Two microsateUite DNA loci were used to trace the pedigree structure of six families in the shrimp Fenneropenaeus chinensis. Four of the families were natural mating, and the others were mated by artificial inseminati...Two microsateUite DNA loci were used to trace the pedigree structure of six families in the shrimp Fenneropenaeus chinensis. Four of the families were natural mating, and the others were mated by artificial insemination. Eleven alleles were acquired at two microsatellite DNA loci （locus RS0622 and locus EN0033 ） by investigating 145 offsprings and 11 parents. Five alleles were acquired from locus RS0622 and six from locus EN0033. As analyzed, the gene frequencies were between 0. 024 1 and 0. 493 1, the heterozygosity was 0.652 2 and 0.688 8, and the polymorphism information content （PIC） was 0.585 7 and 0.652 9 for the locus RS0622 and the locus EN0033, respectively. Twenty-three genotypes were detected and the genotypes of the losing parents were also inferred. The pedigrees of three F1 and three F2 generations were determined by matching the genotype at each locus.展开更多
A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length...A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.展开更多
基金the National High Technology Development Project of China under contract No. 2005AA603210 the National Natural Science Foundation of China under contract No. 30500378.
文摘Two microsateUite DNA loci were used to trace the pedigree structure of six families in the shrimp Fenneropenaeus chinensis. Four of the families were natural mating, and the others were mated by artificial insemination. Eleven alleles were acquired at two microsatellite DNA loci （locus RS0622 and locus EN0033 ） by investigating 145 offsprings and 11 parents. Five alleles were acquired from locus RS0622 and six from locus EN0033. As analyzed, the gene frequencies were between 0. 024 1 and 0. 493 1, the heterozygosity was 0.652 2 and 0.688 8, and the polymorphism information content （PIC） was 0.585 7 and 0.652 9 for the locus RS0622 and the locus EN0033, respectively. Twenty-three genotypes were detected and the genotypes of the losing parents were also inferred. The pedigrees of three F1 and three F2 generations were determined by matching the genotype at each locus.
基金Supported by the National Science and Technology Support Plan Project(2012BDA25B10)the Science and Technology Project of Heilongjiang Province(GZ11B201)+1 种基金the Fundamental Research Fund for Central Public Welfare Research Institutes(201109)the Open Project of Key Laboratory of Freshwater Aquatic Biotechnology and Breeding,Ministry of Agriculture(2012NYBSJ-03)
文摘A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.
