Crosstalk among canonical Wnt and Hippo pathway members in skeletal muscle and at the neuromuscular junction

Skeletal muscles are essential for locomotion,posture,and metabolic regulation.To understand physiological processes,exercise adaptation,and muscle-related disorders,it is critical to understand the molecular pathways that underlie skeletal muscle function.The process of muscle contra ction,orchestrated by a complex interplay of molecular events,is at the core of skeletal muscle function.Muscle contraction is initiated by an action potential and neuromuscular transmission requiring a neuromuscular junction.Within muscle fibers,calcium ions play a critical role in mediating the interaction between actin and myosin filaments that generate force.Regulation of calcium release from the sarcoplasmic reticulum plays a key role in excitation-contraction coupling.The development and growth of skeletal muscle are regulated by a network of molecular pathways collectively known as myogenesis.Myogenic regulators coordinate the diffe rentiation of myoblasts into mature muscle fibers.Signaling pathways regulate muscle protein synthesis and hypertrophy in response to mechanical stimuli and nutrient availability.Seve ral muscle-related diseases,including congenital myasthenic disorders,sarcopenia,muscular dystrophies,and metabolic myopathies,are underpinned by dys regulated molecular pathways in skeletal muscle.Therapeutic interventions aimed at preserving muscle mass and function,enhancing regeneration,and improving metabolic health hold promise by targeting specific molecular pathways.Other molecular signaling pathways in skeletal muscle include the canonical Wnt signaling pathway,a critical regulator of myogenesis,muscle regeneration,and metabolic function,and the Hippo signaling pathway.In recent years,more details have been uncovered about the role of these two pathways during myogenesis and in developing and adult skeletal muscle fibers,and at the neuromuscular junction.In fact,research in the last few years now suggests that these two signaling pathways are interconnected and that they jointly control physiological and pathophysiological processes in muscle fibers.In this review,we will summarize and discuss the data on these two pathways,focusing on their concerted action next to their contribution to skeletal muscle biology.However,an in-depth discussion of the noncanonical Wnt pathway,the fibro/a dipogenic precursors,or the mechanosensory aspects of these pathways is not the focus of this review.

Aldo-keto reductase family member C3(AKR1C3)promotes hepatocellular carcinoma cell growth by producing prostaglandin F2α

Hepatocellular carcinoma(HCC)is a leading cause of death worldwide.Current therapies are effective for HCC patients with early disease,but many patients suffer recurrence after surgery and have a poor response to chemotherapy.Therefore,new therapeutic targets are needed.We analyzed gene expression profiles between HCC tissues and normal adjacent tissues from public databases and found that the expression of genes involved in lipid metabolism was significantly different.The analysis showed that AKR1C3 was upregulated in tumors,and high AKR1C3 expression was associated with a poorer prognosis in HCC patients.In vitro,assays demonstrated that the knockdown of AKR1C3 or the addition of the AKR1C3 inhibitor indomethacin suppressed the growth and colony formation of HCC cell lines.Knockdown of AKR1C3 in Huh7 cells reduced tumor growth in vivo.To explore the mechanism,we performed pathway enrichment analysis,and the results linked the expression of AKR1C3 with prostaglandin F2 alpha(PGF2a)downstream target genes.Suppression of AKR1C3 activity reduced the production of PGF2a,and supplementation with PGF2a restored the growth of indomethacin-treated Huh7 cells.Knockdown of the PGF receptor(PTGFR)and treatment with a PTGFR inhibitor significantly reduced HCC growth.We showed that indomethacin potentiated the sensitivity of Huh7 cells to sorafenib.In summary,our results indicate that AKR1C3 upregulation may promote HCC growth by promoting the production of PGF2α,and suppression of PTGFR limited HCC growth.Therefore,targeting the AKR1C3-PGF2a-PTGFR axis may be a new strategy for the treatment of HCC.

Characteristics and control factors of feldspar dissolution in gravity flow sandstone of Chang 7 Member,Triassic Yanchang Formation,Ordos Basin,NW China

To clarify the formation and distribution of feldspar dissolution pores and predict the distribution of high-quality reservoir in gravity flow sandstone of the 7^(th) member of Triassic Yanchang Formation(Chang 7 Member)in the Ordos Basin,thin sections,scanning electron microscopy,energy spectrum analysis,X-ray diffraction whole rock analysis,and dissolution experiments are employed in this study to investigate the characteristics and control factors of feldspar dissolution pores.The results show that:(1)Three types of diagenetic processes are observed in the feldspar of Chang 7 sandstone in the study area:secondary overgrowth of feldspar,replacement by clay and calcite,and dissolution of detrital feldspar.(2)The feldspar dissolution of Chang 7 tight sandstone is caused by organic acid,and is further affected by the type of feldspar,the degree of early feldspar alteration,and the buffering effect of mica debris on organic acid.(3)Feldspars have varying degrees of dissolution.Potassium feldspar is more susceptible to dissolution than plagioclase.Among potassium feldspar,orthoclase is more soluble than microcline,and unaltered feldspar is more soluble than early kaolinized or sericitized feldspar.(4)The dissolution experiment demonstrated that the presence of mica can hinder the dissolution of feldspar.Mica of the same mass has a significantly stronger capacity to consume organic acids than feldspar.(5)Dissolution pores in feldspar of Chang 7 Member are more abundant in areas with low mica content,and they improve the reservoir physical properties,while in areas with high mica content,the number of feldspar dissolution pores decreases significantly.

Tanshinone ⅡA improves Alzheimer’s disease via RNA nuclearenriched abundant transcript 1/microRNA-291a-3p/member RAS oncogene family Rab22a axis

BACKGROUND Alzheimer’s disease(AD)is a neurodegenerative condition characterized by oxidative stress and neuroinflammation.Tanshinone ⅡA(Tan-ⅡA),a bioactive compound isolated from Salvia miltiorrhiza plants,has shown potential neuroprotective effects;however,the mechanisms underlying such a function remain unclear.AIM To investigate potential Tan-ⅡA neuroprotective effects in AD and to elucidate their underlying mechanisms.METHODS Hematoxylin and eosin staining was utilized to analyze structural brain tissue morphology.To assess changes in oxidative stress and neuroinflammation,we performed enzyme-linked immunosorbent assay and western blotting.Additionally,the effect of Tan-ⅡA on AD cell models was evaluated in vitro using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Genetic changes related to the long non-coding RNA(lncRNA)nuclear-enriched abundant transcript 1(NEAT1)/microRNA(miRNA,miR)-291a-3p/member RAS oncogene family Rab22a axis were assessed through reverse transcription quantitative polymerase chain reaction.RESULTS In vivo,Tan-ⅡA treatment improved neuronal morphology and attenuated oxidative stress and neuroinflammation in the brain tissue of AD mice.In vitro experiments showed that Tan-ⅡA dose-dependently ameliorated the amyloid-beta 1-42-induced reduction of neural stem cell viability,apoptosis,oxidative stress,and neuroinflammation.In this process,the lncRNA NEAT1-a potential therapeutic target-is highly expressed in AD mice and downregulated via Tan-ⅡA treatment.Mechanistically,NEAT1 promotes the transcription and translation of Rab22a via miR-291a-3p,which activates nuclear factor kappa-B(NF-κB)signaling,leading to activation of the pro-apoptotic B-cell lymphoma 2-associated X protein and inhibition of the anti-apoptotic B-cell lymphoma 2 protein,which exacerbates AD.Tan-ⅡA intervention effectively blocked this process by inhibiting the NEAT1/miR-291a-3p/Rab22a axis and NF-κB signaling.CONCLUSION This study demonstrates that Tan-ⅡA exerts neuroprotective effects in AD by modulating the NEAT1/miR-291a-3p/Rab22a/NF-κB signaling pathway,serving as a foundation for the development of innovative approaches for AD therapy.

MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1-extracellular signal-regulated kinase-1/2 axis

BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer physiology.However,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC).AIM To investigate the role and molecular mechanism of miRNA-145-5p(miR145-5p)in the progression of GC.METHODS Real-time polymerase chain reaction(RT-PCR)was used to detect miRNA expression in human GC tissues and cells.The ability of cancer cells to migrate and invade was assessed using wound-healing and transwell assays,respectively.Cell proliferation was measured using cell counting kit-8 and colony formation assays,and apoptosis was evaluated using flow cytometry.Expression of the epithelial-mesenchymal transition(EMT)-associated protein was determined by Western blot.Targets of miR-145-5p were predicated using bioinformatics analysis and verified using a dual-luciferase reporter system.Serpin family E member 1(SERPINE1)expression in GC tissues and cells was evaluated using RT-PCR and immunohistochemical staining.The correlation between SERPINE1 expression and overall patient survival was determined using Kaplan-Meier plot analysis.The association between SERPINE1 and GC progression was also tested.A rescue experiment of SERPINE1 overexpression was conducted to verify the relationship between this protein and miR-145-5p.The mechanism by which miR-145-5p influences GC progression was further explored by assessing tumor formation in nude mice.RESULTS GC tissues and cells had reduced miR-145-5p expression and SERPINE1 was identified as a direct target of this miRNA.Overexpression of miR-145-5p was associated with decreased GC cell proliferation,invasion,migration,and EMT,and these effects were reversed by forcing SERPINE1 expression.Kaplan-Meier plot analysis revealed that patients with higher SERPINE1 expression had a shorter survival rate than those with lower SERPINE1 expression.Nude mouse tumorigenesis experiments confirmed that miR-145-5p targets SERPINE1 to regulate extracellular signal-regulated kinase-1/2(ERK1/2).CONCLUSION This study found that miR-145-5p inhibits tumor progression and is expressed in lower amounts in patients with GC.MiR-145-5p was found to affect GC cell proliferation,migration,and invasion by negatively regulating SERPINE1 levels and controlling the ERK1/2 pathway.

Mutations in Ras homolog family member A in patients with peripheral T-cell lymphoma and implications for personalized medicine

Genome sequencing has revealed frequent mutations in Ras homolog family member A(RHOA)among various cancers with unique aberrant profiles and pathogenic effects,especially in peripheral T-cell lymphoma(PTCL).The discrete positional distribution and types of RHOA amino acid substitutions vary according to the tumor type,thereby leading to different functional and biological properties,which provide new insight into the molecular pathogenesis and potential targeted therapies for various tumors.However,the similarities and discrepancies in characteristics of RHOA mutations among various histologic subtypes of PTCL have not been fully elucidated.Herein we highlight the inconsistencies and complexities of the type and location of RHOA mutations and demonstrate the contribution of RHOA variants to the pathogenesis of PTCL by combining epigenetic abnormalities and activating multiple downstream pathways.The promising potential of targeting RHOA as a therapeutic modality is also outlined.This review provides new insight in the field of personalized medicine to improve the clinical outcomes for patients.

Survey on hospice care attitude of family members of advanced cancer patients at different ages

BACKGROUND Hospice care plays an important role in improving the quality of life of advanced cancer patients,but controversy remains over whether age affects the attitudes of family members toward hospice care.AIM To investigate the attitudes of family members of advanced cancer patients of different ages toward hospice care.METHODS The study participants were 175 family members of patients with advanced cancer from January 2020 and October 2022.The participants were divided into youth(<40 years,n=65),middle-aged(40–60 years,n=59),and elderly(>60 years,n=51)groups.Researchers investigated and compared the degree of awareness regarding hospice care,attitudes,and whether the family members of patients would choose hospice care.RESULTS Among the family members of 175 patients,approximately 28%(49/175)were aware of hospice care.Awareness of hospice care,the proportion of hospice care acceptance and adaptation attitudes,and the proportion of those who chose hospice care in the youth group were higher in the middle-aged and elderly groups(P<0.05).No statistically significant difference was found in these three indicators between the middle-aged and elderly groups(P>0.05).Hospice care was chosen mainly to relieve pain and reduce unnecessary treatment,whereas the reasons for not choosing hospice care were mainly distrust and ethical concerns.CONCLUSION The family members of patients with advanced cancer had relatively low awareness of hospice care,while youth had a higher awareness of hospice care,acceptance,and adaptation attitudes,and were more willing to choose hospice care.

Depositional model of the Member Deng-2 marginal microbial mound-bank complex of the Dengying Formation in the southwestern Sichuan Basin, SW China: Implications for the Ediacaran microbial mound construction and hydrocarbon exploration

Recent advances in hydrocarbon exploration have been made in the Member Deng-2 marginal microbial mound-bank complex reservoirs of the Dengying Formation in the western Sichuan Basin, SW China,where the depositional process is regarded confusing. The microfacies, construction types, and depositional model of the Member Deng-2 marginal microbial mound-bank complex have been investigated using unmanned aerial vehicle photography, outcrop section investigation, thin section identification,and seismic reflections in the southwestern Sichuan Basin. The microbialite lithologic textures in this region include thrombolite, dendrolite, stromatolite, fenestral stromatolite, spongiostromata stone,oncolite, aggregated grainstone, and botryoidal grapestone. Based on the comprehensive analysis of“depositional fabrics-lithology-microfacies”, an association between a fore mound, mound framework,and back mound subfacies has been proposed based on water depth, current direction, energy level and lithologic assemblages. The microfacies of the mound base, mound core, mound flank, mound cap, and mound flat could be recognized among the mound framework subfacies. Two construction types of marginal microbial mound-bank complex have been determined based on deposition location, mound scale, migration direction, and sedimentary facies association. Type Jinkouhe microbial mound constructions(TJMMCs) develop along the windward margin owing to their proximity to the seaward subfacies fore mound, with a northeastwardly migrated microbial mound on top of the mud mound,exhibiting the characteristics of large-sized mounds and small-sized banks in the surrounding area. Type E'bian microbial mound constructions(TEMMCs) primarily occur on the leeward margin, resulting from the presence of onshore back mound subfacies, with the smaller southwestward migrated microbial mounds existing on a thicker microbial flat. The platform margin microbial mound depositional model can be correlated with certain lateral comparison profile and seismic reflection structures in the 2D seismic section, which can provide references for future worldwide exploration. Microbial mounds with larger buildups and thicker vertical reservoirs are typically targeted on the windward margin, while small-sized microbial mounds and flats with better lateral connections are typically focused on the leeward margin.

Types,composition and diagenetic evolution of authigenic clay minerals in argillaceous limestone of sepiolite-bearing strata:A case study of Mao-1 Member of Middle Permian Maokou Formation,eastern Sichuan Basin,SW China

The types,occurrence and composition of authigenic clay minerals in argillaceous limestone of sepiolite-bearing strata of the first member of the Middle Permian Maokou Formation(Mao-1 Member)in eastern Sichuan Basin were investigated through outcrop section measurement,core observation,thin section identification,argon ion polishing,X-ray diffraction,scanning electron microscope,energy spectrum analysis and laser ablation-inductively coupled plasma-mass spectrometry.The diagenetic evolution sequence of clay minerals was clarified,and the sedimentary-diagenetic evolution model of clay minerals was established.The results show that authigenic sepiolite minerals were precipitated in the Si4+and Mg2+-rich cool aragonite sea and sepiolite-bearing strata were formed in the Mao-1 Member.During burial diagenesis,authigenic clay minerals undergo two possible evolution sequences.First,from the early diagenetic stage A to the middle diagenetic stage A1,the sepiolite kept stable in the shallow-buried environment lack of Al3+.It began to transform into stevensite in the middle diagenetic stage A2,and then evolved into disordered talc in the middle diagenetic stage B1and finally into talc in the period from the middle diagenetic stage B2to the late diagenetic stage.Thus,the primary diagenetic evolution sequence of authigenic clay minerals,i.e.sepiolite-stevensite-disordered talc-talc,was formed in the Mao-1 Member.Second,in the early diagenetic stage A,as Al3+carried by the storm and upwelling currents was involved in the diagenetic process,trace of sepiolite started to evolve into smectite,and a part of smectite turned into chlorite.From the early diagenetic stage B to the middle diagenesis stage A1,a part of smectite evolved to illite/smectite mixed layer(I/S).The I/S evolved initially into illite from the middle diagenesis stage A2to the middle diagenesis stage B2,and then totally into illite in the late diagenesis stage.Thus,the secondary diagenetic evolution sequence of authigenic clay minerals,i.e.sepiolite-smectite-chlorite/illite,was formed in the Mao-1 Member.The types and evolution of authigenic clay minerals in argillaceous limestone of sepiolite-bearing strata are significant for petroleum geology in two aspects.First,sepiolite can adsorb and accumulate a large amount of organic matters,thereby effectively improving the quality and hydrocarbon generation potential of the source rocks of the Mao-1 Member.Second,the evolution from sepiolite to talc is accompanied by the formation of numerous organic matter pores and clay shrinkage pores/fractures,as well as the releasing of the Mg2+-rich diagenetic fluid,which allows for the dolomitization of limestone within or around the sag.As a result,the new assemblages of self-generation and self-accumulation,and lower/side source and upper/lateral reservoir,are created in the Middle Permian,enhancing the hydrocarbon accumulation efficiency.

Potential role of transmembrane 9 superfamily member 1 as a biomarker in urothelial cancer

Bladder cancer is a urological tumor with high rates of recurrence despite recent advances in novel therapies.Many proteins involved in the molecular mechanisms are currently an enigma,especially the transmembrane 9 superfamily member 1 which has an unclear function.Wei et al published the function and mechanism of this protein,and showed that it could participate in the proliferation,migration and invasion of tumor cells in bladder cancer,therefore treatments directed against this protein may be beneficial in avoiding this condition.

Understanding the role of transmembrane 9 superfamily member 1 in bladder cancer pathogenesis

In this editorial we comment on the article by Wei et al,published in the recent issue of the World Journal of Clinical Oncology.The authors investigated the role of Transmembrane 9 superfamily member 1(TM9SF1)protein in bladder cancer(BC)carcinogenesis.Lentiviral vectors were used to achieve silencing or overexpression of TM9SF1 gene in three BC cell lines.These cell lines were then subject to cell counting kit 8,wound-healing assay,transwell assay,and flow cytometry.Proliferation,migration,and invasion of BC cells were increased in cell lines subjected to TM9SF1 overexpression.TM9SF1 silencing inhibited proliferation,migration and invasion of BC cells.The authors conclude that TM9SF1 may be an oncogene in bladder cancer pathogenesis.

Analysis of the Current Status and Influencing Factors of Family Members’Collateral Stigma in Schizophrenia Patients

Objective: To investigate the associated collateral stigma of the family members of schizophrenia patients and analyze its current status and influencing factors. Methods: The Link Depreciation-Discrimination Perception Scale was used to investigate the status quo of the associated stigma of the family members of 169 schizophrenia patients diagnosed in 4 hospitals in a certain province. The results of the investigation were analyzed and summarized. Results: The detection rate of stigma associated with the family members of schizophrenia patients was 72.78%, with a score of 28.41 ± 3.92 points. The main influencing factors were the family member’s education level, the patient’s illness duration, the family member’s occupation, and the family-patient relationship. Conclusion: The detection rate of stigma associated with schizophrenia was relatively high. This requires increased attention and appropriate nursing intervention.

简牍所见秦及西汉初期的未成年“户人”研究

秦及西汉初期的简牍中出现了一些未成年“户人”,他们均为男性。未成年人成为“户人”的主要影响因素包括性别、尊卑及家庭规模等,年龄不是重要考量因素。未成年男性或担任“户人”,或提前服役,过于繁重的赋役迫使未成年男性作为劳动力的价值得到提升。未成年人“户人”被提前成人化,既体现了国家在行政管理上对个人身份信息的漠视,也说明了皇帝和官僚集团极尽可能压榨人民的剩余劳动。

四川盆地东部侏罗系陆相页岩优质储层发育机理及其油气地质意义

四川盆地东部复兴地区涪页10井在侏罗系自流井组东岳庙段测试获得高产油气流,进一步证实了陆相页岩具有巨大的油气勘探潜力。但东岳庙段页岩具有高黏土矿物含量、富介壳夹层和较高有机质热演化程度等特殊性,是一种新类型陆相页岩,其优质储层发育特征及影响因素尚缺乏系统研究。为此,综合利用X射线衍射、薄片鉴定、氩离子抛光扫描电镜、压汞—液氮吸附联测、主微量元素分析和纳米CT等多技术手段,深入剖析了东岳庙段陆相页岩储层发育特征,探讨了优质页岩储层发育的控制因素,最后明确了其油气地质意义。研究结果表明:(1)东岳庙段陆相页岩具有“多孔缝共生—无机孔为主—大孔大喉”的储集空间发育特征,发育以黏土矿物晶间孔为主的8种类型孔隙和成岩收缩缝、生烃超压缝等类型微裂缝,无机孔占比为80%~90%,孔隙结构以介孔和宏孔为主,页岩孔喉半径较大,纹层状介壳/粉砂黏土页岩相和纹层状介壳黏土页岩相2种纹层状页岩具有较好的烃源岩品质,为东岳庙段优质储层类型;(2)纹层状介壳黏土页岩相储层具有“高黏土矿物扩孔—高演化生孔—层偶界面成缝—超压保孔缝”的成储机制,沉积环境控制了纵向上页岩垂向非均质性,高黏土矿物含量对增大储集空间有积极作用,半深湖沉积环境保障了优质岩相的发育,纹层沉积构造与页岩形成的层偶界面为应力弱面,易形成微裂缝;(3)较高有机质热演化阶段发生的黏土矿物转化作用、有机质热解和溶蚀作用直接影响了“大孔大喉”的储集空间特征,超压系统、致密介壳隔层为孔缝的保持奠定了保存基础。结论认为,取得的研究成果进一步完善了陆相复杂岩相页岩优质储层发育机理认识,丰富了中国陆相页岩油气地质理论,为新类型陆相页岩油气的勘探选区、选层提供了理论指导。

苏北盆地低有机质断块型页岩油测井评价方法及应用

苏北盆地构造复杂、断层发育,阜宁组二段泥页岩的有机质丰度较低,总有机碳含量基本小于1.5%。该套泥页岩具有岩相类型多样、孔隙结构复杂、储层非均质性强、压力系数横向变化大等特点。以苏北盆地H区块阜宁组二段页岩油储层为例,基于岩石物理实验分析结果,对区域测井响应关系特征进行分析,将常规测井与特殊测井相结合,建立页岩油储层岩性、储集性、含油性、可动性、可压性评价的测井解释模型,模型计算结果与岩心分析结果吻合较好。在此基础上,优选敏感参数,建立了区块页岩油储层评价分类标准,对储层进行“甜点”综合评价。经多口井的勘探实践验证,该测井评价技术具有较好的地区适应性,有效划分页岩油储层类别,有利于页岩油储层“甜点”优选,为苏北盆地页岩油勘探开发提供可靠的技术支持。

松辽盆地南部姚家组下段层间氧化特征及其与铀成矿关系研究

基于松辽盆地南部历年砂岩型铀矿调查评价与勘查资料,文章研究总结了上白垩统姚家组下段层间氧化蚀变分带特征及其成因,深入分析了氧化带类型及其空间展布规律,进而研究了不同类型氧化带与铀矿化关系。研究表明,松辽盆地南部层间氧化作用存在强、弱之分,形成时间对应有早、晚之别,其氧化带空间产出复杂多样,总体形成了具有“上、下氧化,中间还原”的地球化学环境,一般靠近完全氧化带边界附近的还原性灰色砂体内形成板状铀矿体,铀矿化规模相对较大,分布范围广,找矿空间巨大。通过对比不同地区姚家组下段氧化带与铀矿化空间匹配关系,初步认为松辽盆地南部局部地区还存在传统意义上舌状层间氧化作用,形成了氧化带前锋线卷状铀矿体,其控制形成的铀矿化宽度虽相对较窄,但新成矿类型的补充将极大拓宽盆地找矿空间,对外围找矿再突破奠定了新的理论基础。

四川盆地东部复兴地区侏罗系东岳庙段页岩热演化史及油气地质意义

页岩热演化史与油气生成过程、微观孔隙的形成演化以及油气的富集成藏密切相关,是准确评价页岩油气资源潜力、揭示成藏富集规律的关键参数。为了准确评价四川盆地东部复兴地区侏罗系东岳庙段页岩热演化史、含油气特征及其与海相页岩气的差异,结合研究区典型井地质、测井及地球化学资料,利用BasinMod软件模拟了东岳庙段页岩的埋藏史、热演化史和生烃史,探讨了页岩热演化与油气生成、孔隙演化、成藏富集之间的关系。研究结果表明:(1)研究区东岳庙段页岩在侏罗纪中期进入生烃门限,侏罗纪中晚期达到主生油高峰,并存在两个生烃高峰,分别为侏罗纪末期和白垩纪中期;(2)第一个生烃高峰期以生油为主,持续时间长且生烃速率较快,第二个生烃高峰期以生成凝析油和湿气为主,持续时间较短且生烃强度较低;(3)东岳庙段页岩的平均累计生油强度为9.5×10^(8)t/km^(2),平均累计生气强度为2.5×10^(8)m^(3)/km^(2);(4)东岳庙段页岩孔隙演化过程可分为4个阶段,分别为早期压实减孔阶段、液态烃滞留堵孔阶段、热裂解有机孔形成阶段和热裂解生湿气阶段;(5)压实作用和液态烃滞留堵孔是导致页岩原生孔隙快速减少的主要原因,有机质(主要为固体沥青)与黏土混杂堆积形成的有机黏土复合体是东岳庙段页岩重要的储集空间。结论认为:(1)不同构造部位的埋藏史、热演化史和生烃史影响了页岩油气的生成、排放与滞留,决定了现今页岩油气的含量差异,抬升剥蚀厚度较小且保存条件较好的地区,具有较高的油气滞留量,值得高度关注;(2)成果认识坚定了川东地区陆相页岩油气勘探开发信心,为其他类似地质情况的探区提供了有益借鉴。

回应性制度赋能:新时代基层党员领导干部政治能力建设的制度分析

党的事业,根基在基层,关键在干部。随着中国特色社会主义进入新时代,发展阶段的升级、主要矛盾的转化、奋斗目标的转向以及发展环境的不确定性,给基层政治接续有效带来了新挑战,基层党员领导干部政治能力建设成为新时代中国基层政治接续有效的核心议题。从“政党回应、制度赋能与有效政治”的分析框架透视,新时代基层党员领导干部政治能力建设,实际上是党回应时代环境、时代使命和时代需求的变化,坚持和完善党的政治能力建设制度,创新制度赋能的组织实践和自我实践,提高基层党员领导干部政治能力,实现基层有效政治的政治行动。新时代新征程应以基层政治的有效性为导向,持续加强基层党员领导干部政治能力提升的制度建设,积极构建“内外双生、多层复合”的均衡性制度框架。

输电塔半刚性连接钢管构件轴压稳定性能研究

为了研究输电塔半刚性连接钢管构件的轴压稳定性能,对60根钢管试件进行了轴压试验,分析了其失稳破坏模式和稳定承载力,并与现行设计规范计算的承载力进行了对比;建立了半刚性连接钢管构件的有限元模型,经与试验结果对比验证后开展了参数分析,研究了初始转动刚度、径厚比、材料强度以及初始缺陷对半刚性连接钢管构件的稳定系数-长细比曲线的影响;基于有限元分析的结果提出了半刚性连接钢管构件的轴压稳定计算公式。研究结果表明:半刚性连接构件的试验稳定承载力均大于铰接构件的试验稳定承载力,在所有试验工况中两者的相对差距最小为5.6%;中国和美国现行设计规范计算得到的钢管构件稳定承载力总体偏保守,分别约为试验结果的0.78倍和0.89倍;影响半刚性连接钢管构件稳定承载力的主要因素为长细比、初始转动刚度以及边界条件;提出的半刚性连接钢管构件轴压稳定计算公式能准确地预测其稳定系数并具有良好的适用性,可为实际工程提供参考。

陆相淡水湖盆细粒沉积成因机制及页岩油储集特征——以鄂尔多斯盆地西南部三叠系延长组长7_3亚段为例

基于鄂尔多斯盆地西南部三叠系延长组长73亚段页岩油勘探实践进展,综合野外露头、钻井、测井、岩心、地球化学等资料,以及水槽实验模拟等技术手段,开展陆相湖盆细粒沉积古环境、岩相组合和分布、沉积成因及页岩油储集特征的系统研究。结果表明:①长73亚段沉积期研究区古环境整体表现为温暖湿润、梅雨事件频发、水体深度较大的淡水湖盆特征,古地貌呈现西南陡、东北缓的不对称型,可进一步细分为湖底深洼、古沟道、湖底古脊等微古地貌单元。②长73亚段发育一套极细砂岩、粉砂岩、泥页岩、凝灰岩等细粒沉积,垂向多呈薄互层—纹层组合分布,砂岩粒径大多小于62.5μm,单层厚度为0.05~0.64 m,含完整植物碎片,发育波状层理、逆粒序-正粒序组合、爬升沙纹层理等沉积构造,揭示异重流沉积成因。③环形水槽模拟实验再现了异重流搬运过程及沉积特征,主要表现为初期的密度流速差造成头部较厚且粒径较大,上部较薄且粒径偏小的特征;中期滑水作用促使流体头部抬升并向前快速搬运,由此产生的多个“新前端”促使粉砂岩、泥质粉砂岩等细粒砂岩向湖盆中部长距离搬运。④明确了盆地西南部细粒砂质岩以异重流成因为主,指出频繁发育的洪水事件、西南部陡坡地形是异重流发育的主控因素。⑤长73亚段砂岩、泥页岩发育微纳米孔喉系统,不同岩性均含油,但可动油含量差异大,砂岩中可动油含量最大。⑥明确了长73亚段多期异重流砂岩与泥页岩形成的细粒沉积复合体具有“整体含油、差异储集”特征,低TOC泥岩与粉砂岩组合为目前最有利的勘探目标。