Kidney transplantation is one of the important means in saving the life of patients with renal diseases of the terminal stage. However, rejection reaction often results in the loss of the grafted organs’ function. Al...Kidney transplantation is one of the important means in saving the life of patients with renal diseases of the terminal stage. However, rejection reaction often results in the loss of the grafted organs’ function. Almost half the recipients have been found to suffer from loss of graft function due to rejection reaction within 10 years of post-transplantation. Part of them have bad quality of life展开更多
Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of new technology of integrated Chinese and western medicine...Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of new technology of integrated Chinese and western medicine in treating sequelae of cerebrovascular diseases is planned to be held in Nanning,Guangxi in August.展开更多
Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of TCM classic prescriptions and herbs in the treatment of e...Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of TCM classic prescriptions and herbs in the treatment of emotional diseaseis planned to be held in Yichang,Hubei province from June 22nd to 24th.展开更多
[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control gr...[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control group,model group,Jipei Dilong Ointment high,medium and low dose groups,and positive drug Diclofenac group,with 12 rats in each group.Except the control group,all other groups were injected with 0.2 mL of 2%papain and 0.3%L-cysteine mixture into knee joint cavity to establish osteoarthritis model,while the control group was injected with the same amount of normal saline.The Lequesne MG score was used to determine the success of the model.After successful modeling,external administration was given for 4 weeks.The histopathological changes in articular cartilage and synovium were observed by HE staining;the levels of TNF-α,IL-1βand COX-2 in serum were detected by ELISA,and the relative expression of MMP-9 and TIMP-1 mRNA in cartilage was detected by qRT-PCR;the relative expression of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB in rat cartilage was detected by Western-blot.[Results]Compared with the model group,Jipei Dilong Ointment could significantly reduce the Lequesne MG score and Mankin s score of arthritic rats(P<0.01);significantly improve the pathological changes in articular cartilage and synovium,reduce tissue edema,necrosis,inflammatory cell infiltration and fibrous tissue proliferation;reduce the expression of MMP-9 mRNA(P<0.01)in different degrees and increase the expression of TIMP-1 mRNA(P<0.01);reduce the relative expression of MMP-9,TLR4,MyD88 and NF-κB protein in different degrees(P<0.01),and significantly increase the relative expression of TIMP-1 protein in cartilage(P<0.01).[Conclusions]Jipei Dilong Ointment can improve the joint injury of osteoarthritis rats,and the mechanism may be related to the regulation of TLR4/MyD88/NF-κB signaling pathway and the ratio of MMP-9/TIMP-1.展开更多
Medicinal plants are popular and widely used as a major source of herbal drugs and pharmaceutical compounds. Ever-growing demands make medicinal plants faced to several problems including efficacy and safety to meet b...Medicinal plants are popular and widely used as a major source of herbal drugs and pharmaceutical compounds. Ever-growing demands make medicinal plants faced to several problems including efficacy and safety to meet business requirements, conservation, and artificially assisted breeding. As a powerful molecular tool, microsatellites offer the great potentials for various purposes in plants. This review provides a scenario of microsatellites in medicinal plants including development from genomic or expressed sequence tag libraries, cross-species transferability, genotyping, and potential applications. We emphasized on the authentication of medicinal plants by microsatellite markers.展开更多
Exactly 30 years ago,Nakata and coworkers published a paper analyzing the 1664-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions in Escherichia coli（Ishino et al.,1...Exactly 30 years ago,Nakata and coworkers published a paper analyzing the 1664-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions in Escherichia coli（Ishino et al.,1987）.展开更多
In order to understand the fundamental questions of the biology of life and to duplicate the pathogenesis of human diseases, animal models using different experimental animals, such as rodents, Drosophila, Caenorhabdi...In order to understand the fundamental questions of the biology of life and to duplicate the pathogenesis of human diseases, animal models using different experimental animals, such as rodents, Drosophila, Caenorhabditis elegans, and zebrafish, have been established and used widely for many decades. The controllability of environmental conditions, the high reproducibility, the ease of scale and the comparability of results, as well as the ability to use different standards for ethical protocols, all make an animal model the ideal tool for carrying out studies on human diseases and the development of novel pharmaceuticals and new therapies （Xue et al., 2014）. An ideal animal model should reflect the complete spectra of a specific human disease, with similar features on the following key issues： （1） genetic basis; （2） anatomy and physiology; （3） pathological response（s） and underlying mechanism（s）; （4） phenotypic endpoints as clinical studies; （5） responsiveness to known drugs with clinical efficacy; and （6） prediction of clinical efficacy （McGonigle and Ruggeri, 2014）.展开更多
Traditional Chinese Medicine(TCM)has been used in prevention and treatment of disease in clinical practice for thousands of years,with an indispensable role of multiple ingredients.Thus,a rapid and effective chemical ...Traditional Chinese Medicine(TCM)has been used in prevention and treatment of disease in clinical practice for thousands of years,with an indispensable role of multiple ingredients.Thus,a rapid and effective chemical ingredients analysis was of necessary to be established for the evaluation of the holistic quality of TCM.As could afford the data with high resolution and high sensitivity,展开更多
One hundred forty-four patients of cancer pain were treated with external application of QuTong Ling, and the effect of pain remission was observed. The total remission rate was 79. 2% with themaintaining time of 6. 4...One hundred forty-four patients of cancer pain were treated with external application of QuTong Ling, and the effect of pain remission was observed. The total remission rate was 79. 2% with themaintaining time of 6. 42 1 . 24 hours, proving it was superior to butycinnamyl pyrazirum.展开更多
文摘Kidney transplantation is one of the important means in saving the life of patients with renal diseases of the terminal stage. However, rejection reaction often results in the loss of the grafted organs’ function. Almost half the recipients have been found to suffer from loss of graft function due to rejection reaction within 10 years of post-transplantation. Part of them have bad quality of life
文摘Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of new technology of integrated Chinese and western medicine in treating sequelae of cerebrovascular diseases is planned to be held in Nanning,Guangxi in August.
文摘Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of TCM classic prescriptions and herbs in the treatment of emotional diseaseis planned to be held in Yichang,Hubei province from June 22nd to 24th.
基金Supported by National Key R&D Program(2019YFC1712500)Science and Technology Program of Guizhou Province([2020]3003)Project of Guizhou University of Traditional Chinese Medicine(2018YFC170810520).
文摘[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control group,model group,Jipei Dilong Ointment high,medium and low dose groups,and positive drug Diclofenac group,with 12 rats in each group.Except the control group,all other groups were injected with 0.2 mL of 2%papain and 0.3%L-cysteine mixture into knee joint cavity to establish osteoarthritis model,while the control group was injected with the same amount of normal saline.The Lequesne MG score was used to determine the success of the model.After successful modeling,external administration was given for 4 weeks.The histopathological changes in articular cartilage and synovium were observed by HE staining;the levels of TNF-α,IL-1βand COX-2 in serum were detected by ELISA,and the relative expression of MMP-9 and TIMP-1 mRNA in cartilage was detected by qRT-PCR;the relative expression of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB in rat cartilage was detected by Western-blot.[Results]Compared with the model group,Jipei Dilong Ointment could significantly reduce the Lequesne MG score and Mankin s score of arthritic rats(P<0.01);significantly improve the pathological changes in articular cartilage and synovium,reduce tissue edema,necrosis,inflammatory cell infiltration and fibrous tissue proliferation;reduce the expression of MMP-9 mRNA(P<0.01)in different degrees and increase the expression of TIMP-1 mRNA(P<0.01);reduce the relative expression of MMP-9,TLR4,MyD88 and NF-κB protein in different degrees(P<0.01),and significantly increase the relative expression of TIMP-1 protein in cartilage(P<0.01).[Conclusions]Jipei Dilong Ointment can improve the joint injury of osteoarthritis rats,and the mechanism may be related to the regulation of TLR4/MyD88/NF-κB signaling pathway and the ratio of MMP-9/TIMP-1.
基金National Natural Science Foundation of China(31270340,31200225,30800624)Instrument Developing Project of the Chinese Academy of Sciences(YZ201227)+1 种基金the Key Research Program of the Chinese Academy of Sciences(Grant NO.KSZD-EW-Z-004)Grant from Zhongning County
文摘Medicinal plants are popular and widely used as a major source of herbal drugs and pharmaceutical compounds. Ever-growing demands make medicinal plants faced to several problems including efficacy and safety to meet business requirements, conservation, and artificially assisted breeding. As a powerful molecular tool, microsatellites offer the great potentials for various purposes in plants. This review provides a scenario of microsatellites in medicinal plants including development from genomic or expressed sequence tag libraries, cross-species transferability, genotyping, and potential applications. We emphasized on the authentication of medicinal plants by microsatellite markers.
文摘Exactly 30 years ago,Nakata and coworkers published a paper analyzing the 1664-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions in Escherichia coli（Ishino et al.,1987）.
文摘In order to understand the fundamental questions of the biology of life and to duplicate the pathogenesis of human diseases, animal models using different experimental animals, such as rodents, Drosophila, Caenorhabditis elegans, and zebrafish, have been established and used widely for many decades. The controllability of environmental conditions, the high reproducibility, the ease of scale and the comparability of results, as well as the ability to use different standards for ethical protocols, all make an animal model the ideal tool for carrying out studies on human diseases and the development of novel pharmaceuticals and new therapies （Xue et al., 2014）. An ideal animal model should reflect the complete spectra of a specific human disease, with similar features on the following key issues： （1） genetic basis; （2） anatomy and physiology; （3） pathological response（s） and underlying mechanism（s）; （4） phenotypic endpoints as clinical studies; （5） responsiveness to known drugs with clinical efficacy; and （6） prediction of clinical efficacy （McGonigle and Ruggeri, 2014）.
文摘Traditional Chinese Medicine(TCM)has been used in prevention and treatment of disease in clinical practice for thousands of years,with an indispensable role of multiple ingredients.Thus,a rapid and effective chemical ingredients analysis was of necessary to be established for the evaluation of the holistic quality of TCM.As could afford the data with high resolution and high sensitivity,
文摘One hundred forty-four patients of cancer pain were treated with external application of QuTong Ling, and the effect of pain remission was observed. The total remission rate was 79. 2% with themaintaining time of 6. 42 1 . 24 hours, proving it was superior to butycinnamyl pyrazirum.
