The chemical composition of essential oils obtained from Artemisia herba-alba and Mentha pulegium were determined. The essential oils were analyzed by gas chromatography coupled with mass spectrometry （GC/MS）. Their...The chemical composition of essential oils obtained from Artemisia herba-alba and Mentha pulegium were determined. The essential oils were analyzed by gas chromatography coupled with mass spectrometry （GC/MS）. Their antibacterial activity was studied in vitro against three standard strains： E. coli ATCC 25922, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, and five clinical strains： Enterobacter cloacae, Staphylococcus aureus, Pseudomonas pyocyanique, Enterococcus faecium, and E. coli. Nineteen constituents were identified in A. herba-alba essential oil representing 99.57% of the total composition The major component was α-thujone （59.07%）. The bacterial strains were inhibited at concentrations ranging from 1.25 μL/mL to 5μL/mL and killed at concentrations ranging from 1.25 μL/mL to 10 μL/mL. M. pulegium resulted in the identification of eighteen constituents representing 99.48% of the total composition. The main component was pulegone （78.07%）. The minimal inhibitory （MIC） and bactericidal （MBC） concentrations were ranging from 1.25 μL/mL to 2.5 μL/mL.展开更多
The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. Th...The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.展开更多
Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides(T. satureioides) and Mentha pulegium(...Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides(T. satureioides) and Mentha pulegium(M. pulegium), and to study their chemical composition.Methods: The aerial parts of T. satureioides and M. pulegium(leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution method.Results: Chemical analysis of their aerial part's essential oils gave as major compounds,borneol(34.26%), carvacrol(31.21%) and thymol(3.71%) for T. satureioides and R(+)-pulegone(75.48%), carvone(6.66%) and dihydrocarvone(4.64%) for M. pulegium.Thereafter their antimycobacterial effect evaluation, using the micro-dilution method,indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015%(v/v) and from 0.125% to 0.031%(v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium smegmatis.Conclusions: It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug.展开更多
文摘The chemical composition of essential oils obtained from Artemisia herba-alba and Mentha pulegium were determined. The essential oils were analyzed by gas chromatography coupled with mass spectrometry （GC/MS）. Their antibacterial activity was studied in vitro against three standard strains： E. coli ATCC 25922, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, and five clinical strains： Enterobacter cloacae, Staphylococcus aureus, Pseudomonas pyocyanique, Enterococcus faecium, and E. coli. Nineteen constituents were identified in A. herba-alba essential oil representing 99.57% of the total composition The major component was α-thujone （59.07%）. The bacterial strains were inhibited at concentrations ranging from 1.25 μL/mL to 5μL/mL and killed at concentrations ranging from 1.25 μL/mL to 10 μL/mL. M. pulegium resulted in the identification of eighteen constituents representing 99.48% of the total composition. The main component was pulegone （78.07%）. The minimal inhibitory （MIC） and bactericidal （MBC） concentrations were ranging from 1.25 μL/mL to 2.5 μL/mL.
基金the support of the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(CNPq) the Fundacao de Amparo a Pesquisa do Estado de Minas Gerais(FAPEMIG)for financial support the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES)for a PVNS.
文摘The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.
基金Supported by the Cooperation for Innovation in the Agro-Food Domain Project within the 7th Research and Development Framework Programme FP7-INCO-2013-9(Grant agreement number 609495)
文摘Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides(T. satureioides) and Mentha pulegium(M. pulegium), and to study their chemical composition.Methods: The aerial parts of T. satureioides and M. pulegium(leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution method.Results: Chemical analysis of their aerial part's essential oils gave as major compounds,borneol(34.26%), carvacrol(31.21%) and thymol(3.71%) for T. satureioides and R(+)-pulegone(75.48%), carvone(6.66%) and dihydrocarvone(4.64%) for M. pulegium.Thereafter their antimycobacterial effect evaluation, using the micro-dilution method,indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015%(v/v) and from 0.125% to 0.031%(v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium smegmatis.Conclusions: It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug.
