Detection of CEA mRNA in patients with non-small cell lung cancer and it’s significance

Objective: To detect the expression of CEA mRNA in patients with non-small cell lung cancer (NSCLC) and to investigate it's significance. Methods: The blood samples were taken from peripheral veins of 70 patients with NSCLC and 18 patients with benign diseases at 3 intervals during the surgery. The transcription of carcinoembryonic antigen messenger ribonucleic acid (CEA mRNA) was assayed by means of nested reverse transcriptase polymerase chain reaction (RT-PCR) and micro-fluid chip. Results: The CEA mRNA positive rates at each of the 3 time spots were as follows: 50% at beginning of the surgery (group 1), 62.8% in the samples collected when ligating the pulmonary vein (group 2) and 57.1% in samples collected 1 h after ligation (group 3). A significant difference was found between groups 1 and 2 (χ2 = 7.114, P < 0.05). Con-clusion: Cancer cell dissemination during surgery is demonstrated indirectly in our study, when to ligate the pulmonary vein (earlier or later) may affect the quantity of tumor cells spread into the circulation.

Electrically controlled mRNA delivery using a polypyrrolegraphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells

Direct messenger ribonucleic acid(mRNA)delivery to target cells or tissues has revolutionized the field of biotechnology.However,the applicability of regenerative medicine is limited by the technical difficulties of various mRNA-loaded nanocarriers.Herein,we report a new conductive hybrid film that could guide osteogenic differentiation of human adipose-derived mesenchymal stem cells(hADMSCs)via electrically controlled mRNA delivery.To find optimal electrical conductivity and mRNAloading capacity,the polypyrrole-graphene oxide(PPy-GO)hybrid film was electropolymerized on indium tin oxide substrates.We found that the fluorescein sodium salt,a molecule partially mimicking the physical and chemical properties of mRNAs,can be effectively absorbed and released by electrical stimulation(ES).The hADMSCs cultivated on the PPy-GO hybrid film loaded with pre-osteogenic mRNAs showed the highest osteogenic differentiation under electrical stimulation.This platform can load various types of RNAs thus highly promising as a new nucleic acid delivery tool for the development of stem cell-based therapeutics.