用蛋白酶K消化法提取萧氏松茎象总DNA,并对细胞色素C氧化酶亚基Ⅰ(cytochrome c oxidaseⅠgene,COⅠ)序列分析发现,COⅠ序列4种核苷酸量平均为A=30.2%,C=17.3%,G=15.4%,T=37.0%。将所获得的5个不同地区萧氏松茎象个体COⅠ序列排序后,比...用蛋白酶K消化法提取萧氏松茎象总DNA,并对细胞色素C氧化酶亚基Ⅰ(cytochrome c oxidaseⅠgene,COⅠ)序列分析发现,COⅠ序列4种核苷酸量平均为A=30.2%,C=17.3%,G=15.4%,T=37.0%。将所获得的5个不同地区萧氏松茎象个体COⅠ序列排序后,比较形成了一个含1 209个位点的矩阵,其中1 174个不变位点,35个变异位点,所占比例为2.89%,序列的A+T含量较高。遗传距离、核酸和氨基酸变异及系统发育树分析表明:萧氏松茎象与树皮象属亲缘关系最近,且分子水平上萧氏松茎象与树皮象属之间较之树皮象属内种间无明显差异,对目前的分类地位提出质疑。展开更多
采用PCR技术扩增6个地理种群桑天牛的线粒体细胞色素氧化酶Ⅰ（mt DNA COⅠ）基因序列,基于mt DNA COⅠ基因序列变异探讨不同地理分布桑天牛种群间的遗传距离、系统发育以及遗传分化程度。对6个地理种群的33个桑天牛样本的mt DNA COⅠ...采用PCR技术扩增6个地理种群桑天牛的线粒体细胞色素氧化酶Ⅰ（mt DNA COⅠ）基因序列,基于mt DNA COⅠ基因序列变异探讨不同地理分布桑天牛种群间的遗传距离、系统发育以及遗传分化程度。对6个地理种群的33个桑天牛样本的mt DNA COⅠ基因扩增产物的测序结果表明共有19个位点发生变异,占序列总长的4.0%,其中来自河南省济源的桑天牛有12个变异位点,来自浙江省的桑天牛有9个变异位点,来自河北省4个地区的桑天牛的变异位点较少;河北省的4个地理种群的遗传距离较小,在0.001 3-0.002 6之间,河北、河南、浙江种群间的遗传距离较大,其中河南种群与河北种群的遗传距离又远远大于浙江种群与河北种群的遗传距离,后2大地理种群分布区域的海拔更接近。利用MEGA4.1软件构建的NJ系统进化树显示,6个地理种群形成河北、河南和浙江3大地理分布格局;从遗传分化程度上来看,桑天牛种群的3大地理分布格局不存在共享的单元型,遗传分化系数（FST）在0.804 1-0.892 2范围内,遗传分化程度较高。综合分析认为,不同地理种群桑天牛存在分化的原因除地理分布外,更重要的是生态条件的差异。展开更多
Bemisia tabaci is a cryptic species complex, causing signiifcant loss on many agricultural y important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving ...Bemisia tabaci is a cryptic species complex, causing signiifcant loss on many agricultural y important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving sustainable pest management strategies. Here we investigate the whitelfy species complex in soybean in major soybean growing states of India. The mitochondrial cytochrome oxidase gene subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian methods indicated the existence of three cryptic species namely Asia I, Asia II 1, and Asia II 7. Al the haplotypes detected in the study could be assigned to these three cryptic species fol owing the species demarcation criteria of 3.5%divergence threshold. Of these, Asia II 1 was found to be predominant with wide spread distribution across the surveyed regions from cool temperate zones to hot and humid tropical plains. On the contrary, cryptic species Asia II 7 showed localized distribu-tion. The Asia II 1 exhibited the highest haplotype diversity and Asia I showed high level of nucleotide diversity. There was a signiifcantly high genetic differentiation among these three cryptic species. The MEAM 1, a dreadful invasive species was not detected in the specimens tested in the current study. The diversity and distribution of three cryptic species is discussed in the light of current knowledge on distribution of whitelfy species in India and yel ow mosaic disease observed during sampling survey.展开更多
基金This work was supported by National Natural Science Foundation of China (No. 30570247)the Natural Science Foundation of Shanxi Province (No. 2003-1087)
文摘用蛋白酶K消化法提取萧氏松茎象总DNA,并对细胞色素C氧化酶亚基Ⅰ(cytochrome c oxidaseⅠgene,COⅠ)序列分析发现,COⅠ序列4种核苷酸量平均为A=30.2%,C=17.3%,G=15.4%,T=37.0%。将所获得的5个不同地区萧氏松茎象个体COⅠ序列排序后,比较形成了一个含1 209个位点的矩阵,其中1 174个不变位点,35个变异位点,所占比例为2.89%,序列的A+T含量较高。遗传距离、核酸和氨基酸变异及系统发育树分析表明:萧氏松茎象与树皮象属亲缘关系最近,且分子水平上萧氏松茎象与树皮象属之间较之树皮象属内种间无明显差异,对目前的分类地位提出质疑。
文摘采用PCR技术扩增6个地理种群桑天牛的线粒体细胞色素氧化酶Ⅰ（mt DNA COⅠ）基因序列,基于mt DNA COⅠ基因序列变异探讨不同地理分布桑天牛种群间的遗传距离、系统发育以及遗传分化程度。对6个地理种群的33个桑天牛样本的mt DNA COⅠ基因扩增产物的测序结果表明共有19个位点发生变异,占序列总长的4.0%,其中来自河南省济源的桑天牛有12个变异位点,来自浙江省的桑天牛有9个变异位点,来自河北省4个地区的桑天牛的变异位点较少;河北省的4个地理种群的遗传距离较小,在0.001 3-0.002 6之间,河北、河南、浙江种群间的遗传距离较大,其中河南种群与河北种群的遗传距离又远远大于浙江种群与河北种群的遗传距离,后2大地理种群分布区域的海拔更接近。利用MEGA4.1软件构建的NJ系统进化树显示,6个地理种群形成河北、河南和浙江3大地理分布格局;从遗传分化程度上来看,桑天牛种群的3大地理分布格局不存在共享的单元型,遗传分化系数（FST）在0.804 1-0.892 2范围内,遗传分化程度较高。综合分析认为,不同地理种群桑天牛存在分化的原因除地理分布外,更重要的是生态条件的差异。
基金Department of Biotechnology, Government of India for liberal funding (Functional Genomics of Yellow Mosaic Viruses of Soybean and Development of Transgenic Resistance in Soybean: BT/PR9631/AGR/02/468/2007)
文摘Bemisia tabaci is a cryptic species complex, causing signiifcant loss on many agricultural y important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving sustainable pest management strategies. Here we investigate the whitelfy species complex in soybean in major soybean growing states of India. The mitochondrial cytochrome oxidase gene subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian methods indicated the existence of three cryptic species namely Asia I, Asia II 1, and Asia II 7. Al the haplotypes detected in the study could be assigned to these three cryptic species fol owing the species demarcation criteria of 3.5%divergence threshold. Of these, Asia II 1 was found to be predominant with wide spread distribution across the surveyed regions from cool temperate zones to hot and humid tropical plains. On the contrary, cryptic species Asia II 7 showed localized distribu-tion. The Asia II 1 exhibited the highest haplotype diversity and Asia I showed high level of nucleotide diversity. There was a signiifcantly high genetic differentiation among these three cryptic species. The MEAM 1, a dreadful invasive species was not detected in the specimens tested in the current study. The diversity and distribution of three cryptic species is discussed in the light of current knowledge on distribution of whitelfy species in India and yel ow mosaic disease observed during sampling survey.
