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The Chromosomal Effect of Birchen Dust as Determined by the Micronucleus Test
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作者 JIANG ZHI-CHENG SU YI-LAN +5 位作者 DENG YI-FU SUN WEI LIU WEI-CHENG WEI DAI-MING YAN JIU-YEN YAO YU-LONGn(Deprtment of Occupotional Health, Chpital University ofMedical Sciences, Beijing, 100054, China)(Sanitationand Anti-ePidemic Station, tongiingcheng Fore 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1997年第4期396-401,共6页
In a wood processing factory, the measured air concentration of birchen dust was 1. 26 ±0. 41 mg/m3, and the micronucleus frequency of peripheral blood lymphocytes in 83 workersexposed to wood dust was 1. 13 ... In a wood processing factory, the measured air concentration of birchen dust was 1. 26 ±0. 41 mg/m3, and the micronucleus frequency of peripheral blood lymphocytes in 83 workersexposed to wood dust was 1. 13 ± 2. 83%, which was significantly higher (P < 0.01 ) thanthat of control group (0. 51 ± 1. 41% ). The number of exposed workers with positive mi-cronucleus test was 9. 6 %, which was higher than that of control group (4. 5 % ), but thedifference was not significant (P >0. 05 ). The micronucleus test in mice treated with waterextracts of unsteamed and unbaked birchen dust showed that the micronucleus frequencies inall treated groups were significantly higher than that of contro group (P < 0. 01 ) and therewas also a doseresponse correlation (r = 0. 96, P < 0. 0005 ). The results of steamed andbaked birchen dust extracts were significantly lower than those of the unsteamed and unbakedones at the same doses (P< 0. 001 ). This suggests that when the birchen dust is steamed atthe temperature of 100℃ for 24h or baked at the temperature of 80℃, its inducing effect inmicronucleus test could be lowered 展开更多
关键词 The Chromosomal Effect of Birchen Dust as Determined by the micronucleus test CHEN
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Comet assay and cytokinesis-blocked micronucleus test for monitoring the genotoxic effects of X-ray radiation in humans 被引量:4
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作者 何继亮 陈玮琳 +1 位作者 金力奋 金海燕 《Chinese Medical Journal》 SCIE CAS CSCD 2000年第10期47-50,共4页
Obejctive To assess the genotoxic effects of X ray radiation on human populations Methods The single cell gel electrophoresis (SCGE) and cytokinesis blocked micronucleus (CBMN) test were applied as biological dos... Obejctive To assess the genotoxic effects of X ray radiation on human populations Methods The single cell gel electrophoresis (SCGE) and cytokinesis blocked micronucleus (CBMN) test were applied as biological dosimeters to detect DNA damage and abnormalities in human peripheral lymphocytes of subpopulation exposed to X ray radiation The subjects were divided into four groups: 12 radiation patients; 13 intervention radiation therapy doctors; 32 radiation diagnostians; 28 controls Results The average comet lengths of the four groups were 128 17±4 49?μm, 88 09±5 39?μm, 72 68±2 57?μm and 32 87±0 57?μm, respectively The difference in average comet length between any two groups was highly significant ( P <0 01) The average micronucleated cell (MNC) rates (‰) of the four groups were 12 33±0 85, 9 75±1 02, 8 48±0 66 and 3 18±0 36, respectively The difference of MNC rates of Group 1 vs 3, 1 vs 4, 2 vs 4 and 3 vs 4 was highly significant ( P <0 01), and the difference of Group 1 vs 2 was significant ( P <0 05), but there was no difference of MNC rate in Group 2 vs 3 ( P >0 05) Conclusions This study showed that both the comet assay and the CBMN test could be used to monitor populations exposed to X ray radiation, but the comet assay seems to be more sensitive than the CBMN test 展开更多
关键词 X-ray radiation DNA damage single cell gel electrophoresis assay cytokinesis-blocked micronucleus test comet assay
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Screening potential genotoxic effect of aquatic plant extracts using the mussel micronucleus test
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作者 Bettina Eck-Varanka Nora Kováts +3 位作者 Katalin Hubai Gábor Paulovits Árpád Ferincz Eszter Horváth 《Journal of Coastal Life Medicine》 2016年第1期36-38,共3页
Objective:To assess the genotoxic potential of selected aquatic macrophytes:Ceratophyllum demersum L.(hornwort,family Ceratophyllaceae),Typha angustifolia L.(narrowleaf cattail,family Typhaceae),Stratiotes aloides L.(... Objective:To assess the genotoxic potential of selected aquatic macrophytes:Ceratophyllum demersum L.(hornwort,family Ceratophyllaceae),Typha angustifolia L.(narrowleaf cattail,family Typhaceae),Stratiotes aloides L.(water soldier,family Butomaceae),and Oenanthe aquatica(L.)Poir.(water dropwort,family Umbelliferae).Methods:For genotoxicity assessment,the mussel micronucleus test was applied.Micronucleus frequency was determined from the haemolymph of Unio pictorum L.(painter’s mussel).In parallel,total and hydrolisable tannin contents were determined.Results:All plant extracts elucidated significant mutagenic effect.Significant correlation was determined between tannin content and mutagenic capacity.Conclusions:The significant correlation between genotoxicity as expressed by micronucleus frequency and tannin content(both total and hydrolisable tannins)indicate that tannin is amongst the main compounds being responsible for the genotoxic potential.It might be suggested that genotoxic capacity of these plants elucidate a real ecological effect in the ecosystem. 展开更多
关键词 GENOTOXICITY Mussel micronucleus test Unio pictorum TANNIN
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The Preliminary Study of Certain Pharmacological Effects of Xi Xin Oil 被引量:2
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作者 胡月娟 徐军 纪绿屏 《Journal of Chinese Pharmaceutical Sciences》 CAS 1993年第2期156-158,共3页
细辛油能松弛兔离体主动脉条,使去甲肾上腺素所致主动脉收缩的量效曲线右移并效能降低;使离体兔耳灌流量减少;能延长小鼠常压耐缺氧时间;用其1/2 LD_(50)之剂量腹腔注射给药3天,可致小鼠骨髓微核形成增加。
关键词 Xi Xin Oil Aorta strip Anoxia tolerance micronucleus test
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Drinking water and liver cancer *
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作者 阮萃才 陈艳华 张振权 《World Journal of Gastroenterology》 SCIE CAS CSCD 1997年第1期49-52,共4页
AIM To study the relationship between mutagenicity of drinking water and incidence of liver cancer in high liver cancer incidence areas in Guangxi.
关键词 Drinking water Liver neoplasms legumes micronucleus tests
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Study on the water quality of the Taihu Lake using genotoxicological methods
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作者 Wu Qing long, Chen Kai ning Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China Kong Zhi ming, Zang Yu The State Key Laboratory of Pollution Control and Resource Reuse, Department of Environmental S 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 1998年第1期105-111,共7页
The micronucleus(MCN) test of Vicia faba root tip cell was used to screen the water quality of the Taihu Lake. The MCN ‰ and the pollution index ( PI ) were examined and the F test was carried out to ev... The micronucleus(MCN) test of Vicia faba root tip cell was used to screen the water quality of the Taihu Lake. The MCN ‰ and the pollution index ( PI ) were examined and the F test was carried out to evaluate the statistical difference in mean MCN ‰ among various samples. There were significant difference among thirty nine samples and there were six sites whose PI values are above 2. The authors suggest that the Taihu Lake has partly been polluted to different degrees and the whole water body can be divided into three types. Ames tests were then conducted to detect mutagenicity at five significant sites. The results showed that rivers flowing through urban area carried large amount of mutagenic pollutants into the lake and these pollutants contaminated the source of main waterworks. 展开更多
关键词 Taihu Lake water quality micronucleus test Ames test.
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Genotoxic Effects in Mice Exposed to Radon Emissions in Indoor Conditions. Comparison between in utero and Neonatal Exposures
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作者 Cristiano Foschi Manuel Luis Orta +2 位作者 Licia Radicchi Germana Szpunar Mauro Cristaldi 《Journal of Life Sciences》 2016年第2期66-76,共11页
The purpose of this study was to develop a biological model to evaluate the genotoxic effects of natural emissions of Radon-222 and its decay products. To this aim, mice of the Swiss CD1 strain were exposed to Radon f... The purpose of this study was to develop a biological model to evaluate the genotoxic effects of natural emissions of Radon-222 and its decay products. To this aim, mice of the Swiss CD1 strain were exposed to Radon for different periods (adult life, early postnatal and in utero exposure) and two simple but accurate mutagenicity tests (Micronucleus test and the Comet assay) were applied to the peripheral blood of mice. The study was carried out in two small towns in Latium region--ltaly, where radon pollution is notoriously present due to the volcanic soils. One experiment was carried out in the cellar of a house in Ciampino (Rome) and two experiments were performed in an old cellar in Vetralla (Viterbo). The results showed that in all mice groups exposed to natural emissions of radon and its decay products, the micronucleated erythrocytes frequency (ME) was significantly higher than that observed in the mice control. The single cell gel electrophoresis (Comet assay) was performed in lymphocytes of adult mice in the last experiment. The results for this test also show that DNA damage was higher than in the cells of the mice control and the cells of mice exposed for a shorter period of time. To confirm these findings, the single cell gel electrophoresis (Comet assay) was performed in lymphocytes of adult mice in the last experiment. Similarly, this result could be linked to a greater sensitivity of neonatal mice to radon emissions compared with intrauterine mice. Further investigations on the effects of radon and its decay products during the intrauterine life and the first neonatal period should be performed to better clarify its genotoxic activity. 展开更多
关键词 RADON GENOTOXICITY DNA damage micronucleus test comet assay.
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Hesperidin Reduces Cisplatin-lnduced DNA Damage in Bone Marrow Cells of Mice
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作者 Tatiane da Silva Passos Esdras Andrade Santana +3 位作者 Maina Mantovanelli da Mota Jean Carlos Vencioneck Dutra Juliana Macedo Delarmelina Maria do Carmo Pimentel Batitucci 《Journal of Pharmacy and Pharmacology》 2017年第5期282-288,共7页
Hesperidin is a bioflavonoid abundantly found in citrus fruits and displays chemoprotective effects against DNA (deoxyribonucleic acid) damage, however there are few reports about hesperidin effects against cisplati... Hesperidin is a bioflavonoid abundantly found in citrus fruits and displays chemoprotective effects against DNA (deoxyribonucleic acid) damage, however there are few reports about hesperidin effects against cisplatin-DNA damage induction. The aim of this work was to evaluate hesperidin antimutagenicity against cisptatin-DNA damage. (1) The antimutagenicity of hesperidin was assayed by bone marrow of mice in vivo using the micronucleus test. Hesperidin pre-treatment protocol reduced the frequency of MNPCE (micronucleated polychromatic erythrocytes) and the dose of 100 mg·kg-1 was highest efficiency, with 65.24% of damage reduction. In the simultaneous treatment protocol, the dose of 200 mg·kg-1 exhibited a more effective reduction of MNPCE, with 94.01% of damage reduction. (2) Hesperidin was also effective in reducing the MNPCE frequency in the post-treatment protocol for all doses, with 77.48%, 82.13% and 90.08% of damage reduction at the doses of 100, 200 and 400 mg·kg-1, respectively. From the study, it can be concluded that hesperidin was able to promote the reduction of micronuclei frequency and DNA damage induced by cisplatin. Hesperidin is a powerful antioxidant compound and its chemoprotective effects on DNA may occur due to its association with the antioxidant cell system which is responsible for eliminate free radicals generated by chemical harmful to DNA. 展开更多
关键词 HESPERIDIN micronucleus test DNA damage CHEMOPROTECTION antimutagenicity.
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Genotoxicity biomarkers in aquatic bioindicators 被引量:1
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作者 Claudia BOLOGNESI Silvia CIRILLO 《Current Zoology》 SCIE CAS CSCD 2014年第2期273-284,共12页
Pollution of the aquatic environment is an ever-growing problem, as waters are the ultimate sink for the large number of xenobiotics from multiple sources. DNA damaging agents have a significant ecological relevance s... Pollution of the aquatic environment is an ever-growing problem, as waters are the ultimate sink for the large number of xenobiotics from multiple sources. DNA damaging agents have a significant ecological relevance since they are implicated in many pathological processes and exert effects beyond that of individual being active through following generations. A large number of methods have been applied to evaluate genotoxic damage in different aquatic species. Comet assay, as method for de- tecting DNA alterations, and micronucleus test, as an index of chromosomal damage are the most widely applied and validated methods in field studies. These methods were applied in different vertebrate and invertebrate aquatic species, but only mollusk and fish species have been employed in routine biomonitoring programs. Mussels, due to their widely geographical distribution and the suitability for caging represent the bioindicator of choice in field studies. Mytilus species is the most used marine mussel. The use of fish is limited to specific geographic areas. The present review mainly focuses on the application of comet assay and micronucleus test in mussels. A number of biomonitoring studies in mussels, using comet assay or micronucleus test, revealed exposure to different classes of genotoxic compounds with a good discrimination power. The different evidence from the two as- says, reflects different biological mechanisms for the two genetic endpoints, DNA damage and chromosomal damage, suggesting their combined application in the field. Different endogenous and exogenous factors have been shown to modulate the genotoxic responses in mussels, acting as confounding factors in environmental monitoring. The use of standardized protocol for caging, sampling and genotoxity evaluation is critical in biomonitoring studies. The use of a multimarker approach coupling genotoxicity biomarkers with physiological and biochemical factors allows to have a complete picture of the environmental pollution [Current Zoology 60 (2): 273-284, 2014]. 展开更多
关键词 Aquatic animals GENOTOXICITY BIOINDICATOR Biomarker micronucleus test Comet assay BIVALVE MUSSEL
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