Experimental study of enhanced in-situ micro-ecological remediation of petroleum contaminated loess soil was carried out in Zhongyuan oil production areas, and the enhanced in-situ micro-ecological remediation techniq...Experimental study of enhanced in-situ micro-ecological remediation of petroleum contaminated loess soil was carried out in Zhongyuan oil production areas, and the enhanced in-situ micro-ecological remediation technique includes optimistic in-situ microbial communities, physical chemistry methods, alfalfa planting and regulation of soil environmental elements. Experiments showed that the oil content in the contaminated soil with oil content about 2 898.25 mg/kg can be reduced about 98.61% after in-situ micro-ecological remediation for 99 days, which demonstrated the effectiveness of in-situ micro-ecological remediation methods for petroleum contaminated soil in central plains of China, and explored the practical and feasible application of these methods.展开更多
Probiotics are a kind of living microorganisms added into food or drug,which are beneficial to our health.Probiotics can regulate the balance of microecosystem in intestine.Recently,people have paid more attention to ...Probiotics are a kind of living microorganisms added into food or drug,which are beneficial to our health.Probiotics can regulate the balance of microecosystem in intestine.Recently,people have paid more attention to the effect on the intestinal microecology.This article mainly made a review on the mechanism and application of probiotics.展开更多
OBJECTIVE: To observe the effects of Zhenren Yangzang Decoction combined with modified Shenling Baizhu Powder on the immune function and intestinal microecology of diarrhea patients with deficiency and cold syndrome. ...OBJECTIVE: To observe the effects of Zhenren Yangzang Decoction combined with modified Shenling Baizhu Powder on the immune function and intestinal microecology of diarrhea patients with deficiency and cold syndrome. METHODS: A total of 60 diarrhea patients with deficiency and cold syndrome were randomly divided into observation group and control group, with 30 cases in each group. The control group was treated with conventional western medicine. The observation group was given the treatment of Zhenren Yangzang Decoction combined with modified Shenling Baizhu Powder on the basis of the same treatment for 4 weeks. The clinical symptoms, fecal flora and immunoglobulin content of the 2 groups were observed and the clinical efficacy was evaluated. RESULTS: After treatment, the symptoms scores of fecal diarrhea, aversion to cold, cold limbs, loss of appetite, cold and painful waist and knee, abdominal fullness, abdominal distension and abdominal pain, and intestinal bacilli contents were significantly decreased(P < 0.05). The contents of lactobacillus, bifidus bacilli, IgG, IgM and IgA were significantly increased(P < 0.05), and the improvement of the above indexes in the observation group was significantly better than that in the control group(P < 0.05). The total effective rate after treatment in the observation group was 93.3%, which was significantly higher than 73.3% of the control group(P < 0.05). CONCLUSION: The treatment of diarrhea patients with deficiency and cold syndrome with Zhenren Yangzang Decoction and modified Shenling Baizhu Powder can effectively improve the intestinal micro-ecological environment, improve the immune function of patients and promote the rapid recovery of patients.展开更多
The OLIGO+ Project has been launched by PNDC last year further to the observation that the micro-ecology imbalance status in China is responsible for sub-health.Taking the challenge to improve the public health and nu...The OLIGO+ Project has been launched by PNDC last year further to the observation that the micro-ecology imbalance status in China is responsible for sub-health.Taking the challenge to improve the public health and nutrition,OLIGO+ Project is aiming to raise the issue of micro ecology imbalance to a public level and advocate the addition of Oligo prebiotics in food by food industry.Consumption of prebiotics ingredient will then promote micro-ecological balance and public health.After this first year of successful implementation,OLIGO+ Project will keep the same ambitious objectives for 2009.The soluble fibers,mostly resistant to digestion in the small intestine and largely fermented in the colon demonstrating prebiotic effects have also been included in the OLIGO+ Project.展开更多
Mud crab is an important economic crab cultured in Pacific and Indian Ocean Rim. Based on the knowledge about the characteristic requirements in seedling cultivation of mud crab under high-temperature conditions, this...Mud crab is an important economic crab cultured in Pacific and Indian Ocean Rim. Based on the knowledge about the characteristic requirements in seedling cultivation of mud crab under high-temperature conditions, this article reviews and discusses major factors affecting seedling rearing of mud crabs, and presents novel hatchery facilities for improvement of seedling rearing. The results of our study showed that major factors affecting seedling cultivation of mud crabs included： （1） the micro-ecological environment and characteristics of water quality in seedling rearing ponds, and a double-bottom cultivation facilities and cultivation techniques that meet the requirement; （2） salinity, temperature, light, NOE-N, NH4-N and other physical and chemical environmental factors that affect survival probability ofzoaea; （3） the total number of bacteria in the zoaea-culturing pond and the number of vibrio in seedling rearing; and （4） nutritional intensification with bait organisms in zoaea-culturing. To meet and improve these conditions, a novel hatchery facility was designed to modify the pond roof in accordance with the characteristics of the zoaea stage, and improve natural light thermostat control in seedling cultivation of mud crabs.展开更多
It has been proposed that the intestinal microbiota and gastrointestinal tumors are interdependent.Changes in the microbiota can cause dysfunction of the gastrointestinal tract,thereby promoting carcinogenic changes,l...It has been proposed that the intestinal microbiota and gastrointestinal tumors are interdependent.Changes in the microbiota can cause dysfunction of the gastrointestinal tract,thereby promoting carcinogenic changes,leading to the occurrence of gastrointestinal tumors.Recent studies on intestinal microbiota have opened up a new area in intestinal micro-ecological immunotherapy.The intestinal microbiota is a double-edged sword.Gut microbes participate in carcinogenesis,but can also be used for immunotherapy.The intestinal microbiota is also regulated by the daily diet.Intestinal micro-ecological immunotherapy combines intestinal immune nutrition and intestinal ecological nutrition to make full use of the intestinal microbiota to strengthen nutritional support.Micro-ecological immunotherapy enhances the body’s immune function by providing energy,improving the functional state of tissues and organs,protecting the intestinal mucosal barrier and maintaining normal intestinal microbiota balance.This involves,to some extent,PD-1 and PD-L1.The microbiota is beneficial to improve the clinical efficacy of conventional anti-cancer therapy and to reduce the incidence of complications.At the same time,micro-ecological immunotherapy is itself active and effective in the perioperative treatment of tumors,which is of great significance for the prognosis of the patient.Gastrointestinal tumors are increasingly linked to intestinal microbiota,and various microbiota-related technologies and drugs have been developed.In the future,the intestinal microbiota may represent a screening marker for gastrointestinal tumors.In addition,clinicians may be able to prevent and treat cancers by changing the gene expression levels of certain microbiota,or by regulating the types of microbes present.展开更多
In-situ oral delivery of therapeutic antibodies,like monoclonal antibody,for chronic inflammation treatment is the most convenient approach compared with other administration routes.Moreover,the abundant links between...In-situ oral delivery of therapeutic antibodies,like monoclonal antibody,for chronic inflammation treatment is the most convenient approach compared with other administration routes.Moreover,the abundant links between the gut microbiota and colonic inflammation indicate that the synergistic or antagonistic effect of gut microbiota to colonic inflammation.However,the antibody activity would be significantly affected while transferring through the gastrointestinal tract due to hostile conditions.Moreover,these antibodies have short serum half-lives,thus,require to be frequently administered with high doses to be effective,leading to low patient tolerance.Here,we develop a strategy utilizing thin shell hydrogel microcapsule fabricated by microfluidic technique as the oral delivering carrier.By encapsulating antibodies in these microcapsules,antibodies survive in the hostile gastrointestinal environment and rapidly release into the small intestine through oral administration route,achieving the same therapeutic effect as the intravenous injection evaluated by a colonic inflammation disease model.Moreover,the abundance of some intestinal microorganisms as the indication of the improvement of inflammation has remarkably altered after in-situ antibody-laden microcapsules delivery,implying the restoration of micro-ecology of the intestine.These findings prove our microcapsules are exploited as an efficient oral delivery agent for antibodies with programmable function in clinical application.展开更多
Objective： To investigate the mechanism of the Chinese medicine theory that Fei（Lung） and Dachang（Large Intestine） are exteriorly and interiorly related via synchronous observation on the dynamic changes of the r...Objective： To investigate the mechanism of the Chinese medicine theory that Fei（Lung） and Dachang（Large Intestine） are exteriorly and interiorly related via synchronous observation on the dynamic changes of the respiratory and intestinal microflora. Methods： Forty specific pathogen free Sprague-Dawley rats were selected and randomly divided into blank（10 rats） and chronic bronchitis model groups（30 rats）. The blank group rats were put into the smoke-free environment and the model group rats were put into the smoke environment in order to establish pulmonary disease（chronic bronchitis） model. Then the corresponding changes of the respiratory and intestinal microflora of the model on 20th, 50th and 70th days were synchronously observed. Results： The respiratory tract microflora showed an increase in the total aerobic and Staphylococcus aureus and reduced anaerobic amount significantly on 20th day in the respiratory tract microflora（P〈0.05 or 0.01）. On 50th day, total aerobic, total anaerobic amount and bifidobacterium significantly increased（P〈0.05）. On 70th day, Staphylococcus aureus reduced and lactobacillus increased significantly（P〈0.01）. The intestinal microflora showed an increase in the total aerobic, Clostridium perfringens, enterobacter and enterococcus significantly increased on 20th day（P〈0.05 or 0.01）. Staphylococcus aureus on 50th day increased significantly（P〈0.05）. Total aerobic and enterococcus increased, total anaerobic and Clostridium perfringens reduced significantly on 70th day（P〈0.05 or 0.01）. Conclusions： The microecosystem of respiratory tract and intestine of rat model during the pathological process showed a dynamic disorder, indicating an interaction between the lung and large intestine which may be one of the connotations as they exteriorly and interiorly related.展开更多
OBJECTIVE:To clarify the relationship between helicobacter pylori(Hp)infection,tongue manifestation and gastroscopic manifestation,and explore the mechanism of action of“oral-gastric microecology”in the occurrence a...OBJECTIVE:To clarify the relationship between helicobacter pylori(Hp)infection,tongue manifestation and gastroscopic manifestation,and explore the mechanism of action of“oral-gastric microecology”in the occurrence and development of stomach-related diseases.MEDHODS:A total of 1100 patients were divided into Hp positive group and Hp negative group according to 13 C breath test results.The tongue manifestation materials from patients were collected to make tongue printing slides for morphological observation of exfoliative cells from tongue coating,and electronic gastroscopy was performed on the patients and the data was analyzed.RESULTS:Hp infection rate was about 45.95%;and the greasy coating was mostly observed in Hp positive group(63.14%,P<0.05).The total tongue coating distribution was as follows:yellow greasy coating(56.66%,P<0.05)>yellow coating>white coating>white greasy coating.The gastric mucosal roughness and mucosal hematoplastic plaques in the two groups were more common in the Hp positive group(P<0.05).There was a significant difference in the granular subgroup and in the rough mucosal group(P<0.05).CONCLUSIONS:Hp infection is more commonly observed in yellow greasy coating,and this can be used as a reference for Traditional Chinese Medicine in determining whether it is Hp infection or not.Mucosal granule roughness,hyperemia,and oral Hp showed association with Hp infection in the stomach and interacted with micro-ecological environment that is composed of the oral cavity.展开更多
基金financed by the international cooperation project of Ministry of Science and Technology (2005DFA90200)the mine environment management project of Henan Provincial Department of Land and Resources+1 种基金the basic scientific research project fund of Chinese Academy of Geological Sciences (YYWF201519)China Geology Survey Work Program (121201106000150006)
文摘Experimental study of enhanced in-situ micro-ecological remediation of petroleum contaminated loess soil was carried out in Zhongyuan oil production areas, and the enhanced in-situ micro-ecological remediation technique includes optimistic in-situ microbial communities, physical chemistry methods, alfalfa planting and regulation of soil environmental elements. Experiments showed that the oil content in the contaminated soil with oil content about 2 898.25 mg/kg can be reduced about 98.61% after in-situ micro-ecological remediation for 99 days, which demonstrated the effectiveness of in-situ micro-ecological remediation methods for petroleum contaminated soil in central plains of China, and explored the practical and feasible application of these methods.
文摘Probiotics are a kind of living microorganisms added into food or drug,which are beneficial to our health.Probiotics can regulate the balance of microecosystem in intestine.Recently,people have paid more attention to the effect on the intestinal microecology.This article mainly made a review on the mechanism and application of probiotics.
文摘OBJECTIVE: To observe the effects of Zhenren Yangzang Decoction combined with modified Shenling Baizhu Powder on the immune function and intestinal microecology of diarrhea patients with deficiency and cold syndrome. METHODS: A total of 60 diarrhea patients with deficiency and cold syndrome were randomly divided into observation group and control group, with 30 cases in each group. The control group was treated with conventional western medicine. The observation group was given the treatment of Zhenren Yangzang Decoction combined with modified Shenling Baizhu Powder on the basis of the same treatment for 4 weeks. The clinical symptoms, fecal flora and immunoglobulin content of the 2 groups were observed and the clinical efficacy was evaluated. RESULTS: After treatment, the symptoms scores of fecal diarrhea, aversion to cold, cold limbs, loss of appetite, cold and painful waist and knee, abdominal fullness, abdominal distension and abdominal pain, and intestinal bacilli contents were significantly decreased(P < 0.05). The contents of lactobacillus, bifidus bacilli, IgG, IgM and IgA were significantly increased(P < 0.05), and the improvement of the above indexes in the observation group was significantly better than that in the control group(P < 0.05). The total effective rate after treatment in the observation group was 93.3%, which was significantly higher than 73.3% of the control group(P < 0.05). CONCLUSION: The treatment of diarrhea patients with deficiency and cold syndrome with Zhenren Yangzang Decoction and modified Shenling Baizhu Powder can effectively improve the intestinal micro-ecological environment, improve the immune function of patients and promote the rapid recovery of patients.
文摘The OLIGO+ Project has been launched by PNDC last year further to the observation that the micro-ecology imbalance status in China is responsible for sub-health.Taking the challenge to improve the public health and nutrition,OLIGO+ Project is aiming to raise the issue of micro ecology imbalance to a public level and advocate the addition of Oligo prebiotics in food by food industry.Consumption of prebiotics ingredient will then promote micro-ecological balance and public health.After this first year of successful implementation,OLIGO+ Project will keep the same ambitious objectives for 2009.The soluble fibers,mostly resistant to digestion in the small intestine and largely fermented in the colon demonstrating prebiotic effects have also been included in the OLIGO+ Project.
文摘Mud crab is an important economic crab cultured in Pacific and Indian Ocean Rim. Based on the knowledge about the characteristic requirements in seedling cultivation of mud crab under high-temperature conditions, this article reviews and discusses major factors affecting seedling rearing of mud crabs, and presents novel hatchery facilities for improvement of seedling rearing. The results of our study showed that major factors affecting seedling cultivation of mud crabs included： （1） the micro-ecological environment and characteristics of water quality in seedling rearing ponds, and a double-bottom cultivation facilities and cultivation techniques that meet the requirement; （2） salinity, temperature, light, NOE-N, NH4-N and other physical and chemical environmental factors that affect survival probability ofzoaea; （3） the total number of bacteria in the zoaea-culturing pond and the number of vibrio in seedling rearing; and （4） nutritional intensification with bait organisms in zoaea-culturing. To meet and improve these conditions, a novel hatchery facility was designed to modify the pond roof in accordance with the characteristics of the zoaea stage, and improve natural light thermostat control in seedling cultivation of mud crabs.
文摘It has been proposed that the intestinal microbiota and gastrointestinal tumors are interdependent.Changes in the microbiota can cause dysfunction of the gastrointestinal tract,thereby promoting carcinogenic changes,leading to the occurrence of gastrointestinal tumors.Recent studies on intestinal microbiota have opened up a new area in intestinal micro-ecological immunotherapy.The intestinal microbiota is a double-edged sword.Gut microbes participate in carcinogenesis,but can also be used for immunotherapy.The intestinal microbiota is also regulated by the daily diet.Intestinal micro-ecological immunotherapy combines intestinal immune nutrition and intestinal ecological nutrition to make full use of the intestinal microbiota to strengthen nutritional support.Micro-ecological immunotherapy enhances the body’s immune function by providing energy,improving the functional state of tissues and organs,protecting the intestinal mucosal barrier and maintaining normal intestinal microbiota balance.This involves,to some extent,PD-1 and PD-L1.The microbiota is beneficial to improve the clinical efficacy of conventional anti-cancer therapy and to reduce the incidence of complications.At the same time,micro-ecological immunotherapy is itself active and effective in the perioperative treatment of tumors,which is of great significance for the prognosis of the patient.Gastrointestinal tumors are increasingly linked to intestinal microbiota,and various microbiota-related technologies and drugs have been developed.In the future,the intestinal microbiota may represent a screening marker for gastrointestinal tumors.In addition,clinicians may be able to prevent and treat cancers by changing the gene expression levels of certain microbiota,or by regulating the types of microbes present.
基金support from the National Key Science and Technology Project of China(grant number 2018YFC2000500,03)National Natural Science Foundation of China 81703430 and 81803449,CAMS Innovation Fund for Medical Sciences(grant number 2019-I2M-5-045)the Natural Science Foundation of Zhejiang Province(LYY20H300003).
文摘In-situ oral delivery of therapeutic antibodies,like monoclonal antibody,for chronic inflammation treatment is the most convenient approach compared with other administration routes.Moreover,the abundant links between the gut microbiota and colonic inflammation indicate that the synergistic or antagonistic effect of gut microbiota to colonic inflammation.However,the antibody activity would be significantly affected while transferring through the gastrointestinal tract due to hostile conditions.Moreover,these antibodies have short serum half-lives,thus,require to be frequently administered with high doses to be effective,leading to low patient tolerance.Here,we develop a strategy utilizing thin shell hydrogel microcapsule fabricated by microfluidic technique as the oral delivering carrier.By encapsulating antibodies in these microcapsules,antibodies survive in the hostile gastrointestinal environment and rapidly release into the small intestine through oral administration route,achieving the same therapeutic effect as the intravenous injection evaluated by a colonic inflammation disease model.Moreover,the abundance of some intestinal microorganisms as the indication of the improvement of inflammation has remarkably altered after in-situ antibody-laden microcapsules delivery,implying the restoration of micro-ecology of the intestine.These findings prove our microcapsules are exploited as an efficient oral delivery agent for antibodies with programmable function in clinical application.
基金Supported by the National Basic Research Program of China(973 Program,No.2009CB522706)
文摘Objective： To investigate the mechanism of the Chinese medicine theory that Fei（Lung） and Dachang（Large Intestine） are exteriorly and interiorly related via synchronous observation on the dynamic changes of the respiratory and intestinal microflora. Methods： Forty specific pathogen free Sprague-Dawley rats were selected and randomly divided into blank（10 rats） and chronic bronchitis model groups（30 rats）. The blank group rats were put into the smoke-free environment and the model group rats were put into the smoke environment in order to establish pulmonary disease（chronic bronchitis） model. Then the corresponding changes of the respiratory and intestinal microflora of the model on 20th, 50th and 70th days were synchronously observed. Results： The respiratory tract microflora showed an increase in the total aerobic and Staphylococcus aureus and reduced anaerobic amount significantly on 20th day in the respiratory tract microflora（P〈0.05 or 0.01）. On 50th day, total aerobic, total anaerobic amount and bifidobacterium significantly increased（P〈0.05）. On 70th day, Staphylococcus aureus reduced and lactobacillus increased significantly（P〈0.01）. The intestinal microflora showed an increase in the total aerobic, Clostridium perfringens, enterobacter and enterococcus significantly increased on 20th day（P〈0.05 or 0.01）. Staphylococcus aureus on 50th day increased significantly（P〈0.05）. Total aerobic and enterococcus increased, total anaerobic and Clostridium perfringens reduced significantly on 70th day（P〈0.05 or 0.01）. Conclusions： The microecosystem of respiratory tract and intestine of rat model during the pathological process showed a dynamic disorder, indicating an interaction between the lung and large intestine which may be one of the connotations as they exteriorly and interiorly related.
基金Supported by the Hebei Province Science and Technology Research and Development Project(No.162777113D)the High-level Talent Development Plan of Hebei Province+1 种基金the Special Scientific Research Projects in Traditional Chinese Medicine Industrythe Traditional Chinese Medicine Identification Database(Zang-fu Syndrome Differentiation,Physical Constitution Identification,Health Risk Early Warning)(No.201507003-05)
文摘OBJECTIVE:To clarify the relationship between helicobacter pylori(Hp)infection,tongue manifestation and gastroscopic manifestation,and explore the mechanism of action of“oral-gastric microecology”in the occurrence and development of stomach-related diseases.MEDHODS:A total of 1100 patients were divided into Hp positive group and Hp negative group according to 13 C breath test results.The tongue manifestation materials from patients were collected to make tongue printing slides for morphological observation of exfoliative cells from tongue coating,and electronic gastroscopy was performed on the patients and the data was analyzed.RESULTS:Hp infection rate was about 45.95%;and the greasy coating was mostly observed in Hp positive group(63.14%,P<0.05).The total tongue coating distribution was as follows:yellow greasy coating(56.66%,P<0.05)>yellow coating>white coating>white greasy coating.The gastric mucosal roughness and mucosal hematoplastic plaques in the two groups were more common in the Hp positive group(P<0.05).There was a significant difference in the granular subgroup and in the rough mucosal group(P<0.05).CONCLUSIONS:Hp infection is more commonly observed in yellow greasy coating,and this can be used as a reference for Traditional Chinese Medicine in determining whether it is Hp infection or not.Mucosal granule roughness,hyperemia,and oral Hp showed association with Hp infection in the stomach and interacted with micro-ecological environment that is composed of the oral cavity.
