To investigate a contribution of O-methylguanineto mutagenesis in mouse cells,we constructed ashuttle vector plasmid,pYZ289,from a part ofpZ189 plasmid and polyoma virus DNA.The plasmidcontains a supF gene as a marker...To investigate a contribution of O-methylguanineto mutagenesis in mouse cells,we constructed ashuttle vector plasmid,pYZ289,from a part ofpZ189 plasmid and polyoma virus DNA.The plasmidcontains a supF gene as a marker of mutation andcan replicate in both E.coli and mouse cells.ThepYZ289 treated with N-methyl-N-nitrosourea (MNU)展开更多
1-Methy-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）is a neurotoxin that selectively damages dopaminergic neurons in the substantia nigra pars compacta and induces Parkinson＇s like symptoms in rodents.Quercetin（QC）i...1-Methy-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）is a neurotoxin that selectively damages dopaminergic neurons in the substantia nigra pars compacta and induces Parkinson＇s like symptoms in rodents.Quercetin（QC）is a natural polyphenolic bioflavonoid with potent antioxidant and anti-inflammatory properties but lacks of clinical attraction due to low oral bioavailability.Piperine is a well established bioavailability enhancer used pre-clinically to improve the bioavailability of antioxidants（e.g.,Quercetin）.Therefore,the present study was designed to evaluate the neuroprotective potential of QC together with piperine against MPTP-induced neurotoxicity in rats.MPTP（100μg/μL/rat,bilaterally）was injected intranigrally on days 1,4 and 7 using a digital stereotaxic apparatus.QC（25 and 50 mg/kg,intragastrically）and QC（25 mg/kg,intragastrically）in combination with piperine（2.5 mg/kg,intragastrically）were administered daily for 14 days starting from day 8 after the 3^（rd） injection of MPTP.On day 22,animals were sacrificed and the striatum was isolated for oxidative stress parameter（thiobarbituric acid reactive substances,nitrite and glutathione）,neuroinflammatory cytokine（interleukin-1β,interleukin-6,and tumor necrosis factor-α）and neurotransmitter（dopamine,norepinephrine,serotonin,gamma-aminobutyric acid,glutamate,3,4-dihydroxyphenylacetic acid,homovanillic acid,and 5-hydroxyindoleacetic acid）evaluations.Bilateral infusion of MPTP into substantia nigra pars compacta led to significant motor deficits as evidenced by impairments in locomotor activity and rotarod performance in open field test and grip strength and narrow beam walk performance.Both QC（25 and 50 mg/kg）and QC（25 mg/kg）in combination with piperine（2.5 mg/kg）,in particular the combination therapy,significantly improved MPTP-induced behavioral abnormalities in rats,reversed the abnormal alterations of neurotransmitters in the striatum,and alleviated oxidative stress and inflammatory response in the striatum.These findings indicate that piperine can enhance the antioxidant and anti-inflammatory properties of QC,and QC in combination with piperine exhibits strong neuroprotective effects against MPTP-induced neurotoxicity.展开更多
When we try to talk about civilisation exchanges and mutual learning between China and Bulgaria,it's easy and difficult.China boasts the most ancient civilisation in the world with an ever-lasting history of over ...When we try to talk about civilisation exchanges and mutual learning between China and Bulgaria,it's easy and difficult.China boasts the most ancient civilisation in the world with an ever-lasting history of over 5000 years.With a history of over 1300 years,Bulgaria is one of the oldest civilisations in Europe.There are three major civilisations emerging and thriving on this very land.展开更多
An Essential Proposal Exactly a year ago,Chinese President Xi Jinping put forward the Global Civilisation Initiative(GCl).In his speech addressing a dialogue between the Communist Party of China and other world politi...An Essential Proposal Exactly a year ago,Chinese President Xi Jinping put forward the Global Civilisation Initiative(GCl).In his speech addressing a dialogue between the Communist Party of China and other world political parties via video link in March 2023,Xi explained why he proposed the GCl:"As the future of all countries is closely connected,tolerance,coexistence,exchanges and mutual learning among different civilisations play an irreplaceable role in advancing humanity's modernisation process,and making the garden of world civilisation flourish."展开更多
Aimed at studying normali zed radial basis function network (NRBFN), this paper introduces the subtractiv e clustering based on a mountain function to construct the initial structure of NR BFN, adopts singular value ...Aimed at studying normali zed radial basis function network (NRBFN), this paper introduces the subtractiv e clustering based on a mountain function to construct the initial structure of NR BFN, adopts singular value decomposition (SVD) to analyze the relationship betwe en neural nodes of the hidden layer and singular values, cumulative contribution ratio, index vector, and optimizes the structure of NRBFN. Finally, simulation and performance comparison show that the algorithm is feasible and effective.展开更多
The yield characters and quality characters of several new cotton varieties in the mid-south area of Hebei Province were comprehensively evaluated in this pa- per. In different ecological environments, the single-boll...The yield characters and quality characters of several new cotton varieties in the mid-south area of Hebei Province were comprehensively evaluated in this pa- per. In different ecological environments, the single-boll weight of tested cotton vari- eties had the biggest variation coefficient among yield characters and micronaire value had the biggest variation coefficient among fiber quality characters. Path analy- sis indicated that the direct path coefficients of various characters had the flowing order, lint percentage〉 number of bolls per plant〉 single-boll weight〉 seed index among yield components and uniformity index 〉 fiber specific strength〉 micronaire value〉 elongation rate〉 upper half staple length among fiber quality characters. Principal component analysis indicated that the top three principal components contributed 8.263% of accumulated variance, basically reflecting the vast majority of informations of 10 main characters in tested cotton varieties.展开更多
The preparation of ultra fine Y A G( Y3 Al5 O12) po w der through co precipitation techniquew as investigated . The for m ation tem perature of Y A G w as lo wered belo w 800 ℃ by carefullycontrolling process...The preparation of ultra fine Y A G( Y3 Al5 O12) po w der through co precipitation techniquew as investigated . The for m ation tem perature of Y A G w as lo wered belo w 800 ℃ by carefullycontrolling process para m eters . Ultra fine Y A G po w der w ith even size distribution , spheri cal shapes , and 40 n m particle size has been produced . It is found that for m ation of Y A Gisnot through the Y A M an d Y A Pinter mediate phases that are often observed during the synthe sis of Y A G by m ixed oxide m ethod . The sm all particle size of the precursor po w der , them olecular scale mixing of the constituents , and the poly m orphic transfor m ation of Al2 O3during heating accou nt m ainly for the reduction of for m ation tem perature of Y A Gan d the e lim ination of inter m ediate Y A M and Y A Pphases .展开更多
The paper gives two estimates of the distance between adjacent zeros of solutions of the first\|order delay differential equation x′(t)+p(t)x(t-τ) =0 in the case when p(t)≥0 and ∫ t t-τ p(s)d s-1e ...The paper gives two estimates of the distance between adjacent zeros of solutions of the first\|order delay differential equation x′(t)+p(t)x(t-τ) =0 in the case when p(t)≥0 and ∫ t t-τ p(s)d s-1e oscillates or p(t) itself oscillates.展开更多
From the constitutive model with generalized force fields for a viscoelastic body with damage, the differential equations of motion for thin and thick plates with damage are derived under arbitrary boundary conditions...From the constitutive model with generalized force fields for a viscoelastic body with damage, the differential equations of motion for thin and thick plates with damage are derived under arbitrary boundary conditions. The convolution-type functionals for the bending of viscoelastic thin and thick plates with damage are presented, and the corresponding generalized variational principles are given. From these generalized principles, all the basic equations of the displacement and damage variables and initial and boundary conditions can be deduced. As an example, we compare the difference between the dynamical properties of plates with and without damage and consider the effect of damage on the dynamical properties of plates.展开更多
The cutting performance of particle reinforced meta ll ic matrix composites (PRMMCs) SiC p/Al in ultrasonic vibration cutting and comm on cutting with carbide tools and PCD tools was experimentally researched in the p...The cutting performance of particle reinforced meta ll ic matrix composites (PRMMCs) SiC p/Al in ultrasonic vibration cutting and comm on cutting with carbide tools and PCD tools was experimentally researched in the paper. The changing rules of chip shape, deformation coefficient, shear angle a nd surface residual stress were presented by ultrasonic vibration cutting. Resul ts show: when adopting common cutting, spiral chip with smaller curl radius will be obtained. The chip with zigzag contour is short and thick. There are lots of sheet cracking both on the face of the chip and on the machined surface. That i s to say, the cutting process of metallic matrix composites(MMCs) is not all lik e the cutting process of plastic material. It is akin to the breaking process of brittle material. By comparison, when adopting ultrasonic cutting, because tool contacts with workpiece intermittently in high frequency, deformation of chip i s small, loose spiral chip with larger curl radius is long and thin. The phenome non is just similar to vibration cutting of plastic material. But the chip still belongs to plastic or semi-plastic segmental chip due to the structure charact eristics of the material itself. Furthermore, the tangential residual compressio n stress of vibration cutting is larger than that of common cutting, axial resid ual stress has a relation to the feed rate and residual stress does not changes obviously with cutting depth and they are in the same order of magnitude on the whole. According to the test result analyzing, the following conclusions are put forward: 1) The extruding deformation is serious in common cutting PRMMCs, defo rmation of it’s chip is larger, and the chip with lesser curl radius is short. Whereas, the deformation of chip in vibration cutting PRMMCs is lesser, the curl radius is bigger, and the loose chips are obtained at every turn. 2) The cuttin g deformation coefficient of chip in vibration cutting is lesser than that in co mmon cutting, however the shear angle is bigger. 3) The tangential residual compression stress of vibration cutting is larger than that of common cutting, a nd residual stress does not change obviously with cutting depth, they are in the same order of magnitude on the whole.展开更多
The molecular mechanism of DNA damage induced by hydroquinone （HQ） remains unclear. Poly（ADP-ribose） polymerase-1 （PARP-1） usually works as a DNA damage sensor, and hence, it is possible that PARP-1 is involved ...The molecular mechanism of DNA damage induced by hydroquinone （HQ） remains unclear. Poly（ADP-ribose） polymerase-1 （PARP-1） usually works as a DNA damage sensor, and hence, it is possible that PARP-1 is involved in the DNA damage response induced by HQ. In TK6 cells treated with HQ, PARP activity as well as the expression of apoptosis antagonizing transcription factor （AATF）, PARP-1, and phosphorylated H2AX （v-H2AX） were maximum at 0.5 h, 6 h, 3 h, and 3 h, respectively. To explore the detailed mechanisms underlying the prompt DNA repair reaction, the above indicators were investigated in PARP-l-silenced cells. PARP activity and expression of AATF and PARP-1 decreased to 36%, 32%, and 33%, respectively, in the cells; however, y-H2AX expression increased to 265%. Co-immunoprecipitation （co-IP） assays were employed to determine whether PARP-1 and AATF formed protein complexes. The interaction between these proteins together with the results from IP assays and confocal microscopy indicated that poly（ADP-ribosyl）ation {PARylation） regulated AATF expression, in conclusion, PARP-1 was involved in the DNA damage repair induced by HQ via increasing the accumulation of AATF through PARylation.展开更多
Recent studies showed that inflammation is a critical cause for initiation and/or development of many cancers. In prostate cancer(PC), the inflammatory cells usually populate an immune-competent organ. This inflammato...Recent studies showed that inflammation is a critical cause for initiation and/or development of many cancers. In prostate cancer(PC), the inflammatory cells usually populate an immune-competent organ. This inflammatory organ can be involved in the initiation and progression of PC. Here, we mainly focus on the role of inflammation in the PC and progression of castration-resistant PC(CRPC). Moreover, we summarize the roles of inflammation factors(such as chemokines and cytokines) in PC and CRPC. Taken together, this review gives an insight into therapy for PC and CRPC through anti-inflammation.展开更多
As to solve the online monitoring of the inner temperatur e and freezing profile of the reaction shaft of flash smelting furnace, simulation of the wall in the reaction shaft in a flash smelting furnace was made throu...As to solve the online monitoring of the inner temperatur e and freezing profile of the reaction shaft of flash smelting furnace, simulation of the wall in the reaction shaft in a flash smelting furnace was made through numerical computation. The computational results are very near the data got in s ite. The error of the moving boundary is approximately 3%, and that of the tempe rature is less than 5%. It is proved that the simulation software is applicable for practice to monitor the temperature and moving boundary inside the hearth on line. Based on a large number of the data computed, the relation between the cha nge of the moving boundary and inner temperature is summarized, and the great in fluence of the cooling system on the forming and stability of the moving boundar y inside the hearth is emphasized, which provide the theoretical bases for optim izing the flash smelting operation.展开更多
文摘To investigate a contribution of O-methylguanineto mutagenesis in mouse cells,we constructed ashuttle vector plasmid,pYZ289,from a part ofpZ189 plasmid and polyoma virus DNA.The plasmidcontains a supF gene as a marker of mutation andcan replicate in both E.coli and mouse cells.ThepYZ289 treated with N-methyl-N-nitrosourea (MNU)
基金Science and Engineering Board(SERB),Department of Science and Technology,Government of India,New Delhi for providing financial assistance under Fast Track Scheme(DST-SERB-FTYS)(SB/FT/LS-139/2012) to Dr.PK
文摘1-Methy-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）is a neurotoxin that selectively damages dopaminergic neurons in the substantia nigra pars compacta and induces Parkinson＇s like symptoms in rodents.Quercetin（QC）is a natural polyphenolic bioflavonoid with potent antioxidant and anti-inflammatory properties but lacks of clinical attraction due to low oral bioavailability.Piperine is a well established bioavailability enhancer used pre-clinically to improve the bioavailability of antioxidants（e.g.,Quercetin）.Therefore,the present study was designed to evaluate the neuroprotective potential of QC together with piperine against MPTP-induced neurotoxicity in rats.MPTP（100μg/μL/rat,bilaterally）was injected intranigrally on days 1,4 and 7 using a digital stereotaxic apparatus.QC（25 and 50 mg/kg,intragastrically）and QC（25 mg/kg,intragastrically）in combination with piperine（2.5 mg/kg,intragastrically）were administered daily for 14 days starting from day 8 after the 3^（rd） injection of MPTP.On day 22,animals were sacrificed and the striatum was isolated for oxidative stress parameter（thiobarbituric acid reactive substances,nitrite and glutathione）,neuroinflammatory cytokine（interleukin-1β,interleukin-6,and tumor necrosis factor-α）and neurotransmitter（dopamine,norepinephrine,serotonin,gamma-aminobutyric acid,glutamate,3,4-dihydroxyphenylacetic acid,homovanillic acid,and 5-hydroxyindoleacetic acid）evaluations.Bilateral infusion of MPTP into substantia nigra pars compacta led to significant motor deficits as evidenced by impairments in locomotor activity and rotarod performance in open field test and grip strength and narrow beam walk performance.Both QC（25 and 50 mg/kg）and QC（25 mg/kg）in combination with piperine（2.5 mg/kg）,in particular the combination therapy,significantly improved MPTP-induced behavioral abnormalities in rats,reversed the abnormal alterations of neurotransmitters in the striatum,and alleviated oxidative stress and inflammatory response in the striatum.These findings indicate that piperine can enhance the antioxidant and anti-inflammatory properties of QC,and QC in combination with piperine exhibits strong neuroprotective effects against MPTP-induced neurotoxicity.
文摘When we try to talk about civilisation exchanges and mutual learning between China and Bulgaria,it's easy and difficult.China boasts the most ancient civilisation in the world with an ever-lasting history of over 5000 years.With a history of over 1300 years,Bulgaria is one of the oldest civilisations in Europe.There are three major civilisations emerging and thriving on this very land.
文摘An Essential Proposal Exactly a year ago,Chinese President Xi Jinping put forward the Global Civilisation Initiative(GCl).In his speech addressing a dialogue between the Communist Party of China and other world political parties via video link in March 2023,Xi explained why he proposed the GCl:"As the future of all countries is closely connected,tolerance,coexistence,exchanges and mutual learning among different civilisations play an irreplaceable role in advancing humanity's modernisation process,and making the garden of world civilisation flourish."
文摘Aimed at studying normali zed radial basis function network (NRBFN), this paper introduces the subtractiv e clustering based on a mountain function to construct the initial structure of NR BFN, adopts singular value decomposition (SVD) to analyze the relationship betwe en neural nodes of the hidden layer and singular values, cumulative contribution ratio, index vector, and optimizes the structure of NRBFN. Finally, simulation and performance comparison show that the algorithm is feasible and effective.
基金Supported by Project of Cotton Industry Technology System in Hebei of National Science-technology Support Plan(2009BADA3B01)~~
文摘The yield characters and quality characters of several new cotton varieties in the mid-south area of Hebei Province were comprehensively evaluated in this pa- per. In different ecological environments, the single-boll weight of tested cotton vari- eties had the biggest variation coefficient among yield characters and micronaire value had the biggest variation coefficient among fiber quality characters. Path analy- sis indicated that the direct path coefficients of various characters had the flowing order, lint percentage〉 number of bolls per plant〉 single-boll weight〉 seed index among yield components and uniformity index 〉 fiber specific strength〉 micronaire value〉 elongation rate〉 upper half staple length among fiber quality characters. Principal component analysis indicated that the top three principal components contributed 8.263% of accumulated variance, basically reflecting the vast majority of informations of 10 main characters in tested cotton varieties.
文摘The preparation of ultra fine Y A G( Y3 Al5 O12) po w der through co precipitation techniquew as investigated . The for m ation tem perature of Y A G w as lo wered belo w 800 ℃ by carefullycontrolling process para m eters . Ultra fine Y A G po w der w ith even size distribution , spheri cal shapes , and 40 n m particle size has been produced . It is found that for m ation of Y A Gisnot through the Y A M an d Y A Pinter mediate phases that are often observed during the synthe sis of Y A G by m ixed oxide m ethod . The sm all particle size of the precursor po w der , them olecular scale mixing of the constituents , and the poly m orphic transfor m ation of Al2 O3during heating accou nt m ainly for the reduction of for m ation tem perature of Y A Gan d the e lim ination of inter m ediate Y A M and Y A Pphases .
文摘The paper gives two estimates of the distance between adjacent zeros of solutions of the first\|order delay differential equation x′(t)+p(t)x(t-τ) =0 in the case when p(t)≥0 and ∫ t t-τ p(s)d s-1e oscillates or p(t) itself oscillates.
基金Project supported by the National Natural Sciences Foundation of China (No. 10272069) the Shanghai Key Subject Program.
文摘From the constitutive model with generalized force fields for a viscoelastic body with damage, the differential equations of motion for thin and thick plates with damage are derived under arbitrary boundary conditions. The convolution-type functionals for the bending of viscoelastic thin and thick plates with damage are presented, and the corresponding generalized variational principles are given. From these generalized principles, all the basic equations of the displacement and damage variables and initial and boundary conditions can be deduced. As an example, we compare the difference between the dynamical properties of plates with and without damage and consider the effect of damage on the dynamical properties of plates.
文摘The cutting performance of particle reinforced meta ll ic matrix composites (PRMMCs) SiC p/Al in ultrasonic vibration cutting and comm on cutting with carbide tools and PCD tools was experimentally researched in the paper. The changing rules of chip shape, deformation coefficient, shear angle a nd surface residual stress were presented by ultrasonic vibration cutting. Resul ts show: when adopting common cutting, spiral chip with smaller curl radius will be obtained. The chip with zigzag contour is short and thick. There are lots of sheet cracking both on the face of the chip and on the machined surface. That i s to say, the cutting process of metallic matrix composites(MMCs) is not all lik e the cutting process of plastic material. It is akin to the breaking process of brittle material. By comparison, when adopting ultrasonic cutting, because tool contacts with workpiece intermittently in high frequency, deformation of chip i s small, loose spiral chip with larger curl radius is long and thin. The phenome non is just similar to vibration cutting of plastic material. But the chip still belongs to plastic or semi-plastic segmental chip due to the structure charact eristics of the material itself. Furthermore, the tangential residual compressio n stress of vibration cutting is larger than that of common cutting, axial resid ual stress has a relation to the feed rate and residual stress does not changes obviously with cutting depth and they are in the same order of magnitude on the whole. According to the test result analyzing, the following conclusions are put forward: 1) The extruding deformation is serious in common cutting PRMMCs, defo rmation of it’s chip is larger, and the chip with lesser curl radius is short. Whereas, the deformation of chip in vibration cutting PRMMCs is lesser, the curl radius is bigger, and the loose chips are obtained at every turn. 2) The cuttin g deformation coefficient of chip in vibration cutting is lesser than that in co mmon cutting, however the shear angle is bigger. 3) The tangential residual compression stress of vibration cutting is larger than that of common cutting, a nd residual stress does not change obviously with cutting depth, they are in the same order of magnitude on the whole.
基金supported by grants from the National Natural Science Foundation of China(8120223181273116+2 种基金81430079)the Science and Technology Program of Guangdong Bureau of Science and TechnologyChina(2013B021800069)
文摘The molecular mechanism of DNA damage induced by hydroquinone （HQ） remains unclear. Poly（ADP-ribose） polymerase-1 （PARP-1） usually works as a DNA damage sensor, and hence, it is possible that PARP-1 is involved in the DNA damage response induced by HQ. In TK6 cells treated with HQ, PARP activity as well as the expression of apoptosis antagonizing transcription factor （AATF）, PARP-1, and phosphorylated H2AX （v-H2AX） were maximum at 0.5 h, 6 h, 3 h, and 3 h, respectively. To explore the detailed mechanisms underlying the prompt DNA repair reaction, the above indicators were investigated in PARP-l-silenced cells. PARP activity and expression of AATF and PARP-1 decreased to 36%, 32%, and 33%, respectively, in the cells; however, y-H2AX expression increased to 265%. Co-immunoprecipitation （co-IP） assays were employed to determine whether PARP-1 and AATF formed protein complexes. The interaction between these proteins together with the results from IP assays and confocal microscopy indicated that poly（ADP-ribosyl）ation {PARylation） regulated AATF expression, in conclusion, PARP-1 was involved in the DNA damage repair induced by HQ via increasing the accumulation of AATF through PARylation.
文摘Recent studies showed that inflammation is a critical cause for initiation and/or development of many cancers. In prostate cancer(PC), the inflammatory cells usually populate an immune-competent organ. This inflammatory organ can be involved in the initiation and progression of PC. Here, we mainly focus on the role of inflammation in the PC and progression of castration-resistant PC(CRPC). Moreover, we summarize the roles of inflammation factors(such as chemokines and cytokines) in PC and CRPC. Taken together, this review gives an insight into therapy for PC and CRPC through anti-inflammation.
文摘As to solve the online monitoring of the inner temperatur e and freezing profile of the reaction shaft of flash smelting furnace, simulation of the wall in the reaction shaft in a flash smelting furnace was made through numerical computation. The computational results are very near the data got in s ite. The error of the moving boundary is approximately 3%, and that of the tempe rature is less than 5%. It is proved that the simulation software is applicable for practice to monitor the temperature and moving boundary inside the hearth on line. Based on a large number of the data computed, the relation between the cha nge of the moving boundary and inner temperature is summarized, and the great in fluence of the cooling system on the forming and stability of the moving boundar y inside the hearth is emphasized, which provide the theoretical bases for optim izing the flash smelting operation.
