Atherosclerosis is a multiple-pathogeny disease that consists of many complicated processes,including oxidative stress,endothelium dysfunction,abnormal cell proliferation,apoptosis and extracellular matrix disorder.Th...Atherosclerosis is a multiple-pathogeny disease that consists of many complicated processes,including oxidative stress,endothelium dysfunction,abnormal cell proliferation,apoptosis and extracellular matrix disorder.The pivotal proteases for these courses are NAD(P)H oxidase,eNOS,MAPKs and MMPs,respectively.As an upstream kinase,protein kinase C(PKC) may involve in the development of atherosclerosis through affecting these enzymes.展开更多
目的探讨肉桂醛对UVA照射后体外皮肤成纤维细胞表达基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-3（MMP-3）和丝裂原活化蛋白激酶（mitogen—activated protein kinases，MAPK）的影响。方法皮肤成纤维细胞在体外培养后，在培养基中加...目的探讨肉桂醛对UVA照射后体外皮肤成纤维细胞表达基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-3（MMP-3）和丝裂原活化蛋白激酶（mitogen—activated protein kinases，MAPK）的影响。方法皮肤成纤维细胞在体外培养后，在培养基中加入不同浓度（0，5，10，20和40μM）肉桂醛，24h后MTT法检测细胞活性。皮肤成纤维细胞经肉桂醛预处理24h后再予UVA照射，Westernblot法检测MMP-1，MMP-3，ERK，JNK，p38磷酸化蛋白水平。结果0—40μM肉桂醛对成纤维细胞的活性无明显影响（P均〉0．05）。肉桂醛能抑制UVA照射后成纤维细胞MAPK信号蛋白ERK，JNK，p38的活化，其也能抑制成纤维细胞表达MMP-1和MMP-3，且各浓度间作用差异均有统计学意义（P均〈0．05）。结论肉桂醛可能通过抑制UVA照射后成纤维细胞MAPK信号蛋白的活化来抑制MMP-1和MMP-3的表达，减少胶原降解，延缓皮肤光老化。展开更多
文摘Atherosclerosis is a multiple-pathogeny disease that consists of many complicated processes,including oxidative stress,endothelium dysfunction,abnormal cell proliferation,apoptosis and extracellular matrix disorder.The pivotal proteases for these courses are NAD(P)H oxidase,eNOS,MAPKs and MMPs,respectively.As an upstream kinase,protein kinase C(PKC) may involve in the development of atherosclerosis through affecting these enzymes.
文摘目的探讨肉桂醛对UVA照射后体外皮肤成纤维细胞表达基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-3（MMP-3）和丝裂原活化蛋白激酶（mitogen—activated protein kinases，MAPK）的影响。方法皮肤成纤维细胞在体外培养后，在培养基中加入不同浓度（0，5，10，20和40μM）肉桂醛，24h后MTT法检测细胞活性。皮肤成纤维细胞经肉桂醛预处理24h后再予UVA照射，Westernblot法检测MMP-1，MMP-3，ERK，JNK，p38磷酸化蛋白水平。结果0—40μM肉桂醛对成纤维细胞的活性无明显影响（P均〉0．05）。肉桂醛能抑制UVA照射后成纤维细胞MAPK信号蛋白ERK，JNK，p38的活化，其也能抑制成纤维细胞表达MMP-1和MMP-3，且各浓度间作用差异均有统计学意义（P均〈0．05）。结论肉桂醛可能通过抑制UVA照射后成纤维细胞MAPK信号蛋白的活化来抑制MMP-1和MMP-3的表达，减少胶原降解，延缓皮肤光老化。
