AIM To investigate whether morin can reduce hepatic fibrosis by activating the NF-E2-related factor 2(Nrf2) signaling pathway.METHODS Twenty male Sprague-Dawley rats were randomly divided into four groups: control gro...AIM To investigate whether morin can reduce hepatic fibrosis by activating the NF-E2-related factor 2(Nrf2) signaling pathway.METHODS Twenty male Sprague-Dawley rats were randomly divided into four groups: control group, morin group, carbon tetrachloride(CCl4) group, and morin + CCl4 group. Rats in both the CCl4 and morin + CCl4 groups were injected intraperitoneally with CCl4 at a dose of 2 mL/kg twice a week. Rats in both the morin and morin + CCl4 groups were treated orally with morin at a dose of 50 mg/kg twice a week. Control rats were treated with vehicle only twice a week. At the end-point of the 8 wk of the experimental period, serum AST, ALT, and ALP were measured, and the liver specimenswere obtained for pathological assessment. Real-time PCR and Western blot methods were used to analyze the expression of α-smooth muscle actin(α-SMA), collagen Ⅰ, collagen Ⅲ, Nrf2, heme oxygenase(HO-1), and quinone oxidoreductase 1(NQO1) using frozen liver specimens.RESULTS Morin-treated rats in the morin + CCl4 group had less hyperplasia of fiber tissue, minimal inflammatory cells, and less body weight loss with favorable liver enzyme measurements compared to rats treated with CCl4 only. Additionally, morin-treated rats had significantly lower m RNA and protein expression of α-SMA, collagen Ⅰ, and collagen Ⅲ, but significantly higher m RNA and protein expression of Nrf2, HO-1, and NQO1 compared to rats treated with CCl4 only(P < 0.05).CONCLUSION Morin could play a protective role by inducing the expression of Nrf2 and its downstream antioxidant factors(HO-1 and NQO1) and reducing the expression of α-SMA, collagen Ⅰ, and collagen Ⅲ in CCl4-induced liver fibrosis rats.展开更多
Objective:To determine the protective effect of morin, a flavonoid against deoxycorticosterone acetate(DOCA)-salt induced hypertension in male Wistar rats.Methods:Hypertension was induced in uninephrectomized rats by ...Objective:To determine the protective effect of morin, a flavonoid against deoxycorticosterone acetate(DOCA)-salt induced hypertension in male Wistar rats.Methods:Hypertension was induced in uninephrectomized rats by weekly twice subcutaneous injection of DOCA(25 mg/kg bw) and 1% NaCl in the drinking water for six consecutive weeks. Effect of morin against DOCA-salt induced hypertension was evaluated by measuring blood pressure and performing biochemical estimations and histopathological examination of renal tissues.Results:DOCA-salt hypertensive rats showed considerably increased systolic and diastolic blood pressure,serum hepatic marker enzyme activities such as aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP) and gamma-glutamyl transpeptidase(GGT)and renal function markers(urea, uric acid and creatinine) in plasma. Oral administration of morin(25, 50 and 75 mg/kg bw) brought back all the above parameters to near normal level.Histopathology of kidney also confirmed the biochemical findings of this study. The effect at a dose of 50 mg/kg bw of morin was more pronounced than that of the other two doses(25 and 75 mg/kg bw).Conclusions:These findings indicate that morin exhibits strong antihypertensive effect against DOCA-salt induced hypertension.展开更多
Objective: To evaluate the protective effect of morin against pentylenetetrazol(PTZ)-induced tonic-clonic convulsions in mice. Methods: Swiss albino mice(18-22 g) was used to induce convulsions by intraperitoneal(i.p....Objective: To evaluate the protective effect of morin against pentylenetetrazol(PTZ)-induced tonic-clonic convulsions in mice. Methods: Swiss albino mice(18-22 g) was used to induce convulsions by intraperitoneal(i.p.) administration of PTZ(90 mg/kg). Mice were either pretreated with morin(10, 20 and 40 mg/kg) or vehicle(distilled water, 10 mg/kg) 45 min before PTZ administration. Various behavioral and biochemical parameters were assessed. Results: PTZ administration resulted in significant production(P<0.001) of tonic-clonic conclusion and mortality in mice. PTZ-induced increase in the duration of convulsion, onset of convulsion and mortality was inhibited significantly by morin(20 and 40 mg/kg) administration. The PTZinduced decrease in brain GABA, dopamine and Na+K+ATPase levels and increase in xanthine oxidase activity were inhibited significantly by morin(20 and 40 mg/kg) treatment. The increased levels of malondialdehyde and nitric oxide level were significantly decreased by morin(20 and 40 mg/kg) treatment. Also, reduced levels of superoxide dismutase and glutathione were increased significantly by morin treatment. Conclusions: Results of the present study indicate that morin showed its anti-convulsant effect via modulating the levels of brain GABA, Na^+K^+ATPase, and oxido-nitrosative stress. Thus, morin can be a potential candidate for further clinical evaluations as an anti-epileptic agent.展开更多
Flavonols are plant nature. Morin and other related pigments that are ubiquitous in plant flavonols have come into recent prominence because of their usefulness as anticancer, antitumor, anti-AIDS, and other important...Flavonols are plant nature. Morin and other related pigments that are ubiquitous in plant flavonols have come into recent prominence because of their usefulness as anticancer, antitumor, anti-AIDS, and other important therapeutic activities of significant potency and low systemic toxicity. The heat of combustion of morin （molecular formula, C15H10O7·H2O） in oxygen was measured by a rotating-bomb type combustion calorimeter, the standard molar enthalpy of combustion of morin at T = 298.15 K was determined to be △cH^ m （C15H10O7·H2O, s） = - （5 937.99±2.99） kJ·mol^-1. The derived standard molar enthalpy of the formation of morin in solid powder state at T = 298.15 K, △fH^ m（C15H10O7·H2O, s）, was -（1 682.12 ± 3.58） kJ·mol^1, which provide an accurate data of the stability of morin to the pharmacy and pharmacology.展开更多
Objective:To explore the therapeutic role of morin against L-arginine-induced acute pancreatitis in rats.Methods:The group 1 received two intraperitoneal injections of normal saline,and groups 2-4 were given two intra...Objective:To explore the therapeutic role of morin against L-arginine-induced acute pancreatitis in rats.Methods:The group 1 received two intraperitoneal injections of normal saline,and groups 2-4 were given two intraperitoneal injections of L-arginine(250 mg/100 g body weight)at 1 h interval to induce acute pancreatitis.Subsequently,group 2 received no further treatment while groups 3 and 4 were treated with morin(30 mg/kg)and diclofenac sodium(30 mg/kg),respectively.Blood glucose and serum levels of insulin,α-amylase,malondialdehyde,myeloperoxidase,alanine aminotransferase,aspartate aminotransferase and cholesterol were measured.Moreover,histopathological study was carried out to investigate the effect of morin treatment on physiology of the pancreas.Results:L-arginine significantly altered the level of blood glucose and serum levels of insulin,α-amylase,malondialdehyde,myeloperoxidase,alanine aminotransferase,aspartate aminotransferase and cholesterol.Treatment with morin or diclofenac sodium significantly improved the levels of these biomarkers.Furthermore,morin showed more significant effect than diclofenac sodium.Histopathological analysis verified that morin protected the pancreas from deleterious effects of L-arginine.Conclusions:Morin plays a protective role against L-arginineinduced acute pancreatitis via reducing lipid peroxidation and tissue inflammation,and attenuating acute pancreatitis-associated alteration in insulin secretion and glucose metabolism.展开更多
Morin (MR) is an anticancer drug present in fruits and Chinese herbs. Fe3O4 magnetic nanoparticles (MNPs) coated on 3-aminopropyl triethoxysilane (APTES) were synthesized (MNPs-APTES) as carriers for MR. The character...Morin (MR) is an anticancer drug present in fruits and Chinese herbs. Fe3O4 magnetic nanoparticles (MNPs) coated on 3-aminopropyl triethoxysilane (APTES) were synthesized (MNPs-APTES) as carriers for MR. The characterization of drug delivery system was confirmed by Fourier Transform Infrared (FTIR), Transmission Electron Microscope (TEM), X-Ray Diffraction (XRD), dynamic light scattering (DLS), and vibrating sample magnetometer (VSM). The adsorbed APTES on the magnetite surface (MNPs-APTES) was examined by FTIR. The TEM image showed that the average particle size is obtained to be about 26.7 nm for MNPs-APTES. The MR loading and release behavior of MNPs-APTES were studied and the results showed that up to 60% of the adsorbed drug was released within 4 h. In summary, the MNPs-APTES nanocarriers are based on the results, promising for targeted morin drug delivery.展开更多
A highly sensitive and fairly selective spectrofluorimetric method has been developed for the determination of germanium with morin in tap water and health drink. The fluorescent reaction and optimal conditions of ger...A highly sensitive and fairly selective spectrofluorimetric method has been developed for the determination of germanium with morin in tap water and health drink. The fluorescent reaction and optimal conditions of germanium with morin in phosphoric acid medium were studied. The detection limits of germanium in tap water and health drink were found to be 0.2 and 0.7μd/L respectively.展开更多
THE magnetic property of the ultrafine particles differs from that ot the bulk materials and has a lot of applications. Because of the size effect, magnetically ordered materials exhibit both superparamagnetism and co...THE magnetic property of the ultrafine particles differs from that ot the bulk materials and has a lot of applications. Because of the size effect, magnetically ordered materials exhibit both superparamagnetism and collective magnetic excitation, and the magnetic transition temperature changes in the ultrafine particles with respect to the bulk materials as well. Bulk α-Fe<sub>2</sub>O<sub>3</sub> is weakly ferromagnetic above the Morin transition temperature T<sub>M</sub>展开更多
Ⅰ. INTRODUCTIONBy using extraction-spectrophotometry or extraction-fluorophotometry, some methods were utilized to study the complexes of rare earth ions with morin (MOR). The authors propose that the rare earth ions...Ⅰ. INTRODUCTIONBy using extraction-spectrophotometry or extraction-fluorophotometry, some methods were utilized to study the complexes of rare earth ions with morin (MOR). The authors propose that the rare earth ions form unstable complexes with morin only in weak acidic so-展开更多
The structure of penicillin sulfoxide is rearranged to cephalosporins by the Morin rearrangement. It is a unit reaction for the preparation of various types of cephalosporins. In order to make better use of the reacti...The structure of penicillin sulfoxide is rearranged to cephalosporins by the Morin rearrangement. It is a unit reaction for the preparation of various types of cephalosporins. In order to make better use of the reaction and in view of the shortage of the reaction theory, this study used m062x/6-311++G(d, p) to explore the possible ring-opening reaction of the penicillin sulfoxide. It is found that the isomer of(S)-sulfoxide is a necessary structure. At the same time, the intramolecular hydrogen bonding effect between the side-chain amide proton(-CONH-) and the sulfinyl oxygen(-SO) is the decisive structure factor for the formation of alkenyl in ring-opening reaction, and the best reaction path is S0-> TS2-> IN1 channel. The main effect of acid catalysis is to catalyze the dehydration reaction of sulfenic acid to form sulfur cations for subsequently ring closing reaction.展开更多
文摘AIM To investigate whether morin can reduce hepatic fibrosis by activating the NF-E2-related factor 2(Nrf2) signaling pathway.METHODS Twenty male Sprague-Dawley rats were randomly divided into four groups: control group, morin group, carbon tetrachloride(CCl4) group, and morin + CCl4 group. Rats in both the CCl4 and morin + CCl4 groups were injected intraperitoneally with CCl4 at a dose of 2 mL/kg twice a week. Rats in both the morin and morin + CCl4 groups were treated orally with morin at a dose of 50 mg/kg twice a week. Control rats were treated with vehicle only twice a week. At the end-point of the 8 wk of the experimental period, serum AST, ALT, and ALP were measured, and the liver specimenswere obtained for pathological assessment. Real-time PCR and Western blot methods were used to analyze the expression of α-smooth muscle actin(α-SMA), collagen Ⅰ, collagen Ⅲ, Nrf2, heme oxygenase(HO-1), and quinone oxidoreductase 1(NQO1) using frozen liver specimens.RESULTS Morin-treated rats in the morin + CCl4 group had less hyperplasia of fiber tissue, minimal inflammatory cells, and less body weight loss with favorable liver enzyme measurements compared to rats treated with CCl4 only. Additionally, morin-treated rats had significantly lower m RNA and protein expression of α-SMA, collagen Ⅰ, and collagen Ⅲ, but significantly higher m RNA and protein expression of Nrf2, HO-1, and NQO1 compared to rats treated with CCl4 only(P < 0.05).CONCLUSION Morin could play a protective role by inducing the expression of Nrf2 and its downstream antioxidant factors(HO-1 and NQO1) and reducing the expression of α-SMA, collagen Ⅰ, and collagen Ⅲ in CCl4-induced liver fibrosis rats.
基金financially supported by Department of Science and Technology,New Delhi,India(grant No.SR/FT/LS-150/2008)
文摘Objective:To determine the protective effect of morin, a flavonoid against deoxycorticosterone acetate(DOCA)-salt induced hypertension in male Wistar rats.Methods:Hypertension was induced in uninephrectomized rats by weekly twice subcutaneous injection of DOCA(25 mg/kg bw) and 1% NaCl in the drinking water for six consecutive weeks. Effect of morin against DOCA-salt induced hypertension was evaluated by measuring blood pressure and performing biochemical estimations and histopathological examination of renal tissues.Results:DOCA-salt hypertensive rats showed considerably increased systolic and diastolic blood pressure,serum hepatic marker enzyme activities such as aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP) and gamma-glutamyl transpeptidase(GGT)and renal function markers(urea, uric acid and creatinine) in plasma. Oral administration of morin(25, 50 and 75 mg/kg bw) brought back all the above parameters to near normal level.Histopathology of kidney also confirmed the biochemical findings of this study. The effect at a dose of 50 mg/kg bw of morin was more pronounced than that of the other two doses(25 and 75 mg/kg bw).Conclusions:These findings indicate that morin exhibits strong antihypertensive effect against DOCA-salt induced hypertension.
文摘Objective: To evaluate the protective effect of morin against pentylenetetrazol(PTZ)-induced tonic-clonic convulsions in mice. Methods: Swiss albino mice(18-22 g) was used to induce convulsions by intraperitoneal(i.p.) administration of PTZ(90 mg/kg). Mice were either pretreated with morin(10, 20 and 40 mg/kg) or vehicle(distilled water, 10 mg/kg) 45 min before PTZ administration. Various behavioral and biochemical parameters were assessed. Results: PTZ administration resulted in significant production(P<0.001) of tonic-clonic conclusion and mortality in mice. PTZ-induced increase in the duration of convulsion, onset of convulsion and mortality was inhibited significantly by morin(20 and 40 mg/kg) administration. The PTZinduced decrease in brain GABA, dopamine and Na+K+ATPase levels and increase in xanthine oxidase activity were inhibited significantly by morin(20 and 40 mg/kg) treatment. The increased levels of malondialdehyde and nitric oxide level were significantly decreased by morin(20 and 40 mg/kg) treatment. Also, reduced levels of superoxide dismutase and glutathione were increased significantly by morin treatment. Conclusions: Results of the present study indicate that morin showed its anti-convulsant effect via modulating the levels of brain GABA, Na^+K^+ATPase, and oxido-nitrosative stress. Thus, morin can be a potential candidate for further clinical evaluations as an anti-epileptic agent.
基金Supported by the National Natural Science Foundation of China (30570015, 20621502)the Natural Science Foundation of Hubei Prov-ince (2005ABC002)the Research Foundation of Chinese Ministry of Edu-cation ([2006]8-IRT0543)
文摘Flavonols are plant nature. Morin and other related pigments that are ubiquitous in plant flavonols have come into recent prominence because of their usefulness as anticancer, antitumor, anti-AIDS, and other important therapeutic activities of significant potency and low systemic toxicity. The heat of combustion of morin （molecular formula, C15H10O7·H2O） in oxygen was measured by a rotating-bomb type combustion calorimeter, the standard molar enthalpy of combustion of morin at T = 298.15 K was determined to be △cH^ m （C15H10O7·H2O, s） = - （5 937.99±2.99） kJ·mol^-1. The derived standard molar enthalpy of the formation of morin in solid powder state at T = 298.15 K, △fH^ m（C15H10O7·H2O, s）, was -（1 682.12 ± 3.58） kJ·mol^1, which provide an accurate data of the stability of morin to the pharmacy and pharmacology.
基金financially supported by the research grant(5661/Punjab/NRPU/R&D/HEC/2016,6429/Punjab/NRPU/R&D/HEC/2016 and 8365/Punjab/NRPU/R&D/HEC/2017)received from Higher Education Commission of Pakistan
文摘Objective:To explore the therapeutic role of morin against L-arginine-induced acute pancreatitis in rats.Methods:The group 1 received two intraperitoneal injections of normal saline,and groups 2-4 were given two intraperitoneal injections of L-arginine(250 mg/100 g body weight)at 1 h interval to induce acute pancreatitis.Subsequently,group 2 received no further treatment while groups 3 and 4 were treated with morin(30 mg/kg)and diclofenac sodium(30 mg/kg),respectively.Blood glucose and serum levels of insulin,α-amylase,malondialdehyde,myeloperoxidase,alanine aminotransferase,aspartate aminotransferase and cholesterol were measured.Moreover,histopathological study was carried out to investigate the effect of morin treatment on physiology of the pancreas.Results:L-arginine significantly altered the level of blood glucose and serum levels of insulin,α-amylase,malondialdehyde,myeloperoxidase,alanine aminotransferase,aspartate aminotransferase and cholesterol.Treatment with morin or diclofenac sodium significantly improved the levels of these biomarkers.Furthermore,morin showed more significant effect than diclofenac sodium.Histopathological analysis verified that morin protected the pancreas from deleterious effects of L-arginine.Conclusions:Morin plays a protective role against L-arginineinduced acute pancreatitis via reducing lipid peroxidation and tissue inflammation,and attenuating acute pancreatitis-associated alteration in insulin secretion and glucose metabolism.
文摘Morin (MR) is an anticancer drug present in fruits and Chinese herbs. Fe3O4 magnetic nanoparticles (MNPs) coated on 3-aminopropyl triethoxysilane (APTES) were synthesized (MNPs-APTES) as carriers for MR. The characterization of drug delivery system was confirmed by Fourier Transform Infrared (FTIR), Transmission Electron Microscope (TEM), X-Ray Diffraction (XRD), dynamic light scattering (DLS), and vibrating sample magnetometer (VSM). The adsorbed APTES on the magnetite surface (MNPs-APTES) was examined by FTIR. The TEM image showed that the average particle size is obtained to be about 26.7 nm for MNPs-APTES. The MR loading and release behavior of MNPs-APTES were studied and the results showed that up to 60% of the adsorbed drug was released within 4 h. In summary, the MNPs-APTES nanocarriers are based on the results, promising for targeted morin drug delivery.
文摘A highly sensitive and fairly selective spectrofluorimetric method has been developed for the determination of germanium with morin in tap water and health drink. The fluorescent reaction and optimal conditions of germanium with morin in phosphoric acid medium were studied. The detection limits of germanium in tap water and health drink were found to be 0.2 and 0.7μd/L respectively.
文摘THE magnetic property of the ultrafine particles differs from that ot the bulk materials and has a lot of applications. Because of the size effect, magnetically ordered materials exhibit both superparamagnetism and collective magnetic excitation, and the magnetic transition temperature changes in the ultrafine particles with respect to the bulk materials as well. Bulk α-Fe<sub>2</sub>O<sub>3</sub> is weakly ferromagnetic above the Morin transition temperature T<sub>M</sub>
基金Project supported by the National Natural Science Foundation of China.
文摘Ⅰ. INTRODUCTIONBy using extraction-spectrophotometry or extraction-fluorophotometry, some methods were utilized to study the complexes of rare earth ions with morin (MOR). The authors propose that the rare earth ions form unstable complexes with morin only in weak acidic so-
基金supported by Key Laboratories of Fine Chemicals and Surfactants in Sichuan Provincial Universities (2016JXY01)the Opening Project of Key Laboratory of Green Chemistry of Sichuan Institutes of Higher Education (LYJ1105)Sichuan University of Science and Engineering talent introduction fund (2017RCL19)。
文摘The structure of penicillin sulfoxide is rearranged to cephalosporins by the Morin rearrangement. It is a unit reaction for the preparation of various types of cephalosporins. In order to make better use of the reaction and in view of the shortage of the reaction theory, this study used m062x/6-311++G(d, p) to explore the possible ring-opening reaction of the penicillin sulfoxide. It is found that the isomer of(S)-sulfoxide is a necessary structure. At the same time, the intramolecular hydrogen bonding effect between the side-chain amide proton(-CONH-) and the sulfinyl oxygen(-SO) is the decisive structure factor for the formation of alkenyl in ring-opening reaction, and the best reaction path is S0-> TS2-> IN1 channel. The main effect of acid catalysis is to catalyze the dehydration reaction of sulfenic acid to form sulfur cations for subsequently ring closing reaction.
