The electrochemical behavior of Mn (Ⅲ)/Mn(Ⅱ)ion-pair on platinum electrode in acid media were studied by cyclic voltammetry.It was demonstrated that the redox process of Mn(Ⅲ)/Mn(Ⅱ) pair was a simple pseudo-revers...The electrochemical behavior of Mn (Ⅲ)/Mn(Ⅱ)ion-pair on platinum electrode in acid media were studied by cyclic voltammetry.It was demonstrated that the redox process of Mn(Ⅲ)/Mn(Ⅱ) pair was a simple pseudo-reversible one-electron reaction between Mn(Ⅲ) and Mn(Ⅱ).The electrochemical kinetics of the redox reaction on static Pt electrode was a mass transfer controlled one.The calculated diffusion coefficient of Mn(Ⅱ) was 1.48×10 -6cm 2/s.展开更多
The separation and determination of captopril in human serum and urine samples by means of high performance liquid chromatography(HPLC) with on-line electrogenerated Mn(Ⅲ) chemiluminescence detection was carried out....The separation and determination of captopril in human serum and urine samples by means of high performance liquid chromatography(HPLC) with on-line electrogenerated Mn(Ⅲ) chemiluminescence detection was carried out. The method was based on the direct chemiluminescence reaction between captopril and Mn(Ⅲ), which was on-line electrogenerated by constant current electrolysis. The chromatographic separation was performed on a nucleosil RP-C18(250 mm×4.6mm i.d., 5 μm) column with an isocratic mobile phase consisting of acetonitrile-1% aqueous acetic acid(volume ratio 6∶4) at a flow-rate of 1.2 mL/min. The temperature was 25 ℃. The effects of several parameters on the HPLC resolution and CL emission were studied systematically. Under the optimal conditions, the linear range and detection limit for captopril are 5—800 ng/mL and 0.9 ng/mL, respectively. The relative standard derivation for 10.0 ng/mL captopril is 2.2%(n=11). The average recoveries for captopril in human serum and urine samples ranged from 94.8% to 103.2%, and the relative standard deviations of the quantitative results are below 3.4%. The proposed method had been applied to the determination of captopril in human serum and urine samples.展开更多
文摘The electrochemical behavior of Mn (Ⅲ)/Mn(Ⅱ)ion-pair on platinum electrode in acid media were studied by cyclic voltammetry.It was demonstrated that the redox process of Mn(Ⅲ)/Mn(Ⅱ) pair was a simple pseudo-reversible one-electron reaction between Mn(Ⅲ) and Mn(Ⅱ).The electrochemical kinetics of the redox reaction on static Pt electrode was a mass transfer controlled one.The calculated diffusion coefficient of Mn(Ⅱ) was 1.48×10 -6cm 2/s.
文摘The separation and determination of captopril in human serum and urine samples by means of high performance liquid chromatography(HPLC) with on-line electrogenerated Mn(Ⅲ) chemiluminescence detection was carried out. The method was based on the direct chemiluminescence reaction between captopril and Mn(Ⅲ), which was on-line electrogenerated by constant current electrolysis. The chromatographic separation was performed on a nucleosil RP-C18(250 mm×4.6mm i.d., 5 μm) column with an isocratic mobile phase consisting of acetonitrile-1% aqueous acetic acid(volume ratio 6∶4) at a flow-rate of 1.2 mL/min. The temperature was 25 ℃. The effects of several parameters on the HPLC resolution and CL emission were studied systematically. Under the optimal conditions, the linear range and detection limit for captopril are 5—800 ng/mL and 0.9 ng/mL, respectively. The relative standard derivation for 10.0 ng/mL captopril is 2.2%(n=11). The average recoveries for captopril in human serum and urine samples ranged from 94.8% to 103.2%, and the relative standard deviations of the quantitative results are below 3.4%. The proposed method had been applied to the determination of captopril in human serum and urine samples.