Molecular Tagging of a New Resistance Gene to Maize Dwarf Mosaic Virus Using Microsatellite Markers

With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene and polygene, and identified a new major gene. Bulked segregate and microsatellite analysis of a F 2 progeny from the combination of Huangzaosi×Mo17 were used to identify the resistance gene, mdm1(t), on the long arm of chromosome 6. This new resistance gene is tightly linked to and located between the microsatellite markers loci, phi077 and bnlg391. The linkage distances between phi077-mdm1(t) and mdm1(t)-bnlg391 are 4.74 centiMorgan (cM) and 6.72 cM respectively.

Molecular tagging techniques and their applications to the study of complex thermal flow phenomena

This review article reports the recent progress in the development of a new group of molecule-based flow diagnostic techniques, which include molecular tag- ging velocimetry （MTV） and molecular tagging thermometry （MTT）, for both qualitative flow visualization of thermally induced flow structures and quantitative whole-field mea- surements of flow velocity and temperature distributions. The MTV and MTT techniques can also be easily combined to result in a so-called molecular tagging velocimetry and ther- mometry （MTV＆T） technique, which is capble of achieving simultaneous measurements of flow velocity and temperature distribution in fluid flows. Instead of using tiny particles, the molecular tagging techniques （MTV, MTT, and MTV＆T） use phosphorescent molecules, which can be turned into long-lasting glowing marks upon excitation by photons of appropriate wavelength, as the tracers for the flow veloc- ity and temperature measurements. The unique attraction and implementation of the molecular tagging techniques are demonstrated by three application examples, which include： （1） to quantify the unsteady heat transfer process from a heated cylinder to the surrounding fluid flow in order to exam- ine the thermal effects on the wake instabilities behind the heated cylinder operating in mixed and forced heat convec- tion regimes, （2） to reveal the time evolution of unsteady heat transfer and phase changing process inside micro-sized, icing water droplets in order to elucidate the underlying physics pertinent to aircraft icing phenomena, and （3） to achievesimultaneous droplet size, velocity and temperature measure- ments of ＂in-flight＂ droplets to characterize the dynamic and thermodynamic behaviors of flying droplets in spray flows.