Engineering DNA Materials for Sustainable Data Storage Using a DNA Movable-Type System

DNA molecules are green materials with great potential for high-density and long-term data storage.However,the current data-writing process of DNA data storage via DNA synthesis suffers from high costs and the production of hazards,limiting its practical applications.Here,we developed a DNA movable-type storage system that can utilize DNA fragments pre-produced by cell factories for data writing.In this system,these pre-generated DNA fragments,referred to herein as“DNA movable types,”are used as basic writing units in a repetitive way.The process of data writing is achieved by the rapid assembly of these DNA movable types,thereby avoiding the costly and environmentally hazardous process of de novo DNA synthesis.With this system,we successfully encoded 24 bytes of digital information in DNA and read it back accurately by means of high-throughput sequencing and decoding,thereby demonstrating the feasibility of this system.Through its repetitive usage and biological assembly of DNA movable-type fragments,this system exhibits excellent potential for writing cost reduction,opening up a novel route toward an economical and sustainable digital data-storage technology.

Enrichment factors of movable hydrocarbons in lacustrine shale oil and exploration potential of shale oil in Gulong Sag,Songliao Basin,NE China

The geological characteristics and production practices of the major middle-and high-maturity shale oil exploration areas in China are analyzed.Combined with laboratory results,it is clear that three essential conditions,i.e.economic initial production,commercial cumulative oil production of single well,and large-scale recoverable reserves confirmed by the testing production,determine whether the continental shale oil can be put into large-scale commercial development.The quantity and quality of movable hydrocarbons are confirmed to be crucial to economic development of shale oil,and focuses in evaluation of shale oil enrichment area/interval.The evaluation indexes of movable hydrocarbon enrichment include:(1)the material basis for forming retained hydrocarbon,including TOC>2%(preferentially 3%-4%),and typeⅠ-Ⅱkerogens;(2)the mobility of retained hydrocarbon,which is closely related to the hydrocarbon composition and flow behaviors of light/heavy components,and can be evaluated from the perspectives of thermal maturity(Ro),gas-oil ratio(GOR),crude oil density,quality of hydrocarbon components,preservation conditions;and(3)the reservoir characteristics associated with the engineering reconstruction,including the main pore throat distribution zone,reservoir physical properties(including fractures),lamellation feature and diagenetic stage,etc.Accordingly,13 evaluation indexes in three categories and their reference values are established.The evaluation indicates that the light shale oil zones in the Gulong Sag of Songliao Basin have the most favorable enrichment conditions of movable hydrocarbons,followed by light oil and black oil zones,containing 20.8×10^(8) t light oil resources in reservoirs with R_(0)>1.2%,pressure coefficient greater than 1.4,effective porosity greater than 6%,crude oil density less than 0.82 g/cm^(3),and GOR>100 m/m^(3).The shale oil in the Gulong Sag can be explored and developed separately by the categories(resource sweet spot,engineering sweet spot,and tight oil sweet spot)depending on shale oil flowability.The Gulong Sag is the most promising area to achieve large-scale breakthrough and production of continental shale oil in China.

SCATTERING FAR FIELD SOLUTION OF SH-WAVE BY MOVABLE RIGID CYLINDRICAL INTERFACE INCLUSION

The Green's function is used to solve the scattering far fieldsolution of SH-wave by a mov- able rigid cylindrical interfaceinclusion in a linear elastic body. First, a suitable Green'sfunction is devel- oped, which is the fundamental displacementsolution of an elastic half space with a movable rigid half-cylin-drical inclusion impacted by out-of-plane harmonic line source loadedat any point of its horizontal surface.

Wear Characteristics of the Nuclear Control Rod Drive Mechanism(CRDM)Movable Latch Serviced in High Temperature Water

The current research of nuclear control rod drive mechanism(CRDM)movable latch only makes a simple measurement of wear mass.The wear volume and difference in various claw surfaces are ignored and the degradation mechanism of each claw surface is not clear.In this paper,a detailed degradation analysis was carried out on each claw surface of movable latch combined with wear result and worn morphology.Results indicate that the boundary of carbide is preferred for corrosion because carbide presents a nobler Volta potential compared to the metal matrix or boundary region.Due to the oscillation of drive shaft between the claw surfaces of movable latch,the dominant wear mechanism on the upper surface of claw(USC)and lower surface of claw(LSC)is plastic deformation caused by impact wear.Mechanical impact wear will cause the fragmentation of carbides because of the high hardness and low ductility of carbides.Corrosion promotes the broken carbides to fall off from the metal matrix.The generated fine carbides(abrasive particles)cause extra abrasive wear on USC when the movable brings the drive shaft upward or downward.As a result,USC has a higher wear volume than LSC.This research proposes a method to evaluate the wear on the whole movable latches using a 3D full-size scanner.

Micro-accelerometer Based on Vertically Movable Gate Field Effect Transistor

A vertically movable gate field effect transistor(VMGFET) is proposed and demonstrated for a microaccelerometer application. The VMGFET using air gap as an insulator layer allows the gate to move on the substrate vertically by external forces. Finite element analysis is used to simulate mechanical behaviors of the designed structure. For the simulation, the ground acceleration spectrum of the 1952 Kern County Earthquake is employed to investigate the structural integrity of the sensor in vibration. Based on the simulation, a prototype VMGFET accelerometer is fabricated from silicon on insulator wafer. According to current–voltage characteristics of the prototype VMGFET, the threshold voltage is measured to be 2.32 V, which determines the effective charge density and the mutual transconductance of1.545910-8C cm-2and 6.59 m A V-1, respectively. The device sensitivity is 9.36–9.42 m V g-1in the low frequency,and the first natural frequency is found to be 1230 Hz. The profile smoothness of the sensed signal is in 3 d B range up to1 k Hz.

Distributed Connectivity Restoration Strategy for Movable Sensor Networks

Failure of one or multiple critical nodes may partition wireless sensor networks into disjoint segments, and thus brings negative effect on the applications. We propose DCRS, a Distributed Connectivity Restoration Strategy to tolerate the failure of one critical node. Because of the energy restriction of sensor nodes, the energy overhead of the recovery process should be minimized to extend the lifetime of the network. To achieve it, we first design a novel algorithm to identify 2-critical nodes only relying on the positional information of 1-hop neighbors and some 2-hop neighbors, and then we present the criteria to select an appropriate backup for each critical node. Finally, we improve the cascaded node movement algorithm by determining whether a node can move to another non-adjacent node directly or not to reduce the number of nodes moved. The effectiveness of DCRS is validated through extensive simulation experiments.

Dynamic anti-plane characteristic on an infinite piezoelectric medium with a movable rigid cylindrical inclusion

Scattering and dynamic stress concentrations of time harmonic SH-wave in an infinite elastic piezoelectric medium with a movable rigid cylindrical inclusion are studied in this paper with the help of complex variable and wave function expansion method. The relations that a movable rigid cylindrical inclusion depends on intensity of incident wave and electric field are revealed. The expressions of dynamic stress at the edge of the inclusion are obtained. Numerical calculations are made with different wave numbers and different piezoelectric characteristic parameters. The calculating results show that dynamic stress concentrations at the edge of the inclusion have linear dependence on the incident electric field. And dynamic analyses are very important for an infinite piezoelectric medium with a movable rigid cylindrical inclusion at larger piezoelectric characteristic parameters.

Relativistic electron scattering from freely movable proton/μ^+ in the presence of strong laser field

We have investigated the electron scattering from the freely movable spin-1/2 particle in the presence of a linearly polarized laser field in the first Born approximation.The laser-dressed state of electrons is described by a time-dependent wave function which is derived from a perturbation treatment.With the aids of numerical simulations, we explore the dependencies of the differential cross section on the laser field intensity as well as the electron-impact energy.Due to the mobility of the target, the differential cross section of this process is smaller than that of Mott scattering.

Modeling mechanical behaviors of composites with various ratios of matrixeinclusion properties using movable cellular automaton method

Two classes of composite materials are considered: classical metaleceramic composites with reinforcing hard inclusions as well as hard ceramics matrix with soft gel inclusions. Movable cellular automaton method is used for modeling the mechanical behaviors of such different heterogeneous materials. The method is based on particle approach and may be considered as a kind of discrete element method. The main feature of the method is the use of many-body forces of inter-element interaction within the formalism of simply deformable element approximation. It was shown that the strength of reinforcing particles and the width of particle-binder interphase boundaries had determining influence on the service characteristics of metaleceramic composite. In particular, the increasing of strength of carbide inclusions may lead to significant increase in the strength and ultimate strain of composite material. On the example of porous zirconia ceramics it was shown that the change in the mechanical properties of pore surface leads to the corresponding change in effective elastic modulus and strength limit of the ceramic sample. The less is the pore size, the more is this effect. The increase in the elastic properties of pore surface of ceramics may reduce its fracture energy.

Study of a Kit of GSM Radio Operator Site for Event-Driven Movable Coverage: Application to the Deployment of a Site of the Orange Operator in Ivory Coast

The mobile communication is nowadays one of the basic needs of humanity. It is essential to the flourishing of human beings. Considering this reality, the need to use its mobile phone is become more important and diversified. The subscribers of the various mobile telephone operators are increasingly demanding. This situation poses the problems of the cover mobile network to the operators and leads them to opt for several solutions and investments. The mobile operators in order to satisfy their customers use a policy of pushing the limits of network coverage in time and space for festive moments in targeted zones. Thus, we have conducted a study on the topic: study of a kit of GSM radio site for event-driven movable coverage. This work is applied to GSM (Global system mobile) network of the operator Orange-Ci, leader of mobile telephony in Ivory Coast. We thus proceeded under investigation initially of the various aspects of the ordinary sites (motionless radio site) which are already deployed with Orange-Ci in order to impregnate us infrastructures and equipment used. This study revealed us that a radio site comprises 4 parts: infrastructures, installations and energy equipments, installations and radio equipments, and installations and equipment of transmission. After the first analysis, we made a study of the movable site. The study of the movable site enabled us to see the various possible solutions to fulfill the basic functions of a movable radio site. After analysis we retained that our radio site will be built on a truck on which a mast of 25 m maximum length for the antennas will be embarked, it will be fed by a generator also embarked on the truck and the solution of transmission selected is the transmission by satellite more precisely technology VSAT. We choose the various equipments (radio, transmission, energy) according to features which we defined to constitute the kit of movable radio site.

A TWO-WAY INTERACTIVE MOVABLE NESTED MESH MODEL FOR TYPHOON TRACK PREDICTION

ＡＴＷＯ－ＷＡＹＩＮＴＥＲＡＣＴＩＶＥＭＯＶＡＢＬＥＮＥＳＴＥＤＭＥＳＨＭＯＤＥＬＦＯＲＴＹＰＨＯＯＮＴＲＡＣＫＰＲＥＤＩＣＴＩＯＮＷａｎｇＧｕｏｍｉｎ（王国民）（ＤｅｐａｒｔｍｅｎｔｏｆＡｔｍｏｓｐｈｅｒｉｃＳｃｉｅｎｃｅｓ，ＮａｎｊｉｎｇＵｎｉｖ...

Study on the Deformation Characteristics of the Sheet Hydroforming with a Movable Die

An improved sheet hydroforming process with a movable die is proposed and investigated by elastoplastic FEM. The effects of varied process parameters on the deformation of the sheet blanks were investigated. The effects of the movable die on the FLD were also analyzed. Using a movable die may increase the forming limit of the blank and improve the forming performance. Moreover, the drawing ratio of the blank may be increased by changing the process conditions such as blank holder force and the counterforce of the movable die on the blank.

Proposal for Offshore Wind Farm with Triangle-Coupled Movable Parallelogram Buoyancy Structure for the High Seas

We are responsible for providing energy and food resources for our next generation.After more than 20 years of research,the author has confirmed that the VAWT with flip-up mechanism can maintain rotation without the brake,even in strong winds.Experiments have shown that the triangularly coupled movable parallelogram buoyancy structure makes it easy to maintain the verticality of the wind turbine tower even under large waves.Furthermore,as a result of studying the connection between the HAWT(horizontal axis wind turbine)and the movable parallelogram buoyancy structure of the triangular connection,the feasibility was confirmed.The only way for all people in the world to secure energy and food fairly is to look for the remaining areas such as the high seas.The author would like to propose the use of the high seas to combat global hunger,which is likely to be a global problem in the future.

Integrated Movable System of Fuel Cell with Replaceable Fiber Bipolar Plate

It is important for the fuel cell integrated movable system to operate voltage and current using safety control technology. In order to work at the convenient condition of the fuel cell system, high performance fuel cell stack with replaceable fiber bipolar plate should be arranged with the integrated subsystem and appropriate working process. The parameters which affect the performance of PEMFC consisting of relative humidity, reaction temperature, gas inlet temperature, gas inlet pressure, and hydrogen and air flow rate. This study is to develop the integrated movable system on distributed power generation and backup power application, such as oxidant supply system, fuel supply system, heat management system, water management system, and power conditioning system. It comprises a novel PLC (Programmable Logic Control) system and human-machine interface. The controller is developed to control fuel cell system and record the operation data by using data acquisition system. The controller can be applied to high performance stack and system to obtain the best performance. The easy-taken high capacity hydrogen barrel embedded into steel plate of this movable system and more convenient than other fuel cell system.

FFSLCD的movable mura研究及改善

TFT-LCD中面板的显示不均现象一直是存在的,只是程度上各有轻重,此显示不均的现象我们统称为“mura”,其造成的原因和状态也是各不相同。按压movable mura主要是指触摸按压屏幕,mura随触摸位置移动,压力消失后mura不能恢复,低灰阶或关机状态下均明显可见。提高灰阶后,调回暗态mura也不可消失。此mura主要存在于IPS/FFS显示模式中。本文主要对此mura的产生机理进行探讨和实验论证。最终提出改善方向:提升IPS/FFS LCD显示面板的液晶量,优化PS设计。

Optimum Design of Movable Screen Jig Cycle

The basic method for designing and calculating reasonable jig cycle of movable screen jig was presented. The reasonabilityand reliability of designed jig cycle were verified by' means of the test on artificial mixed materials. An iron ore (12-100 mm) was treatedin tile TJD-75 model movable screen jig with the jig cycle. The good results were achieved. This would provide a reliable basis for designof commercial movable-screen jig.

STUDY ON GAP DISTRIBUTION IN MOVABLE SLEEVE-TOOTH DRIVE

A new method for calculating the gap distribution in the movable sleeve tooth transmission is presented. The non linear equations on analyzing the gap distribution are established with the method of equivalent mechanism and are solved successfully.

改变导叶翼型对水泵水轮机“S”特性的影响

针对水泵水轮机“S”特性区域的不稳定性问题,提出使用改型活动导叶翼型进行性能提升.以某水泵水轮机模型为研究对象,采用SST k-ω湍流模型对水泵水轮机全流道进行三维数值计算,通过对活动导叶翼型的改型设计,得出流量-转速模拟曲线,分析水泵水轮机组“S”特性改善情况.将计算与试验结果进行对比,并对改型活动导叶翼型前后机组的无叶区进行压力脉动分析.结果表明:在保证整体效率依然保持在92%附近的前提下,新的活动导叶翼型对机组的“S”特性依旧具有改善效果;无叶区压力脉动主要是受到活动导叶叶栅区域的分流在此重新聚集影响,并且与转轮叶片的动静相互扰动引起的;改型后的活动导叶降低了无叶区的压力脉动幅值,提升了水泵水轮机运行中的并网稳定性.

舌侧活动翼不同翼拉伸长度内收下前牙的生物力学特征

背景:舌侧活动翼矫治技术是一种新型舌侧矫治技术,不同翼的拉伸长度影响前牙转矩控制效果,目前尚无相关的生物力学研究。目的:探讨舌侧活动翼矫治技术内收下前牙时牙列的位移趋势以及不同翼拉伸长度对下颌前牙生物力学效应的影响。方法:利用锥形束CT采集患者下颌骨以及下牙列数据信息,通过Mimics软件进行三维重建,建立舌侧活动翼内收下前牙的三维有限元模型。在ANSYS软件中分别分析工况A(2 mm拉伸长度)、工况B(2.5 mm拉伸长度)、工况C(3 mm拉伸长度)和工况D(3.5 mm拉伸长度)时下颌前牙的初始位移。结果与结论:①下牙列初始位移趋势:中切牙舌向倾斜移动伴有压低,侧切牙、尖牙轻度舌侧移动伴近中舌向扭转,第二前磨牙远中倾斜并伴有明显舌倾,第一磨牙整体表现为近中倾斜并伴有近中牙冠外翻。因此在下颌拔牙内收病例中,对第二前磨牙的舌向移动应给予重视,临床中可以通过相应的技术予以抵消。②前牙各方向位移趋势:从工况A到工况D,矢状方向:中切牙冠根位移差值由-11.891μm变为-5.7574μm,表示中切牙由倾斜移动向整体移动转变;侧切牙冠根位移差值由-11.8281μm变为-6.71145μm,尖牙冠根位移差值由-7.5723μm变为-4.6955μm,表示侧切牙及尖牙的倾斜移动也在向整体移动转变。垂直方向:从工况A到工况D,切牙的压低量逐渐增大,尖牙的压低量逐渐减小。结果表明,翼的拉伸长度会影响前牙的倾斜移动趋势;随着翼的不断拉伸,下前牙的转矩控制效果越好。