AIM: To determine the possible association of the ApoB100 (Xba Ⅰ ), ApoE (Hha Ⅰ ) and CYP7A1 (Bsa Ⅰ ) gene polymorphisms, with the development of cholesterol gallstone disease (GD) in a Mexican population. METHODS:...AIM: To determine the possible association of the ApoB100 (Xba Ⅰ ), ApoE (Hha Ⅰ ) and CYP7A1 (Bsa Ⅰ ) gene polymorphisms, with the development of cholesterol gallstone disease (GD) in a Mexican population. METHODS: The polymorphisms were analyzed by polymerase chain reaction followed by restriction fragment length polymorphism, in two groups matched by ethnicity, age and sex: patients with GD (n = 101) and stone-free control subjects (n = 101). RESULTS: Allelic frequencies in patients and controls were: 34.16% vs 41.58% (P = 0.124) for X+of ApoB-100; 4.46% vs 5.94% (P = 0.501) for E2, 85.64% vs 78.22% (P = 0.052) for E3, 9.90% vs 15.84% (P = 0.075) for E4 of ApoE; and 25.74% vs 27.72% (P = 0.653) for C of CYP7A1. Differences in genotypic frequencies between the studied groups were not significant (P < 0.05). CONCLUSION: These results demonstrated that no association exists between the studied polymorphisms and cholelithiasis in this high prevalent population.展开更多
Background: CYP2C19 is a major isoform of cytochrome P450 that metabolizes a number of commonly prescribed drugs such as omeprazole, diazepam, tolbutamide and propranolol. Its expression is regulated by the constituti...Background: CYP2C19 is a major isoform of cytochrome P450 that metabolizes a number of commonly prescribed drugs such as omeprazole, diazepam, tolbutamide and propranolol. Its expression is regulated by the constitutive androstane receptor (CAR), involved in glucocorticoids synthesis. Since a number of crossliniks have been described for CYPs and some hormones, an association of CYP2C19 with type 2 diabetes is likely. Methods: Two groups were studied, 352 diagnosed with type 2 diabetes patients and 342 healthy volunteers form Mexico City. Both groups were tested for CYP2C19*2 and *3 alleles. We carried out an allelic discrimination using TaqMan assay for *2, and used FRET sensor and anchor probes for *3. Results: Ninety one percent of the subjects had the wild type allele, 9% have the *2 allele;no subject presented the *3 allele. The CYP2C19*2 allele is associated with type 2 diabetes (p = 0.012). Admixmap program was used to correct the admixture of this population and get the correlation. This was further confirmed in a linear model with a 67% power and by the method of Strom and Wienker for association on subjects within the mean range of Amerindian ancestry only (60%). Conclusion: Type 2 diabetes patients have significatly more *2 allele than healthy volunteers, more evident for the patients with the homocygous genotype.展开更多
Individual variations in the fat mass and obesity-associated (FTO) gene have been associated with obesity and BMI in diverse populations, but there are no reports in young Mexicans. We explored the association of a co...Individual variations in the fat mass and obesity-associated (FTO) gene have been associated with obesity and BMI in diverse populations, but there are no reports in young Mexicans. We explored the association of a common FTO single-nucleotide polymorphism (SNP, rs805704) with obesity-related phenotypes in Mexican young adults. The FTO-SNP was genotyped using the fluorescent polarization method in college-age, apparently healthy subjects from the “UP AMIGOS” cohort (n = 251, 18 - 25 yrs). Homozygotes for the A allele (15%, n = 38) were heavier (1.6 kg/m2 and6.2 kg) and had a larger waist circumference (WC,4.8 cm) than G allele carriers. The FTO genotype was associated with BMI, weight and WC independently of age and sex and explained 2.7 to 3.1% of the variance in obesity-related phenotypes. The FTO genotype was also associated with fasting glucose (P = 0.0283). No other associations were found in the additive model. Despite our small sample size, we found that the FTO-rs805704 genotype influences obesity-related phenotypes young Mexicans. Previously observed FTO associations with fasting glucose were replicated. Previously reported associations with other metabolic traits likely represent the long-term consequences of obesity.展开更多
Erectile dysfunction is treated with 5-phospodiesterase inhibitors as Mirodenafil, which has shown its efficacy and safety in Koreans, however;no information in other populations is available. An open clinical trial s...Erectile dysfunction is treated with 5-phospodiesterase inhibitors as Mirodenafil, which has shown its efficacy and safety in Koreans, however;no information in other populations is available. An open clinical trial study was designed to evaluate the efficacy and safety in real life of a fixed-dose of Mirodenafil in Mexican patients with erectile dysfunction. Forty-seven male patients received a 100 mg tablet of Mirodenafil, during 12 weeks. Primary outcome efficacy measure was the percentage of male patients with successful intercourse. Secondary outcomes measures included patient satisfaction, mood and self-esteem level. Safety assessments included laboratory tests, vital signs, physical examination, 12-lead electrocardiogram recordings, and incidence of adverse events by patients. Oral administration of Mirodenafil improved in an 80% - 90% the number of successful intercourses from 7 to 84 days of treatment. Moreover, patients reported a significant increment in their sexual satisfaction, mood and self-esteem. Mirodenafil treatment did not modify vital signs nor anthropometric parameters during 84 days. Mild headache was the most frequent adverse event (17.0%) and there were no severe adverse events during pharmacological treatment. Data suggest that oral Mirodenafil is safety, well tolerated and effective in the Mexican population with erectile dysfunction.展开更多
Growing evidence indicates that the Asian monsoon plays an important role in affecting the weather and climate outside of Asia. However, this active role of the monsoon has not been demonstrated as thoroughly as has t...Growing evidence indicates that the Asian monsoon plays an important role in affecting the weather and climate outside of Asia. However, this active role of the monsoon has not been demonstrated as thoroughly as has the variability of the monsoon caused by various impacting factors such as sea surface temperature and land surface. This study investigates the relationship between the Asian monsoon and the climate anomalies in the Asian-Pacific-American （APA） sector. A hypothesis is tested that the variability of the upper-tropospheric South Asian high （SAH）, which is closely associated with the overall heating of the large-scale Asian monsoon, is linked to changes in the subtropical western Pacific high （SWPH）, the midPacific trough, and the Mexican high. The changes in these circulation systems cause variability in surface temperature and precipitation in the APA region. A stronger SAH is accompanied by a stronger and more extensive SWPH. The enlargement of the SWPH weakens the mid-Pacific trough. As a result, the southern portion of the Mexican high becomes stronger. These changes are associated with changes in atmospheric teleconnections, precipitation, and surface temperature throughout the APA region. When the SAH is stronger, precipitation increases in southern Asia, decreases over the Pacific Ocean, and increases over the Central America. Precipitation also increases over Australia and central Africa and decreases in the Mediterranean region. While the signals in surface temperature are weak over the tropical land portion, they are apparent in the mid latitudes and over the eastern Pacific Ocean.展开更多
Objective: To determinate the recovery of total polyphenolic compounds content,in vitro antioxidant activity and HPLC/ESI/MS characterization of extract from Nephelium lappaceum L.(Mexican rambutan).Methods: The rambu...Objective: To determinate the recovery of total polyphenolic compounds content,in vitro antioxidant activity and HPLC/ESI/MS characterization of extract from Nephelium lappaceum L.(Mexican rambutan).Methods: The rambutan husk extract was obtained by aqueous extraction and a polyphenolic fraction was recovered using Amberlite XAD-16. The total polyphenolic compounds content was determined by the Folin Ciocalteu and butanol-HCI methods.In vitro antioxidant activity was performed using ABTS and ferric reducing antioxidant power methods.Results: Mexican rambutan husk showed a total polyphenolic content of 582 mg/g and an evident antioxidant activity by ABTS and ferric reducing antioxidant power analysis.The HPLC/ESI/MS assay allowed the identification of 13 compounds, most of which belong to ellagitannins. Geraniin, corilagin and ellagic acid were present in the sample;the mineral composition was also evaluated.Conclusions: Rambutan husk cultivated in Mexico is a promising source for the recovery of added value bioactive compounds with antioxidant activity, which have potential applications as bioactive antioxidant agents for the treatment of diseases.展开更多
基金Supported by PROFAPI-UAS and CECYT from Sinaloa, México
文摘AIM: To determine the possible association of the ApoB100 (Xba Ⅰ ), ApoE (Hha Ⅰ ) and CYP7A1 (Bsa Ⅰ ) gene polymorphisms, with the development of cholesterol gallstone disease (GD) in a Mexican population. METHODS: The polymorphisms were analyzed by polymerase chain reaction followed by restriction fragment length polymorphism, in two groups matched by ethnicity, age and sex: patients with GD (n = 101) and stone-free control subjects (n = 101). RESULTS: Allelic frequencies in patients and controls were: 34.16% vs 41.58% (P = 0.124) for X+of ApoB-100; 4.46% vs 5.94% (P = 0.501) for E2, 85.64% vs 78.22% (P = 0.052) for E3, 9.90% vs 15.84% (P = 0.075) for E4 of ApoE; and 25.74% vs 27.72% (P = 0.653) for C of CYP7A1. Differences in genotypic frequencies between the studied groups were not significant (P < 0.05). CONCLUSION: These results demonstrated that no association exists between the studied polymorphisms and cholelithiasis in this high prevalent population.
文摘Background: CYP2C19 is a major isoform of cytochrome P450 that metabolizes a number of commonly prescribed drugs such as omeprazole, diazepam, tolbutamide and propranolol. Its expression is regulated by the constitutive androstane receptor (CAR), involved in glucocorticoids synthesis. Since a number of crossliniks have been described for CYPs and some hormones, an association of CYP2C19 with type 2 diabetes is likely. Methods: Two groups were studied, 352 diagnosed with type 2 diabetes patients and 342 healthy volunteers form Mexico City. Both groups were tested for CYP2C19*2 and *3 alleles. We carried out an allelic discrimination using TaqMan assay for *2, and used FRET sensor and anchor probes for *3. Results: Ninety one percent of the subjects had the wild type allele, 9% have the *2 allele;no subject presented the *3 allele. The CYP2C19*2 allele is associated with type 2 diabetes (p = 0.012). Admixmap program was used to correct the admixture of this population and get the correlation. This was further confirmed in a linear model with a 67% power and by the method of Strom and Wienker for association on subjects within the mean range of Amerindian ancestry only (60%). Conclusion: Type 2 diabetes patients have significatly more *2 allele than healthy volunteers, more evident for the patients with the homocygous genotype.
文摘Individual variations in the fat mass and obesity-associated (FTO) gene have been associated with obesity and BMI in diverse populations, but there are no reports in young Mexicans. We explored the association of a common FTO single-nucleotide polymorphism (SNP, rs805704) with obesity-related phenotypes in Mexican young adults. The FTO-SNP was genotyped using the fluorescent polarization method in college-age, apparently healthy subjects from the “UP AMIGOS” cohort (n = 251, 18 - 25 yrs). Homozygotes for the A allele (15%, n = 38) were heavier (1.6 kg/m2 and6.2 kg) and had a larger waist circumference (WC,4.8 cm) than G allele carriers. The FTO genotype was associated with BMI, weight and WC independently of age and sex and explained 2.7 to 3.1% of the variance in obesity-related phenotypes. The FTO genotype was also associated with fasting glucose (P = 0.0283). No other associations were found in the additive model. Despite our small sample size, we found that the FTO-rs805704 genotype influences obesity-related phenotypes young Mexicans. Previously observed FTO associations with fasting glucose were replicated. Previously reported associations with other metabolic traits likely represent the long-term consequences of obesity.
文摘Erectile dysfunction is treated with 5-phospodiesterase inhibitors as Mirodenafil, which has shown its efficacy and safety in Koreans, however;no information in other populations is available. An open clinical trial study was designed to evaluate the efficacy and safety in real life of a fixed-dose of Mirodenafil in Mexican patients with erectile dysfunction. Forty-seven male patients received a 100 mg tablet of Mirodenafil, during 12 weeks. Primary outcome efficacy measure was the percentage of male patients with successful intercourse. Secondary outcomes measures included patient satisfaction, mood and self-esteem level. Safety assessments included laboratory tests, vital signs, physical examination, 12-lead electrocardiogram recordings, and incidence of adverse events by patients. Oral administration of Mirodenafil improved in an 80% - 90% the number of successful intercourses from 7 to 84 days of treatment. Moreover, patients reported a significant increment in their sexual satisfaction, mood and self-esteem. Mirodenafil treatment did not modify vital signs nor anthropometric parameters during 84 days. Mild headache was the most frequent adverse event (17.0%) and there were no severe adverse events during pharmacological treatment. Data suggest that oral Mirodenafil is safety, well tolerated and effective in the Mexican population with erectile dysfunction.
文摘Growing evidence indicates that the Asian monsoon plays an important role in affecting the weather and climate outside of Asia. However, this active role of the monsoon has not been demonstrated as thoroughly as has the variability of the monsoon caused by various impacting factors such as sea surface temperature and land surface. This study investigates the relationship between the Asian monsoon and the climate anomalies in the Asian-Pacific-American （APA） sector. A hypothesis is tested that the variability of the upper-tropospheric South Asian high （SAH）, which is closely associated with the overall heating of the large-scale Asian monsoon, is linked to changes in the subtropical western Pacific high （SWPH）, the midPacific trough, and the Mexican high. The changes in these circulation systems cause variability in surface temperature and precipitation in the APA region. A stronger SAH is accompanied by a stronger and more extensive SWPH. The enlargement of the SWPH weakens the mid-Pacific trough. As a result, the southern portion of the Mexican high becomes stronger. These changes are associated with changes in atmospheric teleconnections, precipitation, and surface temperature throughout the APA region. When the SAH is stronger, precipitation increases in southern Asia, decreases over the Pacific Ocean, and increases over the Central America. Precipitation also increases over Australia and central Africa and decreases in the Mediterranean region. While the signals in surface temperature are weak over the tropical land portion, they are apparent in the mid latitudes and over the eastern Pacific Ocean.
文摘Objective: To determinate the recovery of total polyphenolic compounds content,in vitro antioxidant activity and HPLC/ESI/MS characterization of extract from Nephelium lappaceum L.(Mexican rambutan).Methods: The rambutan husk extract was obtained by aqueous extraction and a polyphenolic fraction was recovered using Amberlite XAD-16. The total polyphenolic compounds content was determined by the Folin Ciocalteu and butanol-HCI methods.In vitro antioxidant activity was performed using ABTS and ferric reducing antioxidant power methods.Results: Mexican rambutan husk showed a total polyphenolic content of 582 mg/g and an evident antioxidant activity by ABTS and ferric reducing antioxidant power analysis.The HPLC/ESI/MS assay allowed the identification of 13 compounds, most of which belong to ellagitannins. Geraniin, corilagin and ellagic acid were present in the sample;the mineral composition was also evaluated.Conclusions: Rambutan husk cultivated in Mexico is a promising source for the recovery of added value bioactive compounds with antioxidant activity, which have potential applications as bioactive antioxidant agents for the treatment of diseases.
