Correlation of serum free fatty acid content with glucolipid metabolism, microinflammation and oxidative stress state in patients with type 2 diabetes mellitus

Objective: To investigate the correlation of serum free fatty acid content with glucolipid metabolism, microinflammation and oxidative stress state in patients with type 2 diabetes mellitus. Methods: A total of 189 patients with type 2 diabetes mellitus who were treated in this hospital between August 2015 and February 2018 were chosen as type 2 diabetes mellitus group, and 100 healthy subjects who received physical examination in this hospital during the same period were chosen as the normal control group. The differences in serum levels of FFA, glucolipid metabolism indexes, microinflammation indexes and oxidative stress indexes were compared between the two groups of subjects, and correlation analysis was adopted to judge the inner link of serum FFA content with glucolipid metabolism, microinflammation and oxidative stress state in patients with type 2 diabetes mellitus. Results: Serum FFA content of type 2 diabetes mellitus group was higher than that of control group;serum glucolipid metabolism indexes FPG, 2hPG, TC and LDL-C levels were higher than those of normal control group whereas HDL-C level was lower than that of control group;serum microinflammation indexes IL-1β, IL-6, IL-10, IL-15 and TNF-α contents were higher than those of control group;serum oxidative stress indexes GSH-Px and SOD contents were lower than those of control group whereas ROS and MDA contents were higher than those of control group. Pearson test showed that the serum FFA content of patients with type 2 diabetes mellitus was directly correlated with the contents of glucolipid metabolism, microinflammation and oxidative stress indexes. Conclusion: Serum FFA content significantly increases in patients with type 2 diabetes mellitus, and the specific content was directly correlated with glycolipid metabolism, microinflammation, oxidative stress and other illness indexes.

Correlation of anxiety state with blood glucose control, microinflammation and oxidative stress in patients with type 2 diabetes mellitus

Objective:To study the correlation of anxiety state with blood glucose control, microinflammation and oxidative stress in patients with type 2 diabetes mellitus.Methods:A total of 138 patients with type 2 diabetes mellitus were divided into the non-anxiety group (n=43) (SAS score < 50 points), mild-to-moderate anxiety group (n=71) (SAS score 50-69 points) and severe anxiety group (n=24) (SAS score 70 points) according to the self-rating anxiety scale (SAS) score. The differences in levels of glucose metabolism indexes, microinflammation indexes and oxidative stress indexes were compared among the three groups.Results: Peripheral blood FPG, P2hPG, HOMA-IR, CRP, ASAA, IL-6, MDA and ROS levels of mild-to-moderate anxiety group and severe anxiety group were higher than those of non-anxiety group while CAT and GSH-Px contents were lower than those of non-anxiety group;peripheral blood FPG, P2hPG, HOMA-IR, CRP, ASAA, IL-6, MDA and ROS levels of severe anxiety group were higher than those of mid-to-moderate anxiety group while CAT and GSH-Px contents were lower than those of mid-to-moderate anxiety group.Conclusion:The increase of anxiety in patients with type 2 diabetes mellitus is a direct factor leading to the abnormal glucose metabolism as well as the aggravation of microinflammatory state and oxidative stress state.

Microinflammation is involved in the dysfunction of arteriovenous fistula in patients with maintenance hemodialysis

Background Vascular access （VA） dysfunction is a major clinical complication in the hemodialysis population and has a direct effect on dialysis outcome. This study was conducted to explore the role of microinflammation in the VA dysfunction in maintenance hemodialysis patients. Methods Forty-seven patients （male 35 and female 12） receiving maintenance hemodialysis were included for this study. They were divided into three groups： group 1 （n=15）, patients with initial hemodialysis and new arteriovenous fistula （AVF）; group 2 （n=18）, patients treated with hemodialysis for long term with well-functional VA; group 3 （n=14）, maintenance hemodialysis patients with VA dysfunction. Biochemical parameters and serum tumor necrosis factor-α （TNF-α）, interleukin 6 （IL-6） and monocyte chemoattractant protein-1 （MCP-1） were determined. High-sensitivity C-reactive protein （hs-CRP） was determined by latex-enhanced immuno-nephelometric method. Tissues of radial artery were taken from group 1 and group 3 for the histological study. Expression of CD68 and MCP-1 in the radial artery was determined by immunohistochemistry. Results Serum hs-CRP in group 3 was significantly higher than those in group 1 and group 2 （（7.40±2.42） mg/L vs （4.21±1.62） mg/L and （5.04±3.65） mg/L, P 〈0.01 and P 〈0.05, respectively）. Serum TNF-α in group 3 was significantly higher than those in group 1 and group 2 （（64.03±9.29） pg/ml vs （54.69±12.39） pg/ml and （54.05±7.68） pg/ml, P 〈0.05 and P 〈0.01, respectively）. Serum IL-6 in group 3 was also significantly higher than those in group 1 and group 2 （（70.09±14.53） pg/ml vs （56.43±10.11） pg/ml and （60.77±9.70） pg/ml, P 〈0.01 and P 〈0.05, respectively）. Patients in group 3 had a thicker internal layer of vessels than in group 1 （（0.356±0.056） mm vs （0.111±0.021） mm, P 〈0.01）. Expression of CD68 and MCP-1 in the fistula vessel walls in group 3 were much higher than those in group 1 （P 〈0.01）. Moreover, serum hs-CRP level was positively correlated with the neointimal hyperplasia, the expression of CD68 and MCP-1 in fistula vessel （P 〈0.01, respectively）. Conclusion Microinflammaton might be involved in the dysfunction of AVF in patients with maintenance hemodialysis.

Pilomatrixoma causing irreversible peripheral eyebrow shedding:A case report

Pilomatrixomas are benign tumors derived from hair stromal cells;however,their exact etiology is unknown.A 31-year-old woman presented with a subcutaneous mass located precisely above the left eyebrow,with shedding of eyebrow hair around the mass,and the shedding area gradually expanded.Surgical removal of the mass failed to prevent eyebrow loss,and the entire left eyebrow shed 6 months postoperatively.Pathology and histology of the biopsy specimen revealed a pilomatrixoma comprising basaloid cells and ghost cells,with basaloid cells expressing high bcl-2 levels.Instead of apoptosis,the emergence and growth of pilomatrixoma-induced microinflammation around the hair follicles of the eyebrow may lead to eyebrow loss.