为满足某大型海洋平台模块钢结构焊接的需要,采用SMAW和SAW焊接工艺对S420MLO材料进行焊接试验和力学性能试验。在试验过程中,通过裂纹尖端张开位移(Crack Tip Opening Displacement,CTOD)试验进行焊接工艺评定。试验结果表明,在焊材选...为满足某大型海洋平台模块钢结构焊接的需要,采用SMAW和SAW焊接工艺对S420MLO材料进行焊接试验和力学性能试验。在试验过程中,通过裂纹尖端张开位移(Crack Tip Opening Displacement,CTOD)试验进行焊接工艺评定。试验结果表明,在焊材选用合理、焊接工艺得到严格控制的情况下,通过焊前预热和焊后消氢处理,能获得良好的焊接接头性能,满足相关标准及项目文件的要求,避免对大型钢结构进行焊后热处理。展开更多
Based on both cDNA sequence of barley powdery mildew resistance control element Mlo and DNA sequence of the known putative disease resistance gene from Triticum monococcum L., we designed some primers to amplify resis...Based on both cDNA sequence of barley powdery mildew resistance control element Mlo and DNA sequence of the known putative disease resistance gene from Triticum monococcum L., we designed some primers to amplify resistant homologous sequences in the near isogenic lines (NILs) of powdery mildew resistance using RT-PCR method. Two expressed cDNA fragments were isolated from wheat genome. One showed 83% homology to the Mlo gene of barley. The other contained two possible open reading frames (ORFs). NBS conservative domains 2, 3 of disease resistance gene and 13 LRR structures similar to rice Pib protein terminal were found respectively in the two ORFs. It indicated that the latter fragment belongs to NBS-LRR-like genes. The obvious difference of RT-PCR products was observed between the before challenged and the challenged for 72 h by Blumeria graminis f. sp. tritici, which, implied that this sequence could be associated with disease resistance of wheat. Using nulli-tetrasomic lines of 'Chinese Spring', the NBS-LRR-like gene had been located on chromosome 1D.展开更多
文摘为满足某大型海洋平台模块钢结构焊接的需要,采用SMAW和SAW焊接工艺对S420MLO材料进行焊接试验和力学性能试验。在试验过程中,通过裂纹尖端张开位移(Crack Tip Opening Displacement,CTOD)试验进行焊接工艺评定。试验结果表明,在焊材选用合理、焊接工艺得到严格控制的情况下,通过焊前预热和焊后消氢处理,能获得良好的焊接接头性能,满足相关标准及项目文件的要求,避免对大型钢结构进行焊后热处理。
文摘Based on both cDNA sequence of barley powdery mildew resistance control element Mlo and DNA sequence of the known putative disease resistance gene from Triticum monococcum L., we designed some primers to amplify resistant homologous sequences in the near isogenic lines (NILs) of powdery mildew resistance using RT-PCR method. Two expressed cDNA fragments were isolated from wheat genome. One showed 83% homology to the Mlo gene of barley. The other contained two possible open reading frames (ORFs). NBS conservative domains 2, 3 of disease resistance gene and 13 LRR structures similar to rice Pib protein terminal were found respectively in the two ORFs. It indicated that the latter fragment belongs to NBS-LRR-like genes. The obvious difference of RT-PCR products was observed between the before challenged and the challenged for 72 h by Blumeria graminis f. sp. tritici, which, implied that this sequence could be associated with disease resistance of wheat. Using nulli-tetrasomic lines of 'Chinese Spring', the NBS-LRR-like gene had been located on chromosome 1D.
