The uppermost Jurassic to Lower Cretaceous in the Qomolongma (Mt. Everest) area were deposited in a strong subsiding setting on passive continental margin. From Tithonian to Valanginian, the area experienced an enviro...The uppermost Jurassic to Lower Cretaceous in the Qomolongma (Mt. Everest) area were deposited in a strong subsiding setting on passive continental margin. From Tithonian to Valanginian, the area experienced an environment shift from circum\|shelf margin carbonate platform to foreslope, then to deep continental slope [1] . Correspondingly, the sediments change in vertical succession from quartz sandstone, via carbonate grainstone to dark shale intercalated with lithic sandstone of gravity flow. From the Tithonian to Valanginian, eight 3rd\|order sequences have been recognized [1] with clearly delineated sequence boundaries and reasonable biostratigraphic controls of ammonites. From the angle of sequence stratigraphy and sedimentary cycles, the important change happened at ca. 138Ma [2] of the Lower Tithonian (about 120m above Aulacosphinctoides hybonotum zone), where a waved subaerial erosion surface occurs in massive bioclastic grainstone. The boundary at ca. 134 5Ma (latest Tithonian, about 60m below the FAD of \%Berriasella cf. jacobi [3] ) comes to the next. The third one is at ca 132Ma (some 170m above the FAD of Berriasella cf. jacobi [3] , but about 60m below the FAD of Berriasella cf. grandis [3] ). In terms of sea\|level change, the sequence boundary at 134 5Ma is probably the most distinctive one, above which some 60m gravel\|bearing massive medium\|grained quartz sandstone was laid down and obviously truncates the underlying strata. In the dark shale below the sandstone, abundant in ammonites, such as Virgatosphinctes, Aulacosphinctes, Haplophylloceras and Gymnosphinctes are recorded [3] . The quartz sandstone itself does not bear identifiable fossils. Above the quartz sandstone, the strata mainly consists of dark silty shale up to 180m thick, with an prominent marine transgression at the base. Just above the first flooding surface (FFS), quite a number of ammonites such as \%Berriasella\% cf. \%jacobi, Blanofordiceras, Hymalayaites\% and \%Pseudosubplanites \%are documented [3] . The third sequence, which is delineated by submarine truncation of slope fan complexes at bottom, is also characterized by dark shale and silts, yielding ammonites \%Berriasella \%cf. \%grandis\% and B. cf. \%berthei.\%>From the viewpoint of sequence stratigraphy, we herein suggest to take the FFS in the second sequence as the J/K boundary. The sequence boundary below it can be used as a nice auxiliary marker, which represents a large sea\|level fall, and can be correlated widely in the world. From the angle of paleontology, the FFS is also ideal for the boundary, where the most important faunal turnover happened. Theoretically, taking FFS as a chronostratigraphic boundary has more advantages than using sequence boundary.展开更多
[Objective] To isolate three strains of avian leukosis virus subgroup J（ALV -J） , and then amplify and sequence the gp85 gene. [ Method] Three strains of ALV- J were isolated from Hubei Province, which were identifi...[Objective] To isolate three strains of avian leukosis virus subgroup J（ALV -J） , and then amplify and sequence the gp85 gene. [ Method] Three strains of ALV- J were isolated from Hubei Province, which were identified by pathological anatomy, DF- 1 cell culture and RT- PCR. And then they were named HB1002, HB1003 and HB1009, respectively. [ Result] Test sequence analysis showed, the length of gp85 gene was 921 bp, consistent with expect result; the nucleotide homology between the three isolates was in 97.7% -99.7% ,the homology of amino acid was in 95.1% - 99%. The nucleotide homology between HPRS - 103 and the three isolates was in the 94.1% - 94.8% ; and the nucleotide homolo- gy between other ALV -J and the three isolates was in 87.6% -97.3%. The phylogenetic trees analysis showed that the homology of JS09GY6 vi- rus and the three isolates was nearest, in 95.2% -97.3%. [ Conclusion] In the test, the three strains of virus which were isolated were ALV- J.展开更多
A subgroup J avian leukosis virus (AVL-J), designated as ZH-08, was isolated from a breeder flock in Guangdong province with a novel hemangioma case. The identification results of ELISA test, PCR and immunofluoresecen...A subgroup J avian leukosis virus (AVL-J), designated as ZH-08, was isolated from a breeder flock in Guangdong province with a novel hemangioma case. The identification results of ELISA test, PCR and immunofluoresecence assay (IFA) specific for ALV-J were all positive. Based on the public full-length proviral genome sequence of ALV-J prototype strain HPRS-103, three pairs of primers were synthesized. The full-length proviral genome sequence of ZH-08 isolate is 7597 bp, which has a little difference with that of published full-length genome sequences, but its organization corresponds with typical retroviral genome structure; and known oncogenes were not included in its genome. According to the gp85 sequence comparison of ZH-08 isolate with those of the other reference strains in China and abroad, the highest similarity (93.7%) was with the YZ9901 isolate. Phylogenetic analysis, based on the gp85 gene, showed that the ZH-08 isolated here had the closest linkage to the SD07LK1 isolate. This study provides the basis for the biological characterization and pathogenesis research of the ZH-08 isolate.展开更多
The Jayachamarajapura schist belt in western Dharwar craton, southern India, is predominantly an ultramafics dominant terrain. These rocks have been extensively metamorphosed and altered to serpentinite. The komatiite...The Jayachamarajapura schist belt in western Dharwar craton, southern India, is predominantly an ultramafics dominant terrain. These rocks have been extensively metamorphosed and altered to serpentinite. The komatiite nature of ultramafics is conspicuous. In most of the areas of the belt these ultramafics are massive in nature. However, some of the ultramafic units show layered nature. But, their outcrops are encompassed within the massive komatiitic bodies. These komatiitic ultramafics are predominantly Mg-rich in nature. The layered rocks are also Mg-rich, and their field setting and geochemistry suggest their intermittent occurrence as sills, during the differentiation of peridotitic magma. The layered rocks, which have been intensely serpentinisation show homogenous nature. They are almost wholly made of serpentine with occasional relics of pyroxene. Secondary carbonate mineral is often noticed. Their higher MgO content indicates Mg-rich ultramafic magmatism during Archaean orogeny.展开更多
基金theNationalNaturalScienceFoundationofChina (No ..4982 5 10 2 )
文摘The uppermost Jurassic to Lower Cretaceous in the Qomolongma (Mt. Everest) area were deposited in a strong subsiding setting on passive continental margin. From Tithonian to Valanginian, the area experienced an environment shift from circum\|shelf margin carbonate platform to foreslope, then to deep continental slope [1] . Correspondingly, the sediments change in vertical succession from quartz sandstone, via carbonate grainstone to dark shale intercalated with lithic sandstone of gravity flow. From the Tithonian to Valanginian, eight 3rd\|order sequences have been recognized [1] with clearly delineated sequence boundaries and reasonable biostratigraphic controls of ammonites. From the angle of sequence stratigraphy and sedimentary cycles, the important change happened at ca. 138Ma [2] of the Lower Tithonian (about 120m above Aulacosphinctoides hybonotum zone), where a waved subaerial erosion surface occurs in massive bioclastic grainstone. The boundary at ca. 134 5Ma (latest Tithonian, about 60m below the FAD of \%Berriasella cf. jacobi [3] ) comes to the next. The third one is at ca 132Ma (some 170m above the FAD of Berriasella cf. jacobi [3] , but about 60m below the FAD of Berriasella cf. grandis [3] ). In terms of sea\|level change, the sequence boundary at 134 5Ma is probably the most distinctive one, above which some 60m gravel\|bearing massive medium\|grained quartz sandstone was laid down and obviously truncates the underlying strata. In the dark shale below the sandstone, abundant in ammonites, such as Virgatosphinctes, Aulacosphinctes, Haplophylloceras and Gymnosphinctes are recorded [3] . The quartz sandstone itself does not bear identifiable fossils. Above the quartz sandstone, the strata mainly consists of dark silty shale up to 180m thick, with an prominent marine transgression at the base. Just above the first flooding surface (FFS), quite a number of ammonites such as \%Berriasella\% cf. \%jacobi, Blanofordiceras, Hymalayaites\% and \%Pseudosubplanites \%are documented [3] . The third sequence, which is delineated by submarine truncation of slope fan complexes at bottom, is also characterized by dark shale and silts, yielding ammonites \%Berriasella \%cf. \%grandis\% and B. cf. \%berthei.\%>From the viewpoint of sequence stratigraphy, we herein suggest to take the FFS in the second sequence as the J/K boundary. The sequence boundary below it can be used as a nice auxiliary marker, which represents a large sea\|level fall, and can be correlated widely in the world. From the angle of paleontology, the FFS is also ideal for the boundary, where the most important faunal turnover happened. Theoretically, taking FFS as a chronostratigraphic boundary has more advantages than using sequence boundary.
基金Plan of Hubei Province Science and Technology Research and Development Project:the Research and Demonstration of Comprehensive Prevention and Control Technology on Poultry Avian Influenza and other Major Diseases YJN0065the Construction Fund of Modern Agriculture Industry Technology System CARS-42-G11
文摘[Objective] To isolate three strains of avian leukosis virus subgroup J（ALV -J） , and then amplify and sequence the gp85 gene. [ Method] Three strains of ALV- J were isolated from Hubei Province, which were identified by pathological anatomy, DF- 1 cell culture and RT- PCR. And then they were named HB1002, HB1003 and HB1009, respectively. [ Result] Test sequence analysis showed, the length of gp85 gene was 921 bp, consistent with expect result; the nucleotide homology between the three isolates was in 97.7% -99.7% ,the homology of amino acid was in 95.1% - 99%. The nucleotide homology between HPRS - 103 and the three isolates was in the 94.1% - 94.8% ; and the nucleotide homolo- gy between other ALV -J and the three isolates was in 87.6% -97.3%. The phylogenetic trees analysis showed that the homology of JS09GY6 vi- rus and the three isolates was nearest, in 95.2% -97.3%. [ Conclusion] In the test, the three strains of virus which were isolated were ALV- J.
基金supported by National Science Foundation of Guangdong Province(Grant No.8151064201000065)Special Fund for Agro-scientific Research in the Public Interest(200803019)to Weisheng Cao+2 种基金NSFC-Guangdong Union Foundation(GrantNo.U0831002)National Natural Science Foundation(Grant No.30771612)Key Program of Science and Technology Development of Guangdong Province(Grant No.2009A020101006)to Ming Liao
文摘A subgroup J avian leukosis virus (AVL-J), designated as ZH-08, was isolated from a breeder flock in Guangdong province with a novel hemangioma case. The identification results of ELISA test, PCR and immunofluoresecence assay (IFA) specific for ALV-J were all positive. Based on the public full-length proviral genome sequence of ALV-J prototype strain HPRS-103, three pairs of primers were synthesized. The full-length proviral genome sequence of ZH-08 isolate is 7597 bp, which has a little difference with that of published full-length genome sequences, but its organization corresponds with typical retroviral genome structure; and known oncogenes were not included in its genome. According to the gp85 sequence comparison of ZH-08 isolate with those of the other reference strains in China and abroad, the highest similarity (93.7%) was with the YZ9901 isolate. Phylogenetic analysis, based on the gp85 gene, showed that the ZH-08 isolated here had the closest linkage to the SD07LK1 isolate. This study provides the basis for the biological characterization and pathogenesis research of the ZH-08 isolate.
文摘The Jayachamarajapura schist belt in western Dharwar craton, southern India, is predominantly an ultramafics dominant terrain. These rocks have been extensively metamorphosed and altered to serpentinite. The komatiite nature of ultramafics is conspicuous. In most of the areas of the belt these ultramafics are massive in nature. However, some of the ultramafic units show layered nature. But, their outcrops are encompassed within the massive komatiitic bodies. These komatiitic ultramafics are predominantly Mg-rich in nature. The layered rocks are also Mg-rich, and their field setting and geochemistry suggest their intermittent occurrence as sills, during the differentiation of peridotitic magma. The layered rocks, which have been intensely serpentinisation show homogenous nature. They are almost wholly made of serpentine with occasional relics of pyroxene. Secondary carbonate mineral is often noticed. Their higher MgO content indicates Mg-rich ultramafic magmatism during Archaean orogeny.
