Due to their important biological role as markers for different pathologies, sialic acid （SA） analyses are important for clinical research. In this work, a miniaturized capillary electrophoresis with amperometrie de...Due to their important biological role as markers for different pathologies, sialic acid （SA） analyses are important for clinical research. In this work, a miniaturized capillary electrophoresis with amperometrie detection （mini-CE-AD） was developed for the determination of N-aeetylneuraminic acid （NANA）, which is the most widespread form of SAs. NANA was first oxidized by periodic acid in an acidic solution, and then the oxidation product β-formyl pyruvic acid was derivatized with electroactive 2-thiobarbituric acid （TBA） to form an electroactive NANA-TBA adduct, which could be readily determined by mini-CE-AD. The limit of detection （LOD） of NANA-TBA could achieve 0.50 μg/mL （1.6 μmol·L-1, S/N=3） based on an online enrichment approach of moving chemical reaction boundary. The proposed method was successfully applied to the analysis of NANA in human saliva, and the recoveries were in the range of 91.8%-109% with RSDs of 1.8%-3.9%. Due to its simple design and construction, low cost and portability, the mini-CE-AD device will possess more practicability in more field work as an alternative to conventional and microchip CE approaches.展开更多
基金This work was supported by the National Natural Science Foundation of China （No. 21205042）, the Daxia Foundation of East China Normal University （No. 2015DX-284） and the Students Innovative Experimental Project of Shanghai Municipality （No. 201610269070）. The authors have declared no conflicts of interest.
文摘Due to their important biological role as markers for different pathologies, sialic acid （SA） analyses are important for clinical research. In this work, a miniaturized capillary electrophoresis with amperometrie detection （mini-CE-AD） was developed for the determination of N-aeetylneuraminic acid （NANA）, which is the most widespread form of SAs. NANA was first oxidized by periodic acid in an acidic solution, and then the oxidation product β-formyl pyruvic acid was derivatized with electroactive 2-thiobarbituric acid （TBA） to form an electroactive NANA-TBA adduct, which could be readily determined by mini-CE-AD. The limit of detection （LOD） of NANA-TBA could achieve 0.50 μg/mL （1.6 μmol·L-1, S/N=3） based on an online enrichment approach of moving chemical reaction boundary. The proposed method was successfully applied to the analysis of NANA in human saliva, and the recoveries were in the range of 91.8%-109% with RSDs of 1.8%-3.9%. Due to its simple design and construction, low cost and portability, the mini-CE-AD device will possess more practicability in more field work as an alternative to conventional and microchip CE approaches.
