目的:探讨寻常型银屑病中医证型与血清miR-155、转化生长因子β1(TGF-β1)、银屑病皮损面积及严重程度指数(PASI)评分相关性研究。方法:回顾性选取2021年9月~2023年9月北京中医药大学东直门医院收治120例的寻常型银屑病患者为研究对象...目的:探讨寻常型银屑病中医证型与血清miR-155、转化生长因子β1(TGF-β1)、银屑病皮损面积及严重程度指数(PASI)评分相关性研究。方法:回顾性选取2021年9月~2023年9月北京中医药大学东直门医院收治120例的寻常型银屑病患者为研究对象。统计寻常型银屑病中医证型分布情况,比较不同中医证型患者血清miR-155、TGF-β1水平及PASI评分,分析寻常型银屑病中医证型与血清miR-155、TGF-β1水平与PASI评分的关系。结果:120例寻常型银屑病患者中血热证51例、血瘀证38例、血燥证31例;不同中医证型寻常型银屑病患者血清miR-155、TGF-β1水平及PASI评分为血热证>血瘀证>血燥证( P <0.05);寻常型银屑病患者血清miR-155、TGF-β1水平与PASI评分呈正相关(r=0.649、 P <0.05,r=0.676、 P <0.05)。结果:寻常型银屑病中医证型与血清miR-155、TGF-β1水平及PASI评分具有良好的相关性,可以为寻常型银屑病患者辨证施治提供指导。展开更多
Objective: This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regulators of biological behavior in Systemic Sclerosis. Methods: A total of 10 SSc patients and 10 healthy cont...Objective: This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regulators of biological behavior in Systemic Sclerosis. Methods: A total of 10 SSc patients and 10 healthy controls were selected for the study. The expression levels of hsa-miR-155-3p and hsa-miR-155-5p in peripheral blood mononuclear cells of SSc patients and healthy controls were measured using RT-qPCR. The diagnostic value of these miRNAs was explored using Receiver Operating Characteristic curve analysis. Pearson or Spearman correlation analysis was performed to assess the correlation between miRNAs and clinical indicators in SSc patients. Potential target genes of hsa-miR-155-3p and hsa-miR-155-5p were predicted using miRDB, Targetscan, and miRDIP databases. GO functional annotation, KEGG pathway enrichment analysis, protein-protein interaction network construction, and selection of central genes were conducted. Results: The expression levels of hsa-miR-155-3p and hsa- miR-155-5p were significantly higher in PBMCs of SSc patients compared to healthy controls (P<0.001). The ROC curve analysis showed that hsa-miR-155-3p and hsa-miR-155-5p had a high diagnostic value for SSc (AUC=1, P<0.001). Correlation analysis revealed that hsa- miR-155-3p, hsa-miR-155-5p, and clinical indicators such as high-resolution CT, neutrophil percentage, lymphocyte percentage, and albumin to globulin ratio were correlated (P<0.05). The signaling pathways enriched with target genes of hsa-miR-155-3p and hsa-miR-155- 5p were closely associated with the occurrence and development of SSc fibrosis, immunity, and inflammation. Conclusions: hsa-miR-155-3p and hsa-miR-155-5p may be involved in regulating the occurrence and development of SSc fibrosis, immunity, and inflammation. They have the potential to serve as biomarkers for clinical diagnosis and treatment of SSc.展开更多
文摘目的:探讨寻常型银屑病中医证型与血清miR-155、转化生长因子β1(TGF-β1)、银屑病皮损面积及严重程度指数(PASI)评分相关性研究。方法:回顾性选取2021年9月~2023年9月北京中医药大学东直门医院收治120例的寻常型银屑病患者为研究对象。统计寻常型银屑病中医证型分布情况,比较不同中医证型患者血清miR-155、TGF-β1水平及PASI评分,分析寻常型银屑病中医证型与血清miR-155、TGF-β1水平与PASI评分的关系。结果:120例寻常型银屑病患者中血热证51例、血瘀证38例、血燥证31例;不同中医证型寻常型银屑病患者血清miR-155、TGF-β1水平及PASI评分为血热证>血瘀证>血燥证( P <0.05);寻常型银屑病患者血清miR-155、TGF-β1水平与PASI评分呈正相关(r=0.649、 P <0.05,r=0.676、 P <0.05)。结果:寻常型银屑病中医证型与血清miR-155、TGF-β1水平及PASI评分具有良好的相关性,可以为寻常型银屑病患者辨证施治提供指导。
基金National Natural Science Foundation of China(No.8186029481860295)Natural Science Foundation of Inner Mongolia Autonomous Region(No.2019MS080552021MS08045)Science and Technology Plan Project of Inner Mongolia Autonomous Region(No.2018020892019GG052)。
文摘Objective: This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regulators of biological behavior in Systemic Sclerosis. Methods: A total of 10 SSc patients and 10 healthy controls were selected for the study. The expression levels of hsa-miR-155-3p and hsa-miR-155-5p in peripheral blood mononuclear cells of SSc patients and healthy controls were measured using RT-qPCR. The diagnostic value of these miRNAs was explored using Receiver Operating Characteristic curve analysis. Pearson or Spearman correlation analysis was performed to assess the correlation between miRNAs and clinical indicators in SSc patients. Potential target genes of hsa-miR-155-3p and hsa-miR-155-5p were predicted using miRDB, Targetscan, and miRDIP databases. GO functional annotation, KEGG pathway enrichment analysis, protein-protein interaction network construction, and selection of central genes were conducted. Results: The expression levels of hsa-miR-155-3p and hsa- miR-155-5p were significantly higher in PBMCs of SSc patients compared to healthy controls (P<0.001). The ROC curve analysis showed that hsa-miR-155-3p and hsa-miR-155-5p had a high diagnostic value for SSc (AUC=1, P<0.001). Correlation analysis revealed that hsa- miR-155-3p, hsa-miR-155-5p, and clinical indicators such as high-resolution CT, neutrophil percentage, lymphocyte percentage, and albumin to globulin ratio were correlated (P<0.05). The signaling pathways enriched with target genes of hsa-miR-155-3p and hsa-miR-155- 5p were closely associated with the occurrence and development of SSc fibrosis, immunity, and inflammation. Conclusions: hsa-miR-155-3p and hsa-miR-155-5p may be involved in regulating the occurrence and development of SSc fibrosis, immunity, and inflammation. They have the potential to serve as biomarkers for clinical diagnosis and treatment of SSc.
