Objective: To observe the effect of acumoxi (acupuncture and moxibustion) on macrophage (Mφ)-lL1-Th net-work and hydroperitoneum hepatoma (H 22) metastasis in mice. Methods: A total of 36 BALB/ c male mice bearing H ...Objective: To observe the effect of acumoxi (acupuncture and moxibustion) on macrophage (Mφ)-lL1-Th net-work and hydroperitoneum hepatoma (H 22) metastasis in mice. Methods: A total of 36 BALB/ c male mice bearing H 22 are randomly divided into control, acupuncture and acumoxi groups with 12 cases in each group. In the later 2 groups, Dazhui (GV 14) and Guanyuan (CV 4) are punctured once daily, continuously for 18 days, and in acumoxi group, the two acupoints were also moxibustioned alternatively with moxa stick once every day. After killing the mice, the tissue samples of the 3 groups are treated routinely step by step and analyzed by means of colorimetric analysis for determining the phagocytic function of the macrophages; and the content of IL1 of the Mφ supernatant is assayed with serum plate agglutination (SPA)-Ig floral hoop method of T helper cell (Th) monoclonal antibody; the weight of the reniportal lymph node, the kidney and the lung, and the number of the cancerous nodes on the pulmonary surface are calculated. Results: After acupuncture and moxibustion treatment, the immunoregulatory network indices of acumoxi group increase obviously compared with those of control group(P<0.01), showing an anti-metastasis effect of acumoxi on H 22. Conclusion: Results of the present study and those of our former research prove that acupuncture and moxibustion can suppress the tumor growth and H 22 metastasis by the enhancement of the immunoregulatory network.展开更多
Objective:to observe the clinical effect of acupuncture by mángzhen huid(relaxing needling with sharp long needle) on iliotibial band friction syndrome(ITBS).Design:this was a two-arm randomized controlled trial ...Objective:to observe the clinical effect of acupuncture by mángzhen huid(relaxing needling with sharp long needle) on iliotibial band friction syndrome(ITBS).Design:this was a two-arm randomized controlled trial with blinding of participants,outcome assessment and statistician.Setting:acupuncture-Moxibustion,Tuina and Orthopedics Department and the Orthopedics Department of Shanghai the Sixth People’s Hospital,June 2018 to January 2021.Participants:a total of 64 patients with iliotibial band friction syndrome(ITBS).Interventions:with a 1:1 ratio the patients were randomly assigned to either the conventional acupuncture group(CA,n=32) or the Relaxing Needling with Sharp Long Needle group(RNSLN,n=32).The CA group was treated by applying acupuncture at Jūliáo(居髎GB 29),Fēngshì(风市GB 31),Liángqiū(梁丘ST34),Yánglingquán(阳陵泉GB 34),and Ashi points(阿是穴)of the affected side,and the RNSLN group was treated by applying relaxing needling with sharp long needle at GB 29,ST 34,and Ashi points of the affected side.Both groups were treated once every other day,10 times as one course of treatment.Outcome measures:the treatment effect was evaluated after one course of treatment by comparing their KSS scores and VAS scores,as well as overall response rate.Results:Sixty-four patients were included in the final data analysis.There was no difference in KSS and VAS scores between the two groups before treatment(P>0.05).After treatment,the KSS score of the two groups was higher than that before treatment,the VAS score of the two groups was lower than that before treatment(P<0.05).The KSS score of the RNSLN group was higher than that of the CA group,and the VAS score of the RNSLN group was lower than that of the CA group(P<0.05).The overall response rate of the RNSLN group was 93.75%,higher than 75% of the control group(P<0.05).Each group has 1case of subcutaneous bleeding as adverse event.Conclusion:Relaxing Needling with Sharp Long Needle could be more beneficial for iliotibial band friction syndrome(ITBS) compared with conventional acupuncture,and more evidence is required for its effectiveness before introducing the approach widely to clinical practice.展开更多
文摘Objective: To observe the effect of acumoxi (acupuncture and moxibustion) on macrophage (Mφ)-lL1-Th net-work and hydroperitoneum hepatoma (H 22) metastasis in mice. Methods: A total of 36 BALB/ c male mice bearing H 22 are randomly divided into control, acupuncture and acumoxi groups with 12 cases in each group. In the later 2 groups, Dazhui (GV 14) and Guanyuan (CV 4) are punctured once daily, continuously for 18 days, and in acumoxi group, the two acupoints were also moxibustioned alternatively with moxa stick once every day. After killing the mice, the tissue samples of the 3 groups are treated routinely step by step and analyzed by means of colorimetric analysis for determining the phagocytic function of the macrophages; and the content of IL1 of the Mφ supernatant is assayed with serum plate agglutination (SPA)-Ig floral hoop method of T helper cell (Th) monoclonal antibody; the weight of the reniportal lymph node, the kidney and the lung, and the number of the cancerous nodes on the pulmonary surface are calculated. Results: After acupuncture and moxibustion treatment, the immunoregulatory network indices of acumoxi group increase obviously compared with those of control group(P<0.01), showing an anti-metastasis effect of acumoxi on H 22. Conclusion: Results of the present study and those of our former research prove that acupuncture and moxibustion can suppress the tumor growth and H 22 metastasis by the enhancement of the immunoregulatory network.
基金Supported by Shanghai Three-year Action Plan for Further Accelerating the Development of Traditional Chinese Medicine (2018-2020):ZY (2018-2020)-ZYJS-09Shanghai Three-year Action Plan for Further Accelerating the Development of Traditional Chinese Medicine (2018-2020):No.ZY(2018-2020)-FWTX-4020Shanghai Three-year Action Plan for Further Accelerating the Inheritance,Innovation,Development of Traditional Chinese Medicine (2021-2023):No.ZY (2021-2023)-0205-04。
文摘Objective:to observe the clinical effect of acupuncture by mángzhen huid(relaxing needling with sharp long needle) on iliotibial band friction syndrome(ITBS).Design:this was a two-arm randomized controlled trial with blinding of participants,outcome assessment and statistician.Setting:acupuncture-Moxibustion,Tuina and Orthopedics Department and the Orthopedics Department of Shanghai the Sixth People’s Hospital,June 2018 to January 2021.Participants:a total of 64 patients with iliotibial band friction syndrome(ITBS).Interventions:with a 1:1 ratio the patients were randomly assigned to either the conventional acupuncture group(CA,n=32) or the Relaxing Needling with Sharp Long Needle group(RNSLN,n=32).The CA group was treated by applying acupuncture at Jūliáo(居髎GB 29),Fēngshì(风市GB 31),Liángqiū(梁丘ST34),Yánglingquán(阳陵泉GB 34),and Ashi points(阿是穴)of the affected side,and the RNSLN group was treated by applying relaxing needling with sharp long needle at GB 29,ST 34,and Ashi points of the affected side.Both groups were treated once every other day,10 times as one course of treatment.Outcome measures:the treatment effect was evaluated after one course of treatment by comparing their KSS scores and VAS scores,as well as overall response rate.Results:Sixty-four patients were included in the final data analysis.There was no difference in KSS and VAS scores between the two groups before treatment(P>0.05).After treatment,the KSS score of the two groups was higher than that before treatment,the VAS score of the two groups was lower than that before treatment(P<0.05).The KSS score of the RNSLN group was higher than that of the CA group,and the VAS score of the RNSLN group was lower than that of the CA group(P<0.05).The overall response rate of the RNSLN group was 93.75%,higher than 75% of the control group(P<0.05).Each group has 1case of subcutaneous bleeding as adverse event.Conclusion:Relaxing Needling with Sharp Long Needle could be more beneficial for iliotibial band friction syndrome(ITBS) compared with conventional acupuncture,and more evidence is required for its effectiveness before introducing the approach widely to clinical practice.
