Microsatellite Marker-based Analysis of the Genetic Diversity of Yellow River Carp

To provide accurate base data about the genetic sourees of Yellow River carps, the genetic diversity in a^ficially bred population and wild population of Yellow River carps from Henan Province was analyzed with mierosatellite markers. The results showed that 16 alleles were detected at six microsateUite loci in each population. The average effective number of alleles （Ne） was 2. 350 in artificially bred population and 2. 085 in wild population. The observed heterozygosity （Ho） of artificially bred population, wild population and mixed population was 0. 614, 0. 576 and 0. 601 ; and the unbiased expected heterozygesity （ He ） was 0. 569, 0.535 and 0.559 ; and the polymorphism infonnatian content （PlC） was 0.474, 0.428 and 0.468, respectively. The PIC of the six loci ranged from 0.304 to 0. 864. The analysis of the genetic differentiation for the six microsatellitc loci in the two populations showed that the genetic differentiation coefficient （ F,, ） at only one microsatellite locus HLJ483 was greater than 0.05, and that at five rnicrosatellite loci were less than 0.05, which was consistent with the standard of non- genetic differentiation between populations （F,, = 0 -0.05）. The average F,, at the six loci was 0.02, and the gene flow value （Nm） at all loci was greater than 1 and the average of Nm was 12.202. The results indicate that there is relatively abundant genetic diversity in Yellow River carps.

Parentage determination of black sea bream(Acanthopagrus schlegelii)for stock enhancement:effectiveness and loss of genetic variation

The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in China for a fewyears.However,little information has been reported concerning the effectiveness and genetic effect of black sea bream stock enhancement.In order to detect the contribution of released individuals in Zhujiang River Estuary(ZRE)and Daya Bay(DB),six microsatellite markers were used to identify the hatchery-released individuals.In addition,this pedigree of hatchery populations(broodfish and hatchery-released offspring)was traced to detect the number of effective parents(N_(e)),the inbreeding coefficient and the decrease of genetic variability in the reproduction.The pedigree reconstruction showed that at least 69(out of 93)broodfish had offspring.The estimated N_(e) was 54.8,consequently the inbreeding coefficient was 0.91%.The genetic diversity of hatchery-released offspring was lower than that in that of broodfish(heterozygosity alleles,0.727-0.774),some alleles(number of alleles,61-69)and genetic variance were lost during reproduction.It was observed that wild samples had higher levels of genetic diversity compared with hatchery populations as well as recaptured samples in releasing area.A total of 128 hatchery-released black sea bream were identified among 487 recaptured samples in ZRE,while a total of 15 samples were identified among 96 samples in DB.In summary,there was a high survival of released fish.N_(e)vertheless,the results provided evidence to consider a loss of genetic variation in hatcheryreleased stock and a negative genetic effect of the stock enhancement.