Menaquinone-7原料药在避光条件下经无水乙醇超声溶解后,采用Agilent Eclipse plus C_(18)色谱柱(2.1mm×50mm,1.8μm)分离主成分及有关物质,以甲醇-水(98+2)混合液为流动相,分别在波长246,270nm处测定主成分及有关物质。Menaquinon...Menaquinone-7原料药在避光条件下经无水乙醇超声溶解后,采用Agilent Eclipse plus C_(18)色谱柱(2.1mm×50mm,1.8μm)分离主成分及有关物质,以甲醇-水(98+2)混合液为流动相,分别在波长246,270nm处测定主成分及有关物质。Menaquinone-7的线性范围在0.005~0.400g·L^(-1)内,检出限(3S/N)为0.1mg·L^(-1)。加标回收率在98.8%~100%之间,测定值的相对标准偏差(n=6)小于1.0%。测得两批Menaquinone-7原料药中主成分的质量分数分别为98.0%,98.2%,单个杂质质量分数小于0.5%,杂质总质量分数小于1.0%。展开更多
Given that vascular calcification is inversely correlated with the clinical intake of menaquinone, a rat model of warfarin-induced calcification may be useful for testing menaquinone and vitamin K-1 potential effects ...Given that vascular calcification is inversely correlated with the clinical intake of menaquinone, a rat model of warfarin-induced calcification may be useful for testing menaquinone and vitamin K-1 potential effects on vascular function. The aim of the present study was to investigate effects of vitamin K-1 and menaquinone-7 treatments on blood pressure and vascular function in warfarin-induced vascular calcification model during five-week intervention in normotensive Wistar-Kyoto rats. Blood pressure was measured weekly, and at the end of the intervention in vitro vascular reactivity measurements were done. Alizarin Red S and von Kossa stainings were used to record possible calcification of aortic sections. Routine clinical chemistry was done from serum and urine samples. Vascular calcification was seen only in a few warfarin-treated animals in histological staining. Warfarin-treatment did not change significantly blood pressure of the rats. Warfarin-treatment increased slightly the endothelium-dependent relaxation of aorta after the L-type calcium channels were blocked. Also the vascular relaxation improved after NOS inhibition in the aorta of the healthy controls and menaquinone-7 treated animals, indicating that the relaxation in those groups was not totally dependent on NO. Clinical chemistry from serum showed some differences in urea, creatinine as well as lipid and glucose metabolism between the healthy controls and warfarin-treated rats.展开更多
Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report...Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial cells.Both the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 phenotype.In addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and YM1.Taken together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.展开更多
A menaquinone-7（MK-7） high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK...A menaquinone-7（MK-7） high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma（ARTP） mutation breeding system. We isolated an MK-7-producing strain Y-2 and identified it as Bacillus amyloliquefaciens, which produced（7.1±0.5） mg/L of MK-7 with maize meal hydrolysate as carbon source. Then, an MK-7 highproducing strain B. amyloliquefaciens H.β.D.R.-5 with resistance to 1-hydroxy-2-naphthoic acid, β-2-thienylalanine, and diphenylamine was obtained from the mutation of the strain Y-2 using an ARTP mutation breeding system. Using strain H.β.D.R.-5, efficient production of MK-7 was achieved（（30.2±2.7） mg/L）. In addition, the effects of nitrogen sources, prenyl alcohols, and MgSO_4 on MK-7 production were investigated, suggesting that soymeal extract combined with yeast extract, isopentenol, and MgSO4 was beneficial. Under the optimized condition, the MK-7 production and biomass-specific yield reached（61.3±5.2） mg/L and 2.59 mg/L per OD600 unit respectively in a 7-L fermenter. These results demonstrated that strain H.β.D.R.-5 has the capacity to produce MK-7 from maize meal hydrolysate, which could reduce the substrate cost.展开更多
棉田虫害的快速检测与准确识别是预防棉田虫害、提高棉花品质的重要前提。针对真实棉田环境下昆虫相似度高、背景干扰严重的问题,该研究提出一种ECSF-YOLOv7棉田虫害检测模型。首先,采用EfficientFormerV2作为特征提取网络,以加强网络...棉田虫害的快速检测与准确识别是预防棉田虫害、提高棉花品质的重要前提。针对真实棉田环境下昆虫相似度高、背景干扰严重的问题,该研究提出一种ECSF-YOLOv7棉田虫害检测模型。首先,采用EfficientFormerV2作为特征提取网络,以加强网络的特征提取能力并减少模型参数量;同时,将卷积注意力模块(convolution block attention module,CBAM)嵌入到模型的主干输出端,以增强模型对小目标的特征提取能力并削弱背景干扰;其次,使用GSConv卷积搭建Slim-Neck颈部网络结构,在减少模型参数量的同时保持模型的识别精度;最后,采用Focal-EIOU(focal and efficient IOU loss,Focal-EIOU)作为边界框回归损失函数,加速网络收敛并提高模型的检测准确率。结果表明,改进的ECSF-YOLOv7模型在棉田虫害测试集上的平均精度均值(mean average precision,mAP)为95.71%,检测速度为69.47帧/s。与主流的目标检测模型YOLOv7、SSD、YOLOv5l和YOLOX-m相比,ECSF-YOLOv7模型的mAP分别高出1.43、9.08、1.94、1.52个百分点,并且改进模型具有参数量更小、检测速度更快的优势,可为棉田虫害快速准确检测提供技术支持。展开更多
文摘Menaquinone-7原料药在避光条件下经无水乙醇超声溶解后,采用Agilent Eclipse plus C_(18)色谱柱(2.1mm×50mm,1.8μm)分离主成分及有关物质,以甲醇-水(98+2)混合液为流动相,分别在波长246,270nm处测定主成分及有关物质。Menaquinone-7的线性范围在0.005~0.400g·L^(-1)内,检出限(3S/N)为0.1mg·L^(-1)。加标回收率在98.8%~100%之间,测定值的相对标准偏差(n=6)小于1.0%。测得两批Menaquinone-7原料药中主成分的质量分数分别为98.0%,98.2%,单个杂质质量分数小于0.5%,杂质总质量分数小于1.0%。
基金DuPont Nutrition Health, Finska Läkaresalskapet Finnish Clinical Chemistry Foundation
文摘Given that vascular calcification is inversely correlated with the clinical intake of menaquinone, a rat model of warfarin-induced calcification may be useful for testing menaquinone and vitamin K-1 potential effects on vascular function. The aim of the present study was to investigate effects of vitamin K-1 and menaquinone-7 treatments on blood pressure and vascular function in warfarin-induced vascular calcification model during five-week intervention in normotensive Wistar-Kyoto rats. Blood pressure was measured weekly, and at the end of the intervention in vitro vascular reactivity measurements were done. Alizarin Red S and von Kossa stainings were used to record possible calcification of aortic sections. Routine clinical chemistry was done from serum and urine samples. Vascular calcification was seen only in a few warfarin-treated animals in histological staining. Warfarin-treatment did not change significantly blood pressure of the rats. Warfarin-treatment increased slightly the endothelium-dependent relaxation of aorta after the L-type calcium channels were blocked. Also the vascular relaxation improved after NOS inhibition in the aorta of the healthy controls and menaquinone-7 treated animals, indicating that the relaxation in those groups was not totally dependent on NO. Clinical chemistry from serum showed some differences in urea, creatinine as well as lipid and glucose metabolism between the healthy controls and warfarin-treated rats.
基金supported by the National Natural Science Foundation of China,Nos.82171429,81771384a grant from Wuxi Municipal Health Commission,No.1286010241190480(all to YS)。
文摘Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial cells.Both the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 phenotype.In addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and YM1.Taken together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.
基金supported by the Natural Science Foundation of Jiangsu Province(No.BK20150149),China
文摘A menaquinone-7（MK-7） high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma（ARTP） mutation breeding system. We isolated an MK-7-producing strain Y-2 and identified it as Bacillus amyloliquefaciens, which produced（7.1±0.5） mg/L of MK-7 with maize meal hydrolysate as carbon source. Then, an MK-7 highproducing strain B. amyloliquefaciens H.β.D.R.-5 with resistance to 1-hydroxy-2-naphthoic acid, β-2-thienylalanine, and diphenylamine was obtained from the mutation of the strain Y-2 using an ARTP mutation breeding system. Using strain H.β.D.R.-5, efficient production of MK-7 was achieved（（30.2±2.7） mg/L）. In addition, the effects of nitrogen sources, prenyl alcohols, and MgSO_4 on MK-7 production were investigated, suggesting that soymeal extract combined with yeast extract, isopentenol, and MgSO4 was beneficial. Under the optimized condition, the MK-7 production and biomass-specific yield reached（61.3±5.2） mg/L and 2.59 mg/L per OD600 unit respectively in a 7-L fermenter. These results demonstrated that strain H.β.D.R.-5 has the capacity to produce MK-7 from maize meal hydrolysate, which could reduce the substrate cost.
文摘棉田虫害的快速检测与准确识别是预防棉田虫害、提高棉花品质的重要前提。针对真实棉田环境下昆虫相似度高、背景干扰严重的问题,该研究提出一种ECSF-YOLOv7棉田虫害检测模型。首先,采用EfficientFormerV2作为特征提取网络,以加强网络的特征提取能力并减少模型参数量;同时,将卷积注意力模块(convolution block attention module,CBAM)嵌入到模型的主干输出端,以增强模型对小目标的特征提取能力并削弱背景干扰;其次,使用GSConv卷积搭建Slim-Neck颈部网络结构,在减少模型参数量的同时保持模型的识别精度;最后,采用Focal-EIOU(focal and efficient IOU loss,Focal-EIOU)作为边界框回归损失函数,加速网络收敛并提高模型的检测准确率。结果表明,改进的ECSF-YOLOv7模型在棉田虫害测试集上的平均精度均值(mean average precision,mAP)为95.71%,检测速度为69.47帧/s。与主流的目标检测模型YOLOv7、SSD、YOLOv5l和YOLOX-m相比,ECSF-YOLOv7模型的mAP分别高出1.43、9.08、1.94、1.52个百分点,并且改进模型具有参数量更小、检测速度更快的优势,可为棉田虫害快速准确检测提供技术支持。
