A Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using streptomyces sp.and pressmud as the substrate via solid-state fermentation(SSF).From the experiments,thr...A Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using streptomyces sp.and pressmud as the substrate via solid-state fermentation(SSF).From the experiments,three nutrients viz.yeast extract,FeSO_(4)·7H_(2)O,and NH4NO3 were found to be the most significant components.Hence these three components were selected and optimized using Response Surface Methodology(RSM).The optimum conditions are:yeast extract 0.00274 g/gds,FeSO_(4)·7H_(2)O 0.00011 g/gds and NH4NO30.00772 g/gds.The effect of the substrate concentration and initial moisture content were also studied.A substrate concentration of 12 g and an initial moisture content of 65%are optimum for the maximum production of inulinase(89 U/gds).展开更多
文摘A Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using streptomyces sp.and pressmud as the substrate via solid-state fermentation(SSF).From the experiments,three nutrients viz.yeast extract,FeSO_(4)·7H_(2)O,and NH4NO3 were found to be the most significant components.Hence these three components were selected and optimized using Response Surface Methodology(RSM).The optimum conditions are:yeast extract 0.00274 g/gds,FeSO_(4)·7H_(2)O 0.00011 g/gds and NH4NO30.00772 g/gds.The effect of the substrate concentration and initial moisture content were also studied.A substrate concentration of 12 g and an initial moisture content of 65%are optimum for the maximum production of inulinase(89 U/gds).
