It was reported that activities of the mitochondrial H+-ATPase reconstituted on liposomes, performed by cholate dialysis method in the presence of 1 mM Mg2+ in the dialysis medium, were markedly increased and the resu...It was reported that activities of the mitochondrial H+-ATPase reconstituted on liposomes, performed by cholate dialysis method in the presence of 1 mM Mg2+ in the dialysis medium, were markedly increased and the results were easily reproducible. It was also suggested that Mg2+ may play a role in leading to a suitable conformation of the reconstituted H+-ATPase with higher activities by changing fluidity of phaspholipid bilayers of the reconstituted vesicles. It was recently reported that a certain amount of Mg2+ present in the dialysis medium is necessary for forming a proper fluidity of phospholipids of the proteoliposomes containing H+-ATPase. It was suggested that the circular dichroism spectrum may be used to measure the conformational展开更多
文摘It was reported that activities of the mitochondrial H+-ATPase reconstituted on liposomes, performed by cholate dialysis method in the presence of 1 mM Mg2+ in the dialysis medium, were markedly increased and the results were easily reproducible. It was also suggested that Mg2+ may play a role in leading to a suitable conformation of the reconstituted H+-ATPase with higher activities by changing fluidity of phaspholipid bilayers of the reconstituted vesicles. It was recently reported that a certain amount of Mg2+ present in the dialysis medium is necessary for forming a proper fluidity of phospholipids of the proteoliposomes containing H+-ATPase. It was suggested that the circular dichroism spectrum may be used to measure the conformational
