[Objective] The purpose was to select out and identify a flocculant producing strain which could produce high active flocculant.[Method] The strain producing high active flocculant was isolated out and purified throug...[Objective] The purpose was to select out and identify a flocculant producing strain which could produce high active flocculant.[Method] The strain producing high active flocculant was isolated out and purified through medium culture and the selected strain was identified through observing its culture characters and determining its physiological and biochemical property.[Result] Fourteen strains of bacteria with flocculant producing function were isolated from tested soil samples through isolation,purification and preliminary screening using dilution-spread plate method and plate streaking method.Five strains of flocculant producing bacteria showing higher flocculation activity were selected out after second screening and their flocculation rates were higher than 70%;the flocculation activity of one strain among them was still stable after multiple subculturings,its flocculation rate was always above 90% and it was marked as TS-1.TS-1 was encapsulated Gram-positive bacillus and there was no lipid in it,such as poly-β-hydroxybutyric acid.TS-1 was Bacillus amyloliquefaciens,so it was named Bacillus TS-1.[Conclusion] The strain selected out in this experiment could be used in the flocculation and biochemical treatment of wastewater from starch industry.展开更多
This study focuses on the interactions of Hg (II) with microbial biofilms on the basis of a simulated system in which the bacteria isolated from suspended particulate matter in the subtropical——Yangtze River and cla...This study focuses on the interactions of Hg (II) with microbial biofilms on the basis of a simulated system in which the bacteria isolated from suspended particulate matter in the subtropical——Yangtze River and classified as Kluyvera cryocrescens were attached to kaolinite. TOC was the indicator of biomass in the simulated system. The mercury absorbed by bacteria was digested and measured after BrCl oxidation by CVAAS. The kinetic adsorption of resting cell——kaolinite and inactived cell——kaolinite revealed significant differences between the two systems of adsorbents as follows: (1) the mercury adsorption capacity of the resting cell kaolinite was higher than that of the inactived cell kaolinite; (2) the adsorption of resting cell kaolinite increased within 2h and then rapidly decreased from 0.72 μg/g cell(dry weight) to 0.56 μg/g cell. The difference in the affinity to Hg(II) may be attributed to the loss of the metal binding sites of the inactived cell kaolinite during the autoclave treatment, whereas the mercury decline on resting cell kaolinite may be explained by reduction reaction followed by volatilization of Hg(0) because a toxic test on Kluyvera cryocrescens indicated that the bacterial could resist to a high concentration of Hg(II) up to 10.00 mg/L.展开更多
Objective:To investigate the quality of indoor air of different wards and units of Olabisi Onabanjo University Teaching Hospital, Sagamu, to ascertain their contribution to infection rate in the hospital.Methods:The m...Objective:To investigate the quality of indoor air of different wards and units of Olabisi Onabanjo University Teaching Hospital, Sagamu, to ascertain their contribution to infection rate in the hospital.Methods:The microbial quality of indoor air of nine wards/units of Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria was conducted. Sedimentation technique using open Petri-dishes containing different culture media was employed and samplings were done twice daily, one in the morning shortly after cleaning and before influx of people/patients into the wards/units and the other in the evening when a lot of activities would have taken place in these wards. Isolates were identified according to standard methods.Results:Results showed that there was a statistically significant difference(氈2= 6.0167) in the bacteria population of the different sampling time whereas it was not so for fungi population(氈2= 0.2857). Male medical ward(MMW) and male surgical general(MSG) recorded the highest bacterial and fungal growth while the operating theatre(OT) was almost free of microbial burden. The bacteria isolates were Staphylococcus aureus, Klebsiellasp., Bacillus cereus, Bacillus subtilis, Streptococcus pyogenes andSerratia marscenceswhile the fungi isolates includedAspergillus flavus, Penicilliumsp.,Fusariumsp.,Candida albicansandAlternariasp.Staphylococcus aureuswas the predominantly isolated bacterium whilePenicilliumsp. was the most isolated fungus.Conclusions:Though most of the microbial isolates were potential and or opportunistic pathogens, there was no correlation between the isolates in this study and the surveillance report of nosocomial infection during the period of study, hence the contribution of the indoor air cannot be established. From the reduction noticed in the morning samples, stringent measures such as proper disinfection and regular cleaning, restriction of patient relatives' movement in and out of the wards/units need to be enforced so as to improve the quality of indoor air of our hospital wards/units.展开更多
Aerobic granule is a special microbial aggregate associated with biofilm structure.The formation of aerobic granular sludge is primarily depending on its bacterial community and relevant microbiological properties.In ...Aerobic granule is a special microbial aggregate associated with biofilm structure.The formation of aerobic granular sludge is primarily depending on its bacterial community and relevant microbiological properties.In this experiment,a strain with high microbial attachment was isolated from aerobic granular sludge,and the detailed characteristics were examined.Its high attachment ability could reach 2.34(OD600 nm),while other low attachment values were only around 0.06-0.32,which indicated a big variation among the different bacteria.The strain exhibited a very special morphology with many fibric fingers under SEM observation.A distinctive behaviour was to form a spherical particle by themselves,which would be very beneficial for the formation and development of granular sludge.The EPS measurement showed that its PN content was higher than low attachment bacteria,and 3 DEEM confirmed that there were some different components.Based on the 16 S rRNA analysis,it was identified to mostly belong to Stenotrophomonas.Its augmentation to particle sludge cultivation demonstrated that the strain could significantly promote the formation of aerobic granule.Conclusively,it was strongly suggested that it might be used as a good and potential model strain or chassis organism for the aerobic granular sludge formation and development.展开更多
bacterium LV-1 which isolated from soil sample were studied bacterium was isolated by serial dilution method, the effects of carbon source, nitrogen source, the initial pH and temperature on producing polysaccharide b...bacterium LV-1 which isolated from soil sample were studied bacterium was isolated by serial dilution method, the effects of carbon source, nitrogen source, the initial pH and temperature on producing polysaccharide by it were discussed to confirm the optimum fermentation conditions. [ Result] The physicochemical properties showed that the polysaccharide was water-soluble, but insoluble in organic solvents including ethanol, butanol, and chloroform. It was neutral polysaccharide with negative charge and without reducing terminal. The pH of its solution was pH =7.5. There were no protein, fructose, uronic acid, sulphate and starch-like structure included in po/ysacchadde molecules. The optimum fermentation conditions for po/ysaccharide produc- tion were 3% mannitol as carbon source, 0.25% yeast extract as nitrogen source, culture temperature 28 ~（3 and pH =7.5. [Coadu^en] The re- search could provide basis for development and utilization of LV-1 and industrialized production of mucopolysaccharide.展开更多
基金Supproted by the Key Project of Chinese Ministry of Education(211189)~~
文摘[Objective] The purpose was to select out and identify a flocculant producing strain which could produce high active flocculant.[Method] The strain producing high active flocculant was isolated out and purified through medium culture and the selected strain was identified through observing its culture characters and determining its physiological and biochemical property.[Result] Fourteen strains of bacteria with flocculant producing function were isolated from tested soil samples through isolation,purification and preliminary screening using dilution-spread plate method and plate streaking method.Five strains of flocculant producing bacteria showing higher flocculation activity were selected out after second screening and their flocculation rates were higher than 70%;the flocculation activity of one strain among them was still stable after multiple subculturings,its flocculation rate was always above 90% and it was marked as TS-1.TS-1 was encapsulated Gram-positive bacillus and there was no lipid in it,such as poly-β-hydroxybutyric acid.TS-1 was Bacillus amyloliquefaciens,so it was named Bacillus TS-1.[Conclusion] The strain selected out in this experiment could be used in the flocculation and biochemical treatment of wastewater from starch industry.
文摘This study focuses on the interactions of Hg (II) with microbial biofilms on the basis of a simulated system in which the bacteria isolated from suspended particulate matter in the subtropical——Yangtze River and classified as Kluyvera cryocrescens were attached to kaolinite. TOC was the indicator of biomass in the simulated system. The mercury absorbed by bacteria was digested and measured after BrCl oxidation by CVAAS. The kinetic adsorption of resting cell——kaolinite and inactived cell——kaolinite revealed significant differences between the two systems of adsorbents as follows: (1) the mercury adsorption capacity of the resting cell kaolinite was higher than that of the inactived cell kaolinite; (2) the adsorption of resting cell kaolinite increased within 2h and then rapidly decreased from 0.72 μg/g cell(dry weight) to 0.56 μg/g cell. The difference in the affinity to Hg(II) may be attributed to the loss of the metal binding sites of the inactived cell kaolinite during the autoclave treatment, whereas the mercury decline on resting cell kaolinite may be explained by reduction reaction followed by volatilization of Hg(0) because a toxic test on Kluyvera cryocrescens indicated that the bacterial could resist to a high concentration of Hg(II) up to 10.00 mg/L.
文摘Objective:To investigate the quality of indoor air of different wards and units of Olabisi Onabanjo University Teaching Hospital, Sagamu, to ascertain their contribution to infection rate in the hospital.Methods:The microbial quality of indoor air of nine wards/units of Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria was conducted. Sedimentation technique using open Petri-dishes containing different culture media was employed and samplings were done twice daily, one in the morning shortly after cleaning and before influx of people/patients into the wards/units and the other in the evening when a lot of activities would have taken place in these wards. Isolates were identified according to standard methods.Results:Results showed that there was a statistically significant difference(氈2= 6.0167) in the bacteria population of the different sampling time whereas it was not so for fungi population(氈2= 0.2857). Male medical ward(MMW) and male surgical general(MSG) recorded the highest bacterial and fungal growth while the operating theatre(OT) was almost free of microbial burden. The bacteria isolates were Staphylococcus aureus, Klebsiellasp., Bacillus cereus, Bacillus subtilis, Streptococcus pyogenes andSerratia marscenceswhile the fungi isolates includedAspergillus flavus, Penicilliumsp.,Fusariumsp.,Candida albicansandAlternariasp.Staphylococcus aureuswas the predominantly isolated bacterium whilePenicilliumsp. was the most isolated fungus.Conclusions:Though most of the microbial isolates were potential and or opportunistic pathogens, there was no correlation between the isolates in this study and the surveillance report of nosocomial infection during the period of study, hence the contribution of the indoor air cannot be established. From the reduction noticed in the morning samples, stringent measures such as proper disinfection and regular cleaning, restriction of patient relatives' movement in and out of the wards/units need to be enforced so as to improve the quality of indoor air of our hospital wards/units.
基金supported by the National Natural Science Foundation of China(No.51578069)Beijing Science and Technology Commission Project(No.Z171100000717012)。
文摘Aerobic granule is a special microbial aggregate associated with biofilm structure.The formation of aerobic granular sludge is primarily depending on its bacterial community and relevant microbiological properties.In this experiment,a strain with high microbial attachment was isolated from aerobic granular sludge,and the detailed characteristics were examined.Its high attachment ability could reach 2.34(OD600 nm),while other low attachment values were only around 0.06-0.32,which indicated a big variation among the different bacteria.The strain exhibited a very special morphology with many fibric fingers under SEM observation.A distinctive behaviour was to form a spherical particle by themselves,which would be very beneficial for the formation and development of granular sludge.The EPS measurement showed that its PN content was higher than low attachment bacteria,and 3 DEEM confirmed that there were some different components.Based on the 16 S rRNA analysis,it was identified to mostly belong to Stenotrophomonas.Its augmentation to particle sludge cultivation demonstrated that the strain could significantly promote the formation of aerobic granule.Conclusively,it was strongly suggested that it might be used as a good and potential model strain or chassis organism for the aerobic granular sludge formation and development.
文摘bacterium LV-1 which isolated from soil sample were studied bacterium was isolated by serial dilution method, the effects of carbon source, nitrogen source, the initial pH and temperature on producing polysaccharide by it were discussed to confirm the optimum fermentation conditions. [ Result] The physicochemical properties showed that the polysaccharide was water-soluble, but insoluble in organic solvents including ethanol, butanol, and chloroform. It was neutral polysaccharide with negative charge and without reducing terminal. The pH of its solution was pH =7.5. There were no protein, fructose, uronic acid, sulphate and starch-like structure included in po/ysacchadde molecules. The optimum fermentation conditions for po/ysaccharide produc- tion were 3% mannitol as carbon source, 0.25% yeast extract as nitrogen source, culture temperature 28 ~（3 and pH =7.5. [Coadu^en] The re- search could provide basis for development and utilization of LV-1 and industrialized production of mucopolysaccharide.
