Micromelalopha troglodyta （Graeser） is an important pest of poplar in China. Glutathione S-transferases （GSTs） are known to be responsible for adaptation mechanisms of M. troglodyta. The activities and kinetic con...Micromelalopha troglodyta （Graeser） is an important pest of poplar in China. Glutathione S-transferases （GSTs） are known to be responsible for adaptation mechanisms of M. troglodyta. The activities and kinetic constants of glutathione S-transferases in M. troglodyta were studied. Significant differences in glutathione S-transferase activity and kinetic characteristics were observed among five instars of M. troglodyta larvae. Furthermore, the inhibition of glutathione S-transferase activity in five instars by 24 inhibitors was conducted. The results show the inhibition of GST activity of different instars by 24 inhibitors was different. For GST activity in the 1st instar, chlorpyrifos, lambda-cyhalothrin, endosulfan, abamectin, fipronil and pyridaben were the best inhibitors tested, and for GST activity in the 2nd instar, tannic acid and quercetin were the most potent inhibitors tested, and for GST activity in the 3rd instar, the inhibitory effects of quercetin, chlorpyrifos and lambda-cyhalothrin were the highest, and for GST activity in the 4th instar, quercetin and lambda-cyhalothrin were the best inhibitors, and the inhibitory effect of phoxim was the highest for GST activity in the 5th instar. Our results show that glutathione S-transferases in different instars are qualitatively different in isozyme composition and thus different in sensitivity to inhibitors.展开更多
利用飞机喷洒25%阿维·灭幼脲3号悬浮剂,对添加不同比例高效氯氰菊酯和不同施药时期的防治效果进行研究,试验发现每架次添加1.5 kg 5%高效氯氰菊酯油剂(两种药剂的质量比为24∶1.5),能快速有效防治杨树舟蛾类害虫。6月中下旬豫南地...利用飞机喷洒25%阿维·灭幼脲3号悬浮剂,对添加不同比例高效氯氰菊酯和不同施药时期的防治效果进行研究,试验发现每架次添加1.5 kg 5%高效氯氰菊酯油剂(两种药剂的质量比为24∶1.5),能快速有效防治杨树舟蛾类害虫。6月中下旬豫南地区杨树舟蛾类害虫为二代2～3龄幼虫期,是施药防治该类害虫的最佳时期。展开更多
基金supported by National Natural Science Foundation of China(Contract No.30600476 and 30972376)
文摘Micromelalopha troglodyta （Graeser） is an important pest of poplar in China. Glutathione S-transferases （GSTs） are known to be responsible for adaptation mechanisms of M. troglodyta. The activities and kinetic constants of glutathione S-transferases in M. troglodyta were studied. Significant differences in glutathione S-transferase activity and kinetic characteristics were observed among five instars of M. troglodyta larvae. Furthermore, the inhibition of glutathione S-transferase activity in five instars by 24 inhibitors was conducted. The results show the inhibition of GST activity of different instars by 24 inhibitors was different. For GST activity in the 1st instar, chlorpyrifos, lambda-cyhalothrin, endosulfan, abamectin, fipronil and pyridaben were the best inhibitors tested, and for GST activity in the 2nd instar, tannic acid and quercetin were the most potent inhibitors tested, and for GST activity in the 3rd instar, the inhibitory effects of quercetin, chlorpyrifos and lambda-cyhalothrin were the highest, and for GST activity in the 4th instar, quercetin and lambda-cyhalothrin were the best inhibitors, and the inhibitory effect of phoxim was the highest for GST activity in the 5th instar. Our results show that glutathione S-transferases in different instars are qualitatively different in isozyme composition and thus different in sensitivity to inhibitors.
文摘利用飞机喷洒25%阿维·灭幼脲3号悬浮剂,对添加不同比例高效氯氰菊酯和不同施药时期的防治效果进行研究,试验发现每架次添加1.5 kg 5%高效氯氰菊酯油剂(两种药剂的质量比为24∶1.5),能快速有效防治杨树舟蛾类害虫。6月中下旬豫南地区杨树舟蛾类害虫为二代2～3龄幼虫期,是施药防治该类害虫的最佳时期。
