducted study of diseases problems of UTAO in several provinces in China,and found that aggregate spalling was one of the main disease types of UTAO.A discrete element model of UTAO pavement structure was constructed to explore the meso-mechanical mechanism of UTAO damage under the influence of layer thickness,gradation,and bonding modulus.The experimental results show that,as the thickness of UTAO decreasing,the maximum value and the mean value of the contact force between all aggregate particles gradually increase,which leads to aggregates more prone to spalling.Compared with OGFC-5 UTAO,AC-5 UTAO presents smaller maximum and average values of all contact forces,and the loading pressure in AC-5 UTAO is fully diffused in the lateral direction.In addition,the increment of pavement modulus strengthens the overall force of aggregate particles inside UTAO,resulting in aggregate particles peeling off more easily.The increase of bonding modulus changes the position where the maximum value of the tangential force appears,whereas has no effect on the normal force.
基金supported by the National Natural Science Foundation of China(Grant Nos.11672097,11772113)。
文摘Ground-based tests are important for studying hypervelocity impact(HVI)damage to spacecraft pressure vessels in the orbital debris environment.We analyzed the damage to composite overwrapped pressure vessels(COPVs)in the HVI tests and classified the damage into non-catastrophic damage and catastrophic damage.We proposed a numerical simulation method to further study non-catastrophic damage and revealed the characteristics and mechanisms of non-catastrophic damage affected by impact conditions and internal pressures.The fragments of the catastrophically damaged COPVs were collected after the tests.The crack distribution and propagation process of the catastrophic ruptures of the COPVs were analyzed.Our findings contribute to understanding the damage characteristics and mechanisms of COPVs by HVIs.
基金supported by the National Natural Science Foundation of China(11872279,12172258,and 11625210).
文摘The incorporation of commercial flame retardants into fiber-reinforced polymer(FRP)composites has been proposed as a potential solution to improve the latter’s poor flame resistance.However,this approach often poses a challenge,as it can adversely affect the mechanical properties of the FRP.Thus,balancing the need for improved flame resistance with the preservation of mechanical integrity remains a complex issue in FRP research.Addressing this critical concern,this study introduces a novel additive system featuring a combination of one-dimensional(1D)hollow tubular structured halloysite nanotubes(HNTs)and two-dimensional(2D)polygonal flake-shaped nano kaolinite(NKN).By employing a 1D/2D hybrid kaolinite nanoclay system,this research aims to simultaneously improve the flame retardancy and mechanical properties.This innovative approach offers several advantages.During combustion and pyrolysis processes,the 1D/2D hybrid kaolinite nanoclay system proves effective in reducing heat release and volatile leaching.Furthermore,the system facilitates the formation of reinforcing skeletons through a crosslinking mechanism during pyrolysis,resulting in the development of a compact char layer.This char layer acts as a protective barrier,enhancing the material’s resistance to heat and flames.In terms of mechanical properties,the multilayered polygonal flake-shaped 2D NKN plays a crucial role by impeding the formation of cracks that typically arise from vulnerable areas,such as adhesive phase particles.Simultaneously,the 1D HNT bridges these cracks within the matrix,ensuring the structural integrity of the composite material.In an optimal scenario,the homogeneously distributed 1D/2D hybrid kaolinite nanoclays exhibit remarkable results,with a 51.0%improvement in mode II fracture toughness(GIIC),indicating increased resistance to crack propagation.In addition,there is a 34.5%reduction in total heat release,signifying improved flame retardancy.This study represents a significant step forward in the field of composite materials.The innovative use of hybrid low-dimensional nanomaterials offers a promising avenue for the development of multifunctional composites.By carefully designing and incorporating these nanoclays,researchers can potentially create a new generation of FRP composites that excel in both flame resistance and mechanical strength.
基金the Bowen Basin Underground Geotechnical Society for funding this project。
文摘Underground rock dynamic disasters are becoming more severe due to the increasing depth of human operations underground.Underground temperature and pressure conditions contribute significantly to these disasters.Therefore,it is important to understand the coupled thermo-mechanical(TM)behaviour of rocks for the long-term safety and maintenance of underground tunnelling and mining.Moreover,investigation of the damage,strength and failure characteristics of rocks under triaxial stress conditions is important to avoid underground rock disasters.In this study,based on Weibull distribution and Lemaitre's strain equivalent principle,a statistical coupled TM constitutive model for sandstone was established under high temperature and pressure conditions.The triaxial test results of sandstone under different temperature and pressure conditions were used to validate the model.The proposed model was in good agreement with the experimental results up to 600℃.The total TM damage was decreased with increasing temperature,while it was increased with increasing confining pressure.The model's parameters can be calculated using conventional laboratory test results.
基金the National Natural Science Foundation of China (Nos.51323004 and 51574223)the Postdoctoral Science Foundation of China (No.2015M571842)the Open Research Fund of Research Center of Jiangsu Collaborative Innovation Center for Building Energy Saving and Construction Technology (No.SJXTY1502)
文摘To study the mechanical and damage evolution properties of sandstone under triaxial compression, we analyzed the stress strain curve characteristics, deformation and strength properties, and failure process and characteristics of sandstone samples under different stress states. The experimental results reveal that peak strength, residual strength, elasticity modulus and deformation modulus increase linearly with confining pressure, and failure models transform from fragile failure under low confining pressure to ductility failure under high confining pressure. Macroscopic failure forms of samples under uniaxial compression were split failure parallel to the axis of samples, while macroscopic failure forms under uniaxial compression were shear failure, the shear failure angle of which decreased linearly with confin- ing pressure. There were significant volume dilatation properties in the loading process of sandstone under different confining pressures, and we analyzed the damage evolution properties of samples based on acoustic emission damage and volumetric dilatation damage, and established damage constitutive model, realizing the real-time Quantitative evaluation of samnles damage state in loading process.
基金the Fundamental Research Funds for the Central Universities(Project No.2022CDJKYJH037)the National Key R&D Program of China(Grant No.2021YFB3901402)。
文摘To study the damage mechanisms of anhydrite rock under freeze-thaw cycles, the physicalmechanical properties and the microcracking activities of anhydrite rock were investigated through mass variation, nuclear magnetic resonance, scanning electron microscope tests, and uniaxial compression combined with acoustic emission(AE) tests. Results show that with the increase of freeze-thaw processes,the mass, uniaxial compression strength, and elastic modulus of the anhydrite specimens decrease while the porosity and plasticity characteristics increase.For example, after 120 cycles, the uniaxial compression strength and elastic modulus decrease by 46.54% and 60.16%, and the porosity increase by 75%. Combined with the evolution trend of stressstrain curves and the detected events, three stages were labeled to investigate the AE characteristics in freeze-thaw weathered anhydrite rock. It is found that with the increase of freeze-thaw cycles, the proportions of AE counts in stage Ⅰ and stage Ⅱ show a decaying exponential trend. Contrarily, the proportion of AE counts in stage Ⅲ displays an exponential ascending trend. Meanwhile, as the freeze-thaw cycles increase, the low-frequency AE signals increase while the intermediate-frequency AE signals decrease. After 120 cycles, the proportion of low-frequency AE signals increases by 168.95%, and the proportion of intermediate-frequency AE signals reduces by 81.14%. It is concluded that the microtensile cracking events occupy a dominant position during the loading process. With the increase of freeze-thaw cycles, the b value of samples decreases.After 120 cycles, b value decreases by 27.2%, which means that the proportion of cracking events in rocks with small amplitude decreases. Finally, it is proposed that the freeze-thaw damage mechanism of anhydrite is also characterized by the water chemical softening effect.
基金Science and technology development project of China Railway Ninth Bureau Group Co.,Ltd,Grant/Award Number:DLF‐ML‐JSFW‐2021‐09Science and Technology Development Project of China Railway Research Institute Co.Ltd,Grant/Award Number:2017‐KJ008‐Z008‐XB+2 种基金Gansu Province Youth Science and Technology Fund program,China,Grant/Award Number:21JR7RA739Natural Science Foundation of Gansu Province,China,Grant/Award Number:21JR7RA738National Key R&D Program of China,Grant/Award Number:2018YFC1504901。
文摘To study the damage mechanism of multi‐anchor piles in tunnel crossing landslide area under earthquake,the damping performance of multi‐anchor piles was discussed.The energy dissipation springs were used as the optimization device of the anchor head to carry out the shaking table comparison test on the reinforced slope.The Hilbert spectrum and Hilbert marginal spectrum were proposed to analyze the seismic damage mechanism of the multi‐anchor piles,and the peak Fourier spectrum amplitude(PFSA)was used to verify the effectiveness of the method.The results show that the seismic energy is concentrated in the high‐frequency component(30-40Hz)of the Hilbert spectrum and the low‐frequency component(12-30 Hz)of the marginal spectrum.This indicates that they can be combined with the distribution law of the PFSA to identify the overall and local dynamic responses of the multi‐anchored piles,respectively.The stretchable deformation of the energy‐dissipation springs improves the coordination of the multi‐anchor piles,resulting in better pile integrity.The damage mechanism of the multi‐anchor piles is elucidated based on the energy method:local damage at the top and middle areas of the multi‐anchor piles is mainly caused by the low‐frequency component(12-30 Hz)of the marginal spectrum under the action of 0.15g and 0.20g seismic intensities.As the seismic intensity increases to 0.30g,the dynamic response of the slope is further amplified by the high‐frequency component(30-40 Hz)of the Hilbert energy spectrum,which leads to the overall damage of the multi‐anchor piles.
基金Supported by the National Key Technologies Research&Development Program(2017YFC0804607)the National Key Basic Research Development Plan(973 Proect)(2014CB047000)
文摘By using the technique of the split Hopkinson pressure bar( SHPB),impact tests at different stress wavelengths( 0. 8-2. 0 m) and strain rates( 20-120 s^(-1)) were conducted to study the dynamic mechanical properties and damage accumulation evolution lawof granite. Test results showthat the dynamic compressive strength and strain rate of granite have a significantly exponential correlation;the relationship between peak strain and strain rate is approximately linear,and the increase of wavelengths generally makes the level of peak strain uplift. The multiple-impacts test at a lowstrain rate indicates that at the same wavelength,the cumulative damage of granite shows an exponential increasing form with the increase of strain rate; when keeping the increase of strain rate constant and increasing the stress wavelength,the damage accumulation effect of granite is intensified and still shows an exponential increasing form; under the effect of multiple impacts,the damage development trend of granite is similar overall,but the increase rate is accelerating. Therefore the damage evolution model was established on the basis of the exponential function while the physical meaning of parameters in the model was determined. The model can reflect the effect of the wave parameters and multiple impacts. The validity of the model and the physical meaning of the parameters were verified by the test,which further offer a reference for correlational research and engineering application for the granite.
文摘The process of thermal stress damage during 1080 nm laser ablation of single-crystal germanium was recorded in real time using a high-speed charge-coupled device.A three-dimensional finite element numerical model based on Fourier's heat conduction equation,Hooke's law and the Alexander–Hasson equation was developed to analyze the thermal stress damage mechanism involved.The damage morphology of the ablated samples was observed using an optical microscope.The results show that the cooling process has an important influence on fracture in the laser-irradiated region of single-crystal germanium.Fracture is the result of a combination of thermal stress and reduction in local yield strength.
基金support from the National Natural Science Foundation of China(Grant No.52208387)Open Fund of Key Laboratory of Geohazard Prevention of Hilly Mountains,Ministry of Land and Resources,China(Fujian Key Laboratory of Geohazard Prevention)(Grant No.FJKLGH2022K001).
文摘Full-length grouted bolts play a crucial role in geotechnical engineering thanks to their excellent stability.However,few studies have been concerned with the degrading performance of grouted rock bolts caused by extensive and continuous heat conduction from surrounding rocks in high-geothermal tunnels buried more than 100 m(temperature from 28C to 100C).To investigate the damage mechanism,we examined the time-varying behaviors of grouted rock bolts in both constant and variable temperature curing environments and their damage due to the coupling effects of high temperature and humidity through mechanical and micro-feature tests,including uniaxial compression test,pull-out test,computed tomography(CT)scans,X-ray diffraction(XRD)test,thermogravimetric analysis(TGA),etc.,and further analyzed the relationship between grout properties and anchorage capability.In order to facilitate a rapid assessment and control of the anchorage performance of anchors in different conditions,results of the interface bond degradation tests were correlated to environment parameters based on the damage model of interfacial bond stress proposed.Accordingly,a thermal hazard classification criterion for anchorage design in high-geothermal tunnels was suggested.Based on the reported results,although high temperature accelerated the early-stage hydration reaction of grouting materials,it affected the distribution and quantity of hydration products by inhibiting hydration degree,thus causing mechanical damage to the anchorage system.There was a significant positive correlation between the strength of the grouting material and the anchoring force.Influenced by the changes in grout properties,three failure patterns of rock bolts typically existed.Applying a hot-wet curing regime results in less reduction in anchorage force compared to the hot-dry curing conditions.The findings of this study would contribute to the design and investigations of grouted rock bolts in high-geothermal tunnels.
基金funded by Open Fund of State Key Laboratory of Water Resource Protection and Utilization in Coal Mining (GJNY-20-113-03),SHGF-16-19the Fundamental Research Funds for the Central Universities (06500182)+2 种基金Funds from Joint National-Local Engineering Research Center for Safe and Precise Coal Mining (EC2021004)Funds from State Key Laboratory of Coal Resources in Western China (SKLCRKF20-07)Funds from Humboldt Research Fellowship,Funds from NSFC (52204086).
文摘The stability of coal walls(pillars)can be seriously undermined by diverse in-situ dynamic disturbances.Based on a 3D par-ticle model,this work strives to numerically replicate the major mechanical responses and acoustic emission(AE)behaviors of coal samples under multi-stage compressive cyclic loading with different loading and unloading rates,which is termed differential cyclic loading(DCL).A Weibull-distribution-based model with heterogeneous bond strengths is constructed by both considering the stress-strain relations and AE parameters.Six previously loaded samples were respectively grouped to indicate two DCL regimes,the damage mechanisms for the two groups are explicitly characterized via the time-stress-dependent variation of bond size multiplier,and it is found the two regimes correlate with distinct damage patterns,which involves the competition between stiffness hardening and softening.The numerical b-value is calculated based on the mag-nitudes of AE energy,the results show that both stress level and bond radius multiplier can impact the numerical b-value.The proposed numerical model succeeds in replicating the stress-strain relations of lab data as well as the elastic-after effect in DCL tests.The effect of damping on energy dissipation and phase shift in numerical model is summarized.
基金supported by Ministry of University and Research(MUR)through the Research Grant“PRIN 2020 No.2020EBLPLS”“Programma Operativo Nazionale(PON)2014-2020”.
文摘This work proposes a numerical investigation on the effects of damage on the structural response of Reinforced Concrete(RC)bridge structures commonly adopted in highway and railway networks.An effective three-dimensional FE-based numerical model is developed to analyze the bridge’s structural response under several damage scenarios,including the effects of moving vehicle loads.In particular,the longitudinal and transversal beams are modeled through solid finite elements,while horizontal slabs are made of shell elements.Damage phenomena are also incorporated in the numerical model according to a smeared approach consistent with Continuum Damage Mechanics(CDM).In such a context,the proposed method utilizes an advanced and efficient computational strategy for reproducing Vehicle-Bridge Interaction(VBI)effects based on a moving mesh technique consistent with the Arbitrary Lagrangian-Eulerian(ALE)formulation.The proposed model adopts a moving mesh interface for tracing the positions of the contact points between the vehicle’s wheels and the bridge slabs.Such modeling strategy avoids using extremely refined discretization for structural members,thus drastically reducing computational efforts.Vibrational analyses in terms of damage scenarios are presented to verify how the presence of damage affects the natural frequencies of the structural system.In addition,a comprehensive investigation regarding the response of the bridge under moving vehicles is developed,also providing results in terms of Dynamic Amplification Factor(DAFs)for typical design bridge variables.
基金supported by the National Natural Science Foundation of China(grant numbers 52278466).
文摘In the service period,the instability of ballastless track bed are mostly related to the damage of interlayers which are mainly resulted from the incompatible thermal deformation of interlayers.The temperature field within the ballastless track bed shows significant non-uniformity due to the large difference in the materials of various structure layers,leading to a considerable difference in the force bearing of different structure layers.Unit Ballastless Track Bed(UBTB)is most significantly affected by temperature gradient.The thermal deformation of interlayers within UBTB follows the trend of ellipsoid-shape buckling under the effect of the temperature gradient,resulting in a variation of the contact relationship between structure layers and a significant periodic irregularity on the rail.When the train travels on the periodically irregular rail,the structure layers are locally contacted,and the contact zone moves with the variation of the wheel position.This wheel-followed local contact greatly magnifies the interlayer stress,causes interlayer damage,and leads to a considerable increase in the bending moment of the track slab.Continuous Ballastless Track Bed(CBTB)is most significantly affected by the overall temperature variation,which may cause damage to the joint in CBTB.Under the combined action of the overall temperature rise and the temperature gradient,the interlayer damage continuously expands,resulting in bonding failure between structural layers.The thermal force in the continuous track slabs will cause the up-heave buckling and the sudden large deformation of the track slab,and the loss of constraint boundary of the horizontal stability.For the design of a ballastless track structure,the change of bearing status and structural damage related to the incompatible thermal deformation of interlayers should be considered.
基金supported by the National Key Research and Development Program of China,Nos.2017YFE0122900(to BH),2019YFA0110800(to WL),2019YFA0903802(to YW),2021YFA1101604(to LW),2018YFA0108502(to LF),and 2020YFA0804003(to JW)the National Natural Science Foundation of China,Nos.31621004(to WL,BH)and 31970821(to YW)+1 种基金CAS Project for Young Scientists in Basic Research,No.YSBR-041(to YW)Joint Funds of the National Natural Science Foundation of China,No.U21A20396(to BH)。
文摘Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease.However,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche.Here,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells.These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in vitro.Importantly,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts.Overall,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.
基金This research is supported by National Natural Science Foundation of China (No.30170764) and the Postgraduate Training Fund of Graduate School of Beijing Forestry University (No.03SW004)
文摘Three treatments including mechanical damage, Lymantria dispar attacking and daubing oral secretions of the in-sects on mechanically damaged cut were conducted on Populus simonii譖opulus pyramibalis c.v. in order to find the genuine reason leading to effective resistance response of tree to insects attacking. The release situation of the induced volatiles of the plant was analyzed by TCT-GC/MS at 24 hours after damages. The results indicated that some of the volatiles such as (Z)-3-hexenyl acetate, decanal, 3-hexenyl isovalerate, nonanal, ocimene, and 2-cyanobutane can be induced by both insects attack-ing and mechanical damage, while 2,6-dimethyl-1,3,5,7-octatetraene, 2-methyl-6-methylene-1,7-octadien-3-one, caryophyllene, Isovaleronitrile, diethyl-methyl-benzamide, and dicapryl phthalate were only induced by insects attacking. Such difference in volatiles was attributed to that there existed active components in oral sections of the larvae of Lymantria dispar