The Grainyhead-like 3（GRHL3） is involved in epidermal barrier formation, neural tube closure and wound repair. Previous studies have suggested that GRHL3 has been linked to many different types of cancers. However, ...The Grainyhead-like 3（GRHL3） is involved in epidermal barrier formation, neural tube closure and wound repair. Previous studies have suggested that GRHL3 has been linked to many different types of cancers. However, to date, its effects on human colorectal cancer（CRC） has not been clarified yet. Our microarray analysis has indicated predominant GRHL3 expression in CRC. The purpose of this study was to investigate the expression and significance of GRHL3 in CRC tumorigenesis using CRC tissues and paired paracancerous tissues, as well as using distinct CRC cell lines（HT29 and DLD1）. We observed increased GRHL3 expression at both m RNA and protein levels in CRC tissues and CRC cell lines using quantitative real-time polymerase chain reaction（q RT-PCR） and Western blotting. Moreover, silencing GRHL3 with si RNA could suppress CRC cell proliferation, viability and migration in vitro. We also found that knockdown of GRHL3 could promote cell cycle arrest at G0/G1 phase in HT29 cells and DLD1 cells, and induce cell apoptosis in HT29 cells. Together, our study revealed the down-regulation of GRHL3 in vitro could inhibit CRC cell activity and trigger cell cycle arrest at G0/G1 phase and apoptosis.展开更多
[Objective]The research aimed to study HCH persistence in Jiaozhou Bay.[Method]Based on the investigation data in Jiaozhou Bay in April,August,September and October of 1989,we analyzed the source,distribution and migr...[Objective]The research aimed to study HCH persistence in Jiaozhou Bay.[Method]Based on the investigation data in Jiaozhou Bay in April,August,September and October of 1989,we analyzed the source,distribution and migration status of HCH in sea area of Jiaozhou Bay.By using structural model of HCH environmental background value in Jiaozhou Bay,basic background value,input amount of terrestrial runoff and environmental background value of HCH in Jiaozhou Bay were calculated.[Result]Basic background value of HCH in water area of Jiaozhou Bay was 0.012 7μg/L,HCH input amount of terrestrial runoff was during 0-0.057 1μg/L,HCH content input by ocean current was 0μg/L.Then,environmental background value of HCH in water area of Jiaozhou Bay was during 0.012 7-0.069 8μg/L.[Conclusion]HCH needed long time migrating from land to sea bottom,and experienced terrestrial migration process,water migration process,settlement process and biological migration cycle process.In these migration processes,HCH was persistent,and always caused threats and harms on organism.展开更多
基金supported by grants from National Natural Science Foundation of China(No.81072152)Natural Science Foundation of Hubei Province(No.2015CFA027)+1 种基金Research Foundation of Health and Family Planning Commission of Hubei Province(No.WJ2015MA010 and No.WJ2017M249)Clinical Medical Research Center of Peritoneal Cancer of Wuhan(No.2015060911020462)
文摘The Grainyhead-like 3（GRHL3） is involved in epidermal barrier formation, neural tube closure and wound repair. Previous studies have suggested that GRHL3 has been linked to many different types of cancers. However, to date, its effects on human colorectal cancer（CRC） has not been clarified yet. Our microarray analysis has indicated predominant GRHL3 expression in CRC. The purpose of this study was to investigate the expression and significance of GRHL3 in CRC tumorigenesis using CRC tissues and paired paracancerous tissues, as well as using distinct CRC cell lines（HT29 and DLD1）. We observed increased GRHL3 expression at both m RNA and protein levels in CRC tissues and CRC cell lines using quantitative real-time polymerase chain reaction（q RT-PCR） and Western blotting. Moreover, silencing GRHL3 with si RNA could suppress CRC cell proliferation, viability and migration in vitro. We also found that knockdown of GRHL3 could promote cell cycle arrest at G0/G1 phase in HT29 cells and DLD1 cells, and induce cell apoptosis in HT29 cells. Together, our study revealed the down-regulation of GRHL3 in vitro could inhibit CRC cell activity and trigger cell cycle arrest at G0/G1 phase and apoptosis.
基金Supported by Key Laboratory Item of Colleges and Universities,Guizhou Department of Education,China([2012]003)Research Conditions Help Project of Guizhou High-level Talent,China(TZJF-2011-44)+3 种基金Support Plan Program for Excellent Talents in New Century,Ministry of Education,China(NCET-12-0659)Governor Capital Fund for Excellent Education Talent in Guizhou,China([2012]71)Guiyang Low-carbon Science and Technology Plan Project,China(2012205)Research Fund of Director in North China Sea Environmental Monitoring Center of State Oceanic Administration,China(05EMC16)
文摘[Objective]The research aimed to study HCH persistence in Jiaozhou Bay.[Method]Based on the investigation data in Jiaozhou Bay in April,August,September and October of 1989,we analyzed the source,distribution and migration status of HCH in sea area of Jiaozhou Bay.By using structural model of HCH environmental background value in Jiaozhou Bay,basic background value,input amount of terrestrial runoff and environmental background value of HCH in Jiaozhou Bay were calculated.[Result]Basic background value of HCH in water area of Jiaozhou Bay was 0.012 7μg/L,HCH input amount of terrestrial runoff was during 0-0.057 1μg/L,HCH content input by ocean current was 0μg/L.Then,environmental background value of HCH in water area of Jiaozhou Bay was during 0.012 7-0.069 8μg/L.[Conclusion]HCH needed long time migrating from land to sea bottom,and experienced terrestrial migration process,water migration process,settlement process and biological migration cycle process.In these migration processes,HCH was persistent,and always caused threats and harms on organism.
