Gut microbes exhibit complex interactions with their hosts and shape an organism's immune system throughout its lifespan.As the largest secondary lymphoid organ,the spleen has a wide range of immunological functio...Gut microbes exhibit complex interactions with their hosts and shape an organism's immune system throughout its lifespan.As the largest secondary lymphoid organ,the spleen has a wide range of immunological functions.To explore the role of microbiota in regulating and shaping the spleen,we employ scRNA-seq and Stereo-seq technologies based on germ-free(GF)mice to detect differences in tissue size,anatomical structure,cell types,functions,and spatial molecular characteristics.We identify 18 cell types,9 subtypes of T cells,and 7 subtypes of B cells.Gene differential expression analysis reveals that the absence of microorganisms results in alterations in erythropoiesis within the red pulp region and congenital immune deficiency in the white pulp region.Stereo-seq results demonstrate a clear hierarchy of immune cells in the spleen,including marginal zone(MZ)macrophages,MZ B cells,follicular B cells and T cells,distributed in a well-defined pattern from outside to inside.However,this hierarchical structure is disturbed in GF mice.Ccr7 and Cxcl13 chemokines are specifically expressed in the spatial locations of T cells and B cells,respectively.We speculate that the microbiota may mediate the structural composition or partitioning of spleen immune cells by modulating the expression levels of chemokines.展开更多
Objective:To study the effects of Aidi Dripping Pills on immune functions of the tumor-bearing mouse on the basis of the previous experimental studies on its tumor-inhibiting and life-prolonging effects.Methods:By usi...Objective:To study the effects of Aidi Dripping Pills on immune functions of the tumor-bearing mouse on the basis of the previous experimental studies on its tumor-inhibiting and life-prolonging effects.Methods:By using the transplantation tumor mouse models,the effects of Aidi Dripping Pills on the lymphocyte transformation rate and the hemolysin formation in the S180 tumor-bearing mice,and on the phagocytic function of macrophages in the abdominal cavity of H22 tumor-bearing mice were investigated.Results:In the 2.25 g/kg and 1.125 g/kg Aidi Dripping Pills groups,the lymphocyte transformation rates in the S180 tumor-bearing mice were significantly higher than that of the control group(P<0.01).In all the Aidi Dripping Pills groups,HC50 significantly increased(P<0.01 or P<0.05),carbon granular clearance significantly raised,and both the phagocytic index and phagocytic coefficient were significantly higher than those in the control group(P<0.01 or P<0.05).Conclusion:The Aidi Dripping Pills can significantly increase the cellular immune function,the humoral immune function and the phagocytic function of the mononuclear-macrophages,so it may show anti-tumor effects by enhancing the function of the reticuloendothelial system.展开更多
基金funded by the National Natural Science Foundation of China(81700436)the Science Technology and Innovation Committee of Shenzhen Municipality,China(SGDX20190919142801722)。
文摘Gut microbes exhibit complex interactions with their hosts and shape an organism's immune system throughout its lifespan.As the largest secondary lymphoid organ,the spleen has a wide range of immunological functions.To explore the role of microbiota in regulating and shaping the spleen,we employ scRNA-seq and Stereo-seq technologies based on germ-free(GF)mice to detect differences in tissue size,anatomical structure,cell types,functions,and spatial molecular characteristics.We identify 18 cell types,9 subtypes of T cells,and 7 subtypes of B cells.Gene differential expression analysis reveals that the absence of microorganisms results in alterations in erythropoiesis within the red pulp region and congenital immune deficiency in the white pulp region.Stereo-seq results demonstrate a clear hierarchy of immune cells in the spleen,including marginal zone(MZ)macrophages,MZ B cells,follicular B cells and T cells,distributed in a well-defined pattern from outside to inside.However,this hierarchical structure is disturbed in GF mice.Ccr7 and Cxcl13 chemokines are specifically expressed in the spatial locations of T cells and B cells,respectively.We speculate that the microbiota may mediate the structural composition or partitioning of spleen immune cells by modulating the expression levels of chemokines.
文摘Objective:To study the effects of Aidi Dripping Pills on immune functions of the tumor-bearing mouse on the basis of the previous experimental studies on its tumor-inhibiting and life-prolonging effects.Methods:By using the transplantation tumor mouse models,the effects of Aidi Dripping Pills on the lymphocyte transformation rate and the hemolysin formation in the S180 tumor-bearing mice,and on the phagocytic function of macrophages in the abdominal cavity of H22 tumor-bearing mice were investigated.Results:In the 2.25 g/kg and 1.125 g/kg Aidi Dripping Pills groups,the lymphocyte transformation rates in the S180 tumor-bearing mice were significantly higher than that of the control group(P<0.01).In all the Aidi Dripping Pills groups,HC50 significantly increased(P<0.01 or P<0.05),carbon granular clearance significantly raised,and both the phagocytic index and phagocytic coefficient were significantly higher than those in the control group(P<0.01 or P<0.05).Conclusion:The Aidi Dripping Pills can significantly increase the cellular immune function,the humoral immune function and the phagocytic function of the mononuclear-macrophages,so it may show anti-tumor effects by enhancing the function of the reticuloendothelial system.
