The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaes：hesia （ or awake） animals. This technique can be used to study the...The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaes：hesia （ or awake） animals. This technique can be used to study the synaptic function and plasticity in central nervous system in vivoin order to understand the physiological properties of the ion channels from an integrated point of view. The advantage of this technique have already presented itself in the study of the synaptic transmission and nervous network. Nowadays, in vivo patch whole-cell recording technique in combination with other techniques is becoming a common method in the research fields.展开更多
The search operation of spatial data was a principal operation in existent spatial database management system, but the update operation of spatial data such as tracking are occurring frequently in the spatial database...The search operation of spatial data was a principal operation in existent spatial database management system, but the update operation of spatial data such as tracking are occurring frequently in the spatial database management system recently. So, necessity of concurrency improvement among transactions is increasing. In general database management system, many techniques have been studied to solve concurrency problem of transaction. Among them, multi version algorithm does to minimize interference among transactions. However, to apply existent multi version algorithm to improve concurrency of transaction to spatial database management system, the waste of storage happens because it must store entire version for spatial record even if only aspatial data of spatial record is changed. This paper has proposed the record management techniques to manage separating aspatial data version and spatial data version to decrease waste of storage for record version and improve concurrency among transactions.展开更多
The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “o...The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.展开更多
文摘The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaes：hesia （ or awake） animals. This technique can be used to study the synaptic function and plasticity in central nervous system in vivoin order to understand the physiological properties of the ion channels from an integrated point of view. The advantage of this technique have already presented itself in the study of the synaptic transmission and nervous network. Nowadays, in vivo patch whole-cell recording technique in combination with other techniques is becoming a common method in the research fields.
基金This work is supported by University IT Research Center ProjectKorea
文摘The search operation of spatial data was a principal operation in existent spatial database management system, but the update operation of spatial data such as tracking are occurring frequently in the spatial database management system recently. So, necessity of concurrency improvement among transactions is increasing. In general database management system, many techniques have been studied to solve concurrency problem of transaction. Among them, multi version algorithm does to minimize interference among transactions. However, to apply existent multi version algorithm to improve concurrency of transaction to spatial database management system, the waste of storage happens because it must store entire version for spatial record even if only aspatial data of spatial record is changed. This paper has proposed the record management techniques to manage separating aspatial data version and spatial data version to decrease waste of storage for record version and improve concurrency among transactions.
文摘The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.
