美国冰、云和陆地高程二号卫星(The Ice,Cloud,and Land Elevation Satellite-2,ICESat-2)是ICESat卫星的继任者,旨在监测地球的冰盖、冰川、海洋和陆地高程的变化等,其携带的地形激光高度计系统(ATLAS)发射532 nm波长的激光,具备一定...美国冰、云和陆地高程二号卫星(The Ice,Cloud,and Land Elevation Satellite-2,ICESat-2)是ICESat卫星的继任者,旨在监测地球的冰盖、冰川、海洋和陆地高程的变化等,其携带的地形激光高度计系统(ATLAS)发射532 nm波长的激光,具备一定的水体穿透能力。作为光子计数式激光雷达,ICESat-2的数据易受外界环境影响而接收到大量噪声光子,导致光子数据密度分布不均匀。本文提出了一种基于密度峰值聚类(Density Peak Clustering,DPC)算法的光子去噪方法,通过数据集的欧式距离计算局部密度作为点云数据的属性,采用基尼指数自适应选择最优截断距离,分别对日间和夜间数据进行多次实验,得出了两类数据的局部密度阈值参数。本文选取三处实验区域进行信号光子去噪分析,使用本文方法的去噪精度F值优于官方置信度标签去噪和传统密度聚类算法(Density-Based Spatial Clustering of Applications with Noise,DBSCAN),可以应用于星载激光雷达数据去噪处理。最后,对去噪后的华光礁区域信号光子进行折射校正,与收集的DEM数据进行对比可见,结合本文去噪方法可以使用ICESat-2数据进行浅水域的水深测量。展开更多
The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt ...The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt cotton breeding and high-yield and-efficiency cotton cultivation.This study was conducted using Bt cotton cultivar‘Sikang 3'during the 2020 and 2021 growing seasons at Yangzhou University Farm,Yangzhou,Jiangsu Province,China.Potted cotton plants were exposed to high temperature and drought stress,and sprayed with either 20 mg L^(-1)DPC or water(CK).Seven days after treatment,the Cry1Ac protein content,α-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content were measured,and transcriptome sequencing was performed.DESeq was used for differential gene analysis.Under the DPC treatment,the Cry1Ac protein content increased by 4.7-11.9% compared to CK.Theα-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content all increased.Transcriptome analysis revealed 7,542 upregulated genes and 10,449 downregulated genes for DPC vs.CK.Gene ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analyses showed that the differentially expressed genes were mainly involved in biological processes,such as carbon and amino acid metabolism.For example,genes encoding 6-phosphofructokinase,pyruvate kinase,glutamic pyruvate transaminase,pyruvate dehydrogenase,citrate synthase,isocitrate dehydrogenase,2-oxoglutarate dehydrogenase,glutamate synthase,1-pyrroline-5-carboxylate dehydrogenase,glutamic oxaloacetic transaminase,amino-acid N-acetyltransferase,and acetylornithine deacetylase were all significantly upregulated.The DPC treatment increased pyruvate,α-ketoglutarate,and oxaloacetate by increasing the operational rate of the glycolytic pathway of the citric acid cycle.It also significantly upregulated the genes encoding glutamate synthase,pyrrolidine-5-carboxylic acid dehydrogenase,glutamate oxaloacetate transaminase,and N-acetylglutamate synthetase,while it downregulated the genes encoding glutamine synthetase.Therefore,the synthesis of aspartic acid,glutamic acid,pyruvate,and arginine increased after treatment with DPC,and the Cry1Ac protein content was increased by regulating carbon and amino acid metabolism.展开更多
火电机组磨煤机存在运行条件恶劣、故障频发等问题,对磨煤机进行故障预警,可以有效防止一些常见故障的发生,从而保证火电机组的安全运行。为此,提出一种基于相互邻近度的密度峰值聚类和多元状态估计的磨煤机故障预警方法。首先,采用核...火电机组磨煤机存在运行条件恶劣、故障频发等问题,对磨煤机进行故障预警,可以有效防止一些常见故障的发生,从而保证火电机组的安全运行。为此,提出一种基于相互邻近度的密度峰值聚类和多元状态估计的磨煤机故障预警方法。首先,采用核主元分析选取磨煤机的主要状态参数,同时采用集合经验模态分解对历史运行数据进行去噪,进一步优化数据质量;然后,采用基于相互邻近度的密度峰值聚类(density peaks clustering based on mutual neighborhood degrees,DPC-MND)方法构建动态记忆矩阵,利用多元状态估计技术(multivariate state estimation techniques,MSET)对磨煤机正常运行工况下的历史数据进行建模,并确定磨煤机的运行状态。最后,以安徽某电厂ZGM113G型中速磨煤机为例进行验证,结果表明该方法可以实现对磨煤机故障的有效预警。展开更多
文摘美国冰、云和陆地高程二号卫星(The Ice,Cloud,and Land Elevation Satellite-2,ICESat-2)是ICESat卫星的继任者,旨在监测地球的冰盖、冰川、海洋和陆地高程的变化等,其携带的地形激光高度计系统(ATLAS)发射532 nm波长的激光,具备一定的水体穿透能力。作为光子计数式激光雷达,ICESat-2的数据易受外界环境影响而接收到大量噪声光子,导致光子数据密度分布不均匀。本文提出了一种基于密度峰值聚类(Density Peak Clustering,DPC)算法的光子去噪方法,通过数据集的欧式距离计算局部密度作为点云数据的属性,采用基尼指数自适应选择最优截断距离,分别对日间和夜间数据进行多次实验,得出了两类数据的局部密度阈值参数。本文选取三处实验区域进行信号光子去噪分析,使用本文方法的去噪精度F值优于官方置信度标签去噪和传统密度聚类算法(Density-Based Spatial Clustering of Applications with Noise,DBSCAN),可以应用于星载激光雷达数据去噪处理。最后,对去噪后的华光礁区域信号光子进行折射校正,与收集的DEM数据进行对比可见,结合本文去噪方法可以使用ICESat-2数据进行浅水域的水深测量。
基金supported by the National Natural Science Foundation of China(31901462)the Natural Science Foundation of the Jiangsu Higher Education Institutions,China(22KJA210005)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD)the Brand Professional Construction Program of Jiangsu Higher Education Institutions,China。
文摘The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt cotton breeding and high-yield and-efficiency cotton cultivation.This study was conducted using Bt cotton cultivar‘Sikang 3'during the 2020 and 2021 growing seasons at Yangzhou University Farm,Yangzhou,Jiangsu Province,China.Potted cotton plants were exposed to high temperature and drought stress,and sprayed with either 20 mg L^(-1)DPC or water(CK).Seven days after treatment,the Cry1Ac protein content,α-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content were measured,and transcriptome sequencing was performed.DESeq was used for differential gene analysis.Under the DPC treatment,the Cry1Ac protein content increased by 4.7-11.9% compared to CK.Theα-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content all increased.Transcriptome analysis revealed 7,542 upregulated genes and 10,449 downregulated genes for DPC vs.CK.Gene ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analyses showed that the differentially expressed genes were mainly involved in biological processes,such as carbon and amino acid metabolism.For example,genes encoding 6-phosphofructokinase,pyruvate kinase,glutamic pyruvate transaminase,pyruvate dehydrogenase,citrate synthase,isocitrate dehydrogenase,2-oxoglutarate dehydrogenase,glutamate synthase,1-pyrroline-5-carboxylate dehydrogenase,glutamic oxaloacetic transaminase,amino-acid N-acetyltransferase,and acetylornithine deacetylase were all significantly upregulated.The DPC treatment increased pyruvate,α-ketoglutarate,and oxaloacetate by increasing the operational rate of the glycolytic pathway of the citric acid cycle.It also significantly upregulated the genes encoding glutamate synthase,pyrrolidine-5-carboxylic acid dehydrogenase,glutamate oxaloacetate transaminase,and N-acetylglutamate synthetase,while it downregulated the genes encoding glutamine synthetase.Therefore,the synthesis of aspartic acid,glutamic acid,pyruvate,and arginine increased after treatment with DPC,and the Cry1Ac protein content was increased by regulating carbon and amino acid metabolism.
文摘火电机组磨煤机存在运行条件恶劣、故障频发等问题,对磨煤机进行故障预警,可以有效防止一些常见故障的发生,从而保证火电机组的安全运行。为此,提出一种基于相互邻近度的密度峰值聚类和多元状态估计的磨煤机故障预警方法。首先,采用核主元分析选取磨煤机的主要状态参数,同时采用集合经验模态分解对历史运行数据进行去噪,进一步优化数据质量;然后,采用基于相互邻近度的密度峰值聚类(density peaks clustering based on mutual neighborhood degrees,DPC-MND)方法构建动态记忆矩阵,利用多元状态估计技术(multivariate state estimation techniques,MSET)对磨煤机正常运行工况下的历史数据进行建模,并确定磨煤机的运行状态。最后,以安徽某电厂ZGM113G型中速磨煤机为例进行验证,结果表明该方法可以实现对磨煤机故障的有效预警。
