The rhynchonellide brachiopod Dzieduszyckia Siemiradzki is widely distributed in the Lower Famennian strata,and exhibits a wide range of morphological variations among populations.1272 autochthonous Devonian Rongxian ...The rhynchonellide brachiopod Dzieduszyckia Siemiradzki is widely distributed in the Lower Famennian strata,and exhibits a wide range of morphological variations among populations.1272 autochthonous Devonian Rongxian Dzieduszyckia fossils were collected from three populations in South China,namely the Dazhai,Dalong,and Longmi sections.A series of quantitative morphological analyses were conducted to understand the morphological variation among populations and identify the triggers responsible for such variations.The results show that the Dazhai population differs significantly in morphospace from the Dalong and Longmi populations,while the latter two exhibit more similar morphological features,suggesting that geographic distribution may play a key role in affecting Dzieduszyckia morphology.The Longmi population shows a distinct morphospace from the Dazhai population and an overlapped morphospace with the Dalong population,indicating that habitat may not be the controlling factor.Lastly,the Dazhai and Dalong populations share some morphological similarities,but the Dazhai and Longmi populations occupy completely different morphospaces,indicating that evolution had a greater impact on phenotypic divergence than habitat.Our findings suggest that geographic distribution is the predominant factor influencing the morphological variation of Dzieduszyckia,while evolution and living habitat are less significant,the former of which might play a more important role.展开更多
Pretreatment is important for achieving high-value utilization of biomass.This study is conducted to evaluate the destruction of the Moso bamboo cell wall via hydrothermal pretreatment at different temperatures and pH...Pretreatment is important for achieving high-value utilization of biomass.This study is conducted to evaluate the destruction of the Moso bamboo cell wall via hydrothermal pretreatment at different temperatures and pH values.Compositional and morphological analyses and subsequent enzymatic hydrolysis of the solid fractions indicate that the destruction of the cell wall,instead of the degradation or removal of hemicellulose and lignin,or the configuration transition of the cellulose crystal structure,is the most critical aspect for improving bioconversion efficiency.Although only an 8%-10%weight loss is incurred and similar crystalline indexes are achieved after mild hydrothermal treatments,the recovery of glucose is doubled,whereas the recovery of xylose from pretreated samples is approximately 35%.展开更多
To compare the application of traditional morphometric methods(TMMs)and geometric morphometric methods(GMMs)in the study of intraspecific leaf morphological characters of Quercus dentata,fifteen linear measurement ind...To compare the application of traditional morphometric methods(TMMs)and geometric morphometric methods(GMMs)in the study of intraspecific leaf morphological characters of Quercus dentata,fifteen linear measurement indices and thirteen landmarks of leaves were used to study leaf morphology of three provenances(H1,H2,and H3).In TMMs,principal component analysis(PCA)showed that leaf size–related indices played an important role in population classification.Partial least square(PLS)analysis showed that the main morphological characters affecting leaf size were the average depth of the lobes and the length–width ratios.However,the important indices to distinguish the provenances were circularity,leaf width,and length–width ratio.The results of discriminant analysis(DA)showed that 74.0%of H1,68.0%of H2,and 74.0%of H3 were correctly classified.Cluster analysis showed that the Mahalanobis distances between H1 and H2,H1 and H3,and H2 and H3 were 4.3761,11.4629,and 10.2067,respectively.In GMMs,PCA based on symmetrical components showed that the difference in leaf morphology was mainly due to the changing trend of the leaf apex and base,petiole length,and degree of leaf cracking.PLS analysis showed that there was a significant covariation between the leaf symmetrical components and size:as the leaf enlarged,the widest part gradually moved up,and the shape changed from nearly oval to lanceolate.DA results showed that 86.0%and 78.0%of H1 and H2,70.0%and 80.0%of H1 and H3,and 82.0%and 76.0%of H2 and H3 were correctly classified.Canonical variate analysis results showed that the Mahalanobis distances between H1 and H2,H1 and H3,and H2 and H3 were 1.7238,1.5380,and 1.6329,respectively.Both GMMs and TMMs showed significant differences in morphology among the three Q.dentata provenances,but GMMs had higher classification accuracy and could provide more information about leaf shape,whereas TMMs could provide more information about leaf size.Based on our results,GMMs are promising in the study of leaf morphological variation within Q.dentata provenances.展开更多
Background:We summarize 5 cases of primary gliosarcoma with widespread extracranial metastases including our case.The glial components are eliminated due to the needs of the living environment in the process of parasi...Background:We summarize 5 cases of primary gliosarcoma with widespread extracranial metastases including our case.The glial components are eliminated due to the needs of the living environment in the process of parasitism and survival of brain glioma-sarcoma cells in lung metastasis.Methods:A PubMed search using the keywords"gliosarcoma"and"extracranial metastases"was performed followed by a review of cited literature.Our case was a 50-year-old female presented with headache and dizziness.MRI examination showed that there was a cystic solid tumor in the right temporal lobe.The tumor was removed totally.Seven months after the operation,the patient suffered recurrent intermittent headache.The resection for the recurrent tumor was performed.Postoperative pathology confirmed the recurrent gliosarcoma.A needle biopsy was performed for the nodular on the right lung.The lung tumor pathology suggested a sarcoma structure.Results:There was a female patient in five cases.The age range is 47 to 69 years old.The tumor recurred within a year.A combination of treatment modalities may extend survival;however,the prognosis remains poor.Conclusion:Primary gliosarcoma with extracranial metastases is extremely rare.Some findings uncovered an unexpected spatiotemporal morphological variation in the different foci of the same malignancy.展开更多
One hundred and twenty isolates of Bipolaris sorokiniana of wheat were isolated, identified and characterized based on morphological, cultural, pathogenic and ITS (Internal Transcribed Spacer) based molecular paramete...One hundred and twenty isolates of Bipolaris sorokiniana of wheat were isolated, identified and characterized based on morphological, cultural, pathogenic and ITS (Internal Transcribed Spacer) based molecular parameters. Collected isolates differed in respect of radial mycelial growth rate, color of the colony, surface texture of the colony, shape of the colony, conidia production ability and shape and color of conidia. ITS1 and ITS4 based PCR products yielded a species-specific band of 600 bp (base pairs) on agarose gel for the confirmation of B. sorokiniana. The mycelial growth rate of the isolates ranged from 1.39 to 4.46 mm/day. The septation of conidia ranged from 1.4 to 8.6. On the basis of conidia morphology, the isolates were grouped into five different groups, whereas the isolates were grouped into 12 cultural groups based on cultural characteristics. Cultural group 8 [CG (Cultural Group)-8] was the most virulent [38.5% LAD (Leaf area diseased)] and [CG (cultural Group)-4] was the less virulent [2% LAD (Leaf area diseased)] group among 12 groups when inoculated at tillering stage of wheat cv. Kachan.展开更多
Gracilariopsis lemaneiformis is a widely reported species of marine red algae, with records from around the world. Particularly in Mexico, it has been recorded along the coasts of the Gulf of Mexico and the Mexican Ca...Gracilariopsis lemaneiformis is a widely reported species of marine red algae, with records from around the world. Particularly in Mexico, it has been recorded along the coasts of the Gulf of Mexico and the Mexican Caribbean as well as the Tropical Mexican Pacific;so, its current distribution on the Mexican coasts is disjunct. From its molecular characterization with COI-5P and rbcL sequences of specimens collected at sites in Mexico where this species has been most frequently recorded, our aim was to re-evaluate the current distribution of Gp. lemaneiformis on the Mexican coasts and discuss the taxonomic implications. Phylogenetic analysis, supported by DNA species delimitation methods, genetic distances and morphological comparisons, showed that the current disjunct distribution of Gp. lemaniformis in Mexico is a consequence of taxonomic misidentifications. From our results, Gp. lemaneiformis is the only species of the genus with a distribution in the Tropical Mexican Pacific, whereas Gp. tenuifrons is the only species with a distribution in the Gulf of Mexico and the Mexican Caribbean. Also, we propose that Gp. cata-luziana is to be merged with Gp. tenuifrons, while Gp. costarisensis is to be merged with Gp. lemaneiformis.展开更多
Chinese cabbage(Brassica rapa ssp.pekinensis)is an economically important vegetable crop throughout the world,especially in Asia.High-quality genome sequences are available for Chinese cabbage,but gene functional stud...Chinese cabbage(Brassica rapa ssp.pekinensis)is an economically important vegetable crop throughout the world,especially in Asia.High-quality genome sequences are available for Chinese cabbage,but gene functional studies remain challenging.To promote functional genomic studies of Chinese cabbage,we generated an ethyl methane sulfonate(EMS)mutant population of~8000 M_(2) plants using the double haploid inbred line A03 as the parent.The genome of A03 was sequenced and used as a reference for high-throughput functional characterization of gene mutations at the whole-genome level.A total of 300 M_(2) to M_(5) EMS mutants were phenotypically screened and then sequenced,revealing 750629 SNPs and 46272 InDel mutations that cover 98.27%of all predicted genes in the A03 genome.A forward-genetics approach was successfully used to identify two genes with chloroplast-related functions that are responsible for the yellow leaf mutant trait.A reverse-genetics approach was also used to identify associations between mutations in five genes of the glucosinolate biosynthetic pathway and variations in glucosinolate content of the mutant plants.In addition,we built the Chinese cabbage EMS mutation database(CCEMD,www.bioinformaticslab.cn/EMSmutation/home)to increase the usability of this mutant population resource.In summary,we performed large-scale screening of a heading Chinese cabbage EMS mutant collection at the phenotypic and genotypic levels,which will facilitate gene mining of Chinese cabbage and might also be useful for the study of other Brassica crops.展开更多
基金the National Natural Science Foundation of China(Grant Nos.41772015 and 41672334)。
文摘The rhynchonellide brachiopod Dzieduszyckia Siemiradzki is widely distributed in the Lower Famennian strata,and exhibits a wide range of morphological variations among populations.1272 autochthonous Devonian Rongxian Dzieduszyckia fossils were collected from three populations in South China,namely the Dazhai,Dalong,and Longmi sections.A series of quantitative morphological analyses were conducted to understand the morphological variation among populations and identify the triggers responsible for such variations.The results show that the Dazhai population differs significantly in morphospace from the Dalong and Longmi populations,while the latter two exhibit more similar morphological features,suggesting that geographic distribution may play a key role in affecting Dzieduszyckia morphology.The Longmi population shows a distinct morphospace from the Dazhai population and an overlapped morphospace with the Dalong population,indicating that habitat may not be the controlling factor.Lastly,the Dazhai and Dalong populations share some morphological similarities,but the Dazhai and Longmi populations occupy completely different morphospaces,indicating that evolution had a greater impact on phenotypic divergence than habitat.Our findings suggest that geographic distribution is the predominant factor influencing the morphological variation of Dzieduszyckia,while evolution and living habitat are less significant,the former of which might play a more important role.
基金This study was supported by a grant from the Natural Science Foundation of China(No.31770622).
文摘Pretreatment is important for achieving high-value utilization of biomass.This study is conducted to evaluate the destruction of the Moso bamboo cell wall via hydrothermal pretreatment at different temperatures and pH values.Compositional and morphological analyses and subsequent enzymatic hydrolysis of the solid fractions indicate that the destruction of the cell wall,instead of the degradation or removal of hemicellulose and lignin,or the configuration transition of the cellulose crystal structure,is the most critical aspect for improving bioconversion efficiency.Although only an 8%-10%weight loss is incurred and similar crystalline indexes are achieved after mild hydrothermal treatments,the recovery of glucose is doubled,whereas the recovery of xylose from pretreated samples is approximately 35%.
基金supported by the National Key R&D Program of China during the 14th Five-year Plan Period(2021YFD2200302)the nonprofit industry research subject of the National Forestry and Grassland Administration in China(Grant Number 201504408)。
文摘To compare the application of traditional morphometric methods(TMMs)and geometric morphometric methods(GMMs)in the study of intraspecific leaf morphological characters of Quercus dentata,fifteen linear measurement indices and thirteen landmarks of leaves were used to study leaf morphology of three provenances(H1,H2,and H3).In TMMs,principal component analysis(PCA)showed that leaf size–related indices played an important role in population classification.Partial least square(PLS)analysis showed that the main morphological characters affecting leaf size were the average depth of the lobes and the length–width ratios.However,the important indices to distinguish the provenances were circularity,leaf width,and length–width ratio.The results of discriminant analysis(DA)showed that 74.0%of H1,68.0%of H2,and 74.0%of H3 were correctly classified.Cluster analysis showed that the Mahalanobis distances between H1 and H2,H1 and H3,and H2 and H3 were 4.3761,11.4629,and 10.2067,respectively.In GMMs,PCA based on symmetrical components showed that the difference in leaf morphology was mainly due to the changing trend of the leaf apex and base,petiole length,and degree of leaf cracking.PLS analysis showed that there was a significant covariation between the leaf symmetrical components and size:as the leaf enlarged,the widest part gradually moved up,and the shape changed from nearly oval to lanceolate.DA results showed that 86.0%and 78.0%of H1 and H2,70.0%and 80.0%of H1 and H3,and 82.0%and 76.0%of H2 and H3 were correctly classified.Canonical variate analysis results showed that the Mahalanobis distances between H1 and H2,H1 and H3,and H2 and H3 were 1.7238,1.5380,and 1.6329,respectively.Both GMMs and TMMs showed significant differences in morphology among the three Q.dentata provenances,but GMMs had higher classification accuracy and could provide more information about leaf shape,whereas TMMs could provide more information about leaf size.Based on our results,GMMs are promising in the study of leaf morphological variation within Q.dentata provenances.
文摘Background:We summarize 5 cases of primary gliosarcoma with widespread extracranial metastases including our case.The glial components are eliminated due to the needs of the living environment in the process of parasitism and survival of brain glioma-sarcoma cells in lung metastasis.Methods:A PubMed search using the keywords"gliosarcoma"and"extracranial metastases"was performed followed by a review of cited literature.Our case was a 50-year-old female presented with headache and dizziness.MRI examination showed that there was a cystic solid tumor in the right temporal lobe.The tumor was removed totally.Seven months after the operation,the patient suffered recurrent intermittent headache.The resection for the recurrent tumor was performed.Postoperative pathology confirmed the recurrent gliosarcoma.A needle biopsy was performed for the nodular on the right lung.The lung tumor pathology suggested a sarcoma structure.Results:There was a female patient in five cases.The age range is 47 to 69 years old.The tumor recurred within a year.A combination of treatment modalities may extend survival;however,the prognosis remains poor.Conclusion:Primary gliosarcoma with extracranial metastases is extremely rare.Some findings uncovered an unexpected spatiotemporal morphological variation in the different foci of the same malignancy.
文摘One hundred and twenty isolates of Bipolaris sorokiniana of wheat were isolated, identified and characterized based on morphological, cultural, pathogenic and ITS (Internal Transcribed Spacer) based molecular parameters. Collected isolates differed in respect of radial mycelial growth rate, color of the colony, surface texture of the colony, shape of the colony, conidia production ability and shape and color of conidia. ITS1 and ITS4 based PCR products yielded a species-specific band of 600 bp (base pairs) on agarose gel for the confirmation of B. sorokiniana. The mycelial growth rate of the isolates ranged from 1.39 to 4.46 mm/day. The septation of conidia ranged from 1.4 to 8.6. On the basis of conidia morphology, the isolates were grouped into five different groups, whereas the isolates were grouped into 12 cultural groups based on cultural characteristics. Cultural group 8 [CG (Cultural Group)-8] was the most virulent [38.5% LAD (Leaf area diseased)] and [CG (cultural Group)-4] was the less virulent [2% LAD (Leaf area diseased)] group among 12 groups when inoculated at tillering stage of wheat cv. Kachan.
基金supported by the projects:UAMI-CBS2019-2022:session 15.18-281118UAMI-CA-117,PRODEPcontains some results from the PhD project of Oscar E.Hernandez in the Doctorado en Ciencias Biologicas y de la Salud graduate program of the Universidad Autonoma Metropolitana.
文摘Gracilariopsis lemaneiformis is a widely reported species of marine red algae, with records from around the world. Particularly in Mexico, it has been recorded along the coasts of the Gulf of Mexico and the Mexican Caribbean as well as the Tropical Mexican Pacific;so, its current distribution on the Mexican coasts is disjunct. From its molecular characterization with COI-5P and rbcL sequences of specimens collected at sites in Mexico where this species has been most frequently recorded, our aim was to re-evaluate the current distribution of Gp. lemaneiformis on the Mexican coasts and discuss the taxonomic implications. Phylogenetic analysis, supported by DNA species delimitation methods, genetic distances and morphological comparisons, showed that the current disjunct distribution of Gp. lemaniformis in Mexico is a consequence of taxonomic misidentifications. From our results, Gp. lemaneiformis is the only species of the genus with a distribution in the Tropical Mexican Pacific, whereas Gp. tenuifrons is the only species with a distribution in the Gulf of Mexico and the Mexican Caribbean. Also, we propose that Gp. cata-luziana is to be merged with Gp. tenuifrons, while Gp. costarisensis is to be merged with Gp. lemaneiformis.
基金supported by the Innovative Research Group Project of Hebei Natural Science Foundation(grant no.C2020204111)the National Natural Science Foundation of China(grant nos.32002054,31902005,31930098,31972411,31722048,31872947)+7 种基金the International Science and Technology Cooperation base Special Project of Hebei(grant no.20592901D)Key R&D Project of Hebei(grant no.21326344D)the Natural Science Foundation of Hebei(grant nos.C2020204122,C2021204163)the Introducing Foreign Intellectual Project of Hebei(grant no.2019YX023A)the Post doctoral Science Foundation of China(grant no.2020M680901)the Science and Technology Research Project of University in Hebei(grant no.BJ2019020)the Post-graduate's Innovation Fund Project of Hebei(grant no.CXZZBS2017069)the State Key Laboratory of North China Crop Improvement and Regulation(NCCIR20202KF-2).
文摘Chinese cabbage(Brassica rapa ssp.pekinensis)is an economically important vegetable crop throughout the world,especially in Asia.High-quality genome sequences are available for Chinese cabbage,but gene functional studies remain challenging.To promote functional genomic studies of Chinese cabbage,we generated an ethyl methane sulfonate(EMS)mutant population of~8000 M_(2) plants using the double haploid inbred line A03 as the parent.The genome of A03 was sequenced and used as a reference for high-throughput functional characterization of gene mutations at the whole-genome level.A total of 300 M_(2) to M_(5) EMS mutants were phenotypically screened and then sequenced,revealing 750629 SNPs and 46272 InDel mutations that cover 98.27%of all predicted genes in the A03 genome.A forward-genetics approach was successfully used to identify two genes with chloroplast-related functions that are responsible for the yellow leaf mutant trait.A reverse-genetics approach was also used to identify associations between mutations in five genes of the glucosinolate biosynthetic pathway and variations in glucosinolate content of the mutant plants.In addition,we built the Chinese cabbage EMS mutation database(CCEMD,www.bioinformaticslab.cn/EMSmutation/home)to increase the usability of this mutant population resource.In summary,we performed large-scale screening of a heading Chinese cabbage EMS mutant collection at the phenotypic and genotypic levels,which will facilitate gene mining of Chinese cabbage and might also be useful for the study of other Brassica crops.