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Analysis of promoter activity reveals that GmFTL2 expression differs from that of the known Flowering Locus T genes in soybean 被引量:2
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作者 Limin Liu Xiaomei Zhang +4 位作者 Fulu Chen Asia Adam Elzamzami Mahi Xiaoxia Wu Qingshan Chen Yong-Fu Fu 《The Crop Journal》 SCIE CAS CSCD 2017年第5期438-447,共10页
Regulation of flowering is one of the key issues in crop yield. The Floiuering Loews T(FT) gene is a well-known florigen, which integrates various signals from multiple flowering-regulation pathways to initiate flower... Regulation of flowering is one of the key issues in crop yield. The Floiuering Loews T(FT) gene is a well-known florigen, which integrates various signals from multiple flowering-regulation pathways to initiate flowering. We previously reported that there are at least six FT genes(GmFTLl-6) in soybean displaying flowering activity. However, the individual functions of genes GmFTLl-6 remain to be identified. In this study, we cloned the GmFTL2 promoter(GmFTLpro) from soybean(Glycine max) cultivar Tianlong 1 and analyzed its motifs bioinformatically and its expression patterns using both a transgenic approach and quantitative RT-PCR(qRT-PCR). In GmFTLpro::GUS transgenic lines, GUS signals were enriched in cotyledons, hypocotyledons, pollen, embryos, and root tips in a photoperiod-independent manner. qRT-PCR confirmed the GUS reporter results. Our results suggest that GmFTL2 expression is regulated by developmental and tissue-specific clues and plays roles in seedling establishment and the development of micro game tophytes, embryos, and roots. 展开更多
关键词 FLOWERING LOCUS T COTYLEDON microgametophyte EMBRYO Root tip
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