The characteristics such as signal noise ratio(SNR)[1-2] and sensitivity of the fluorescence detection system for micro-fluidic chip influence the performance of the whole system extremely. The confocal laser induced ...The characteristics such as signal noise ratio(SNR)[1-2] and sensitivity of the fluorescence detection system for micro-fluidic chip influence the performance of the whole system extremely. The confocal laser induced fluorescence detection system is presented. Based on the debugging of optical and circuit modules, the results of detecting the samples are given and analyzed theoretically, and the improved project is put forward.展开更多
In the research of bio-molecular chips and sensors,extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels.In order to accuratel...In the research of bio-molecular chips and sensors,extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels.In order to accurately and flexibly control the bio-molecules as they move within the channels,a clear understanding of how the current changes within the buffer solution caused by an applied bias is fundamental.In this report,the current changed value of different buffer solutions,e.g.,KCl,TE,and TBE was systematically studied with real-time monitoring and quantitative analysis in the situation of the buffers moving through a fluidic channel with a 5 m inner diameter,driven by biases of 50 or 100 mV.The results revealed that the relationship between the current changed value and the pause interval of the applied electric field is highly consistent with the Hill Equation,which is helpful for accurately detecting and manipulating single biomolecules in microfluidic sensors and biochips.展开更多
利用原位聚合法在玻璃微管道内制备阴离子交换型固相萃取(SPE)微柱,以NO-2 为分析对象,针对NaNO2 KI Luminol发光体系设计微流控芯片,并将SPE微柱与微流控芯片连接起来组建成带有SPE微柱的复合式微流控芯片。分析了SPE微柱对NO-2 的吸...利用原位聚合法在玻璃微管道内制备阴离子交换型固相萃取(SPE)微柱,以NO-2 为分析对象,针对NaNO2 KI Luminol发光体系设计微流控芯片,并将SPE微柱与微流控芯片连接起来组建成带有SPE微柱的复合式微流控芯片。分析了SPE微柱对NO-2 的吸附保留与富集作用,在复合式微流控芯片上,实现了NO-2的进样、分离富集和检测,通过漏点曲线和交换容量两种方法分析了SPE微柱的柱容量。为控制SPE微柱的最大进样体积提供有利保障,并实现了食品中NO-2 的在线分离富集与检测。展开更多
基金Key Science and Technology Project Tackled of Guangdong Province(B2050070)
文摘The characteristics such as signal noise ratio(SNR)[1-2] and sensitivity of the fluorescence detection system for micro-fluidic chip influence the performance of the whole system extremely. The confocal laser induced fluorescence detection system is presented. Based on the debugging of optical and circuit modules, the results of detecting the samples are given and analyzed theoretically, and the improved project is put forward.
基金supported by the Major Research Plan of the National Natural Science Foundation of China(Grant No.91123030)the Interna-tional Cooperation Foundation of the National Science and Technology Major Project of the Ministry of Science and Technology of China(Grant No.2011DFA12220)the National Natural Science Foundation of China(Grant No.61378083)
文摘In the research of bio-molecular chips and sensors,extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels.In order to accurately and flexibly control the bio-molecules as they move within the channels,a clear understanding of how the current changes within the buffer solution caused by an applied bias is fundamental.In this report,the current changed value of different buffer solutions,e.g.,KCl,TE,and TBE was systematically studied with real-time monitoring and quantitative analysis in the situation of the buffers moving through a fluidic channel with a 5 m inner diameter,driven by biases of 50 or 100 mV.The results revealed that the relationship between the current changed value and the pause interval of the applied electric field is highly consistent with the Hill Equation,which is helpful for accurately detecting and manipulating single biomolecules in microfluidic sensors and biochips.
