[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the...[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the obtained transformants were detected by PCR.[Result] A single square electric pulse could be applied to cell sample in 0.2 cm ET cuvettes by using a Bio-Rad Gene Pulser set at 150 V,25 ms at room temperature,but the transformation efficiency was very low.[Conclusion] This research may improve the transformation efficiency of T.maritima MS8.展开更多
In this paper,the main purpose is to analyze and research the characteristics of the geoelectric field observed data with a long time span and large amplitude abnormal change,at the Lhasa geomagnetic station( hereafte...In this paper,the main purpose is to analyze and research the characteristics of the geoelectric field observed data with a long time span and large amplitude abnormal change,at the Lhasa geomagnetic station( hereafter referred to as "Lhasa station "),before and after the Nepal M_S8. 1 strong earthquake,which occurred on April 25,2015. Based on the observation conditions,the observation system,and the observed data of Lhasa station preliminary discussed,the main characteristics of the abnormal change and evolution process are analyzed and studied,using the following two methods; the "synthesis energy accumulation"and the "power as MSA spectrum"analysis,from the two aspects of the"Time Domain"and"Frequency Domain. "The results show that the abnormal change of the geo-electric field observation of Lhasa station experienced a development stage following the process of "trend change- disturbance change- earthquake period-recovery period",and an evolution process of "low frequency change- high frequency change- smooth change- high frequency change ",before and after the Nepal M_S8. 1strong earthquake. Comprehensive analysis shows that the variation characteristics and evolution process of the geo-electric field at Lhasa station are basically consistent with the results of the relevant mechanism and phenomenon research. So far,this is valuable information with certain objectivity,which is typical and representative to reflect the whole process of the gestation, occurrence and complete development of such strongearthquakes.展开更多
In this paper,statistics are taken on the co-seismic response of underground fluid in Yunnan to the Nepal M_S8. 1 earthquake,and the co-seismic response characteristics of the water level and water temperature are ana...In this paper,statistics are taken on the co-seismic response of underground fluid in Yunnan to the Nepal M_S8. 1 earthquake,and the co-seismic response characteristics of the water level and water temperature are analyzed and summarized with the digital data. The results show that the Nepal M_S8. 1 earthquake had greater impact on the Yunnan region,and the macro and micro dynamics of fluids showed significant co-seismic response. The earthquake recording capacity of water level and temperature measurement is significantly higher than that of water radon and water quality to this large earthquake; the maximum amplitude and duration of co-seismic response of water level and water temperature vary greatly in different wells. The changing forms are dominated by fluctuation and step rise in water level,and a rising or falling restoration in water temperature. From the records of the main shock and the maximum strong aftershock,we can see that the greater magnitude of earthquake,the higher ratio of the occurrence of co-seismic response,and in the same well,the larger the response amplitude,as well as the longer the duration. The amplitude and duration of co-seismic response recorded by different instruments in a same well are different. Water temperature co-seismic response almost occurred in wells with water level response,indicating that the well water level and water temperature are closely related in co-seismic response,and the well water temperature seismic response was caused mainly by well water level seismic response.展开更多
基金Supported by Sub-Project of"973"(2004CB719600)National Natural Science Foundation Projects(30170511)~~
文摘[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the obtained transformants were detected by PCR.[Result] A single square electric pulse could be applied to cell sample in 0.2 cm ET cuvettes by using a Bio-Rad Gene Pulser set at 150 V,25 ms at room temperature,but the transformation efficiency was very low.[Conclusion] This research may improve the transformation efficiency of T.maritima MS8.
基金funded by the key projects off undamental Research projects in the Institute of Earthquake Science,CEA(Grant No:2013IES0101&2014IES0101)
文摘In this paper,the main purpose is to analyze and research the characteristics of the geoelectric field observed data with a long time span and large amplitude abnormal change,at the Lhasa geomagnetic station( hereafter referred to as "Lhasa station "),before and after the Nepal M_S8. 1 strong earthquake,which occurred on April 25,2015. Based on the observation conditions,the observation system,and the observed data of Lhasa station preliminary discussed,the main characteristics of the abnormal change and evolution process are analyzed and studied,using the following two methods; the "synthesis energy accumulation"and the "power as MSA spectrum"analysis,from the two aspects of the"Time Domain"and"Frequency Domain. "The results show that the abnormal change of the geo-electric field observation of Lhasa station experienced a development stage following the process of "trend change- disturbance change- earthquake period-recovery period",and an evolution process of "low frequency change- high frequency change- smooth change- high frequency change ",before and after the Nepal M_S8. 1strong earthquake. Comprehensive analysis shows that the variation characteristics and evolution process of the geo-electric field at Lhasa station are basically consistent with the results of the relevant mechanism and phenomenon research. So far,this is valuable information with certain objectivity,which is typical and representative to reflect the whole process of the gestation, occurrence and complete development of such strongearthquakes.
基金sponsored by the special fund of“A Study on Short-term Seismic Tracking of Strong Earthquakes in the Yunnan Area”of the“Ten Key Projects”in Yunnan Provincethe 2016 Earthquake Trend Tracking Task of China Earthquake Administration(2016010305)the 2015 Earthquake Trend Tracking Task of Earthquake Administration of Yunnan Province
文摘In this paper,statistics are taken on the co-seismic response of underground fluid in Yunnan to the Nepal M_S8. 1 earthquake,and the co-seismic response characteristics of the water level and water temperature are analyzed and summarized with the digital data. The results show that the Nepal M_S8. 1 earthquake had greater impact on the Yunnan region,and the macro and micro dynamics of fluids showed significant co-seismic response. The earthquake recording capacity of water level and temperature measurement is significantly higher than that of water radon and water quality to this large earthquake; the maximum amplitude and duration of co-seismic response of water level and water temperature vary greatly in different wells. The changing forms are dominated by fluctuation and step rise in water level,and a rising or falling restoration in water temperature. From the records of the main shock and the maximum strong aftershock,we can see that the greater magnitude of earthquake,the higher ratio of the occurrence of co-seismic response,and in the same well,the larger the response amplitude,as well as the longer the duration. The amplitude and duration of co-seismic response recorded by different instruments in a same well are different. Water temperature co-seismic response almost occurred in wells with water level response,indicating that the well water level and water temperature are closely related in co-seismic response,and the well water temperature seismic response was caused mainly by well water level seismic response.
