目的:观察中药尿酸利仙(含药血清)对尿酸盐诱导的人血管内皮细胞(Endothelial cell of vessels,ECV)血管细胞黏附分子-1(VCAM-1)mRNA表达的影响。方法:①体外培养人血管内皮细胞,用终浓度为1070μmol/L的尿酸盐溶液刺激,同时用低、中、...目的:观察中药尿酸利仙(含药血清)对尿酸盐诱导的人血管内皮细胞(Endothelial cell of vessels,ECV)血管细胞黏附分子-1(VCAM-1)mRNA表达的影响。方法:①体外培养人血管内皮细胞,用终浓度为1070μmol/L的尿酸盐溶液刺激,同时用低、中、高浓度(2.5%、5%、10%)尿酸利仙含药血清的培养液进行干预,用逆转录-聚合酶链式反应(RT-PCR)技术测定细胞VCAM-1的mRNA表达。结果:尿酸盐可诱导血管内皮细胞VCAM-1的mRNA高表达,差异有极显著的统计学意义(P<0.01);各浓度尿酸利仙含药血清干预组细胞VCAM-1的mRNA表达均较尿酸盐刺激组下降,差异有统计学意义。结论:尿酸利仙含药血清可抑制VCAM-1过度表达,是减轻尿酸盐诱导的人血管内皮细胞炎症损伤及抑制炎症反应的机制之一。展开更多
Various bio-inspired dry adhesive materials have been investigated.In this work,lithography and silicon deep etching method were used to fabricate bio-inspired micro-pillar dry adhesive materials of which the paramete...Various bio-inspired dry adhesive materials have been investigated.In this work,lithography and silicon deep etching method were used to fabricate bio-inspired micro-pillar dry adhesive materials of which the parameters,such as side length,Height to Side length Aspect Ratio(HSAR),and inter-pillar Distance to Side length Aspect Ratio(DSAR)were comprehensively studied to obtain a dry adhesive with high adhesion.An orthogonal array method was designed and a series of fabrication experiments were conducted to identify optimum parameters,which resulted in a high normal adhesion of 2.98 N·cm^-2 and a shear adhesion of 9.59 N·cm^-2.An adhesion and desorption device was further designed on basis of the optimum dry adhesive,which enables an Unmanned Aerial Vehicle(UAV)to successfully adhere on and detach from a ceiling.This would allow an UAV to stay aloft for prolonged time,which could be invaluable to many applications,such as energy conservation and aerial detection.展开更多
文摘目的:观察中药尿酸利仙(含药血清)对尿酸盐诱导的人血管内皮细胞(Endothelial cell of vessels,ECV)血管细胞黏附分子-1(VCAM-1)mRNA表达的影响。方法:①体外培养人血管内皮细胞,用终浓度为1070μmol/L的尿酸盐溶液刺激,同时用低、中、高浓度(2.5%、5%、10%)尿酸利仙含药血清的培养液进行干预,用逆转录-聚合酶链式反应(RT-PCR)技术测定细胞VCAM-1的mRNA表达。结果:尿酸盐可诱导血管内皮细胞VCAM-1的mRNA高表达,差异有极显著的统计学意义(P<0.01);各浓度尿酸利仙含药血清干预组细胞VCAM-1的mRNA表达均较尿酸盐刺激组下降,差异有统计学意义。结论:尿酸利仙含药血清可抑制VCAM-1过度表达,是减轻尿酸盐诱导的人血管内皮细胞炎症损伤及抑制炎症反应的机制之一。
基金The authors gratefully acknowledge the National Natural Science Foundation of China(No.51605220)Jiangsu Province Natural Science Foundation(No.BK20160793)the High Level Introduction of Talent Research Start-up Fund in NUAA(No.1011-YAH16010)。
文摘Various bio-inspired dry adhesive materials have been investigated.In this work,lithography and silicon deep etching method were used to fabricate bio-inspired micro-pillar dry adhesive materials of which the parameters,such as side length,Height to Side length Aspect Ratio(HSAR),and inter-pillar Distance to Side length Aspect Ratio(DSAR)were comprehensively studied to obtain a dry adhesive with high adhesion.An orthogonal array method was designed and a series of fabrication experiments were conducted to identify optimum parameters,which resulted in a high normal adhesion of 2.98 N·cm^-2 and a shear adhesion of 9.59 N·cm^-2.An adhesion and desorption device was further designed on basis of the optimum dry adhesive,which enables an Unmanned Aerial Vehicle(UAV)to successfully adhere on and detach from a ceiling.This would allow an UAV to stay aloft for prolonged time,which could be invaluable to many applications,such as energy conservation and aerial detection.
