Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological en...Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological endpoints of Microcystis aeruginosa,including the composition and functional groups of soluble EPS(SL-EPS),loosely bound EPS(LB-EPS),and tightly bound EPS(TB-EPS),were detected to elucidate the toxicity and detoxification mechanisms of Cd(Ⅱ)for cyanobacteria.Toxicological and physiological assays on M.aeruginosa showed that the 0.25-mg/L Cd(Ⅱ)resulted in a larger inhibition on growth and F_(v)/F_(m).Nevertheless,Cd(Ⅱ)significantly induced much higher contents of superoxide dismutase(SOD),intracellular microcystin LR(MC-LR),extracellular MC-LR,and EPS.Scanning electron microscopy with energy dispersive X-ray spectroscopy confirmed that Cd(Ⅱ)was absorbed into the EPS layer.Fourier transform infrared spectrum analysis revealed that the functional groups bound with Cd(Ⅱ)of algae biomass,SL-EPS,LB-EPS,and TB-EPS were somewhat different.The C=O/C=N groups ofδ-lactam or protein were their prominent functional groups,suggesting that amide or proteins in the EPS played a key role in the adsorption in Cd(Ⅱ).The concentration of 0.25 mg/L of Cd(Ⅱ)may change the chemical structure of EPS by altering the production of protein-like substances containing tryptophan.This study indicated that M.aeruginosa could detoxify Cd(Ⅱ)stress via induction of antioxidant capacity(higher SOD activity and MC synthesis),EPS production,and modification in chemical structure of EPS.展开更多
[Objective] The study aimed to discuss the effects of pH value on the growth metabolism of Microcystis aeruginosa and the phosphorus metabolism relationship with adnascent Pseudomonas.[Method] By the phosphorus uptake...[Objective] The study aimed to discuss the effects of pH value on the growth metabolism of Microcystis aeruginosa and the phosphorus metabolism relationship with adnascent Pseudomonas.[Method] By the phosphorus uptake experiment of M.aeruginosa under different pH conditions(8.0-10.0) and the effect experiment on the phosphorus metabolism of M.aeruginosa and adnascent Pseudomonas under different pH conditions(7.0-9.0),the phosphorus uptake of M.aeruginosa in the short time and the growth curve of M.aeruginosa,the change of phosphorus concentration in the water,the change of total phosphorus content in M.aeruginosa in the longer time were measured.[Results] In the short time,pH value had the effects on the absorption phosphorus ability of M.aeruginosa.As pH value rose,the absorption phosphorus ability enhanced.During the longer time,the higher pH value was,the quicker the growth speed of M.aeruginosa was,and the better the growth situation was.M.aeruginosa had the ability of self regulation pH value and could use the phosphorus well in the water which was released from Pseudomonas.In the system of the algae,bacteria and water,the phosphorus in the bacteria played the role of phosphorus source which was released slowly.Though the phosphorus concentration was lower,it was favorable to the growth of algae.[Conclusions] pH value was the factor that affected the circle of the phosphorus element in the system of algae-bacteria-water.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31800457,32170383)。
文摘Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological endpoints of Microcystis aeruginosa,including the composition and functional groups of soluble EPS(SL-EPS),loosely bound EPS(LB-EPS),and tightly bound EPS(TB-EPS),were detected to elucidate the toxicity and detoxification mechanisms of Cd(Ⅱ)for cyanobacteria.Toxicological and physiological assays on M.aeruginosa showed that the 0.25-mg/L Cd(Ⅱ)resulted in a larger inhibition on growth and F_(v)/F_(m).Nevertheless,Cd(Ⅱ)significantly induced much higher contents of superoxide dismutase(SOD),intracellular microcystin LR(MC-LR),extracellular MC-LR,and EPS.Scanning electron microscopy with energy dispersive X-ray spectroscopy confirmed that Cd(Ⅱ)was absorbed into the EPS layer.Fourier transform infrared spectrum analysis revealed that the functional groups bound with Cd(Ⅱ)of algae biomass,SL-EPS,LB-EPS,and TB-EPS were somewhat different.The C=O/C=N groups ofδ-lactam or protein were their prominent functional groups,suggesting that amide or proteins in the EPS played a key role in the adsorption in Cd(Ⅱ).The concentration of 0.25 mg/L of Cd(Ⅱ)may change the chemical structure of EPS by altering the production of protein-like substances containing tryptophan.This study indicated that M.aeruginosa could detoxify Cd(Ⅱ)stress via induction of antioxidant capacity(higher SOD activity and MC synthesis),EPS production,and modification in chemical structure of EPS.
基金Supported by"973"Project(2008CB418003)The National Natural Science Fund(30700414)
文摘[Objective] The study aimed to discuss the effects of pH value on the growth metabolism of Microcystis aeruginosa and the phosphorus metabolism relationship with adnascent Pseudomonas.[Method] By the phosphorus uptake experiment of M.aeruginosa under different pH conditions(8.0-10.0) and the effect experiment on the phosphorus metabolism of M.aeruginosa and adnascent Pseudomonas under different pH conditions(7.0-9.0),the phosphorus uptake of M.aeruginosa in the short time and the growth curve of M.aeruginosa,the change of phosphorus concentration in the water,the change of total phosphorus content in M.aeruginosa in the longer time were measured.[Results] In the short time,pH value had the effects on the absorption phosphorus ability of M.aeruginosa.As pH value rose,the absorption phosphorus ability enhanced.During the longer time,the higher pH value was,the quicker the growth speed of M.aeruginosa was,and the better the growth situation was.M.aeruginosa had the ability of self regulation pH value and could use the phosphorus well in the water which was released from Pseudomonas.In the system of the algae,bacteria and water,the phosphorus in the bacteria played the role of phosphorus source which was released slowly.Though the phosphorus concentration was lower,it was favorable to the growth of algae.[Conclusions] pH value was the factor that affected the circle of the phosphorus element in the system of algae-bacteria-water.
