In order to improve the accuracy and efficiency of 3D model retrieval,the method based on affinity propagation clustering algorithm is proposed. Firstly,projection ray-based method is proposed to improve the feature e...In order to improve the accuracy and efficiency of 3D model retrieval,the method based on affinity propagation clustering algorithm is proposed. Firstly,projection ray-based method is proposed to improve the feature extraction efficiency of 3D models. Based on the relationship between model and its projection,the intersection in 3D space is transformed into intersection in 2D space,which reduces the number of intersection and improves the efficiency of the extraction algorithm. In feature extraction,multi-layer spheres method is analyzed. The two-layer spheres method makes the feature vector more accurate and improves retrieval precision. Secondly,Semi-supervised Affinity Propagation ( S-AP) clustering is utilized because it can be applied to different cluster structures. The S-AP algorithm is adopted to find the center models and then the center model collection is built. During retrieval process,the collection is utilized to classify the query model into corresponding model base and then the most similar model is retrieved in the model base. Finally,75 sample models from Princeton library are selected to do the experiment and then 36 models are used for retrieval test. The results validate that the proposed method outperforms the original method and the retrieval precision and recall ratios are improved effectively.展开更多
The accurate equilibrium structures of S_3 and S_3^- are determined by the coupled-cluster method with single, double excitation and perturbative triple excitation(CCSD(T)) with basis sets of aug-cc-pV(n+d)Z(n = T, Q,...The accurate equilibrium structures of S_3 and S_3^- are determined by the coupled-cluster method with single, double excitation and perturbative triple excitation(CCSD(T)) with basis sets of aug-cc-pV(n+d)Z(n = T, Q, 5, or 6), complete basis set extrapolation functions with two-parameters and three-parameters, together with considering the contributions due to the core-valence electron correlation, scalar relativistic effects, spin–orbit coupling, and zero-point vibrational corrections. Our calculations show that both the neutral S_3 and anion S_3^- have open forms with C_(2r) vsymmetry. On the basis of the stable geometries, the adiabatic electron affinity of S_3 is determined to be 19041(11) cm^(-1), which is in excellent agreement with the experimental data(19059(7) cm^(-1)). The dependence of geometries and electron affinity on the computation level and physical corrections is discussed. The present computational results are helpful to the experimental molecular spectroscopy and bonding of S_3.展开更多
A novel control method for a general class of nonlinear systems using fuzzy logic systems (FLSs) is presertted. Indirect and direct methods are combined to design the adaptive fuzzy output feedback controller and a ...A novel control method for a general class of nonlinear systems using fuzzy logic systems (FLSs) is presertted. Indirect and direct methods are combined to design the adaptive fuzzy output feedback controller and a high-gain observer is used to estimate the derivatives of the system output. The closed-loop system is proven to be semiglobally uniformly ultimately bounded. In addition, it is shown that if the approximation accuracy of the fuzzy logic system is high enough and the observer gain is chosen sufficiently large, an arbitrarily small tracking error can be achieved. Simulation results verify the effectiveness of the newly designed scheme and the theoretical discussion.展开更多
[Objective]Crop line extraction is critical for improving the efficiency of autonomous agricultural machines in the field.However,traditional detection methods struggle to maintain high accuracy and efficiency under c...[Objective]Crop line extraction is critical for improving the efficiency of autonomous agricultural machines in the field.However,traditional detection methods struggle to maintain high accuracy and efficiency under challenging conditions,such as strong light exposure and weed interference.The aims are to develop an effective crop line extraction method by combining YOLOv8-G,Affinity Propagation,and the Least Squares method to enhance detection accuracy and performance in complex field environments.[Methods]The proposed method employs machine vision techniques to address common field challenges.YOLOv8-G,an improved object detection algorithm that combines YOLOv8 and Ghost‐NetV2 for lightweight,high-speed performance,was used to detect the central points of crops.These points were then clustered using the Affinity Propagation algorithm,followed by the application of the Least Squares method to extract the crop lines.Comparative tests were conducted to evaluate multiple backbone networks within the YOLOv8 framework,and ablation studies were performed to validate the enhancements made in YOLOv8-G.[Results and Discussions]The performance of the proposed method was compared with classical object detection and clustering algorithms.The YOLOv8-G algorithm achieved average precision(AP)values of 98.22%,98.15%,and 97.32%for corn detection at 7,14,and 21 days after emergence,respectively.Additionally,the crop line extraction accuracy across all stages was 96.52%.These results demonstrate the model's ability to maintain high detection accuracy despite challenging conditions in the field.[Conclusions]The proposed crop line extraction method effectively addresses field challenges such as lighting and weed interference,enabling rapid and accurate crop identification.This approach supports the automatic navigation of agricultural machinery,offering significant improvements in the precision and efficiency of field operations.展开更多
As we enter the year of 2011, the 2009 H1N1 pandemic influenza virus is in the news again. At least 20 people have died of this virus in China since the beginning of 2011 and it is now the predominant flu strain in th...As we enter the year of 2011, the 2009 H1N1 pandemic influenza virus is in the news again. At least 20 people have died of this virus in China since the beginning of 2011 and it is now the predominant flu strain in the country. Although this novel virus was quite stable during its run in the flu season of 2009-2010, a genetic variant of this virus was found in Singapore in early 2010, and then in Australia and New Zealand during their 2010 winter influenza season. Several critical mutations in the HA protein of this variant were uncovered in the strains collected from January 2010 to April 2010. Moreover, a structural homology model of HA from the A/Brisbane/10/2010(H1N1) strain was made based on the structure of A/California/04/2009 (H1N1). The purpose of this study was to investigate mutations in the HA protein of 2009 H1N1 from sequence data collected worldwide from May 2010 to February 2011. A fundamental problem in bioinformatics and biology is to find the similar gene sequences for a given gene sequence of interest. Here we proposed the inverse problem, i.e., finding the exemplars from a group of related gene sequences. With a clustering algorithm affinity propagation, six exemplars of the HA sequences were identified to represent six clusters. One of the clusters contained strain A/Brisbane/12/2010(H1N1) that only differed from A/Brisbane/10/2010 in the HA sequence at position 449. Based on the sequence identity of the six exemplars, nine mutations in HA were located that could be used to distinguish these six clusters. Finally, we discovered the change of correlation patterns for the HA and NA of 2009 H1N1 as a result of the HA receptor binding specificity switch, revealing the balanced interplay between these two surface proteins of the virus.展开更多
In this study, the lattice Boltzmann method (LBM) was used to simulate the solute transport in a single rough fracture. The self-affine rough fracture wall was generated with the successive random addition method. T...In this study, the lattice Boltzmann method (LBM) was used to simulate the solute transport in a single rough fracture. The self-affine rough fracture wall was generated with the successive random addition method. The ability of the developed LBM to simulate the solute transport was validated by Taylor dispersion. The effect of fluid velocity on the solute transport in a single rough fracture was investigated using the LBM. The breakthrough curves (BTCs) for continuous injection sources in rough fractures were analyzed and discussed with different Reynolds numbers (Re). The results show that the rough frac~'e wall leads to a large fluid velocity gradient across the aperture. Consequently, there is a broad distribution of the immobile region along the rough fracture wall. This distribution of the immobile region is very sensitive to the Re and fracture geometry, and the immobile region is enlarged with the increase of Re and roughness. The concentration of the solute front in the mobile region increases with the Re. Furthermore, the Re and roughness have significant effects on BTCs, and the slow solute molecule exchange between the mobile and immobile regions results in a long breakthrough tail for the rough fracture. This study also demonstrates that the developed LBM can be effective in studying the solute transport in a rough fracture.展开更多
In this paper, we propose an iterative relaxation method for solving the Hamilton-Jacobi-Bellman-Isaacs equation(HJBIE) arising in deterministic optimal control of affine nonlinear systems. Local convergence of the me...In this paper, we propose an iterative relaxation method for solving the Hamilton-Jacobi-Bellman-Isaacs equation(HJBIE) arising in deterministic optimal control of affine nonlinear systems. Local convergence of the method is established under fairly mild assumptions, and examples are solved to demonstrate the effectiveness of the method. An extension of the approach to Lyapunov equations is also discussed. The preliminary results presented are promising, and it is hoped that the approach will ultimately develop into an efficient computational tool for solving the HJBIEs.展开更多
In this paper, Pinellia ternata lectin (PTL) purified from 95% saturated ammonium sulfate precipitation by applying mannose-Sepharose 4B affinity column chromatography was first described. The mannose-Sepharose 4B aff...In this paper, Pinellia ternata lectin (PTL) purified from 95% saturated ammonium sulfate precipitation by applying mannose-Sepharose 4B affinity column chromatography was first described. The mannose-Sepharose 4B affinity adsorption gel has already been designed and prepared before the experiments according the combinative characterization between lectin and mannose. Mass spectrometry analysis result shows that PTL is a glycoprotein with the molecular weight of 12.165 kD. It was conclude that PTL had strong agglutination effects on mouse red blood cells, and the minimum reaction concentration was 25 μg/ml according the Hemagglutination. Results of automatic amino acid analysis indicated that PTL mainly contained 15 varieties of amino acids, of which the minimum content was cysteine and aspartate was the maximum. Cell experiment results suggested that PTL of low concentrations (0.004 mg/ml, 0.02 mg/ml and 0.1 mg/ml) promoted HeLa cell proliferation, but the effect weakened with the concentrations and treated-time increasing. However, the HeLa cells pro- liferation was intensively inhibited by higher PTL concentrations (0.5 mg/ml and 1 mg/ml), and the effect increased in a dose and time dependent manner.展开更多
Background The interaction of gas and liquid can produce many interesting phenomena,such as bubbles rising from the bottom of the liquid.The simulation of two-phase fluids is a challenging topic in computer graphics.T...Background The interaction of gas and liquid can produce many interesting phenomena,such as bubbles rising from the bottom of the liquid.The simulation of two-phase fluids is a challenging topic in computer graphics.To animate the interaction of a gas and liquid,MultiFLIP samples the two types of particles,and a Euler grid is used to track the interface of the liquid and gas.However,MultiFLIP uses the fluid implicit particle(FLIP)method to interpolate the velocities of particles into the Euler grid,which suffer from additional noise and instability.Methods To solve the problem caused by fluid implicit particles(FLIP),we present a novel velocity transport technique for two individual particles based on the affine particle-in-cell(APIC)method.First,we design a weighed coupling method for interpolating the velocities of liquid and gas particles to the Euler grid such that we can apply the APIC method to the simulation of a two-phase fluid.Second,we introduce a narrowband method to our system because MultiFLIP is a time-consuming approach owing to the large number of particles.Results Experiments show that our method is well integrated with the APIC method and provides a visually credible two-phase fluid animation.Conclusions The proposed method can successfully handle the simulation of a two phase fluid.展开更多
The measured profiles of laboratory fractured rocks should be self-affine fractal.The scaling properties of these profiles are described by two parameters-the fractal dimension D and the crossover length tc The D valu...The measured profiles of laboratory fractured rocks should be self-affine fractal.The scaling properties of these profiles are described by two parameters-the fractal dimension D and the crossover length tc The D values of eight profiles are calculated by the ruler method and by the standard deviation method respectively.It is shown that if tc is far greater than the sampling step tc two methods yield the same results,although if it is far smaller than r,the D by the standard method will be about 1.20,while D by the ruler method will very close to 1.0,because two fractal dimensions,local and global,exist on two sides of tc In order to obtain the local fractal dimension which may be close to that of the standard deviation method,the ruler method must be modified.We propose a way to estimate the tc and to modify the ruler method.Finally,a profile having given D is generated in terms of the principle of non-integer order differential,through which the above two methods are verified and lead to the same展开更多
核酸适配体是人工合成的短链核酸,作为分子识别元件,能够与各类靶标物质高特异性、高亲和力的结合,分为单链DNA和RNA两种类型。其中单链DNA适配体由于其稳定性比RNA适配体更好而更受欢迎,因此得到广泛应用。核酸适配体筛选通常是通过配...核酸适配体是人工合成的短链核酸,作为分子识别元件,能够与各类靶标物质高特异性、高亲和力的结合,分为单链DNA和RNA两种类型。其中单链DNA适配体由于其稳定性比RNA适配体更好而更受欢迎,因此得到广泛应用。核酸适配体筛选通常是通过配体指数富集系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)实现的,筛选能否成功在很大程度上取决于其最关键的单链制备步骤,即将双链DNA转化为相应的单链DNA。目前,存在许多方法可以制备单链DNA,包括热变性法、生物素-链霉亲和素亲和分离法、变性胶电泳分离法、核酸外切酶消化法、不对称聚合酶链式反应(polymerase chain reaction,PCR)法等。本文在总结文献报道的基础上,具体阐述了各种单链DNA制备方法的原理、优缺点及近5年的应用情况,并对这些单链DNA制备方法进行了比较和展望,以期能为成功筛选各类靶标的核酸适配体提供参考。展开更多
岩土颗粒材料的应变局部化失效问题广泛存在于工程设计应用中,主要表现为介观尺度上的应变局部化现象和宏观尺度上的剪切带产生,目前在微观尺度上的形成机理尚不明确。为了系统研究颗粒集合体的应变局部化的形成与演化过程,通过使用离...岩土颗粒材料的应变局部化失效问题广泛存在于工程设计应用中,主要表现为介观尺度上的应变局部化现象和宏观尺度上的剪切带产生,目前在微观尺度上的形成机理尚不明确。为了系统研究颗粒集合体的应变局部化的形成与演化过程,通过使用离散单元法(Discrete Element Method,DEM)模拟了指定平面应变加载路径的真三轴试验,获取了宏观和微观尺度上的颗粒材料几何、运动以及力学信息。为了找出表征应变局部化特性的最佳特征量,比较了颗粒温度、波动位移和局部剪胀角等微观指标,发现波动位移在表征应变局部化方面与其他参数相比相关性更好,并选定其作为应变局部化表征变量。为了量化颗粒集合体从应变局部化开始产生到发育完成的具体应变区间,采用莫兰指数对波动位移的空间分布特征进行统计和分析,确定了颗粒集合体在弹塑性转换阶段的发育区间。进一步对颗粒集合体应变局部化发育区间内的波动位移空间分布进行探究,并统计不同空间区域内波动位移的概率密度函数,发现研究区域外部的局部塑性在过渡阶段停止演化,而内部塑性以逾渗模式发展。最终,通过波动位移的空间分布进行聚类分析,获得了描述介观尺度上应变局部化的团簇体模型。该模型可以将颗粒集合体宏观剪切带的形成与发育和单个颗粒的微观塑性发展相联系,刻画颗粒集合体从介观尺度上的应变局部化出现到宏观尺度上剪切带完全形成的演化过程。展开更多
基金Sponsored by the National Natural Science Foundation of China (Grant No. 51075083)
文摘In order to improve the accuracy and efficiency of 3D model retrieval,the method based on affinity propagation clustering algorithm is proposed. Firstly,projection ray-based method is proposed to improve the feature extraction efficiency of 3D models. Based on the relationship between model and its projection,the intersection in 3D space is transformed into intersection in 2D space,which reduces the number of intersection and improves the efficiency of the extraction algorithm. In feature extraction,multi-layer spheres method is analyzed. The two-layer spheres method makes the feature vector more accurate and improves retrieval precision. Secondly,Semi-supervised Affinity Propagation ( S-AP) clustering is utilized because it can be applied to different cluster structures. The S-AP algorithm is adopted to find the center models and then the center model collection is built. During retrieval process,the collection is utilized to classify the query model into corresponding model base and then the most similar model is retrieved in the model base. Finally,75 sample models from Princeton library are selected to do the experiment and then 36 models are used for retrieval test. The results validate that the proposed method outperforms the original method and the retrieval precision and recall ratios are improved effectively.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11874179,11447194,11574114,and 11874177)the Natural Science Foundation of Jilin Province,China(Grant No.20180101289JC)
文摘The accurate equilibrium structures of S_3 and S_3^- are determined by the coupled-cluster method with single, double excitation and perturbative triple excitation(CCSD(T)) with basis sets of aug-cc-pV(n+d)Z(n = T, Q, 5, or 6), complete basis set extrapolation functions with two-parameters and three-parameters, together with considering the contributions due to the core-valence electron correlation, scalar relativistic effects, spin–orbit coupling, and zero-point vibrational corrections. Our calculations show that both the neutral S_3 and anion S_3^- have open forms with C_(2r) vsymmetry. On the basis of the stable geometries, the adiabatic electron affinity of S_3 is determined to be 19041(11) cm^(-1), which is in excellent agreement with the experimental data(19059(7) cm^(-1)). The dependence of geometries and electron affinity on the computation level and physical corrections is discussed. The present computational results are helpful to the experimental molecular spectroscopy and bonding of S_3.
基金This project was supported by the National Natural Science Foundation of China (90405011).
文摘A novel control method for a general class of nonlinear systems using fuzzy logic systems (FLSs) is presertted. Indirect and direct methods are combined to design the adaptive fuzzy output feedback controller and a high-gain observer is used to estimate the derivatives of the system output. The closed-loop system is proven to be semiglobally uniformly ultimately bounded. In addition, it is shown that if the approximation accuracy of the fuzzy logic system is high enough and the observer gain is chosen sufficiently large, an arbitrarily small tracking error can be achieved. Simulation results verify the effectiveness of the newly designed scheme and the theoretical discussion.
文摘[Objective]Crop line extraction is critical for improving the efficiency of autonomous agricultural machines in the field.However,traditional detection methods struggle to maintain high accuracy and efficiency under challenging conditions,such as strong light exposure and weed interference.The aims are to develop an effective crop line extraction method by combining YOLOv8-G,Affinity Propagation,and the Least Squares method to enhance detection accuracy and performance in complex field environments.[Methods]The proposed method employs machine vision techniques to address common field challenges.YOLOv8-G,an improved object detection algorithm that combines YOLOv8 and Ghost‐NetV2 for lightweight,high-speed performance,was used to detect the central points of crops.These points were then clustered using the Affinity Propagation algorithm,followed by the application of the Least Squares method to extract the crop lines.Comparative tests were conducted to evaluate multiple backbone networks within the YOLOv8 framework,and ablation studies were performed to validate the enhancements made in YOLOv8-G.[Results and Discussions]The performance of the proposed method was compared with classical object detection and clustering algorithms.The YOLOv8-G algorithm achieved average precision(AP)values of 98.22%,98.15%,and 97.32%for corn detection at 7,14,and 21 days after emergence,respectively.Additionally,the crop line extraction accuracy across all stages was 96.52%.These results demonstrate the model's ability to maintain high detection accuracy despite challenging conditions in the field.[Conclusions]The proposed crop line extraction method effectively addresses field challenges such as lighting and weed interference,enabling rapid and accurate crop identification.This approach supports the automatic navigation of agricultural machinery,offering significant improvements in the precision and efficiency of field operations.
文摘As we enter the year of 2011, the 2009 H1N1 pandemic influenza virus is in the news again. At least 20 people have died of this virus in China since the beginning of 2011 and it is now the predominant flu strain in the country. Although this novel virus was quite stable during its run in the flu season of 2009-2010, a genetic variant of this virus was found in Singapore in early 2010, and then in Australia and New Zealand during their 2010 winter influenza season. Several critical mutations in the HA protein of this variant were uncovered in the strains collected from January 2010 to April 2010. Moreover, a structural homology model of HA from the A/Brisbane/10/2010(H1N1) strain was made based on the structure of A/California/04/2009 (H1N1). The purpose of this study was to investigate mutations in the HA protein of 2009 H1N1 from sequence data collected worldwide from May 2010 to February 2011. A fundamental problem in bioinformatics and biology is to find the similar gene sequences for a given gene sequence of interest. Here we proposed the inverse problem, i.e., finding the exemplars from a group of related gene sequences. With a clustering algorithm affinity propagation, six exemplars of the HA sequences were identified to represent six clusters. One of the clusters contained strain A/Brisbane/12/2010(H1N1) that only differed from A/Brisbane/10/2010 in the HA sequence at position 449. Based on the sequence identity of the six exemplars, nine mutations in HA were located that could be used to distinguish these six clusters. Finally, we discovered the change of correlation patterns for the HA and NA of 2009 H1N1 as a result of the HA receptor binding specificity switch, revealing the balanced interplay between these two surface proteins of the virus.
基金supported by the National Natural Science Foundation of China(Grants No.51079043,41172204,and 51109139)the Natural Science Foundation of Jiangsu Province(Grant No.BK2011110)
文摘In this study, the lattice Boltzmann method (LBM) was used to simulate the solute transport in a single rough fracture. The self-affine rough fracture wall was generated with the successive random addition method. The ability of the developed LBM to simulate the solute transport was validated by Taylor dispersion. The effect of fluid velocity on the solute transport in a single rough fracture was investigated using the LBM. The breakthrough curves (BTCs) for continuous injection sources in rough fractures were analyzed and discussed with different Reynolds numbers (Re). The results show that the rough frac~'e wall leads to a large fluid velocity gradient across the aperture. Consequently, there is a broad distribution of the immobile region along the rough fracture wall. This distribution of the immobile region is very sensitive to the Re and fracture geometry, and the immobile region is enlarged with the increase of Re and roughness. The concentration of the solute front in the mobile region increases with the Re. Furthermore, the Re and roughness have significant effects on BTCs, and the slow solute molecule exchange between the mobile and immobile regions results in a long breakthrough tail for the rough fracture. This study also demonstrates that the developed LBM can be effective in studying the solute transport in a rough fracture.
文摘In this paper, we propose an iterative relaxation method for solving the Hamilton-Jacobi-Bellman-Isaacs equation(HJBIE) arising in deterministic optimal control of affine nonlinear systems. Local convergence of the method is established under fairly mild assumptions, and examples are solved to demonstrate the effectiveness of the method. An extension of the approach to Lyapunov equations is also discussed. The preliminary results presented are promising, and it is hoped that the approach will ultimately develop into an efficient computational tool for solving the HJBIEs.
文摘In this paper, Pinellia ternata lectin (PTL) purified from 95% saturated ammonium sulfate precipitation by applying mannose-Sepharose 4B affinity column chromatography was first described. The mannose-Sepharose 4B affinity adsorption gel has already been designed and prepared before the experiments according the combinative characterization between lectin and mannose. Mass spectrometry analysis result shows that PTL is a glycoprotein with the molecular weight of 12.165 kD. It was conclude that PTL had strong agglutination effects on mouse red blood cells, and the minimum reaction concentration was 25 μg/ml according the Hemagglutination. Results of automatic amino acid analysis indicated that PTL mainly contained 15 varieties of amino acids, of which the minimum content was cysteine and aspartate was the maximum. Cell experiment results suggested that PTL of low concentrations (0.004 mg/ml, 0.02 mg/ml and 0.1 mg/ml) promoted HeLa cell proliferation, but the effect weakened with the concentrations and treated-time increasing. However, the HeLa cells pro- liferation was intensively inhibited by higher PTL concentrations (0.5 mg/ml and 1 mg/ml), and the effect increased in a dose and time dependent manner.
基金National High Technology R&D Program of China(2017YFB1002701,M2019YFB1600702)NSFC(62072449)+1 种基金Science and Technology Development Fund,Macao SAR(0018/2019/AKP,0008/2019/AGJ,SKL-IOTSC-2018-2020)University of Macao Grant(MYRG2019-00006-FST).
文摘Background The interaction of gas and liquid can produce many interesting phenomena,such as bubbles rising from the bottom of the liquid.The simulation of two-phase fluids is a challenging topic in computer graphics.To animate the interaction of a gas and liquid,MultiFLIP samples the two types of particles,and a Euler grid is used to track the interface of the liquid and gas.However,MultiFLIP uses the fluid implicit particle(FLIP)method to interpolate the velocities of particles into the Euler grid,which suffer from additional noise and instability.Methods To solve the problem caused by fluid implicit particles(FLIP),we present a novel velocity transport technique for two individual particles based on the affine particle-in-cell(APIC)method.First,we design a weighed coupling method for interpolating the velocities of liquid and gas particles to the Euler grid such that we can apply the APIC method to the simulation of a two-phase fluid.Second,we introduce a narrowband method to our system because MultiFLIP is a time-consuming approach owing to the large number of particles.Results Experiments show that our method is well integrated with the APIC method and provides a visually credible two-phase fluid animation.Conclusions The proposed method can successfully handle the simulation of a two phase fluid.
基金This work has been supported by the National Natural Science Foundation of China under Grant No. 10471051, and also by the Key Project of Chinese National Programs for Fundamental Research and Development(973 program) through Grant No. 2004CB318000, and also by the Fundamental Research Funds for the Central Universities under Grant No. M2009027.
文摘The measured profiles of laboratory fractured rocks should be self-affine fractal.The scaling properties of these profiles are described by two parameters-the fractal dimension D and the crossover length tc The D values of eight profiles are calculated by the ruler method and by the standard deviation method respectively.It is shown that if tc is far greater than the sampling step tc two methods yield the same results,although if it is far smaller than r,the D by the standard method will be about 1.20,while D by the ruler method will very close to 1.0,because two fractal dimensions,local and global,exist on two sides of tc In order to obtain the local fractal dimension which may be close to that of the standard deviation method,the ruler method must be modified.We propose a way to estimate the tc and to modify the ruler method.Finally,a profile having given D is generated in terms of the principle of non-integer order differential,through which the above two methods are verified and lead to the same
文摘核酸适配体是人工合成的短链核酸,作为分子识别元件,能够与各类靶标物质高特异性、高亲和力的结合,分为单链DNA和RNA两种类型。其中单链DNA适配体由于其稳定性比RNA适配体更好而更受欢迎,因此得到广泛应用。核酸适配体筛选通常是通过配体指数富集系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)实现的,筛选能否成功在很大程度上取决于其最关键的单链制备步骤,即将双链DNA转化为相应的单链DNA。目前,存在许多方法可以制备单链DNA,包括热变性法、生物素-链霉亲和素亲和分离法、变性胶电泳分离法、核酸外切酶消化法、不对称聚合酶链式反应(polymerase chain reaction,PCR)法等。本文在总结文献报道的基础上,具体阐述了各种单链DNA制备方法的原理、优缺点及近5年的应用情况,并对这些单链DNA制备方法进行了比较和展望,以期能为成功筛选各类靶标的核酸适配体提供参考。
文摘岩土颗粒材料的应变局部化失效问题广泛存在于工程设计应用中,主要表现为介观尺度上的应变局部化现象和宏观尺度上的剪切带产生,目前在微观尺度上的形成机理尚不明确。为了系统研究颗粒集合体的应变局部化的形成与演化过程,通过使用离散单元法(Discrete Element Method,DEM)模拟了指定平面应变加载路径的真三轴试验,获取了宏观和微观尺度上的颗粒材料几何、运动以及力学信息。为了找出表征应变局部化特性的最佳特征量,比较了颗粒温度、波动位移和局部剪胀角等微观指标,发现波动位移在表征应变局部化方面与其他参数相比相关性更好,并选定其作为应变局部化表征变量。为了量化颗粒集合体从应变局部化开始产生到发育完成的具体应变区间,采用莫兰指数对波动位移的空间分布特征进行统计和分析,确定了颗粒集合体在弹塑性转换阶段的发育区间。进一步对颗粒集合体应变局部化发育区间内的波动位移空间分布进行探究,并统计不同空间区域内波动位移的概率密度函数,发现研究区域外部的局部塑性在过渡阶段停止演化,而内部塑性以逾渗模式发展。最终,通过波动位移的空间分布进行聚类分析,获得了描述介观尺度上应变局部化的团簇体模型。该模型可以将颗粒集合体宏观剪切带的形成与发育和单个颗粒的微观塑性发展相联系,刻画颗粒集合体从介观尺度上的应变局部化出现到宏观尺度上剪切带完全形成的演化过程。