At the level of in vitro drug screening,the development of a phenotypic analysis system with highcontent screening at the core provides a strong platform to support high-throughput drug screening.There are few systema...At the level of in vitro drug screening,the development of a phenotypic analysis system with highcontent screening at the core provides a strong platform to support high-throughput drug screening.There are few systematic reports on brain organoids,as a new three-dimensional in vitro model,in terms of model stability,key phenotypic fingerprint,and drug screening schemes,and particula rly rega rding the development of screening strategies for massive numbers of traditional Chinese medicine monomers.This paper reviews the development of brain organoids and the advantages of brain organoids over induced neurons or cells in simulated diseases.The paper also highlights the prospects from model stability,induction criteria of brain organoids,and the screening schemes of brain organoids based on the characteristics of brain organoids and the application and development of a high-content screening system.展开更多
According to the last World Malaria Report,there were an estimated 584 000 malaria deaths worldwide in 2013.The problem of parasite resistance towards available medicines is increasing.Natural products could play an i...According to the last World Malaria Report,there were an estimated 584 000 malaria deaths worldwide in 2013.The problem of parasite resistance towards available medicines is increasing.Natural products could play an important role to discover new antimalarial drug.The aim of the study was to highlight the anti-plasmodial properties of plants used in traditional medicine on the Mascarene Islands.85展开更多
The effects of the extracts of 20 Chinese medicinal herbs and an antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. The extracts of P. urinaria showed a ...The effects of the extracts of 20 Chinese medicinal herbs and an antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. The extracts of P. urinaria showed a dose-dependent inhibition on DHBV DNAp. And those of other herbs showed little inhibition effect. Primary duck hepatocyte (PDH) cultures were used for evaluating effects of the extract of P. urinaria, foscarnet and acyclovir (ACV) on DHBV, and all the drugs or the extracts showed inhibition of DHBV DNA replication. Furthermore, in vivo trials were carried out. Peking ducks infected with LJ-76 strain of DHBV were treated with the extract of P. urinaria or ACV and compared with placebo treated control ducks. The treatment results in the loss or reduction of circulating viral DHBV DNA and DHBsAg.展开更多
Malaria,caused by protozoa of the genus Plasmodium,causes some 198 million of confirmed cases and about584,000 of deaths per year.Its management needs both accurate diagnostic and treatments availability.The current d...Malaria,caused by protozoa of the genus Plasmodium,causes some 198 million of confirmed cases and about584,000 of deaths per year.Its management needs both accurate diagnostic and treatments availability.The current diagnostic techniques(rapid diagnostic tests and microscopy)appear either too expensive,lowly sensitive or difficult to implement.The hemozoin('malaria pigment')展开更多
基金supported by the National Natural Science Foundation of China,No.32000498the Startup Funding of Zhejiang University City College,No.210000-581849 (both to CG)National College Students’Innovative Entrepreneurial Training Plan Program,No.2021 13021024 (to JQZ)。
文摘At the level of in vitro drug screening,the development of a phenotypic analysis system with highcontent screening at the core provides a strong platform to support high-throughput drug screening.There are few systematic reports on brain organoids,as a new three-dimensional in vitro model,in terms of model stability,key phenotypic fingerprint,and drug screening schemes,and particula rly rega rding the development of screening strategies for massive numbers of traditional Chinese medicine monomers.This paper reviews the development of brain organoids and the advantages of brain organoids over induced neurons or cells in simulated diseases.The paper also highlights the prospects from model stability,induction criteria of brain organoids,and the screening schemes of brain organoids based on the characteristics of brain organoids and the application and development of a high-content screening system.
文摘According to the last World Malaria Report,there were an estimated 584 000 malaria deaths worldwide in 2013.The problem of parasite resistance towards available medicines is increasing.Natural products could play an important role to discover new antimalarial drug.The aim of the study was to highlight the anti-plasmodial properties of plants used in traditional medicine on the Mascarene Islands.85
文摘The effects of the extracts of 20 Chinese medicinal herbs and an antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. The extracts of P. urinaria showed a dose-dependent inhibition on DHBV DNAp. And those of other herbs showed little inhibition effect. Primary duck hepatocyte (PDH) cultures were used for evaluating effects of the extract of P. urinaria, foscarnet and acyclovir (ACV) on DHBV, and all the drugs or the extracts showed inhibition of DHBV DNA replication. Furthermore, in vivo trials were carried out. Peking ducks infected with LJ-76 strain of DHBV were treated with the extract of P. urinaria or ACV and compared with placebo treated control ducks. The treatment results in the loss or reduction of circulating viral DHBV DNA and DHBsAg.
基金The Walloon Region and the ARES-CCD are gratefully acknowledged for funding(Grants FSO 1217602 and PRD DIABIOMAL)
文摘Malaria,caused by protozoa of the genus Plasmodium,causes some 198 million of confirmed cases and about584,000 of deaths per year.Its management needs both accurate diagnostic and treatments availability.The current diagnostic techniques(rapid diagnostic tests and microscopy)appear either too expensive,lowly sensitive or difficult to implement.The hemozoin('malaria pigment')
